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New England Biolabs tdt
Tdt, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tdt/product/New England Biolabs
Average 95 stars, based on 44 article reviews
Price from $9.99 to $1999.99
tdt - by Bioz Stars, 2020-01
95/100 stars

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Ligation:

Article Title:
Article Snippet: Agarose (electrophoresis grade, Sigma, cat. no. A9539) SYBR® Gold nucleic acid stain, 10,000x in DMSO (Life Technologies, cat. No. S-11494) 100 bp DNA ladder (e.g. Promega, cat. no. G210A, supplied with Blue/Orange 6X Loading Dye) NEBNext® end repair module (New England Biolabs, cat. no. E6050) NEBNext® dA-tailing module (New England Biolabs, cat. no. E6053) NEBNext® Quick Ligation module (New England Biolabs, cat. no. E6056) Custom oligonucleotides; ours were synthesised by Eurogentec and purified using manufacturer recommended methods ( ). .. Terminal Transferase, supplied with 10x reaction buffer and 2.5 mM CoCl2 (New England Biolabs, cat. no. M0315) 10 mM ddATP, Sequencing Grade Na-salt pH 8.3 (Roche, cat. no. 03 732 738 001) RNase HII (New England Biolabs, cat. no. M0288) or recombinant human RNase H2 (purified as previously described ) for emRiboSeq; other recombinant (nicking) endonuclease of interest for EndoSeq.

Purification:

Article Title:
Article Snippet: .. Terminal Transferase, supplied with 10x reaction buffer and 2.5 mM CoCl2 (New England Biolabs, cat. no. M0315) 10 mM ddATP, Sequencing Grade Na-salt pH 8.3 (Roche, cat. no. 03 732 738 001) RNase HII (New England Biolabs, cat. no. M0288) or recombinant human RNase H2 (purified as previously described ) for emRiboSeq; other recombinant (nicking) endonuclease of interest for EndoSeq. .. CRITICAL: alternative sources of recombinant type 2 RNase H enzymes may be used 10% (wt/vol) Bovine Serum Albumin Fraction V (BSA, Roche, cat. no. 10 735 086 001) Magnesium chloride (MgCl2 , Sigma, cat. no. M2670) Nb.BtsI, Supplied with 10x CutSmart® buffer (New England Biolabs, cat. no. R0707) BciVI, Supplied with 10x CutSmart® buffer (New England Biolabs, cat. no. R0596) Shrimp Alkaline Phosphatase (SAP), supplied with 10x reaction buffer (Affymetrix USB® , cat. no. 70092Z) Dynabeads® M-280 Streptavidin (Life Technologies, cat. no. 11205D) Tri-sodium citrate (Sigma, cat. no. C8532) Glycogen (Roche, cat. no. 10 901 393 001) Sodium acetate (NaOAc, Sigma, cat. no. S2889) Sodium hydroxide (NaOH, Sigma, cat. no. 38215) CAUTION Sodium hydroxide is corrosive.

Electrophoresis:

Article Title:
Article Snippet: Agarose (electrophoresis grade, Sigma, cat. no. A9539) SYBR® Gold nucleic acid stain, 10,000x in DMSO (Life Technologies, cat. No. S-11494) 100 bp DNA ladder (e.g. Promega, cat. no. G210A, supplied with Blue/Orange 6X Loading Dye) NEBNext® end repair module (New England Biolabs, cat. no. E6050) NEBNext® dA-tailing module (New England Biolabs, cat. no. E6053) NEBNext® Quick Ligation module (New England Biolabs, cat. no. E6056) Custom oligonucleotides; ours were synthesised by Eurogentec and purified using manufacturer recommended methods ( ). .. Terminal Transferase, supplied with 10x reaction buffer and 2.5 mM CoCl2 (New England Biolabs, cat. no. M0315) 10 mM ddATP, Sequencing Grade Na-salt pH 8.3 (Roche, cat. no. 03 732 738 001) RNase HII (New England Biolabs, cat. no. M0288) or recombinant human RNase H2 (purified as previously described ) for emRiboSeq; other recombinant (nicking) endonuclease of interest for EndoSeq.

Sequencing:

Article Title:
Article Snippet: .. Terminal Transferase, supplied with 10x reaction buffer and 2.5 mM CoCl2 (New England Biolabs, cat. no. M0315) 10 mM ddATP, Sequencing Grade Na-salt pH 8.3 (Roche, cat. no. 03 732 738 001) RNase HII (New England Biolabs, cat. no. M0288) or recombinant human RNase H2 (purified as previously described ) for emRiboSeq; other recombinant (nicking) endonuclease of interest for EndoSeq. .. CRITICAL: alternative sources of recombinant type 2 RNase H enzymes may be used 10% (wt/vol) Bovine Serum Albumin Fraction V (BSA, Roche, cat. no. 10 735 086 001) Magnesium chloride (MgCl2 , Sigma, cat. no. M2670) Nb.BtsI, Supplied with 10x CutSmart® buffer (New England Biolabs, cat. no. R0707) BciVI, Supplied with 10x CutSmart® buffer (New England Biolabs, cat. no. R0596) Shrimp Alkaline Phosphatase (SAP), supplied with 10x reaction buffer (Affymetrix USB® , cat. no. 70092Z) Dynabeads® M-280 Streptavidin (Life Technologies, cat. no. 11205D) Tri-sodium citrate (Sigma, cat. no. C8532) Glycogen (Roche, cat. no. 10 901 393 001) Sodium acetate (NaOAc, Sigma, cat. no. S2889) Sodium hydroxide (NaOH, Sigma, cat. no. 38215) CAUTION Sodium hydroxide is corrosive.

other:

Article Title:
Article Snippet: After antigen retrieval and proteolytic digestion by citrate buffer and proteinase K, each section was incubated with 50 units of TdT (M0315; New England Biolabs, Ipswich, MA, USA) and 20 nmol bromodeoxyuridine at 37°C for 90 min. After the TUNEL reaction, the labelled cells were probed with the mouse monoclonal antibody against bromodeoxyuridine followed by the biotinylated horse anti-mouse IgG and then detected using the Vectastain ABC alkaline phosphatase kit and its chromogen substrate Vector Red.

Polymerase Chain Reaction:

Article Title:
Article Snippet: Terminal Transferase, supplied with 10x reaction buffer and 2.5 mM CoCl2 (New England Biolabs, cat. no. M0315) 10 mM ddATP, Sequencing Grade Na-salt pH 8.3 (Roche, cat. no. 03 732 738 001) RNase HII (New England Biolabs, cat. no. M0288) or recombinant human RNase H2 (purified as previously described ) for emRiboSeq; other recombinant (nicking) endonuclease of interest for EndoSeq. .. Phusion Flash High-Fidelity PCR Master Mix (Thermo Scientific, cat. no. F-548) E-Gel® SizeSelect™ Agarose Gels, 2% (Life Technologies, cat. no. G6610-02) Agilent High Sensitivity DNA Kit (Agilent Technologies, cat. no. 5067-4626) Potassium Chloride (KCl, Sigma, cat. no. P9541) Ion 318™ Chip Kit v2 (Life Technologies, cat. no. 4484534) or Ion PI™ Chip Kit v2 (Life Technologies, cat. no. 4482413) Ion PGM™ Template OT2 200 Kit (Life Technologies, cat. no. 4480974) or Ion PI™ Template OT2 200 Kit v2 (Life Technologies, cat. no. 4485146) Ion PGM™ Sequencing 200 Kit v2 (Life Technologies, cat. no. 4482006) or Ion PI™ sequencing kit (Life Technologies, cat. no. 4485149).

Staining:

Article Title:
Article Snippet: Agarose (electrophoresis grade, Sigma, cat. no. A9539) SYBR® Gold nucleic acid stain, 10,000x in DMSO (Life Technologies, cat. No. S-11494) 100 bp DNA ladder (e.g. Promega, cat. no. G210A, supplied with Blue/Orange 6X Loading Dye) NEBNext® end repair module (New England Biolabs, cat. no. E6050) NEBNext® dA-tailing module (New England Biolabs, cat. no. E6053) NEBNext® Quick Ligation module (New England Biolabs, cat. no. E6056) Custom oligonucleotides; ours were synthesised by Eurogentec and purified using manufacturer recommended methods ( ). .. Terminal Transferase, supplied with 10x reaction buffer and 2.5 mM CoCl2 (New England Biolabs, cat. no. M0315) 10 mM ddATP, Sequencing Grade Na-salt pH 8.3 (Roche, cat. no. 03 732 738 001) RNase HII (New England Biolabs, cat. no. M0288) or recombinant human RNase H2 (purified as previously described ) for emRiboSeq; other recombinant (nicking) endonuclease of interest for EndoSeq.

Recombinant:

Article Title:
Article Snippet: .. Terminal Transferase, supplied with 10x reaction buffer and 2.5 mM CoCl2 (New England Biolabs, cat. no. M0315) 10 mM ddATP, Sequencing Grade Na-salt pH 8.3 (Roche, cat. no. 03 732 738 001) RNase HII (New England Biolabs, cat. no. M0288) or recombinant human RNase H2 (purified as previously described ) for emRiboSeq; other recombinant (nicking) endonuclease of interest for EndoSeq. .. CRITICAL: alternative sources of recombinant type 2 RNase H enzymes may be used 10% (wt/vol) Bovine Serum Albumin Fraction V (BSA, Roche, cat. no. 10 735 086 001) Magnesium chloride (MgCl2 , Sigma, cat. no. M2670) Nb.BtsI, Supplied with 10x CutSmart® buffer (New England Biolabs, cat. no. R0707) BciVI, Supplied with 10x CutSmart® buffer (New England Biolabs, cat. no. R0596) Shrimp Alkaline Phosphatase (SAP), supplied with 10x reaction buffer (Affymetrix USB® , cat. no. 70092Z) Dynabeads® M-280 Streptavidin (Life Technologies, cat. no. 11205D) Tri-sodium citrate (Sigma, cat. no. C8532) Glycogen (Roche, cat. no. 10 901 393 001) Sodium acetate (NaOAc, Sigma, cat. no. S2889) Sodium hydroxide (NaOH, Sigma, cat. no. 38215) CAUTION Sodium hydroxide is corrosive.

Chromatin Immunoprecipitation:

Article Title:
Article Snippet: Terminal Transferase, supplied with 10x reaction buffer and 2.5 mM CoCl2 (New England Biolabs, cat. no. M0315) 10 mM ddATP, Sequencing Grade Na-salt pH 8.3 (Roche, cat. no. 03 732 738 001) RNase HII (New England Biolabs, cat. no. M0288) or recombinant human RNase H2 (purified as previously described ) for emRiboSeq; other recombinant (nicking) endonuclease of interest for EndoSeq. .. Phusion Flash High-Fidelity PCR Master Mix (Thermo Scientific, cat. no. F-548) E-Gel® SizeSelect™ Agarose Gels, 2% (Life Technologies, cat. no. G6610-02) Agilent High Sensitivity DNA Kit (Agilent Technologies, cat. no. 5067-4626) Potassium Chloride (KCl, Sigma, cat. no. P9541) Ion 318™ Chip Kit v2 (Life Technologies, cat. no. 4484534) or Ion PI™ Chip Kit v2 (Life Technologies, cat. no. 4482413) Ion PGM™ Template OT2 200 Kit (Life Technologies, cat. no. 4480974) or Ion PI™ Template OT2 200 Kit v2 (Life Technologies, cat. no. 4485146) Ion PGM™ Sequencing 200 Kit v2 (Life Technologies, cat. no. 4482006) or Ion PI™ sequencing kit (Life Technologies, cat. no. 4485149).

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  • 90
    New England Biolabs terminal transferase
    Terminal Transferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/terminal transferase/product/New England Biolabs
    Average 90 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    terminal transferase - by Bioz Stars, 2020-01
    90/100 stars
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