hemo klentaq  (New England Biolabs)


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  • 96
    Name:
    Hemo KlenTaq
    Description:
    Hemo KlenTaq 1 000 rxns
    Catalog Number:
    m0332l
    Price:
    432
    Size:
    1 000 rxns
    Category:
    Thermostable DNA Polymerases
    Buy from Supplier


    Structured Review

    New England Biolabs hemo klentaq
    Hemo KlenTaq
    Hemo KlenTaq 1 000 rxns
    https://www.bioz.com/result/hemo klentaq/product/New England Biolabs
    Average 96 stars, based on 111 article reviews
    Price from $9.99 to $1999.99
    hemo klentaq - by Bioz Stars, 2020-07
    96/100 stars

    Images

    1) Product Images from "Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation"

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

    Journal: The Journal of Molecular Diagnostics : JMD

    doi: 10.1016/j.jmoldx.2011.09.002

    Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star
    Figure Legend Snippet: Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    2) Product Images from "Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation"

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

    Journal: The Journal of Molecular Diagnostics : JMD

    doi: 10.1016/j.jmoldx.2011.09.002

    Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star
    Figure Legend Snippet: Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    3) Product Images from "Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation"

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

    Journal: The Journal of Molecular Diagnostics : JMD

    doi: 10.1016/j.jmoldx.2011.09.002

    Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star
    Figure Legend Snippet: Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    4) Product Images from "Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation"

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

    Journal: The Journal of Molecular Diagnostics : JMD

    doi: 10.1016/j.jmoldx.2011.09.002

    Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star
    Figure Legend Snippet: Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    5) Product Images from "Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation"

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

    Journal: The Journal of Molecular Diagnostics : JMD

    doi: 10.1016/j.jmoldx.2011.09.002

    Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star
    Figure Legend Snippet: Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    6) Product Images from "Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation"

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

    Journal: The Journal of Molecular Diagnostics : JMD

    doi: 10.1016/j.jmoldx.2011.09.002

    Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star
    Figure Legend Snippet: Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    7) Product Images from "Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation"

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

    Journal: The Journal of Molecular Diagnostics : JMD

    doi: 10.1016/j.jmoldx.2011.09.002

    Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star
    Figure Legend Snippet: Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    8) Product Images from "Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation"

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

    Journal: The Journal of Molecular Diagnostics : JMD

    doi: 10.1016/j.jmoldx.2011.09.002

    Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star
    Figure Legend Snippet: Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    9) Product Images from "Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation"

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

    Journal: The Journal of Molecular Diagnostics : JMD

    doi: 10.1016/j.jmoldx.2011.09.002

    Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star
    Figure Legend Snippet: Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    10) Product Images from "Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation"

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

    Journal: The Journal of Molecular Diagnostics : JMD

    doi: 10.1016/j.jmoldx.2011.09.002

    Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star
    Figure Legend Snippet: Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Techniques Used: Mutagenesis, Polymerase Chain Reaction

    Related Articles

    SYBR Green Assay:

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. Supplemental Figure S6: Titration of Hemo KlenTaq with an intact Taq polymerase in TaqMan and SYBR Green reactions improves PCR yield. .. A: PAGE analyses of end-point PCR products of reactions supplemented with indicated volume of Hemo KlenTaq in TaqMan or SYBR Green master mixes as indicated.

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. A: PAGE analyses of end-point PCR products of reactions supplemented with indicated volume of Hemo KlenTaq in TaqMan or SYBR Green master mixes as indicated. .. B: qPCR analyses of these reactions.

    Amplification:

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. Of importance, the complementing Taq polymerases produced, on average, approximately 30-fold more amplification products from plasma miR-16 than did the intact polymerase alone (see B at ), which suggests that overcoming blood-borne inhibitors of Taq polymerase using Hemo KlenTaq yields an overall increase in detection sensitivity and specificity of circulating miRNAs. .. To test whether overcoming endogenous inhibitors with complementing polymerases provides proportionally higher miRNA quantitation, we analyzed circulating miRNAs in the blood of six healthy individuals (see at ).

    Mutagenesis:

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. Hemo KlenTaq is a mutant Taq DNA polymerase that has 100-fold lower sensitivity to blood inhibitors than does wild-type Taq. .. Our analysis indicates that Hemo KlenTaq amplified miR-16, more efficiently than did Phusion ( A; see also A at ), and standard Taq DNA polymerase yielded low or no detectable PCR products in the same samples ( D).

    Isolation:

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. A: Functioning of GoTaq, Phire, Phusion, and Hemo KlenTaq was tested using cDNA of miRNA isolated from the indicated amount of starting volume of serum using end-point PCR (40 cycles). (−) indicates that PCR reaction was performed using Phire (left lane) or GoTaq (right lane) but with no template. .. B: Quantitation using TaqMan.

    Titration:

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. Supplemental Figure S6: Titration of Hemo KlenTaq with an intact Taq polymerase in TaqMan and SYBR Green reactions improves PCR yield. .. A: PAGE analyses of end-point PCR products of reactions supplemented with indicated volume of Hemo KlenTaq in TaqMan or SYBR Green master mixes as indicated.

    Produced:

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. Of importance, the complementing Taq polymerases produced, on average, approximately 30-fold more amplification products from plasma miR-16 than did the intact polymerase alone (see B at ), which suggests that overcoming blood-borne inhibitors of Taq polymerase using Hemo KlenTaq yields an overall increase in detection sensitivity and specificity of circulating miRNAs. .. To test whether overcoming endogenous inhibitors with complementing polymerases provides proportionally higher miRNA quantitation, we analyzed circulating miRNAs in the blood of six healthy individuals (see at ).

    other:

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: We tested enzymes reported to be more resistant to inhibitors: Phusion, Phire, and Hemo KlenTaq.

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: This phenomenon may be a consequence of lack of the editing and proofreading 5′- > 3′ exonuclease domain in Hemo KlenTaq.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. A: PAGE analyses of end-point PCR products of reactions supplemented with indicated volume of Hemo KlenTaq in TaqMan or SYBR Green master mixes as indicated. .. B: qPCR analyses of these reactions.

    Polymerase Chain Reaction:

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. Hemo KlenTaq (New England BioLabs, Inc., Ipswich, MA) PCR was performed in 25-μL volumes according to the manufacturer's instructions, including attempts to reduce nonspecific priming by assembling PCR reactions on ice and transfer of reactions to the thermocycler preheated to 95°C. .. PCR using cocktails containing GoTaq DNA polymerase and Hemo KlenTaq polymerase were performed using GoTaq qPCR Mastermix (Promega Corp.) for SYBR Green quantitation or TaqMan Universal PCR Mastermix (Applied Biosystems, Inc.).

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. Supplemental Figure S6: Titration of Hemo KlenTaq with an intact Taq polymerase in TaqMan and SYBR Green reactions improves PCR yield. .. A: PAGE analyses of end-point PCR products of reactions supplemented with indicated volume of Hemo KlenTaq in TaqMan or SYBR Green master mixes as indicated.

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. A: Functioning of GoTaq, Phire, Phusion, and Hemo KlenTaq was tested using cDNA of miRNA isolated from the indicated amount of starting volume of serum using end-point PCR (40 cycles). (−) indicates that PCR reaction was performed using Phire (left lane) or GoTaq (right lane) but with no template. .. B: Quantitation using TaqMan.

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation
    Article Snippet: .. A: PAGE analyses of end-point PCR products of reactions supplemented with indicated volume of Hemo KlenTaq in TaqMan or SYBR Green master mixes as indicated. .. B: qPCR analyses of these reactions.

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  • 96
    New England Biolabs hemo klentaq
    Mutant TaqDNA polymerase, <t>Hemo</t> <t>KlenTaq</t> improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star
    Hemo Klentaq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hemo klentaq/product/New England Biolabs
    Average 96 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    hemo klentaq - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    Image Search Results


    Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: Plasma Components Affect Accuracy of Circulating Cancer-Related MicroRNA Quantitation

    doi: 10.1016/j.jmoldx.2011.09.002

    Figure Lengend Snippet: Mutant TaqDNA polymerase, Hemo KlenTaq improves the sensitivity of miRNA detection. A: Hemo KlenTaq (HK) was used to detect miR-16 ( arrows ) in 200, 50, and 10 μL plasma and serum (see C). Additional PCR products are indicated by a star

    Article Snippet: Supplemental Figure S6: Titration of Hemo KlenTaq with an intact Taq polymerase in TaqMan and SYBR Green reactions improves PCR yield.

    Techniques: Mutagenesis, Polymerase Chain Reaction