m0367  (New England Biolabs)


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  • 99
    Name:
    Blunt TA Ligase Master Mix
    Description:
    Blunt TA Ligase Master Mix 250 rxns
    Catalog Number:
    m0367l
    Price:
    392
    Size:
    250 rxns
    Category:
    DNA Ligases
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    Structured Review

    New England Biolabs m0367
    Blunt TA Ligase Master Mix
    Blunt TA Ligase Master Mix 250 rxns
    https://www.bioz.com/result/m0367/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m0367 - by Bioz Stars, 2020-09
    99/100 stars

    Related Products / Commonly Used Together

    blunt/ta ligase master mix
    ampure xp beads
    boligos
    1-nt ses

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    Related Articles

    Amplification:

    Article Title: Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices
    Article Snippet: .. For the samples with insufficient DNA requiring PCR amplification, additional steps between end-repair and sequencing adapter ligation included; PCR adapter ligation (Blunt/TA Ligase Master Mix, NEB) followed by SPRI bead purification; PCR amplification (Phusion High Fidelity PCR Master Mix, NEB) with 18 cycles (samples 229 and 249) or 24 cycles (samples 506 and 509) followed by additional SPRI bead purification. ..

    Ligation:

    Article Title: Nanopore Sequencing of a Forensic STR Multiplex Reveals Loci Suitable for Single-Contributor STR Profiling
    Article Snippet: .. Ligation of the barcodes was accomplished by adding 25 μL NEB Blunt/TA Ligase Master Mix and 2.5 μL Native Barcode to the sample. .. After an incubation step of 10 min at room temperature, the barcoded samples were cleaned using a volume of 1.8× AMPure XP beads.

    Article Title: One fly - one genome : Chromosome-scale genome assembly of a single outbred Drosophila melanogaster
    Article Snippet: .. End repair and A-tailing was performed using NEBNext Ultra II End Repair/dA-Tailing Module followed by ligation of Nextera adapters using NEB Blunt/TA Ligase Master Mix following the manufacturer’s protocol. .. The adaptor ligated sample was purified by SPRI beads at a 1:1 ratio and eluted in 50 ul of ultrapure water.

    Article Title: Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices
    Article Snippet: .. For the samples with insufficient DNA requiring PCR amplification, additional steps between end-repair and sequencing adapter ligation included; PCR adapter ligation (Blunt/TA Ligase Master Mix, NEB) followed by SPRI bead purification; PCR amplification (Phusion High Fidelity PCR Master Mix, NEB) with 18 cycles (samples 229 and 249) or 24 cycles (samples 506 and 509) followed by additional SPRI bead purification. ..

    Article Title: Polymerization retardation isothermal amplification (PRIA): a strategy enables sensitively quantify genome-wide 5-methylcytosine oxides rapidly on handy instruments with nanoscale sample input
    Article Snippet: .. Subsequently, they were reacted with the Blunt/TA Ligase Master Mix, the NEBNext Adaptor for Illumina and Ligation Enhancer at 20°C for 15 min before finally being treated with the USER™ enzyme at 37°C for 15 min. .. The adaptor-containing fragments were labeled with PHPA to obtain chemically labeled fragments, after which they were denatured at 95°C for 10 min and immediately transferred to ice to acquire single stranded DNA.

    Article Title: The structural variation landscape in 492 Atlantic salmon genomes
    Article Snippet: .. Native barcodes were ligated to end-repaired DNA using Blunt/TA Ligation Master Mix. .. Barcoded DNA was purified with AMPure XP beads and pooled in equimolar concentration to a total of 200 ng per library (∼0.2 pmol).

    Purification:

    Article Title: Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices
    Article Snippet: .. For the samples with insufficient DNA requiring PCR amplification, additional steps between end-repair and sequencing adapter ligation included; PCR adapter ligation (Blunt/TA Ligase Master Mix, NEB) followed by SPRI bead purification; PCR amplification (Phusion High Fidelity PCR Master Mix, NEB) with 18 cycles (samples 229 and 249) or 24 cycles (samples 506 and 509) followed by additional SPRI bead purification. ..

    Polymerase Chain Reaction:

    Article Title: Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices
    Article Snippet: .. For the samples with insufficient DNA requiring PCR amplification, additional steps between end-repair and sequencing adapter ligation included; PCR adapter ligation (Blunt/TA Ligase Master Mix, NEB) followed by SPRI bead purification; PCR amplification (Phusion High Fidelity PCR Master Mix, NEB) with 18 cycles (samples 229 and 249) or 24 cycles (samples 506 and 509) followed by additional SPRI bead purification. ..

    DNA Purification:

    Article Title: Cultivation and Transcriptional Analysis of a Canonical Nitrospira Under Stable Growth Conditions
    Article Snippet: .. Selected barcodes for each sample were ligated using the Blunt/TA Ligase Master Mix (New England Biolabs) and again followed by a DNA purification step. .. Thereafter, adapters were ligated using the NEBNext® Quick Ligation Module (New England Biolabs).

    Sequencing:

    Article Title: Real-time analysis of nanopore-based metagenomic sequencing from infected orthopaedic devices
    Article Snippet: .. For the samples with insufficient DNA requiring PCR amplification, additional steps between end-repair and sequencing adapter ligation included; PCR adapter ligation (Blunt/TA Ligase Master Mix, NEB) followed by SPRI bead purification; PCR amplification (Phusion High Fidelity PCR Master Mix, NEB) with 18 cycles (samples 229 and 249) or 24 cycles (samples 506 and 509) followed by additional SPRI bead purification. ..

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  • Team
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  • Bioz Stars
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  • 99
    New England Biolabs blunt ta ligase master mix
    Blunt Ta Ligase Master Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 173 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/blunt ta ligase master mix/product/New England Biolabs
    Average 99 stars, based on 173 article reviews
    Price from $9.99 to $1999.99
    blunt ta ligase master mix - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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