t7 dna ligase (New England Biolabs)
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Name:
T7 DNA Ligase
Description:
T7 DNA Ligase 750 000 units
Catalog Number:
m0318l
Price:
276
Category:
DNA Ligases
Size:
750 000 units
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Structured Review
New England Biolabs
t7 dna ligase
T7 DNA Ligase 750 000 units
https://www.bioz.com/result/t7 dna ligase/product/New England Biolabs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
T7 DNA Ligase 750 000 units
https://www.bioz.com/result/t7 dna ligase/product/New England Biolabs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
t7 dna ligase - by Bioz Stars,
2021-03
95/100 stars
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Related Articles
Ligation:Article Title: Improved transposon-based library preparation for the Ion Torrent platform Article Snippet: This pentaplet is complementary to the 5′-end of the exposed overhang and ensures ligation of the fragment to the oligonucleotide. .. The ligation reaction is carried out by Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Expressing:Article Title: Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation Article Snippet: For generating landing pad vectors from expression vectors without the correct overhangs, an oligonucleotide stuffer was used to complete the overhangs. .. TA-splitHalo BFP plasmids were made in 10 μL reactions comprising 20 fmol Kanamycin ColE1 digested backbone, 40 fmol TA-splitHalo fusion expression vectors, 40 fmol PGK-mTagBFP2 expression vector, 10x T4 Ligase Buffer (NEB B0202S), Esp31 (NEB R0734S/L), and Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Plasmid Preparation:Article Title: Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation Article Snippet: For generating landing pad vectors from expression vectors without the correct overhangs, an oligonucleotide stuffer was used to complete the overhangs. .. TA-splitHalo BFP plasmids were made in 10 μL reactions comprising 20 fmol Kanamycin ColE1 digested backbone, 40 fmol TA-splitHalo fusion expression vectors, 40 fmol PGK-mTagBFP2 expression vector, 10x T4 Ligase Buffer (NEB B0202S), Esp31 (NEB R0734S/L), and Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Clone Assay:Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Generated:Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Polymerase Chain Reaction:Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using Amplification:Article Title: Identification of focally amplified lineage-specific super-enhancers in human epithelial cancers Article Snippet: Cells stably expressing KRAB-dCas9 were then subsequently infected with sgRNAs and selected with 2μg/ml puromycin. .. For deletion of the e3 enhancer, tandem U6-promoter-sgRNA and H1-promoter-sgRNA cassettes were cloned into lentiCRISPR_v2 (Addgene #60954) for single vector expression of two sgRNAs as follows: 1) U6-sgRNA and H1-sgRNA products were generated by PCR amplification using the primers listed in , 2) PCR products were then digested with BsmBI to generate compatible sticky ends, 3) finally, three-way ligation of the two PCR products and BsmBI-digested lentiCRISPR_v2 was performed using |