t7 dna ligase (New England Biolabs)
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Name:
T7 DNA Ligase
Description:
T7 DNA Ligase 750 000 units
Catalog Number:
M0318L
Price:
276
Category:
DNA Ligases
Size:
750 000 units
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Structured Review
New England Biolabs
t7 dna ligase
T7 DNA Ligase 750 000 units
https://www.bioz.com/result/t7 dna ligase/product/New England Biolabs
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
T7 DNA Ligase 750 000 units
https://www.bioz.com/result/t7 dna ligase/product/New England Biolabs
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t7 dna ligase - by Bioz Stars,
2021-05
99/100 stars
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Ligation:Article Title: Improved transposon-based library preparation for the Ion Torrent platform Article Snippet: This pentaplet is complementary to the 5′-end of the exposed overhang and ensures ligation of the fragment to the oligonucleotide. .. The ligation reaction is carried out by Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using CRISPR:Article Title: Hsp70 and NF-kB Mediated Control of Innate Inflammatory Responses in a Canine Macrophage Cell Line Article Snippet: Briefly, 5′ ends of gRNA oligos (10 μM) were phosphorylated and annealed with T4 PNK (New England Biolabs, Ipswich, UK) using the following cycle parameters: 30 min at 37 °C, 5 min at 95 °C, then ramp down to 25 °C at 0.3 °C/min and 4 °C. .. Subsequently, the annealed gRNAs were ligated into the CRISPR-concatemer vector using 100 ng CRISPR-concatemer vector, 10.0 μL oligo mixture, 1.0 μL BSA-containing restriction enzyme buffer (10×), 1.0 μL DTT (10 mM), 1.0 μL ATP (10 mM), 1.0 μL Bbs I, 1.0 μL Plasmid Preparation:Article Title: Hsp70 and NF-kB Mediated Control of Innate Inflammatory Responses in a Canine Macrophage Cell Line Article Snippet: Briefly, 5′ ends of gRNA oligos (10 μM) were phosphorylated and annealed with T4 PNK (New England Biolabs, Ipswich, UK) using the following cycle parameters: 30 min at 37 °C, 5 min at 95 °C, then ramp down to 25 °C at 0.3 °C/min and 4 °C. .. Subsequently, the annealed gRNAs were ligated into the CRISPR-concatemer vector using 100 ng CRISPR-concatemer vector, 10.0 μL oligo mixture, 1.0 μL BSA-containing restriction enzyme buffer (10×), 1.0 μL DTT (10 mM), 1.0 μL ATP (10 mM), 1.0 μL Bbs I, 1.0 μL Article Title: Biological Parts for Kluyveromyces marxianus Synthetic Biology Article Snippet: .. In a typical reaction, 40 fmol of each insert (either as an existing level I plasmid or PCR product) were combined with 20 fmol of the plasmid containing the backbone for the final product to be assembled along with 1μL T4 DNA ligase 0.5 μL each of Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Article Title: Rapid characterization of spike variants via mammalian cell surface display Article Snippet: Golden Gate compatible parts were arranged in a 384-well Echo Source Plate (PP-0200) and transferred to 96-well PCR destination plates using the Echo 525. .. Each well of the 96-well destination plate received the following Golden Gate reaction mixture: 0.5 μL of Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly Article Snippet: The PCR products were purified and then digested for 2 hrs at 37°C with Aar I (Thermo Fisher Scientific). .. A Level 1 Acceptor Vector A was also digested with AarI and ligated to the Auxiliary Plasmid cassettes with 1 μl Polymerase Chain Reaction:Article Title: Biological Parts for Kluyveromyces marxianus Synthetic Biology Article Snippet: .. In a typical reaction, 40 fmol of each insert (either as an existing level I plasmid or PCR product) were combined with 20 fmol of the plasmid containing the backbone for the final product to be assembled along with 1μL T4 DNA ligase 0.5 μL each of Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Clone Assay:Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Expressing:Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Generated:Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Amplification:Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Construct:Article Title: Fronto-temporal dementia risk gene TMEM106B has opposing effects in different lysosomal storage disorders Article Snippet: .. To generate sgRNA-expressing constructs, pX552 was digested using SapI Fast Digest (ThermoFisher Scientific, D1934) and annealed oligos were ligated using |