nb btsi  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    Nb BtsI
    Description:
    Nb BtsI 1 000 units
    Catalog Number:
    r0707l
    Price:
    70
    Size:
    1 000 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs nb btsi
    Nb BtsI
    Nb BtsI 1 000 units
    https://www.bioz.com/result/nb btsi/product/New England Biolabs
    Average 95 stars, based on 625 article reviews
    Price from $9.99 to $1999.99
    nb btsi - by Bioz Stars, 2020-08
    95/100 stars

    Images

    1) Product Images from "Genome-wide mapping of embedded ribonucleotides and other non-canonical nucleotides using emRiboSeq and EndoSeq"

    Article Title: Genome-wide mapping of embedded ribonucleotides and other non-canonical nucleotides using emRiboSeq and EndoSeq

    Journal: Nature protocols

    doi: 10.1038/nprot.2015.099

    Library quality control and anticipated results. ( a ) Sonicated DNA separated by agarose gel electrophoresis (Step 25) shows an average fragment size of approximately 400 bp. ( b ) Bioanalyzer result (Step 84) for an emRiboSeq library shows a typical trace (left) and gel-like image (right) with a peak for fragments between ˜180 and ˜300 bp in size (black bar). Standards (green and purple bars) of defined size and amount allow quantification. FU, arbitrary fluorescence units. ( c ) Agarose gel electrophoresis of PCR products after 15, 16 and 17 cycles of amplification (Steps 81-83) of the same library shows product between 200 and 300 bp in size. ( d ) Sequencing results for libraries generated using Nb.BtsI are highly reproducibility between different strains (POL, wildtype polymerase; pol1-L868M, increased Pol-α ribonucleotide incorporation) after normalizing read counts to sequence tags per million (TPM). The majority of bona fide Nb.BtsI sites were present at maximal frequency, although some sites were present at lower frequencies. This is the result of partial loss during size selection because of their close proximity to other cleavage sites, a highly reproducible finding between independent libraries (Spearman's rho=0.82, p
    Figure Legend Snippet: Library quality control and anticipated results. ( a ) Sonicated DNA separated by agarose gel electrophoresis (Step 25) shows an average fragment size of approximately 400 bp. ( b ) Bioanalyzer result (Step 84) for an emRiboSeq library shows a typical trace (left) and gel-like image (right) with a peak for fragments between ˜180 and ˜300 bp in size (black bar). Standards (green and purple bars) of defined size and amount allow quantification. FU, arbitrary fluorescence units. ( c ) Agarose gel electrophoresis of PCR products after 15, 16 and 17 cycles of amplification (Steps 81-83) of the same library shows product between 200 and 300 bp in size. ( d ) Sequencing results for libraries generated using Nb.BtsI are highly reproducibility between different strains (POL, wildtype polymerase; pol1-L868M, increased Pol-α ribonucleotide incorporation) after normalizing read counts to sequence tags per million (TPM). The majority of bona fide Nb.BtsI sites were present at maximal frequency, although some sites were present at lower frequencies. This is the result of partial loss during size selection because of their close proximity to other cleavage sites, a highly reproducible finding between independent libraries (Spearman's rho=0.82, p

    Techniques Used: Sonication, Agarose Gel Electrophoresis, Fluorescence, Polymerase Chain Reaction, Amplification, Sequencing, Generated, Selection

    2) Product Images from "Genome-wide mapping of embedded ribonucleotides and other non-canonical nucleotides using emRiboSeq and EndoSeq"

    Article Title: Genome-wide mapping of embedded ribonucleotides and other non-canonical nucleotides using emRiboSeq and EndoSeq

    Journal: Nature protocols

    doi: 10.1038/nprot.2015.099

    Library quality control and anticipated results. ( a ) Sonicated DNA separated by agarose gel electrophoresis (Step 25) shows an average fragment size of approximately 400 bp. ( b ) Bioanalyzer result (Step 84) for an emRiboSeq library shows a typical trace (left) and gel-like image (right) with a peak for fragments between ˜180 and ˜300 bp in size (black bar). Standards (green and purple bars) of defined size and amount allow quantification. FU, arbitrary fluorescence units. ( c ) Agarose gel electrophoresis of PCR products after 15, 16 and 17 cycles of amplification (Steps 81-83) of the same library shows product between 200 and 300 bp in size. ( d ) Sequencing results for libraries generated using Nb.BtsI are highly reproducibility between different strains (POL, wildtype polymerase; pol1-L868M, increased Pol-α ribonucleotide incorporation) after normalizing read counts to sequence tags per million (TPM). The majority of bona fide Nb.BtsI sites were present at maximal frequency, although some sites were present at lower frequencies. This is the result of partial loss during size selection because of their close proximity to other cleavage sites, a highly reproducible finding between independent libraries (Spearman's rho=0.82, p
    Figure Legend Snippet: Library quality control and anticipated results. ( a ) Sonicated DNA separated by agarose gel electrophoresis (Step 25) shows an average fragment size of approximately 400 bp. ( b ) Bioanalyzer result (Step 84) for an emRiboSeq library shows a typical trace (left) and gel-like image (right) with a peak for fragments between ˜180 and ˜300 bp in size (black bar). Standards (green and purple bars) of defined size and amount allow quantification. FU, arbitrary fluorescence units. ( c ) Agarose gel electrophoresis of PCR products after 15, 16 and 17 cycles of amplification (Steps 81-83) of the same library shows product between 200 and 300 bp in size. ( d ) Sequencing results for libraries generated using Nb.BtsI are highly reproducibility between different strains (POL, wildtype polymerase; pol1-L868M, increased Pol-α ribonucleotide incorporation) after normalizing read counts to sequence tags per million (TPM). The majority of bona fide Nb.BtsI sites were present at maximal frequency, although some sites were present at lower frequencies. This is the result of partial loss during size selection because of their close proximity to other cleavage sites, a highly reproducible finding between independent libraries (Spearman's rho=0.82, p

    Techniques Used: Sonication, Agarose Gel Electrophoresis, Fluorescence, Polymerase Chain Reaction, Amplification, Sequencing, Generated, Selection

    Related Articles

    Amplification:

    Article Title: Single-particle analysis of full-length human poly(ADP-ribose) polymerase 1
    Article Snippet: .. A part of the dsDNA was digested for 4 h at 37°C with Nb.BtsI nicking endonuclease (New England Biolabs), which recognized 5′-GCAGTG(nick)-3′ sequence and introduced single-strand break, to produce a 105 bp nicked-dsDNA in the middle of the amplified sequence ( ). .. Single-particle electron microscopy The purified h-PARP1 (5 μM) and 105 bp dsDNA (0.18 μM) or nicked-dsDNA (0.18 μM) were mixed and diluted 100 times with the equilibrium buffer for 3-AB-Sepharose column chromatography.

    Magnetic Beads:

    Article Title: Methods for the Preparation of Large Quantities of Complex Single-Stranded Oligonucleotide Libraries
    Article Snippet: .. Sequential removal of PCR primer ends with on-bead BspQI digestion Post hybridization, the 3′-end PCR primer containing BtsI recognition site is digested with 50 U Nb.BtsI at 37°C for 2 h (total reaction volume 100 µl; 1x NEB, 1x BSA) followed by capture of cDNA on streptavidin coated magnetic beads. .. 50 µl of beads (250 pmol binding capacity) is used to immobilize 5′-biotinylated cDNA.

    Polymerase Chain Reaction:

    Article Title: Methods for the Preparation of Large Quantities of Complex Single-Stranded Oligonucleotide Libraries
    Article Snippet: .. Sequential removal of PCR primer ends with on-bead BspQI digestion Post hybridization, the 3′-end PCR primer containing BtsI recognition site is digested with 50 U Nb.BtsI at 37°C for 2 h (total reaction volume 100 µl; 1x NEB, 1x BSA) followed by capture of cDNA on streptavidin coated magnetic beads. .. 50 µl of beads (250 pmol binding capacity) is used to immobilize 5′-biotinylated cDNA.

    Article Title: Comparison of DNA decatenation by Escherichia coli topoisomerase IV and topoisomerase III: implications for non-equilibrium topology simplification
    Article Snippet: .. The 4-kb multi-nicked DNA molecules were prepared by PCR of pFC94 ( ) from position 5422–9520 with a biotin-labelled forward primer and digoxigenin-labelled reverse primer (Eurofins MWG Operon) and were nicked with four nicking enzymes: Nb.BsmI, Nt.AIwI, Nb.BtsI and Nt.BtsNBI (New England Biolabs), generating 14 nicks located over the middle third of the DNA. ..

    Generated:

    Article Title: Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression
    Article Snippet: .. The repair mPlum plasmid (mPlum.Hx) was engineered by placing the AAG-specific base lesion hypoxanthine (Hx) lesion into the open reading frame of mPlum . mPlum.Hx Substrate Containing a Site-Specific Hypoxanthine (Hx) was generated as follows; single-stranded DNA was obtained by nicking the plasmid pmax:mPlum with Nb.BtsI (NEB R0707S) for 4 h at 37 °C in NEB Buffer 4 (NEB B7004). .. After phenol chloroform extraction the DNA was incubated with 5 U of ExoIII (NEB M0206S) per µg of DNA at 37 °C for 1 h in NEB buffer 1 (NEB B7001) to digest the nicked strand.

    Sequencing:

    Article Title: Single-particle analysis of full-length human poly(ADP-ribose) polymerase 1
    Article Snippet: .. A part of the dsDNA was digested for 4 h at 37°C with Nb.BtsI nicking endonuclease (New England Biolabs), which recognized 5′-GCAGTG(nick)-3′ sequence and introduced single-strand break, to produce a 105 bp nicked-dsDNA in the middle of the amplified sequence ( ). .. Single-particle electron microscopy The purified h-PARP1 (5 μM) and 105 bp dsDNA (0.18 μM) or nicked-dsDNA (0.18 μM) were mixed and diluted 100 times with the equilibrium buffer for 3-AB-Sepharose column chromatography.

    Recombinant:

    Article Title: Genome-wide mapping of embedded ribonucleotides and other non-canonical nucleotides using emRiboSeq and EndoSeq
    Article Snippet: .. CRITICAL: alternative sources of recombinant type 2 RNase H enzymes may be used 10% (wt/vol) Bovine Serum Albumin Fraction V (BSA, Roche, cat. no. 10 735 086 001) Magnesium chloride (MgCl2 , Sigma, cat. no. M2670) Nb.BtsI, Supplied with 10x CutSmart® buffer (New England Biolabs, cat. no. R0707) BciVI, Supplied with 10x CutSmart® buffer (New England Biolabs, cat. no. R0596) Shrimp Alkaline Phosphatase (SAP), supplied with 10x reaction buffer (Affymetrix USB® , cat. no. 70092Z) Dynabeads® M-280 Streptavidin (Life Technologies, cat. no. 11205D) Tri-sodium citrate (Sigma, cat. no. C8532) Glycogen (Roche, cat. no. 10 901 393 001) Sodium acetate (NaOAc, Sigma, cat. no. S2889) Sodium hydroxide (NaOH, Sigma, cat. no. 38215) CAUTION Sodium hydroxide is corrosive. ..

    Plasmid Preparation:

    Article Title: Alkyladenine DNA glycosylase associates with transcription elongation to coordinate DNA repair with gene expression
    Article Snippet: .. The repair mPlum plasmid (mPlum.Hx) was engineered by placing the AAG-specific base lesion hypoxanthine (Hx) lesion into the open reading frame of mPlum . mPlum.Hx Substrate Containing a Site-Specific Hypoxanthine (Hx) was generated as follows; single-stranded DNA was obtained by nicking the plasmid pmax:mPlum with Nb.BtsI (NEB R0707S) for 4 h at 37 °C in NEB Buffer 4 (NEB B7004). .. After phenol chloroform extraction the DNA was incubated with 5 U of ExoIII (NEB M0206S) per µg of DNA at 37 °C for 1 h in NEB buffer 1 (NEB B7001) to digest the nicked strand.

    Hybridization:

    Article Title: Methods for the Preparation of Large Quantities of Complex Single-Stranded Oligonucleotide Libraries
    Article Snippet: .. Sequential removal of PCR primer ends with on-bead BspQI digestion Post hybridization, the 3′-end PCR primer containing BtsI recognition site is digested with 50 U Nb.BtsI at 37°C for 2 h (total reaction volume 100 µl; 1x NEB, 1x BSA) followed by capture of cDNA on streptavidin coated magnetic beads. .. 50 µl of beads (250 pmol binding capacity) is used to immobilize 5′-biotinylated cDNA.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs terminal transferase
    Terminal Transferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/terminal transferase/product/New England Biolabs
    Average 99 stars, based on 63 article reviews
    Price from $9.99 to $1999.99
    terminal transferase - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results