ahdi  (New England Biolabs)


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    Name:
    T4 Phage beta glucosyltransferase
    Description:
    T4 Phage beta glucosyltransferase 2 500 units
    Catalog Number:
    M0357L
    Price:
    360
    Category:
    Other Enzymes
    Size:
    2 500 units
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    Structured Review

    New England Biolabs ahdi
    T4 Phage beta glucosyltransferase
    T4 Phage beta glucosyltransferase 2 500 units
    https://www.bioz.com/result/ahdi/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ahdi - by Bioz Stars, 2021-05
    97/100 stars

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    Related Articles

    other:

    Article Title: Tissue-specific Distribution and Dynamic Changes of 5-Hydroxymethylcytosine in Mammalian Genomes *
    Article Snippet: Glucosylation of 5-hmC-containing Oligonucleotide Substrates with T4 β-Glucosyltransferase The hmC residues within the MspI site were glucosylated by incubating 200 pmol of DNA substrates with 1 μl (10 units) of T4 β-glucosyltransferase (β-GT) (NEB) for 1 h at 37 °C in a total 50-μl reaction containing 1× NEBuffer 4 supplemented with 0.1 mm UDP-Glc.

    Article Title: Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9
    Article Snippet: One microgram of T4(C), T4(HMC), or T4(glc-HMC) was digested with AluI (R0137s; NEB), MspJI (R0661S; NEB), or T4 phage β-glucosyltransferase (M0357S; NEB) in accordance with NEB-specified protocols.

    Real-time Polymerase Chain Reaction:

    Article Title: Aberrant transcriptomes and DNA methylomes define pathways that drive pathogenesis and loss of brain laterality/asymmetry in schizophrenia and bipolar disorder
    Article Snippet: 5-hmC is an intermediate formed during demethylation of methylated DNA, and has been reported to be abundant in human brain and to correspond to induction of gene expression ( ; ), even before its conversion to unmethylated cytosine ( ). .. In order to differentiate between 5-mC and 5-hmC, we used qPCR analysis of MspI and HpaII restricted DNA treated by T4 Phage β-glucosyltransferase (T4-BGT) with uridine diphosphoglucose (UDP-Glu) using the EpiMark 5-hmC and 5-mC Analysis Kit according to the instructions of the manufacturer (New England BioLabs, Cat# E3317S). ..

    Article Title: MicroRNA-29a induces loss of 5-hydroxymethylcytosine and promotes metastasis of hepatocellular carcinoma through a TET–SOCS1–MMP9 signaling axis
    Article Snippet: .. Glucosylation of genomic 5-hmC followed by methylation-sensitive qPCR Genomic DNA was treated with T4 Phageβ -glucosyltransferase (T4-BGT, New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instruction. ..

    Article Title: FGFR3△7–9 promotes tumor progression via the phosphorylation and destabilization of ten-eleven translocation-2 in human hepatocellular carcinoma
    Article Snippet: .. Quantification of 5-hmC levels in genomic DNA by methylation-sensitive qPCR Genomic DNA was incubated with T4 Phage β‐glucosyltransferase (New England Biolabs, Ipswich, MA) by following the manufacturer’s protocol. .. First, 100 ng of glucosylated genomic DNA was digested with Msp I, or without enzyme (mock) at 37 °C overnight and then incubated for 20 min at 80 °C for enzyme deactivation.

    Methylation:

    Article Title: MicroRNA-29a induces loss of 5-hydroxymethylcytosine and promotes metastasis of hepatocellular carcinoma through a TET–SOCS1–MMP9 signaling axis
    Article Snippet: .. Glucosylation of genomic 5-hmC followed by methylation-sensitive qPCR Genomic DNA was treated with T4 Phageβ -glucosyltransferase (T4-BGT, New England Biolabs, Ipswich, MA, USA) according to the manufacturer’s instruction. ..

    Article Title: FGFR3△7–9 promotes tumor progression via the phosphorylation and destabilization of ten-eleven translocation-2 in human hepatocellular carcinoma
    Article Snippet: .. Quantification of 5-hmC levels in genomic DNA by methylation-sensitive qPCR Genomic DNA was incubated with T4 Phage β‐glucosyltransferase (New England Biolabs, Ipswich, MA) by following the manufacturer’s protocol. .. First, 100 ng of glucosylated genomic DNA was digested with Msp I, or without enzyme (mock) at 37 °C overnight and then incubated for 20 min at 80 °C for enzyme deactivation.

    Incubation:

    Article Title: FGFR3△7–9 promotes tumor progression via the phosphorylation and destabilization of ten-eleven translocation-2 in human hepatocellular carcinoma
    Article Snippet: .. Quantification of 5-hmC levels in genomic DNA by methylation-sensitive qPCR Genomic DNA was incubated with T4 Phage β‐glucosyltransferase (New England Biolabs, Ipswich, MA) by following the manufacturer’s protocol. .. First, 100 ng of glucosylated genomic DNA was digested with Msp I, or without enzyme (mock) at 37 °C overnight and then incubated for 20 min at 80 °C for enzyme deactivation.

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    New England Biolabs t4 β glucosyltransferase β gt
    Validation of MspI and HpaII isoschizomer- and <t>β-glucosyltransferase-mediated</t> 5-hmC detection and quantitation. A , shown is unmethylated, methylated, and hydroxymethylated duplex DNAs with centrally located CCGG ( boxed ), where the internal C is either unmethylated C or 5-mC or 5-hmC (shown in gray ). B , shown is validation for locus-specific 5-hmC detection in a fixed amount of pre-mixed DNAs, as shown in A , using MspI in the presence of <t>β-GT</t> and cofactor UDP-Glc. The reaction products were subjected to qPCR, and the ratio between observed and expected 5-hmC is plotted. C , shown is similar validation for locus-specific methylcytosine ( 5-mC + 5-hmC ) detection in fixed amount of pre-mixed DNAs, as shown in A , using HpaII in the presence of β-GT and cofactor UDP-Glc. The reaction products were subjected to qPCR, and the ratio between observed and expected total methyl cytosine ( 5-mC + 5-hmC ) is plotted.
    T4 β Glucosyltransferase β Gt, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t4 β glucosyltransferase β gt/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t4 β glucosyltransferase β gt - by Bioz Stars, 2021-05
    97/100 stars
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    Validation of MspI and HpaII isoschizomer- and β-glucosyltransferase-mediated 5-hmC detection and quantitation. A , shown is unmethylated, methylated, and hydroxymethylated duplex DNAs with centrally located CCGG ( boxed ), where the internal C is either unmethylated C or 5-mC or 5-hmC (shown in gray ). B , shown is validation for locus-specific 5-hmC detection in a fixed amount of pre-mixed DNAs, as shown in A , using MspI in the presence of β-GT and cofactor UDP-Glc. The reaction products were subjected to qPCR, and the ratio between observed and expected 5-hmC is plotted. C , shown is similar validation for locus-specific methylcytosine ( 5-mC + 5-hmC ) detection in fixed amount of pre-mixed DNAs, as shown in A , using HpaII in the presence of β-GT and cofactor UDP-Glc. The reaction products were subjected to qPCR, and the ratio between observed and expected total methyl cytosine ( 5-mC + 5-hmC ) is plotted.

    Journal: The Journal of Biological Chemistry

    Article Title: Tissue-specific Distribution and Dynamic Changes of 5-Hydroxymethylcytosine in Mammalian Genomes *

    doi: 10.1074/jbc.M110.217083

    Figure Lengend Snippet: Validation of MspI and HpaII isoschizomer- and β-glucosyltransferase-mediated 5-hmC detection and quantitation. A , shown is unmethylated, methylated, and hydroxymethylated duplex DNAs with centrally located CCGG ( boxed ), where the internal C is either unmethylated C or 5-mC or 5-hmC (shown in gray ). B , shown is validation for locus-specific 5-hmC detection in a fixed amount of pre-mixed DNAs, as shown in A , using MspI in the presence of β-GT and cofactor UDP-Glc. The reaction products were subjected to qPCR, and the ratio between observed and expected 5-hmC is plotted. C , shown is similar validation for locus-specific methylcytosine ( 5-mC + 5-hmC ) detection in fixed amount of pre-mixed DNAs, as shown in A , using HpaII in the presence of β-GT and cofactor UDP-Glc. The reaction products were subjected to qPCR, and the ratio between observed and expected total methyl cytosine ( 5-mC + 5-hmC ) is plotted.

    Article Snippet: Glucosylation of 5-hmC-containing Oligonucleotide Substrates with T4 β-Glucosyltransferase The hmC residues within the MspI site were glucosylated by incubating 200 pmol of DNA substrates with 1 μl (10 units) of T4 β-glucosyltransferase (β-GT) (NEB) for 1 h at 37 °C in a total 50-μl reaction containing 1× NEBuffer 4 supplemented with 0.1 mm UDP-Glc.

    Techniques: Quantitation Assay, Methylation, Gas Chromatography, Real-time Polymerase Chain Reaction

    Characterization of phage T4 DNA modification. (A) Phage T4(glc-HMC), T4(HMC), and T4(C) DNA left untreated (−) or treated with (+) restriction enzymes AluI (top), which cleaves unmodified DNA; MspJI (middle), which cleaves HMC-containing DNA; or T4 glucosyltransferase (bottom), which increases the mobility of HMC-containing DNA by the addition of glucose groups. The arrows indicate the mobility shift due to glucose attachment. (B) Analysis of phage T4 DNA modification by single-molecule sequencing. Results are summarized for each genome by mapping IPD ratios at each base for each of the T4 strains studied. The coloration of each base is shown by the key at the bottom left. The T4 nucleotide sequence runs from top to bottom for each of the four genomes. The distance each colored point is displaced from the center indicates the IPD ratio (scale at bottom; leftward for the reverse strand, rightward for the forward strand). Examples of interpulse distances (indicative of modification) are shown to the right for a short segment of the T4 genome. Bars indicate the magnitude of the IPD ratio (upward for the forward strand and downward for the reverse strand). A 5′ GATC 3′ site of DAM methylation is highlighted in yellow. (C) Violin plot showing IPD ratios of A residues at 5′ GATC 3′ sequences.

    Journal: mBio

    Article Title: Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9

    doi: 10.1128/mBio.00648-15

    Figure Lengend Snippet: Characterization of phage T4 DNA modification. (A) Phage T4(glc-HMC), T4(HMC), and T4(C) DNA left untreated (−) or treated with (+) restriction enzymes AluI (top), which cleaves unmodified DNA; MspJI (middle), which cleaves HMC-containing DNA; or T4 glucosyltransferase (bottom), which increases the mobility of HMC-containing DNA by the addition of glucose groups. The arrows indicate the mobility shift due to glucose attachment. (B) Analysis of phage T4 DNA modification by single-molecule sequencing. Results are summarized for each genome by mapping IPD ratios at each base for each of the T4 strains studied. The coloration of each base is shown by the key at the bottom left. The T4 nucleotide sequence runs from top to bottom for each of the four genomes. The distance each colored point is displaced from the center indicates the IPD ratio (scale at bottom; leftward for the reverse strand, rightward for the forward strand). Examples of interpulse distances (indicative of modification) are shown to the right for a short segment of the T4 genome. Bars indicate the magnitude of the IPD ratio (upward for the forward strand and downward for the reverse strand). A 5′ GATC 3′ site of DAM methylation is highlighted in yellow. (C) Violin plot showing IPD ratios of A residues at 5′ GATC 3′ sequences.

    Article Snippet: One microgram of T4(C), T4(HMC), or T4(glc-HMC) was digested with AluI (R0137s; NEB), MspJI (R0661S; NEB), or T4 phage β-glucosyltransferase (M0357S; NEB) in accordance with NEB-specified protocols.

    Techniques: Modification, Mobility Shift, Sequencing, Methylation

    Development and validation of bisulfite ACE-seq for whole genome 5hmC analysis. a, Workflow comparison illustrating stepwise differences in cytosine modification status and 5hmC- protection between standard and bisulfite ACE-seq, respectively. b, Bar plots showing the cytosine modification levels in spike-in controls (methylated lambda and mutant T4 phage genomes) analyzed by BS-seq (red), bisulfite ACE-seq (dark blue) and standard ACE-seq (light blue). All methylated (5mCG) and unmodified cytosines (CHG/CHH) in the lambda phage genome should be deaminated in standard and bisulfite ACE-seq. Conversely, 5hmC should be protected at every cytosine position in the mutant T4 (5hmC only) phage genome for all three techniques. The mean values of multiple replicates are shown on the right. Bars and error bars represent the mean ± s.d. of results from multiple experiments (number of replicates are indicated on the top).

    Journal: bioRxiv

    Article Title: Quantitative single cell 5hmC sequencing reveals non-canonical gene regulation by non-CG hydroxymethylation

    doi: 10.1101/2021.03.23.434325

    Figure Lengend Snippet: Development and validation of bisulfite ACE-seq for whole genome 5hmC analysis. a, Workflow comparison illustrating stepwise differences in cytosine modification status and 5hmC- protection between standard and bisulfite ACE-seq, respectively. b, Bar plots showing the cytosine modification levels in spike-in controls (methylated lambda and mutant T4 phage genomes) analyzed by BS-seq (red), bisulfite ACE-seq (dark blue) and standard ACE-seq (light blue). All methylated (5mCG) and unmodified cytosines (CHG/CHH) in the lambda phage genome should be deaminated in standard and bisulfite ACE-seq. Conversely, 5hmC should be protected at every cytosine position in the mutant T4 (5hmC only) phage genome for all three techniques. The mean values of multiple replicates are shown on the right. Bars and error bars represent the mean ± s.d. of results from multiple experiments (number of replicates are indicated on the top).

    Article Snippet: In a total volume of 5uL, the gDNA mixture was glucosylated using UDP-glucose and T4 ꞵ-glucosyltransferase (T4-ꞵGT, NEB M0357S) at 37°C for 1h.

    Techniques: Modification, Methylation, Mutagenesis