bst dna polymerase  (New England Biolabs)


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    Name:
    Bst DNA Polymerase Full Length
    Description:
    Bst DNA Polymerase Full Length 500 units
    Catalog Number:
    m0328s
    Price:
    69
    Size:
    500 units
    Category:
    Thermostable DNA Polymerases
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    Structured Review

    New England Biolabs bst dna polymerase
    Bst DNA Polymerase Full Length
    Bst DNA Polymerase Full Length 500 units
    https://www.bioz.com/result/bst dna polymerase/product/New England Biolabs
    Average 90 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    bst dna polymerase - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: A Comparison of In-House Real-Time LAMP Assays with a Commercial Assay for the Detection of Pathogenic Bacteria
    Article Snippet: The experiment was performed before DNA extraction, therefore, cross pollution caused by DNA template as well as amplified products of LAMP reactions can be avoided, and the cause of false-positive results can be objectively judged. .. 1.2 mmol/L each dNTP, 6 mmol/L MgSO4 , 1 × Bst DNA polymerase buffer (New England Biolabs, Beverly, MA, USA) (20 mmol/L Tris-HCl (pH = 8.8), 10 mmol/L KCl, 10 mmol/L (NH4 )2 SO4 , 2 mmol/L MgSO4 , 0.1% TritonX-100), 8 Units of Bst DNA large fragments (New England Biolabs).

    Nucleic Acid Electrophoresis:

    Article Title: Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction
    Article Snippet: The reaction mixture contained 1x Bst DNA polymerase buffer, 5 mM MgSO4 , 400 mM betaine, 1.2 mM dNTPs, 0.8 μ M F3 and B3 primers, 2 μ M FIP and BIP primers, and 8 U Bst DNA polymerase (New England Biolabs), as well as 5 ng of each DNA extract as a template in a final volume of 25 μ l. The reaction was carried out at 65°C for 45 min and was followed by inactivation of the enzyme at 80°C for 5 min, as described by Whitfield et al. [ ]. .. After the gel electrophoresis, the gel was stained with ethidium bromide and visualized under ultraviolet light.

    Article Title: Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction
    Article Snippet: The reaction mixture contained 1x Bst DNA polymerase buffer, 5 mM MgSO4 , 400 mM betaine, 1.2 mM dNTPs, 0.8 μ M F3 and B3 primers, 2 μ M FIP and BIP primers, and 8 U Bst DNA polymerase (New England Biolabs), as well as 5 ng of each DNA extract as a template in a final volume of 25 μ l. The reaction was carried out at 65°C for 45 min and was followed by inactivation of the enzyme at 80°C for 5 min, as described by Whitfield et al. [ ]. .. After the gel electrophoresis, the gel was stained with ethidium bromide and visualized under ultraviolet light.

    Negative Control:

    Article Title: Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP assay was carried out in a total of 25 µl reaction mixture containing: 10× Bst-DNA polymerase buffer (2.5 mM each), betaine (1.6 M), deoxynucleotide triphosphates (2.5 mM each), MgSO4 (8 mM), F3 and B3 primers (0.2 µM each), FIP and BIP (1.6 µM each), loop-F and loop-B (0.8 µM each), Bst DNA polymerase (8 U) (New England Biolabs, Beverly, Massachusetts, USA), and template DNA (2 µl). .. No template DNA was added in the 'negative control' reaction.

    Amplification:

    Article Title: Rapid Detection of Mycoplasma pneumoniae by Loop-Mediated Isothermal Amplification (LAMP) in Clinical Respiratory Specimens
    Article Snippet: Amplification was performed using a thermoblock ( ). .. The LAMP reactions was performed in 25 μL total volume containing 2.5 μL Bst DNA polymerase buffer, 1.6 μM of each FIP and BIP primers, 0.2 μM of each F3 and B3 primers, 1.4 mM of each deoxynucleotide triphosphate (dNTP), 0.8 M betaine, 8 units of Bst DNA polymerase (New England BioLabs, Ipswich, MA) and 2 μL of DNA.

    Article Title: Visual Detection of High-Risk Human Papillomavirus Genotypes 16, 18, 45, 52, and 58 by Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye ▿
    Article Snippet: LAMP was performed in a one-step reaction in a 25-μl mixture containing 2.5 μl Bst DNA polymerase buffer (10×), 2.5 μl deoxynucleoside triphosphates (dNTPs) (10 mM; New England BioLabs, Ipswich, MA), 1 μl Bst DNA polymerase (8 U/μl; New England BioLabs, Ipswich, MA), 1 μl betaine (250 mM), 1 μl MgSO4 (150 mM), 1 μl HNB (3 mM; Lemongreen, Shanghai, China),1 μl of each primer (F3 and B3, 5 pmol/μl; BIP and FIP, 40 pmol/μl; and LF and LB, 20 pmol/μl), and 2 μl DNA. .. At the same time, a positive amplification was also indicated by a color change from violet to sky blue.

    Article Title: Development of coupling controlled polymerizations by adapter-ligation in mate-pair sequencing for detection of various genomic variants in one single assay
    Article Snippet: .. Of note, after purification of Ad1 PCR products, a total of 20 µg amplified DNA, which could be a mixture of each amplified DNA with equal quantity (from the six patients) or from one single sample (Trisomy 2 or 8), was used for dsCir DNA formation. naCNT was performed with 1 pmol dsCir DNA; Bst DNA Polymerase, Full Length (NEB); Klenow fragment (Enzymatics, Inc.); and limited dNTPs. ..

    Article Title: Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP primers ( ) for the specific amplification of the target DNA were designed from the EF1α gene sequences of G. lamblia using Primer Explorer V4 software ( http://primerexplorer.jp/e ). .. LAMP assay was carried out in a total of 25 µl reaction mixture containing: 10× Bst-DNA polymerase buffer (2.5 mM each), betaine (1.6 M), deoxynucleotide triphosphates (2.5 mM each), MgSO4 (8 mM), F3 and B3 primers (0.2 µM each), FIP and BIP (1.6 µM each), loop-F and loop-B (0.8 µM each), Bst DNA polymerase (8 U) (New England Biolabs, Beverly, Massachusetts, USA), and template DNA (2 µl).

    Article Title: Three Tests Used to Identify Non-Culturable Form of Helicobacter pylori in Water Samples
    Article Snippet: Paragraph title: 3.6. Optimization of Loop-Mediated Isothermal Amplification ... LAMP reaction mixture was prepared as follows: D.D.W: 5.2 µL, Betaine 5 Mol: 4 µL, dNTP (10 mM): 3.5 µL, 10X buffer: 2.5 µL, MgSo4 (100 mM): 1.8 µL, Mix Ӏ: 1 µL, Mix ӀӀ: 1 µL, Bst DNA polymerase enzyme (New England BioLabs; Lot:33/110806): 1 µL, target DNA (extracted DNA from standard strain): 5 µL, and total volume was 25 µL.

    Article Title: Loop-mediated Isothermal Amplification-Single Nucleotide Polymorphism Analysis for Detection and Differentiation of Wild-type and Vaccine Strains of Mink Enteritis Virus
    Article Snippet: LAMP-SNP reactions were performed in a total volume of 25 μL and contained 0.4 μL (10 pM) each of the F3 and B3 primers, 2.5 μL (10 pM) each of the FIP and BIP primers, 3.5 μL (10 mM) of dNTPs, 2.5 μL of 10 × Bst DNA polymerase buffer, 0.5 μL (8 U) of Bst DNA polymerase (New England BioLabs, Herts, UK), 2 μL of template DNA, 4 μL (100 mM) of MgSO4 , and 6 μL (20 μM) of betaine. .. The LAMP amplification products were analyzed by 2.0% (w/v) agarose gel electrophoresis.

    Article Title: A Comparison of In-House Real-Time LAMP Assays with a Commercial Assay for the Detection of Pathogenic Bacteria
    Article Snippet: Paragraph title: 3.2. Determination of Non-Specific Amplification ... 1.2 mmol/L each dNTP, 6 mmol/L MgSO4 , 1 × Bst DNA polymerase buffer (New England Biolabs, Beverly, MA, USA) (20 mmol/L Tris-HCl (pH = 8.8), 10 mmol/L KCl, 10 mmol/L (NH4 )2 SO4 , 2 mmol/L MgSO4 , 0.1% TritonX-100), 8 Units of Bst DNA large fragments (New England Biolabs).

    Article Title: Loop-mediated Isothermal Amplification-Single Nucleotide Polymorphism Analysis for Detection and Differentiation of Wild-type and Vaccine Strains of Mink Enteritis Virus
    Article Snippet: LAMP-SNP reactions were performed in a total volume of 25 μL and contained 0.4 μL (10 pM) each of the F3 and B3 primers, 2.5 μL (10 pM) each of the FIP and BIP primers, 3.5 μL (10 mM) of dNTPs, 2.5 μL of 10 × Bst DNA polymerase buffer, 0.5 μL (8 U) of Bst DNA polymerase (New England BioLabs, Herts, UK), 2 μL of template DNA, 4 μL (100 mM) of MgSO4 , and 6 μL (20 μM) of betaine. .. The LAMP amplification products were analyzed by 2.0% (w/v) agarose gel electrophoresis.

    Agarose Gel Electrophoresis:

    Article Title: Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP assay was carried out in a total of 25 µl reaction mixture containing: 10× Bst-DNA polymerase buffer (2.5 mM each), betaine (1.6 M), deoxynucleotide triphosphates (2.5 mM each), MgSO4 (8 mM), F3 and B3 primers (0.2 µM each), FIP and BIP (1.6 µM each), loop-F and loop-B (0.8 µM each), Bst DNA polymerase (8 U) (New England Biolabs, Beverly, Massachusetts, USA), and template DNA (2 µl). .. Also, the products (5 µl) were examined on a 2% agarose gel with DL2000 (TaKaRa, Dalian, China) to estimate the sizes of amplification products and stained with ethidium bromide.

    Article Title: Loop-mediated Isothermal Amplification-Single Nucleotide Polymorphism Analysis for Detection and Differentiation of Wild-type and Vaccine Strains of Mink Enteritis Virus
    Article Snippet: LAMP-SNP reactions were performed in a total volume of 25 μL and contained 0.4 μL (10 pM) each of the F3 and B3 primers, 2.5 μL (10 pM) each of the FIP and BIP primers, 3.5 μL (10 mM) of dNTPs, 2.5 μL of 10 × Bst DNA polymerase buffer, 0.5 μL (8 U) of Bst DNA polymerase (New England BioLabs, Herts, UK), 2 μL of template DNA, 4 μL (100 mM) of MgSO4 , and 6 μL (20 μM) of betaine. .. The LAMP amplification products were analyzed by 2.0% (w/v) agarose gel electrophoresis.

    Article Title: Loop-mediated Isothermal Amplification-Single Nucleotide Polymorphism Analysis for Detection and Differentiation of Wild-type and Vaccine Strains of Mink Enteritis Virus
    Article Snippet: LAMP-SNP reactions were performed in a total volume of 25 μL and contained 0.4 μL (10 pM) each of the F3 and B3 primers, 2.5 μL (10 pM) each of the FIP and BIP primers, 3.5 μL (10 mM) of dNTPs, 2.5 μL of 10 × Bst DNA polymerase buffer, 0.5 μL (8 U) of Bst DNA polymerase (New England BioLabs, Herts, UK), 2 μL of template DNA, 4 μL (100 mM) of MgSO4 , and 6 μL (20 μM) of betaine. .. The LAMP amplification products were analyzed by 2.0% (w/v) agarose gel electrophoresis.

    Plasmid Preparation:

    Article Title: Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye
    Article Snippet: .. LAMP reaction The preliminary LAMP for the astrovirus DNA in the plasmid template was carried out in a 25 μl reaction containing 0.2 μmol·L-1 each of F3 and B3, 1.6 μmol·L-1 each of FIP and BIP, l mmol·L-1 dNTPs, l mol·L-1 betaine, 6 mmol·L-1 MgSO4 , 2.5 μL 10× Bst-DNA Polymerase Buffer, 8 U Bst DNA polymerase (NEB, Beijing, China) and 5 μL template DNA. ..

    Synthesized:

    Article Title: A Comparison of In-House Real-Time LAMP Assays with a Commercial Assay for the Detection of Pathogenic Bacteria
    Article Snippet: Determination of Non-Specific Amplification After the LAMP primers were synthesized by Sangon Biotech Co., Ltd (Shanghai, China), the non-specific amplification of the 12 in-house real-time LAMP assays as well as the commercial Isothermal Master Mix kit were determined via the corresponding negative controls with no genomic DNA. .. 1.2 mmol/L each dNTP, 6 mmol/L MgSO4 , 1 × Bst DNA polymerase buffer (New England Biolabs, Beverly, MA, USA) (20 mmol/L Tris-HCl (pH = 8.8), 10 mmol/L KCl, 10 mmol/L (NH4 )2 SO4 , 2 mmol/L MgSO4 , 0.1% TritonX-100), 8 Units of Bst DNA large fragments (New England Biolabs).

    Sequencing:

    Article Title: Development of coupling controlled polymerizations by adapter-ligation in mate-pair sequencing for detection of various genomic variants in one single assay
    Article Snippet: Of note, after purification of Ad1 PCR products, a total of 20 µg amplified DNA, which could be a mixture of each amplified DNA with equal quantity (from the six patients) or from one single sample (Trisomy 2 or 8), was used for dsCir DNA formation. naCNT was performed with 1 pmol dsCir DNA; Bst DNA Polymerase, Full Length (NEB); Klenow fragment (Enzymatics, Inc.); and limited dNTPs. .. Primer extension was accomplished using ttCPE as described above, and the reaction mixture was incubated at 92°C for 5 min, 56°C for 60 s and 60°C for 40 s; The sample was then purified with Agencourt AmpureXP beads. ttCPE products were ligated to the 5′-end of Ad2 and amplified with Pfu Turbo Cx ( ). ssCirs and DNBs were prepared for sequencing on the BGISEQ-500 platform (BGI-Wuhan, Wuhan, China).

    Labeling:

    Article Title: Genotyping three SNPs affecting warfarin drug response by isothermal real-time HAD assays
    Article Snippet: Each 50 µL reaction contained 3.5 mM MgSO4 , 30 mM NaCl, 400 µM of each dTTP, dCTP, and dGTP, 3.4 mM dATP, 200 to 300 ng of the UvrD DNA helicase from Thermoanaerobacter tengcongensis (BioHelix), 50 ng of the Single Stranded DNA binding protein (SSB) from Sulfolobus solfataricus (BioHelix), and 40 to 80 units of the full-length DNA polymerase from Bacillus stearothermophilus (Bst) (New England Biolabs, Ipswich, MA). .. The probes were from Applied Biosystems (Carlsbad, CA) for minor groove binding domain (MGB) labeled probes or Integrated DNA Technologies for locked-nucleic acid (LNA) labeled probes.

    Article Title: Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction
    Article Snippet: The inner primer FIP was labeled with a biotin group at the 5′ end. .. The reaction mixture contained 1x Bst DNA polymerase buffer, 5 mM MgSO4 , 400 mM betaine, 1.2 mM dNTPs, 0.8 μ M F3 and B3 primers, 2 μ M FIP and BIP primers, and 8 U Bst DNA polymerase (New England Biolabs) as well as 5 ng of each DNA extract as a template in a final volume of 25 μ l. The reaction was carried out at 65°C for 45 min and was followed by inactivation of the enzyme at 80°C for 5 min.

    Article Title: Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction
    Article Snippet: The inner primer FIP was labeled with a biotin group at the 5′ end. .. The reaction mixture contained 1x Bst DNA polymerase buffer, 5 mM MgSO4 , 400 mM betaine, 1.2 mM dNTPs, 0.8 μ M F3 and B3 primers, 2 μ M FIP and BIP primers, and 8 U Bst DNA polymerase (New England Biolabs) as well as 5 ng of each DNA extract as a template in a final volume of 25 μ l. The reaction was carried out at 65°C for 45 min and was followed by inactivation of the enzyme at 80°C for 5 min.

    Purification:

    Article Title: Boosting functionality of synthetic DNA circuits with tailored deactivation
    Article Snippet: For the excitable network, Bst DNA polymerase, full length (NEB) was used instead at a concentration of 40 U ml−1 (0.8% of the stock solution). .. The thermophilic 5′– > 3′ exonuclease from Thermus thermophilus ttRecJ was purified in the laboratory as previously described .

    Article Title: Development of coupling controlled polymerizations by adapter-ligation in mate-pair sequencing for detection of various genomic variants in one single assay
    Article Snippet: .. Of note, after purification of Ad1 PCR products, a total of 20 µg amplified DNA, which could be a mixture of each amplified DNA with equal quantity (from the six patients) or from one single sample (Trisomy 2 or 8), was used for dsCir DNA formation. naCNT was performed with 1 pmol dsCir DNA; Bst DNA Polymerase, Full Length (NEB); Klenow fragment (Enzymatics, Inc.); and limited dNTPs. ..

    Article Title: Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification
    Article Snippet: Genomic DNA (gDNA) was extracted directly from purified cysts using a QIAamp DNA Stool Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer's instructions. .. LAMP assay was carried out in a total of 25 µl reaction mixture containing: 10× Bst-DNA polymerase buffer (2.5 mM each), betaine (1.6 M), deoxynucleotide triphosphates (2.5 mM each), MgSO4 (8 mM), F3 and B3 primers (0.2 µM each), FIP and BIP (1.6 µM each), loop-F and loop-B (0.8 µM each), Bst DNA polymerase (8 U) (New England Biolabs, Beverly, Massachusetts, USA), and template DNA (2 µl).

    SYBR Green Assay:

    Article Title: Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP assay was carried out in a total of 25 µl reaction mixture containing: 10× Bst-DNA polymerase buffer (2.5 mM each), betaine (1.6 M), deoxynucleotide triphosphates (2.5 mM each), MgSO4 (8 mM), F3 and B3 primers (0.2 µM each), FIP and BIP (1.6 µM each), loop-F and loop-B (0.8 µM each), Bst DNA polymerase (8 U) (New England Biolabs, Beverly, Massachusetts, USA), and template DNA (2 µl). .. The LAMP products were visually detected further by adding 1 µl of 1:10 diluted 10,000× concentration of SYBR Green I (Invitrogen, Carlsbad, California, USA) to the reaction tube.

    Article Title: Loop-mediated Isothermal Amplification-Single Nucleotide Polymorphism Analysis for Detection and Differentiation of Wild-type and Vaccine Strains of Mink Enteritis Virus
    Article Snippet: LAMP-SNP reactions were performed in a total volume of 25 μL and contained 0.4 μL (10 pM) each of the F3 and B3 primers, 2.5 μL (10 pM) each of the FIP and BIP primers, 3.5 μL (10 mM) of dNTPs, 2.5 μL of 10 × Bst DNA polymerase buffer, 0.5 μL (8 U) of Bst DNA polymerase (New England BioLabs, Herts, UK), 2 μL of template DNA, 4 μL (100 mM) of MgSO4 , and 6 μL (20 μM) of betaine. .. Besides agarose gel electrophoresis, the products of LAMP-SNP were judged by naked eyes with the addition of SYBR Green I (Solarbio, Beijing, China) dye into the tubes.

    Article Title: Loop-mediated Isothermal Amplification-Single Nucleotide Polymorphism Analysis for Detection and Differentiation of Wild-type and Vaccine Strains of Mink Enteritis Virus
    Article Snippet: LAMP-SNP reactions were performed in a total volume of 25 μL and contained 0.4 μL (10 pM) each of the F3 and B3 primers, 2.5 μL (10 pM) each of the FIP and BIP primers, 3.5 μL (10 mM) of dNTPs, 2.5 μL of 10 × Bst DNA polymerase buffer, 0.5 μL (8 U) of Bst DNA polymerase (New England BioLabs, Herts, UK), 2 μL of template DNA, 4 μL (100 mM) of MgSO4 , and 6 μL (20 μM) of betaine. .. Besides agarose gel electrophoresis, the products of LAMP-SNP were judged by naked eyes with the addition of SYBR Green I (Solarbio, Beijing, China) dye into the tubes.

    Polymerase Chain Reaction:

    Article Title: Development of coupling controlled polymerizations by adapter-ligation in mate-pair sequencing for detection of various genomic variants in one single assay
    Article Snippet: .. Of note, after purification of Ad1 PCR products, a total of 20 µg amplified DNA, which could be a mixture of each amplified DNA with equal quantity (from the six patients) or from one single sample (Trisomy 2 or 8), was used for dsCir DNA formation. naCNT was performed with 1 pmol dsCir DNA; Bst DNA Polymerase, Full Length (NEB); Klenow fragment (Enzymatics, Inc.); and limited dNTPs. ..

    Article Title: Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification
    Article Snippet: A 208 bp fragment of the EF1α gene was amplificated using PCR with the outer primers B3 and F3, and the specificity of the outer primers was confirmed by BLAST search ( http://www.ncbi.nlm.nih.gov/Blast ) in the NCBI database. .. LAMP assay was carried out in a total of 25 µl reaction mixture containing: 10× Bst-DNA polymerase buffer (2.5 mM each), betaine (1.6 M), deoxynucleotide triphosphates (2.5 mM each), MgSO4 (8 mM), F3 and B3 primers (0.2 µM each), FIP and BIP (1.6 µM each), loop-F and loop-B (0.8 µM each), Bst DNA polymerase (8 U) (New England Biolabs, Beverly, Massachusetts, USA), and template DNA (2 µl).

    Incubation:

    Article Title: Rapid Detection of Mycoplasma pneumoniae by Loop-Mediated Isothermal Amplification (LAMP) in Clinical Respiratory Specimens
    Article Snippet: The LAMP reactions was performed in 25 μL total volume containing 2.5 μL Bst DNA polymerase buffer, 1.6 μM of each FIP and BIP primers, 0.2 μM of each F3 and B3 primers, 1.4 mM of each deoxynucleotide triphosphate (dNTP), 0.8 M betaine, 8 units of Bst DNA polymerase (New England BioLabs, Ipswich, MA) and 2 μL of DNA. .. The mixture was incubated at 62 °C for 60 min in a thermoblock (Dena gene Tajhiz, Iran)

    Article Title: Visual Detection of High-Risk Human Papillomavirus Genotypes 16, 18, 45, 52, and 58 by Loop-Mediated Isothermal Amplification with Hydroxynaphthol Blue Dye ▿
    Article Snippet: LAMP was performed in a one-step reaction in a 25-μl mixture containing 2.5 μl Bst DNA polymerase buffer (10×), 2.5 μl deoxynucleoside triphosphates (dNTPs) (10 mM; New England BioLabs, Ipswich, MA), 1 μl Bst DNA polymerase (8 U/μl; New England BioLabs, Ipswich, MA), 1 μl betaine (250 mM), 1 μl MgSO4 (150 mM), 1 μl HNB (3 mM; Lemongreen, Shanghai, China),1 μl of each primer (F3 and B3, 5 pmol/μl; BIP and FIP, 40 pmol/μl; and LF and LB, 20 pmol/μl), and 2 μl DNA. .. The mixtures were incubated at 63°C for 65 min. A Loopamp real-time LA-320 turbidimeter (Eiken Chemical Co., Ltd., Tokyo, Japan) was used to monitor the accumulation of magnesium pyrophosphate spectrophotometrically at 650 nm.

    Article Title: Development of coupling controlled polymerizations by adapter-ligation in mate-pair sequencing for detection of various genomic variants in one single assay
    Article Snippet: Of note, after purification of Ad1 PCR products, a total of 20 µg amplified DNA, which could be a mixture of each amplified DNA with equal quantity (from the six patients) or from one single sample (Trisomy 2 or 8), was used for dsCir DNA formation. naCNT was performed with 1 pmol dsCir DNA; Bst DNA Polymerase, Full Length (NEB); Klenow fragment (Enzymatics, Inc.); and limited dNTPs. .. Primer extension was accomplished using ttCPE as described above, and the reaction mixture was incubated at 92°C for 5 min, 56°C for 60 s and 60°C for 40 s; The sample was then purified with Agencourt AmpureXP beads. ttCPE products were ligated to the 5′-end of Ad2 and amplified with Pfu Turbo Cx ( ). ssCirs and DNBs were prepared for sequencing on the BGISEQ-500 platform (BGI-Wuhan, Wuhan, China).

    Lamp Assay:

    Article Title: Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification
    Article Snippet: .. LAMP assay was carried out in a total of 25 µl reaction mixture containing: 10× Bst-DNA polymerase buffer (2.5 mM each), betaine (1.6 M), deoxynucleotide triphosphates (2.5 mM each), MgSO4 (8 mM), F3 and B3 primers (0.2 µM each), FIP and BIP (1.6 µM each), loop-F and loop-B (0.8 µM each), Bst DNA polymerase (8 U) (New England Biolabs, Beverly, Massachusetts, USA), and template DNA (2 µl). .. No template DNA was added in the 'negative control' reaction.

    Article Title: Loop-mediated Isothermal Amplification-Single Nucleotide Polymorphism Analysis for Detection and Differentiation of Wild-type and Vaccine Strains of Mink Enteritis Virus
    Article Snippet: Detection and differentiation of wild-type and vaccine MEV strains by LAMP-SNP assay Both wild-type and vaccine viruses were detected by MEV-VP2 -LAMP assay . .. LAMP-SNP reactions were performed in a total volume of 25 μL and contained 0.4 μL (10 pM) each of the F3 and B3 primers, 2.5 μL (10 pM) each of the FIP and BIP primers, 3.5 μL (10 mM) of dNTPs, 2.5 μL of 10 × Bst DNA polymerase buffer, 0.5 μL (8 U) of Bst DNA polymerase (New England BioLabs, Herts, UK), 2 μL of template DNA, 4 μL (100 mM) of MgSO4 , and 6 μL (20 μM) of betaine.

    Article Title: Loop-mediated Isothermal Amplification-Single Nucleotide Polymorphism Analysis for Detection and Differentiation of Wild-type and Vaccine Strains of Mink Enteritis Virus
    Article Snippet: Both wild-type and vaccine viruses were detected by MEV- VP2 -LAMP assay . .. LAMP-SNP reactions were performed in a total volume of 25 μL and contained 0.4 μL (10 pM) each of the F3 and B3 primers, 2.5 μL (10 pM) each of the FIP and BIP primers, 3.5 μL (10 mM) of dNTPs, 2.5 μL of 10 × Bst DNA polymerase buffer, 0.5 μL (8 U) of Bst DNA polymerase (New England BioLabs, Herts, UK), 2 μL of template DNA, 4 μL (100 mM) of MgSO4 , and 6 μL (20 μM) of betaine.

    Modification:

    Article Title: A Comparison of In-House Real-Time LAMP Assays with a Commercial Assay for the Detection of Pathogenic Bacteria
    Article Snippet: 1.2 mmol/L each dNTP, 6 mmol/L MgSO4 , 1 × Bst DNA polymerase buffer (New England Biolabs, Beverly, MA, USA) (20 mmol/L Tris-HCl (pH = 8.8), 10 mmol/L KCl, 10 mmol/L (NH4 )2 SO4 , 2 mmol/L MgSO4 , 0.1% TritonX-100), 8 Units of Bst DNA large fragments (New England Biolabs). .. The LAMP assays were modified by adding 1 × EvaGreen and 1 × Rox, the experiment only on negative controls was carried out on StepOne™ System (Applied Biosystems, Foster City, CA, USA) at the described temperature (65 °C for the inv A of Salmonella , rfbE of Escherichia coli O157 , wzy of Escherichia coli O26, wzy of Escherichia coli O45, wzx of Escherichia coli O103, wzy of Escherichia coli O111, wzy of Escherichia coli O121, wzx of Escherichia coli O145, wzy of Escherichia coli O157; 64 °C for nuc of Staphylococcus aureus ; 63 °C for sob A of Streptococcus agalactiae ) for 50 min, and each experiment was repeated three times.

    Staining:

    Article Title: Rapid and sensitive detection of human astrovirus in water samples by loop-mediated isothermal amplification with hydroxynaphthol blue dye
    Article Snippet: LAMP reaction The preliminary LAMP for the astrovirus DNA in the plasmid template was carried out in a 25 μl reaction containing 0.2 μmol·L-1 each of F3 and B3, 1.6 μmol·L-1 each of FIP and BIP, l mmol·L-1 dNTPs, l mol·L-1 betaine, 6 mmol·L-1 MgSO4 , 2.5 μL 10× Bst-DNA Polymerase Buffer, 8 U Bst DNA polymerase (NEB, Beijing, China) and 5 μL template DNA. .. The LAMP products (5 μL) were electrophoresed on 1.5% agarose gels and stained with GoldView to determine the optimal conditions.

    Article Title: Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction
    Article Snippet: The reaction mixture contained 1x Bst DNA polymerase buffer, 5 mM MgSO4 , 400 mM betaine, 1.2 mM dNTPs, 0.8 μ M F3 and B3 primers, 2 μ M FIP and BIP primers, and 8 U Bst DNA polymerase (New England Biolabs), as well as 5 ng of each DNA extract as a template in a final volume of 25 μ l. The reaction was carried out at 65°C for 45 min and was followed by inactivation of the enzyme at 80°C for 5 min, as described by Whitfield et al. [ ]. .. After the gel electrophoresis, the gel was stained with ethidium bromide and visualized under ultraviolet light.

    Article Title: Time Course of Detection of Human Male DNA from Stained Blood Sample on Various Surfaces by Loop Mediated Isothermal Amplification and Polymerase Chain Reaction
    Article Snippet: The reaction mixture contained 1x Bst DNA polymerase buffer, 5 mM MgSO4 , 400 mM betaine, 1.2 mM dNTPs, 0.8 μ M F3 and B3 primers, 2 μ M FIP and BIP primers, and 8 U Bst DNA polymerase (New England Biolabs), as well as 5 ng of each DNA extract as a template in a final volume of 25 μ l. The reaction was carried out at 65°C for 45 min and was followed by inactivation of the enzyme at 80°C for 5 min, as described by Whitfield et al. [ ]. .. After the gel electrophoresis, the gel was stained with ethidium bromide and visualized under ultraviolet light.

    Article Title: Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP assay was carried out in a total of 25 µl reaction mixture containing: 10× Bst-DNA polymerase buffer (2.5 mM each), betaine (1.6 M), deoxynucleotide triphosphates (2.5 mM each), MgSO4 (8 mM), F3 and B3 primers (0.2 µM each), FIP and BIP (1.6 µM each), loop-F and loop-B (0.8 µM each), Bst DNA polymerase (8 U) (New England Biolabs, Beverly, Massachusetts, USA), and template DNA (2 µl). .. Also, the products (5 µl) were examined on a 2% agarose gel with DL2000 (TaKaRa, Dalian, China) to estimate the sizes of amplification products and stained with ethidium bromide.

    Binding Assay:

    Article Title: Genotyping three SNPs affecting warfarin drug response by isothermal real-time HAD assays
    Article Snippet: .. Each 50 µL reaction contained 3.5 mM MgSO4 , 30 mM NaCl, 400 µM of each dTTP, dCTP, and dGTP, 3.4 mM dATP, 200 to 300 ng of the UvrD DNA helicase from Thermoanaerobacter tengcongensis (BioHelix), 50 ng of the Single Stranded DNA binding protein (SSB) from Sulfolobus solfataricus (BioHelix), and 40 to 80 units of the full-length DNA polymerase from Bacillus stearothermophilus (Bst) (New England Biolabs, Ipswich, MA). .. The primers were obtained from Operon (Huntsville, AL) or Integrated DNA Technologies (Coralville, IA).

    Helicase-dependent Amplification:

    Article Title: Genotyping three SNPs affecting warfarin drug response by isothermal real-time HAD assays
    Article Snippet: Paragraph title: 2.5. Real-time isothermal HDA assays ... Each 50 µL reaction contained 3.5 mM MgSO4 , 30 mM NaCl, 400 µM of each dTTP, dCTP, and dGTP, 3.4 mM dATP, 200 to 300 ng of the UvrD DNA helicase from Thermoanaerobacter tengcongensis (BioHelix), 50 ng of the Single Stranded DNA binding protein (SSB) from Sulfolobus solfataricus (BioHelix), and 40 to 80 units of the full-length DNA polymerase from Bacillus stearothermophilus (Bst) (New England Biolabs, Ipswich, MA).

    IA:

    Article Title: Genotyping three SNPs affecting warfarin drug response by isothermal real-time HAD assays
    Article Snippet: Each 50 µL reaction contained 3.5 mM MgSO4 , 30 mM NaCl, 400 µM of each dTTP, dCTP, and dGTP, 3.4 mM dATP, 200 to 300 ng of the UvrD DNA helicase from Thermoanaerobacter tengcongensis (BioHelix), 50 ng of the Single Stranded DNA binding protein (SSB) from Sulfolobus solfataricus (BioHelix), and 40 to 80 units of the full-length DNA polymerase from Bacillus stearothermophilus (Bst) (New England Biolabs, Ipswich, MA). .. The primers were obtained from Operon (Huntsville, AL) or Integrated DNA Technologies (Coralville, IA).

    Concentration Assay:

    Article Title: Boosting functionality of synthetic DNA circuits with tailored deactivation
    Article Snippet: .. For the excitable network, Bst DNA polymerase, full length (NEB) was used instead at a concentration of 40 U ml−1 (0.8% of the stock solution). .. The thermophilic 5′– > 3′ exonuclease from Thermus thermophilus ttRecJ was purified in the laboratory as previously described .

    Article Title: Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP assay was carried out in a total of 25 µl reaction mixture containing: 10× Bst-DNA polymerase buffer (2.5 mM each), betaine (1.6 M), deoxynucleotide triphosphates (2.5 mM each), MgSO4 (8 mM), F3 and B3 primers (0.2 µM each), FIP and BIP (1.6 µM each), loop-F and loop-B (0.8 µM each), Bst DNA polymerase (8 U) (New England Biolabs, Beverly, Massachusetts, USA), and template DNA (2 µl). .. The LAMP products were visually detected further by adding 1 µl of 1:10 diluted 10,000× concentration of SYBR Green I (Invitrogen, Carlsbad, California, USA) to the reaction tube.

    Article Title: Three Tests Used to Identify Non-Culturable Form of Helicobacter pylori in Water Samples
    Article Snippet: LAMP reaction mixture was prepared as follows: D.D.W: 5.2 µL, Betaine 5 Mol: 4 µL, dNTP (10 mM): 3.5 µL, 10X buffer: 2.5 µL, MgSo4 (100 mM): 1.8 µL, Mix Ӏ: 1 µL, Mix ӀӀ: 1 µL, Bst DNA polymerase enzyme (New England BioLabs; Lot:33/110806): 1 µL, target DNA (extracted DNA from standard strain): 5 µL, and total volume was 25 µL. .. In Mix Ӏ the concentration of FIP and BIP primers were 40, 10 µL D.D.W in 100 µL total volume respectively, and in Mix ӀӀ the concentration of LF, and LB were 20 and 60 µL D.D.W in 100 µL total volume, respectively.

    Software:

    Article Title: Sensitive and Rapid Detection of Giardia lamblia Infection in Pet Dogs using Loop-Mediated Isothermal Amplification
    Article Snippet: LAMP primers ( ) for the specific amplification of the target DNA were designed from the EF1α gene sequences of G. lamblia using Primer Explorer V4 software ( http://primerexplorer.jp/e ). .. LAMP assay was carried out in a total of 25 µl reaction mixture containing: 10× Bst-DNA polymerase buffer (2.5 mM each), betaine (1.6 M), deoxynucleotide triphosphates (2.5 mM each), MgSO4 (8 mM), F3 and B3 primers (0.2 µM each), FIP and BIP (1.6 µM each), loop-F and loop-B (0.8 µM each), Bst DNA polymerase (8 U) (New England Biolabs, Beverly, Massachusetts, USA), and template DNA (2 µl).

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  • 90
    New England Biolabs bst dna polymerase
    Bst Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bst dna polymerase/product/New England Biolabs
    Average 90 stars, based on 55 article reviews
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    bst dna polymerase - by Bioz Stars, 2020-01
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