t7 dna ligase  (New England Biolabs)


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    Name:
    T7 DNA Ligase
    Description:
    T7 DNA Ligase 750 000 units
    Catalog Number:
    m0318l
    Price:
    276
    Category:
    DNA Ligases
    Size:
    750 000 units
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    New England Biolabs t7 dna ligase
    T7 DNA Ligase
    T7 DNA Ligase 750 000 units
    https://www.bioz.com/result/t7 dna ligase/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t7 dna ligase - by Bioz Stars, 2021-03
    95/100 stars

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    Related Articles

    Ligation:

    Article Title: Improved transposon-based library preparation for the Ion Torrent platform
    Article Snippet: This pentaplet is complementary to the 5′-end of the exposed overhang and ensures ligation of the fragment to the oligonucleotide. .. The ligation reaction is carried out by T7 DNA ligase (New England BioLabs) to avoid blunt end ligation. .. The resulting library lacks complementary regions capable of forming hairpins; moreover, whereas only homodupexes have DNA strands equipped with both adaptors and are suitable for sequencing, heteroduplexes lack the second adaptor and do not participate in clonal amplifcation ( ).

    Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers
    Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using T7 DNA ligase (NEB #M0318). .. Control ‘empty’ lentiCRISPR_v2 lacking expression of any sgRNAs was generated by BsmBI digestion, followed by blunting of ends (NEB #E1201) and ligation with T4 DNA ligase (NEB #M0202).

    Expressing:

    Article Title: Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation
    Article Snippet: For generating landing pad vectors from expression vectors without the correct overhangs, an oligonucleotide stuffer was used to complete the overhangs. .. TA-splitHalo BFP plasmids were made in 10 μL reactions comprising 20 fmol Kanamycin ColE1 digested backbone, 40 fmol TA-splitHalo fusion expression vectors, 40 fmol PGK-mTagBFP2 expression vector, 10x T4 Ligase Buffer (NEB B0202S), Esp31 (NEB R0734S/L), and T7 DNA Ligase (NEB M0318S/L) with the same cycling conditions as the part vectors. .. Transfection of HeLa cells in 8-well chamber flasks for TA-splitHalo Architecture BenchmarkingFor transfections, HeLa cells were seeded in an 8-well chamber flask at 20k cells per well in 225 μL DMEM +1% Penicillin/Streptomycin (P/S) 10% Fetal Bovine Serum (FBS).

    Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers
    Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using T7 DNA ligase (NEB #M0318). .. Control ‘empty’ lentiCRISPR_v2 lacking expression of any sgRNAs was generated by BsmBI digestion, followed by blunting of ends (NEB #E1201) and ligation with T4 DNA ligase (NEB #M0202).

    Plasmid Preparation:

    Article Title: Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation
    Article Snippet: For generating landing pad vectors from expression vectors without the correct overhangs, an oligonucleotide stuffer was used to complete the overhangs. .. TA-splitHalo BFP plasmids were made in 10 μL reactions comprising 20 fmol Kanamycin ColE1 digested backbone, 40 fmol TA-splitHalo fusion expression vectors, 40 fmol PGK-mTagBFP2 expression vector, 10x T4 Ligase Buffer (NEB B0202S), Esp31 (NEB R0734S/L), and T7 DNA Ligase (NEB M0318S/L) with the same cycling conditions as the part vectors. .. Transfection of HeLa cells in 8-well chamber flasks for TA-splitHalo Architecture BenchmarkingFor transfections, HeLa cells were seeded in an 8-well chamber flask at 20k cells per well in 225 μL DMEM +1% Penicillin/Streptomycin (P/S) 10% Fetal Bovine Serum (FBS).

    Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers
    Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using T7 DNA ligase (NEB #M0318). .. Control ‘empty’ lentiCRISPR_v2 lacking expression of any sgRNAs was generated by BsmBI digestion, followed by blunting of ends (NEB #E1201) and ligation with T4 DNA ligase (NEB #M0202).

    Clone Assay:

    Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers
    Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using T7 DNA ligase (NEB #M0318). .. Control ‘empty’ lentiCRISPR_v2 lacking expression of any sgRNAs was generated by BsmBI digestion, followed by blunting of ends (NEB #E1201) and ligation with T4 DNA ligase (NEB #M0202).

    Generated:

    Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers
    Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using T7 DNA ligase (NEB #M0318). .. Control ‘empty’ lentiCRISPR_v2 lacking expression of any sgRNAs was generated by BsmBI digestion, followed by blunting of ends (NEB #E1201) and ligation with T4 DNA ligase (NEB #M0202).

    Polymerase Chain Reaction:

    Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers
    Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using T7 DNA ligase (NEB #M0318). .. Control ‘empty’ lentiCRISPR_v2 lacking expression of any sgRNAs was generated by BsmBI digestion, followed by blunting of ends (NEB #E1201) and ligation with T4 DNA ligase (NEB #M0202).

    Amplification:

    Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers
    Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using T7 DNA ligase (NEB #M0318). .. Control ‘empty’ lentiCRISPR_v2 lacking expression of any sgRNAs was generated by BsmBI digestion, followed by blunting of ends (NEB #E1201) and ligation with T4 DNA ligase (NEB #M0202).

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  • About
  • News
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  • Team
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  • Contact
  • Bioz Stars
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  • 95
    New England Biolabs t7 dna ligase
    T7 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t7 dna ligase/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    t7 dna ligase - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

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