recaf  (New England Biolabs)


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  • 79
    Name:
    RecAf
    Description:
    RecAf 1 000 ug
    Catalog Number:
    m0355l
    Price:
    284
    Size:
    1 000 ug
    Category:
    Other Endonucleases
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    Structured Review

    New England Biolabs recaf
    RecAf
    RecAf 1 000 ug
    https://www.bioz.com/result/recaf/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recaf - by Bioz Stars, 2020-01
    79/100 stars

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    Related Articles

    In Vitro:

    Article Title: A Small-Molecule Inducible Synthetic Circuit for Control of the SOS Gene Network without DNA Damage
    Article Snippet: Paragraph title: In Vitro Protein Analysis ... To qualitatively examine LexA autocleavage, 5 μM purified LexA variants were incubated in Buffer A with 20 μg/mL RecAf (New England Biolabs, NEB) preactivated by coincubation with 10 μM ATPγS and 10 μM ssDNA, either alone or in the presence of 10 μM SOS operator or scrambled operator DNA.

    Purification:

    Article Title: A Small-Molecule Inducible Synthetic Circuit for Control of the SOS Gene Network without DNA Damage
    Article Snippet: .. To qualitatively examine LexA autocleavage, 5 μM purified LexA variants were incubated in Buffer A with 20 μg/mL RecAf (New England Biolabs, NEB) preactivated by coincubation with 10 μM ATPγS and 10 μM ssDNA, either alone or in the presence of 10 μM SOS operator or scrambled operator DNA. ..

    Acrylamide Gel Assay:

    Article Title: A Small-Molecule Inducible Synthetic Circuit for Control of the SOS Gene Network without DNA Damage
    Article Snippet: Serial dilutions of LexA were incubated with 100 pM of the radiolabeled duplex in 70 mM Tris (pH 7.6), 10 mM MgCl2 (Buffer A) at 25 °C either alone or with a 1000-fold excess (1 ng/μL) of unlabeled sonicated salmon sperm DNA, and samples were separated on a native 8% acrylamide gel at 25 °C in 0.5× Tris/Borate/EDTA buffer. .. To qualitatively examine LexA autocleavage, 5 μM purified LexA variants were incubated in Buffer A with 20 μg/mL RecAf (New England Biolabs, NEB) preactivated by coincubation with 10 μM ATPγS and 10 μM ssDNA, either alone or in the presence of 10 μM SOS operator or scrambled operator DNA.

    Incubation:

    Article Title: A Small-Molecule Inducible Synthetic Circuit for Control of the SOS Gene Network without DNA Damage
    Article Snippet: .. To qualitatively examine LexA autocleavage, 5 μM purified LexA variants were incubated in Buffer A with 20 μg/mL RecAf (New England Biolabs, NEB) preactivated by coincubation with 10 μM ATPγS and 10 μM ssDNA, either alone or in the presence of 10 μM SOS operator or scrambled operator DNA. ..

    Sequencing:

    Article Title: A Small-Molecule Inducible Synthetic Circuit for Control of the SOS Gene Network without DNA Damage
    Article Snippet: 44 bp oligonucleotides containing either the SOS consensus sequence or a scrambled control were 5′-radiolabeled with 32 P using T4 polynucleotide kinase, then annealed to their complementary unlabeled strand. .. To qualitatively examine LexA autocleavage, 5 μM purified LexA variants were incubated in Buffer A with 20 μg/mL RecAf (New England Biolabs, NEB) preactivated by coincubation with 10 μM ATPγS and 10 μM ssDNA, either alone or in the presence of 10 μM SOS operator or scrambled operator DNA.

    Sonication:

    Article Title: A Small-Molecule Inducible Synthetic Circuit for Control of the SOS Gene Network without DNA Damage
    Article Snippet: Serial dilutions of LexA were incubated with 100 pM of the radiolabeled duplex in 70 mM Tris (pH 7.6), 10 mM MgCl2 (Buffer A) at 25 °C either alone or with a 1000-fold excess (1 ng/μL) of unlabeled sonicated salmon sperm DNA, and samples were separated on a native 8% acrylamide gel at 25 °C in 0.5× Tris/Borate/EDTA buffer. .. To qualitatively examine LexA autocleavage, 5 μM purified LexA variants were incubated in Buffer A with 20 μg/mL RecAf (New England Biolabs, NEB) preactivated by coincubation with 10 μM ATPγS and 10 μM ssDNA, either alone or in the presence of 10 μM SOS operator or scrambled operator DNA.

    Binding Assay:

    Article Title: A Small-Molecule Inducible Synthetic Circuit for Control of the SOS Gene Network without DNA Damage
    Article Snippet: The data were fit to one site specific binding via Prism software. .. To qualitatively examine LexA autocleavage, 5 μM purified LexA variants were incubated in Buffer A with 20 μg/mL RecAf (New England Biolabs, NEB) preactivated by coincubation with 10 μM ATPγS and 10 μM ssDNA, either alone or in the presence of 10 μM SOS operator or scrambled operator DNA.

    Software:

    Article Title: A Small-Molecule Inducible Synthetic Circuit for Control of the SOS Gene Network without DNA Damage
    Article Snippet: The data were fit to one site specific binding via Prism software. .. To qualitatively examine LexA autocleavage, 5 μM purified LexA variants were incubated in Buffer A with 20 μg/mL RecAf (New England Biolabs, NEB) preactivated by coincubation with 10 μM ATPγS and 10 μM ssDNA, either alone or in the presence of 10 μM SOS operator or scrambled operator DNA.

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  • 79
    New England Biolabs recaf
    Recaf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recaf/product/New England Biolabs
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    recaf - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

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