pyrophosphohydrolase  (New England Biolabs)


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    Name:
    RNA 5’ Pyrophosphohydrolase RppH
    Description:
    RNA 5 Pyrophosphohydrolase RppH 200 units
    Catalog Number:
    M0356S
    Price:
    93
    Category:
    Other Enzymes
    Size:
    200 units
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    Structured Review

    New England Biolabs pyrophosphohydrolase
    RNA 5’ Pyrophosphohydrolase RppH
    RNA 5 Pyrophosphohydrolase RppH 200 units
    https://www.bioz.com/result/pyrophosphohydrolase/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pyrophosphohydrolase - by Bioz Stars, 2021-06
    99/100 stars

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    Related Articles

    In Vitro:

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes
    Article Snippet: The 111 nt in vitro transcription RNA was purified using a Zymo Concentrator-5 kit (Zymo Research, Irvine, CA, USA), all other RNAs were purified by ethanol precipitation. .. The 111 nt in vitro transcribed RNA was pre-treated to modify the 5′ ends as follows: (i) 1 μg of RNA was incubated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C; (ii) 1 μg of RNA was incubated with 1 μl of VCE (NEB) for 30 min at 37°C; or (iii) 1 μg of RNA was treated with RppH for 1 h at 37°C and purified by ethanol precipitation, followed by incubation with VCE for 30 min at 37°C. .. All samples were purified by ethanol precipitation and evaluated using a Nano Drop (Thermo Fisher Scientific).

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes
    Article Snippet: The 111 nt in vitro transcription RNA was purified using a Zymo Concentrator-5 kit (Zymo Research, Irvine, CA, USA), all other RNAs were purified by ethanol precipitation. .. Different treatments of the 111 nt in vitro transcribed RNA The 111 nt in vitro transcribed RNA was pre-treated to modify the 5′ ends as follows: (i) 1 μg of RNA was incubated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C; (ii) 1 μg of RNA was incubated with 1 μl of VCE (NEB) for 30 min at 37°C; or (iii) 1 μg of RNA was treated with RppH for 1 h at 37°C and purified by ethanol precipitation, followed by incubation with VCE for 30 min at 37°C. .. All samples were purified by ethanol precipitation and evaluated using a Nano Drop (Thermo Fisher Scientific).

    Incubation:

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes
    Article Snippet: The 111 nt in vitro transcription RNA was purified using a Zymo Concentrator-5 kit (Zymo Research, Irvine, CA, USA), all other RNAs were purified by ethanol precipitation. .. The 111 nt in vitro transcribed RNA was pre-treated to modify the 5′ ends as follows: (i) 1 μg of RNA was incubated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C; (ii) 1 μg of RNA was incubated with 1 μl of VCE (NEB) for 30 min at 37°C; or (iii) 1 μg of RNA was treated with RppH for 1 h at 37°C and purified by ethanol precipitation, followed by incubation with VCE for 30 min at 37°C. .. All samples were purified by ethanol precipitation and evaluated using a Nano Drop (Thermo Fisher Scientific).

    Article Title: De Novo RNA Synthesis by RNA-Dependent RNA Polymerase Activity of Telomerase Reverse Transcriptase
    Article Snippet: Ten batches of IP-RdRP products and the 3′ SR adaptor for Illumina sequencing were mixed and denatured at 98°C for 1 min. .. Thereafter, 3′ adaptor ligation was performed using the denatured mixture, 3′ ligation reaction buffer, and 3′ ligation enzyme mix at 25°C for 1 h. Half the amount (0.5 μl) of SR RT primer for Illumina sequencing was added to the mixture, and then the mixture was denatured at 98°C for 1 min. To remove pyrophosphate from the 5′ ends of triphosphorylated RNA, the RNA mixture was incubated with RNA 5′ pyrophosphohydrolase (RppH) (New England BioLabs) and simultaneously incubated with 5′ SR adaptor for Illumina sequencing, 5′ ligation reaction buffer, and 5′ ligase enzyme mix for 5′ adaptor ligation at 25°C for 1 h. After the rest of the SR RT primer for Illumina sequencing was added, the mixture was denatured at 70°C for 2 min, and then reverse transcription and 12 PCR cycles were performed according to the manufacturer's protocol. .. The PCR products were purified by PAGE extraction, and six additional PCR cycles were performed to obtain sufficient amounts of products for sequencing with a HiSeq 2000 instrument.

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes
    Article Snippet: The 111 nt in vitro transcription RNA was purified using a Zymo Concentrator-5 kit (Zymo Research, Irvine, CA, USA), all other RNAs were purified by ethanol precipitation. .. Different treatments of the 111 nt in vitro transcribed RNA The 111 nt in vitro transcribed RNA was pre-treated to modify the 5′ ends as follows: (i) 1 μg of RNA was incubated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C; (ii) 1 μg of RNA was incubated with 1 μl of VCE (NEB) for 30 min at 37°C; or (iii) 1 μg of RNA was treated with RppH for 1 h at 37°C and purified by ethanol precipitation, followed by incubation with VCE for 30 min at 37°C. .. All samples were purified by ethanol precipitation and evaluated using a Nano Drop (Thermo Fisher Scientific).

    Purification:

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes
    Article Snippet: The 111 nt in vitro transcription RNA was purified using a Zymo Concentrator-5 kit (Zymo Research, Irvine, CA, USA), all other RNAs were purified by ethanol precipitation. .. The 111 nt in vitro transcribed RNA was pre-treated to modify the 5′ ends as follows: (i) 1 μg of RNA was incubated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C; (ii) 1 μg of RNA was incubated with 1 μl of VCE (NEB) for 30 min at 37°C; or (iii) 1 μg of RNA was treated with RppH for 1 h at 37°C and purified by ethanol precipitation, followed by incubation with VCE for 30 min at 37°C. .. All samples were purified by ethanol precipitation and evaluated using a Nano Drop (Thermo Fisher Scientific).

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes
    Article Snippet: RLM-RACE RLM-RACE was performed as described previously with modification ( ). .. Briefly, bacterial RNA was treated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C, and purified by ethanol precipitation. .. The treated RNA was ligated to a synthetic RNA adaptor with T4 RNA ligase 1 (Thermo Fisher Scientific) for 30 min at 37°C.

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes
    Article Snippet: The 111 nt in vitro transcription RNA was purified using a Zymo Concentrator-5 kit (Zymo Research, Irvine, CA, USA), all other RNAs were purified by ethanol precipitation. .. Different treatments of the 111 nt in vitro transcribed RNA The 111 nt in vitro transcribed RNA was pre-treated to modify the 5′ ends as follows: (i) 1 μg of RNA was incubated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C; (ii) 1 μg of RNA was incubated with 1 μl of VCE (NEB) for 30 min at 37°C; or (iii) 1 μg of RNA was treated with RppH for 1 h at 37°C and purified by ethanol precipitation, followed by incubation with VCE for 30 min at 37°C. .. All samples were purified by ethanol precipitation and evaluated using a Nano Drop (Thermo Fisher Scientific).

    Ethanol Precipitation:

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes
    Article Snippet: The 111 nt in vitro transcription RNA was purified using a Zymo Concentrator-5 kit (Zymo Research, Irvine, CA, USA), all other RNAs were purified by ethanol precipitation. .. The 111 nt in vitro transcribed RNA was pre-treated to modify the 5′ ends as follows: (i) 1 μg of RNA was incubated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C; (ii) 1 μg of RNA was incubated with 1 μl of VCE (NEB) for 30 min at 37°C; or (iii) 1 μg of RNA was treated with RppH for 1 h at 37°C and purified by ethanol precipitation, followed by incubation with VCE for 30 min at 37°C. .. All samples were purified by ethanol precipitation and evaluated using a Nano Drop (Thermo Fisher Scientific).

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes
    Article Snippet: RLM-RACE RLM-RACE was performed as described previously with modification ( ). .. Briefly, bacterial RNA was treated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C, and purified by ethanol precipitation. .. The treated RNA was ligated to a synthetic RNA adaptor with T4 RNA ligase 1 (Thermo Fisher Scientific) for 30 min at 37°C.

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes
    Article Snippet: The 111 nt in vitro transcription RNA was purified using a Zymo Concentrator-5 kit (Zymo Research, Irvine, CA, USA), all other RNAs were purified by ethanol precipitation. .. Different treatments of the 111 nt in vitro transcribed RNA The 111 nt in vitro transcribed RNA was pre-treated to modify the 5′ ends as follows: (i) 1 μg of RNA was incubated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C; (ii) 1 μg of RNA was incubated with 1 μl of VCE (NEB) for 30 min at 37°C; or (iii) 1 μg of RNA was treated with RppH for 1 h at 37°C and purified by ethanol precipitation, followed by incubation with VCE for 30 min at 37°C. .. All samples were purified by ethanol precipitation and evaluated using a Nano Drop (Thermo Fisher Scientific).

    Expressing:

    Article Title: A Structure-Based Model for the Complete Transcription Cycle of Influenza Polymerase.
    Article Snippet: Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). .. Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). ..

    Recombinant:

    Article Title: A Structure-Based Model for the Complete Transcription Cycle of Influenza Polymerase.
    Article Snippet: Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). .. Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). ..

    Protease Inhibitor:

    Article Title: A Structure-Based Model for the Complete Transcription Cycle of Influenza Polymerase.
    Article Snippet: Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). .. Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). ..

    Staining:

    Article Title: A Structure-Based Model for the Complete Transcription Cycle of Influenza Polymerase.
    Article Snippet: Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). .. Influenza polymerase uses unique mechanisms to synthesize capped and polyadenylated mRNAs from the genomic viral RNA (vRNA) template, which is packaged inside ribonucleoprotein particles (vRNPs). ..

    Ligation:

    Article Title: De Novo RNA Synthesis by RNA-Dependent RNA Polymerase Activity of Telomerase Reverse Transcriptase
    Article Snippet: Ten batches of IP-RdRP products and the 3′ SR adaptor for Illumina sequencing were mixed and denatured at 98°C for 1 min. .. Thereafter, 3′ adaptor ligation was performed using the denatured mixture, 3′ ligation reaction buffer, and 3′ ligation enzyme mix at 25°C for 1 h. Half the amount (0.5 μl) of SR RT primer for Illumina sequencing was added to the mixture, and then the mixture was denatured at 98°C for 1 min. To remove pyrophosphate from the 5′ ends of triphosphorylated RNA, the RNA mixture was incubated with RNA 5′ pyrophosphohydrolase (RppH) (New England BioLabs) and simultaneously incubated with 5′ SR adaptor for Illumina sequencing, 5′ ligation reaction buffer, and 5′ ligase enzyme mix for 5′ adaptor ligation at 25°C for 1 h. After the rest of the SR RT primer for Illumina sequencing was added, the mixture was denatured at 70°C for 2 min, and then reverse transcription and 12 PCR cycles were performed according to the manufacturer's protocol. .. The PCR products were purified by PAGE extraction, and six additional PCR cycles were performed to obtain sufficient amounts of products for sequencing with a HiSeq 2000 instrument.

    Sequencing:

    Article Title: De Novo RNA Synthesis by RNA-Dependent RNA Polymerase Activity of Telomerase Reverse Transcriptase
    Article Snippet: Ten batches of IP-RdRP products and the 3′ SR adaptor for Illumina sequencing were mixed and denatured at 98°C for 1 min. .. Thereafter, 3′ adaptor ligation was performed using the denatured mixture, 3′ ligation reaction buffer, and 3′ ligation enzyme mix at 25°C for 1 h. Half the amount (0.5 μl) of SR RT primer for Illumina sequencing was added to the mixture, and then the mixture was denatured at 98°C for 1 min. To remove pyrophosphate from the 5′ ends of triphosphorylated RNA, the RNA mixture was incubated with RNA 5′ pyrophosphohydrolase (RppH) (New England BioLabs) and simultaneously incubated with 5′ SR adaptor for Illumina sequencing, 5′ ligation reaction buffer, and 5′ ligase enzyme mix for 5′ adaptor ligation at 25°C for 1 h. After the rest of the SR RT primer for Illumina sequencing was added, the mixture was denatured at 70°C for 2 min, and then reverse transcription and 12 PCR cycles were performed according to the manufacturer's protocol. .. The PCR products were purified by PAGE extraction, and six additional PCR cycles were performed to obtain sufficient amounts of products for sequencing with a HiSeq 2000 instrument.

    Polymerase Chain Reaction:

    Article Title: De Novo RNA Synthesis by RNA-Dependent RNA Polymerase Activity of Telomerase Reverse Transcriptase
    Article Snippet: Ten batches of IP-RdRP products and the 3′ SR adaptor for Illumina sequencing were mixed and denatured at 98°C for 1 min. .. Thereafter, 3′ adaptor ligation was performed using the denatured mixture, 3′ ligation reaction buffer, and 3′ ligation enzyme mix at 25°C for 1 h. Half the amount (0.5 μl) of SR RT primer for Illumina sequencing was added to the mixture, and then the mixture was denatured at 98°C for 1 min. To remove pyrophosphate from the 5′ ends of triphosphorylated RNA, the RNA mixture was incubated with RNA 5′ pyrophosphohydrolase (RppH) (New England BioLabs) and simultaneously incubated with 5′ SR adaptor for Illumina sequencing, 5′ ligation reaction buffer, and 5′ ligase enzyme mix for 5′ adaptor ligation at 25°C for 1 h. After the rest of the SR RT primer for Illumina sequencing was added, the mixture was denatured at 70°C for 2 min, and then reverse transcription and 12 PCR cycles were performed according to the manufacturer's protocol. .. The PCR products were purified by PAGE extraction, and six additional PCR cycles were performed to obtain sufficient amounts of products for sequencing with a HiSeq 2000 instrument.

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  • 99
    New England Biolabs rna 5 pyrophosphohydrolase rpph
    5′ RACE mapping of B. melitensis 16M protein-coding genes. ( A ) Nest-PCR products of 5′ RACE starting from B. melitensis 16M total RNA. ompA , outer membrane protein A (337 bp); rne , an endoribonuclease (286 bp); <t>rppH</t> , RNA <t>pyrophosphohydrolase</t> (400 bp). Lane M, DL2000 DNA Marker (Takara); lane 1, first-round PCR products; lane 2, second-round PCR products. ( B ) The sequencing result of the second-round PCR products.
    Rna 5 Pyrophosphohydrolase Rpph, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna 5 pyrophosphohydrolase rpph/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rna 5 pyrophosphohydrolase rpph - by Bioz Stars, 2021-06
    99/100 stars
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    5′ RACE mapping of B. melitensis 16M protein-coding genes. ( A ) Nest-PCR products of 5′ RACE starting from B. melitensis 16M total RNA. ompA , outer membrane protein A (337 bp); rne , an endoribonuclease (286 bp); rppH , RNA pyrophosphohydrolase (400 bp). Lane M, DL2000 DNA Marker (Takara); lane 1, first-round PCR products; lane 2, second-round PCR products. ( B ) The sequencing result of the second-round PCR products.

    Journal: Nucleic Acids Research

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes

    doi: 10.1093/nar/gky739

    Figure Lengend Snippet: 5′ RACE mapping of B. melitensis 16M protein-coding genes. ( A ) Nest-PCR products of 5′ RACE starting from B. melitensis 16M total RNA. ompA , outer membrane protein A (337 bp); rne , an endoribonuclease (286 bp); rppH , RNA pyrophosphohydrolase (400 bp). Lane M, DL2000 DNA Marker (Takara); lane 1, first-round PCR products; lane 2, second-round PCR products. ( B ) The sequencing result of the second-round PCR products.

    Article Snippet: Briefly, bacterial RNA was treated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C, and purified by ethanol precipitation.

    Techniques: Polymerase Chain Reaction, Marker, Sequencing

    5′ RACE mapping of B. melitensis 16M protein-coding genes. ( A ) Nest-PCR products of 5′ RACE starting from B. melitensis 16M total RNA. ompA , outer membrane protein A (337 bp); rne , an endoribonuclease (286 bp); rppH , RNA pyrophosphohydrolase (400 bp). Lane M, DL2000 DNA Marker (Takara); lane 1, first-round PCR products; lane 2, second-round PCR products. ( B ) The sequencing result of the second-round PCR products.

    Journal: Nucleic Acids Research

    Article Title: Capping-RACE: a simple, accurate, and sensitive 5′ RACE method for use in prokaryotes

    doi: 10.1093/nar/gky739

    Figure Lengend Snippet: 5′ RACE mapping of B. melitensis 16M protein-coding genes. ( A ) Nest-PCR products of 5′ RACE starting from B. melitensis 16M total RNA. ompA , outer membrane protein A (337 bp); rne , an endoribonuclease (286 bp); rppH , RNA pyrophosphohydrolase (400 bp). Lane M, DL2000 DNA Marker (Takara); lane 1, first-round PCR products; lane 2, second-round PCR products. ( B ) The sequencing result of the second-round PCR products.

    Article Snippet: The 111 nt in vitro transcribed RNA was pre-treated to modify the 5′ ends as follows: (i) 1 μg of RNA was incubated with 1 μl of RNA 5′ pyrophosphohydrolase (RppH) (NEB, Ipswich, MA, USA) for 1 h at 37°C; (ii) 1 μg of RNA was incubated with 1 μl of VCE (NEB) for 30 min at 37°C; or (iii) 1 μg of RNA was treated with RppH for 1 h at 37°C and purified by ethanol precipitation, followed by incubation with VCE for 30 min at 37°C.

    Techniques: Polymerase Chain Reaction, Marker, Sequencing

    Integrator‐mediated cleavage of nascent piRNA precursors generates short‐capped RNAs Model for Integrator cleavage of nascent piRNA precursors associated with promoter‐proximal Pol II. Cleavage results in the production of a short‐capped RNA, and a 3′ degradation fragment. Signal of 5′ P small RNA 5′ ends mapping to piRNA loci as a function of the distance to piRNA TSSs, after removal of reads corresponding to mature 21U‐RNAs (see Materials and Methods). The signal is normalized to counts per million of non‐structural mapped reads. A peak of 5′ P ends centred at +38 from piRNA TSSs is observed. Distributions of counts per million of 5′ P ends mapping from +25 to +50 of piRNA TSSs across the different genotypes and conditions. Two independent biological replicates are shown. Boxplots show the interquartile range with a line at the median; the whiskers extend to the greatest point no more than 1.5 times the interquartile range. Length distribution of putative 3′ degradation fragments with 5′ P ends mapping at +28‐+58 of the 5′ U of annotated 21U‐RNAs. Number of loci with detected cleavage fragments in N2 empty vector nematodes across percentile bins of increasing termination signal strength (top panel). Distributions of fragment read counts per locus for detected loci across percentile bins of increasing termination signal strength (bottom panel). Boxplots show the interquartile range with a line at the median; the whiskers extend to the greatest point no more than 1.5 times the interquartile range. The data shown here correspond to one of two N2 EV replicates, see Appendix Fig   S6  for this analysis across replicates and genotypes.

    Journal: The EMBO Journal

    Article Title: Integrator is recruited to promoter‐proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors

    doi: 10.15252/embj.2020105564

    Figure Lengend Snippet: Integrator‐mediated cleavage of nascent piRNA precursors generates short‐capped RNAs Model for Integrator cleavage of nascent piRNA precursors associated with promoter‐proximal Pol II. Cleavage results in the production of a short‐capped RNA, and a 3′ degradation fragment. Signal of 5′ P small RNA 5′ ends mapping to piRNA loci as a function of the distance to piRNA TSSs, after removal of reads corresponding to mature 21U‐RNAs (see Materials and Methods). The signal is normalized to counts per million of non‐structural mapped reads. A peak of 5′ P ends centred at +38 from piRNA TSSs is observed. Distributions of counts per million of 5′ P ends mapping from +25 to +50 of piRNA TSSs across the different genotypes and conditions. Two independent biological replicates are shown. Boxplots show the interquartile range with a line at the median; the whiskers extend to the greatest point no more than 1.5 times the interquartile range. Length distribution of putative 3′ degradation fragments with 5′ P ends mapping at +28‐+58 of the 5′ U of annotated 21U‐RNAs. Number of loci with detected cleavage fragments in N2 empty vector nematodes across percentile bins of increasing termination signal strength (top panel). Distributions of fragment read counts per locus for detected loci across percentile bins of increasing termination signal strength (bottom panel). Boxplots show the interquartile range with a line at the median; the whiskers extend to the greatest point no more than 1.5 times the interquartile range. The data shown here correspond to one of two N2 EV replicates, see Appendix Fig  S6 for this analysis across replicates and genotypes.

    Article Snippet: The next day, RNA was resuspended and treated with 7.5 units (1.5 µl) RNA 5′ pyrophosphohydrolase (NEB) for 1 h at 37°C in a total volume of 20 µl.

    Techniques: Plasmid Preparation