precr  (New England Biolabs)


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  • 95
    Name:
    PreCR Repair Mix
    Description:
    PreCR Repair Mix 150 rxns
    Catalog Number:
    m0309l
    Price:
    641
    Size:
    150 rxns
    Category:
    DNA Template Preparation for PCR
    Buy from Supplier


    Structured Review

    New England Biolabs precr
    PreCR Repair Mix
    PreCR Repair Mix 150 rxns
    https://www.bioz.com/result/precr/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    precr - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)
    Article Snippet: Paragraph title: Pacific Biosciences sequencing of fosmid clones ... DNA was eluted from the beads by addition of 20 μl of TE buffer and incubation for 2 min in a ThermoMixer C at 1400 rpm 2 μl of PreCR Repair Mix (New England Biolabs) and 5 μl of NEBNext End Repair Reaction Buffer (New England Biolabs) in a 50 μl of total volume reaction was added to repair the damaged DNA template.

    Amplification:

    Article Title: PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations
    Article Snippet: To ameliorate PCR amplification issues caused by compromised sample quality (e.g. nicks, abasic sites etc.), a DNA repair procedure using commercial kits (e.g . .. PreCR Repair Mix by New England Biolabs) could be incorporated into the protocol.

    Construct:

    Article Title: Large Enriched Fragment Targeted Sequencing (LEFT-SEQ) Applied to Capture of Wolbachia Genomes
    Article Snippet: .. DNA repair and large insert library preparation Large insert libraries were constructed using an adaptation of NEBNext® UltraTM II DNA Library Prep protocol (New England Biolabs, US) with preliminary steps of single strand DNA elimination using Exonuclease VII treatment and DNA damage repair using PreCR® repair mix (New England Biolabs, US) (Fig. ). .. A 55 μL reaction volume contained the fragmented DNA (~500 ng), 6 μL of NEBNext® UltraTM II End Prep Reaction buffer, 1 μL of NAD+, and 1 μL of Exonuclease VII and was incubated 15 minutes at 37 °C.

    Real-time Polymerase Chain Reaction:

    Article Title: Genetic Variability of Microcystin Biosynthesis Genes in Planktothrix as Elucidated from Samples Preserved by Heat Desiccation during Three Decades
    Article Snippet: .. Evaluation of DNA treatment to reverse DNA modifications Four randomly selected DNA samples were treated with the PreCR Repair Mix and the DNA template concentration as estimated by qPCR was compared quantitatively to untreated aliquots for four different loci (16Sr DNA, Dhb, Mdha, and Hty). ..

    Incubation:

    Article Title: Whole-genome single-cell copy number profiling from formalin-fixed paraffin-embedded samples
    Article Snippet: .. Fifty nanograms of DNA were subjected to template restoration using PreCR Repair Mix (NEB) in a reaction containing 1× ThermoPol Buffer, 100 μM dNTPs, 1× NAD+ , 1 μl of PreCR mix and H2 O to 50 μl, incubated 20 min at 37°C. .. The restored DNA was concentrated using the DNA Clean & Concentrator (Zymo Research) and identical amounts of repaired and unrepaired bulk DNA were subjected to Multiplex-PCR as previously described with minor modifications.

    Article Title: Large Enriched Fragment Targeted Sequencing (LEFT-SEQ) Applied to Capture of Wolbachia Genomes
    Article Snippet: DNA repair and large insert library preparation Large insert libraries were constructed using an adaptation of NEBNext® UltraTM II DNA Library Prep protocol (New England Biolabs, US) with preliminary steps of single strand DNA elimination using Exonuclease VII treatment and DNA damage repair using PreCR® repair mix (New England Biolabs, US) (Fig. ). .. A 55 μL reaction volume contained the fragmented DNA (~500 ng), 6 μL of NEBNext® UltraTM II End Prep Reaction buffer, 1 μL of NAD+, and 1 μL of Exonuclease VII and was incubated 15 minutes at 37 °C.

    Article Title: Rapid antibiotic-resistance predictions from genome sequence data for Staphylococcus aureus and Mycobacterium tuberculosis
    Article Snippet: Without undergoing any shearing process, 2 μg of DNA were treated with PreCR Repair Mix (New England BioLabs, NEB) , to repair possible damage to the DNA that could interfere with the sequencing process. .. Following Oxford Nanopore recommendation for the optional PreCR treatment, each μg of DNA was first diluted in nuclease-free water to a volume of 85 μl to which 10 μl ThermoPol Reaction Buffer, 1 μl NAD+ , 1 μl 10 μM dNTPs and 2 μl PreCR Repair Mix were added, and the two reactions were incubated at 37 °C for 30 min.

    Article Title: Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers
    Article Snippet: The fragments were repaired with PreCR Repair Mix (New England BioLabs, Frankfurt, Germany) and stored in 10 mM PBS buffer (137 mM NaCl + 2.7 mM KCl (pH 7.4) at 25°C) at 4°C. .. For that, the magnetic beads (5 mg/ml) were gently mixed with DNA fragments (60 pM) into the MT buffer and incubated for 15 min. Before every experiment we verified the structural integrity of each probed DNA-molecule (nick-free) and its torsionally constrained immobilization, and we acquired a reference hat curve by MT overwinding experiments.

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: Nick repair was performed by using PreCR Repair Mix (New England Biolabs). .. Lambda exonuclease- and RecJf exonuclease-treated chromatin was eluted from the beads and overnight incubation at 65°C reversed the protein-DNA cross-link.

    Article Title: CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells
    Article Snippet: IP-enriched chromatin was eluted with 100 mM NaHCO3 and 1% SDS at 65°C for 2 h. To reverse cross-linking, NaCl was added to the final 200 mM concentration and incubated at 67°C for additional 4 h followed by RNase A (150 μg/ml) treatment for 1 h and then proteins were digested with proteinase K (100 μg/ml) for 3 h. The DNA was purified by phenol extraction and ethanol precipitated. .. The DNA was repaired using PreCR Repair mix (New England Biolabs, Ipswich, MA, USA) following manufacturer’s instructions.

    Article Title: Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)
    Article Snippet: .. DNA was eluted from the beads by addition of 20 μl of TE buffer and incubation for 2 min in a ThermoMixer C at 1400 rpm 2 μl of PreCR Repair Mix (New England Biolabs) and 5 μl of NEBNext End Repair Reaction Buffer (New England Biolabs) in a 50 μl of total volume reaction was added to repair the damaged DNA template. .. The reaction was incubated at 37 °C for 20 min. DNA was end-repaired by addition of 2.5 μl of NEBNext End repair mix (New England Biolabs) (incubation at 25 °C for 5 min) and then purified with 0.45× volume of AMPure PB magnetic beads and eluted with 20 μl of TE.

    HAT Assay:

    Article Title: Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers
    Article Snippet: The fragments were repaired with PreCR Repair Mix (New England BioLabs, Frankfurt, Germany) and stored in 10 mM PBS buffer (137 mM NaCl + 2.7 mM KCl (pH 7.4) at 25°C) at 4°C. .. For that, the magnetic beads (5 mg/ml) were gently mixed with DNA fragments (60 pM) into the MT buffer and incubated for 15 min. Before every experiment we verified the structural integrity of each probed DNA-molecule (nick-free) and its torsionally constrained immobilization, and we acquired a reference hat curve by MT overwinding experiments.

    Western Blot:

    Article Title: CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells
    Article Snippet: Paragraph title: Antibodies used for chromatin immunoprecipitation, western blot, and immunofluorescence ... The DNA was repaired using PreCR Repair mix (New England Biolabs, Ipswich, MA, USA) following manufacturer’s instructions.

    Flow Cytometry:

    Article Title: Robust long-read native DNA sequencing using the ONT CsgG Nanopore system
    Article Snippet: Damage to DNA was then repaired using the preCR repair mix (NEB)**, according to manufacturer recommendations and DNA subsequently purified using AMPureXP beads (Beckman Coulter). .. Another round of AMPure XP purification was then performed before the DNA library was eluted and loaded onto a running buffer-primed flow cell for sequencing.

    Article Title: Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers
    Article Snippet: The fragments were repaired with PreCR Repair Mix (New England BioLabs, Frankfurt, Germany) and stored in 10 mM PBS buffer (137 mM NaCl + 2.7 mM KCl (pH 7.4) at 25°C) at 4°C. .. The DNA fragments were attached via several antidigoxigenins to the surface of a glass cover slip of a custom-made flow cell.

    Ligation:

    Article Title: Robust long-read native DNA sequencing using the ONT CsgG Nanopore system
    Article Snippet: Damage to DNA was then repaired using the preCR repair mix (NEB)**, according to manufacturer recommendations and DNA subsequently purified using AMPureXP beads (Beckman Coulter). .. Ligation of ‘E7’ motor protein-complexed AMX adapter (ONT, NSK007)*** to genomic DNA ends was next carried out using the NEBNext Ultra II Ligation Module (NEB)****.

    Article Title: PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations
    Article Snippet: PreCR Repair Mix by New England Biolabs) could be incorporated into the protocol. .. Conversely, new library preparation techniques such as transposon-mediated tagmentation could also be used to reduce input DNA amount and increase ligation efficiency for better PCR performance [ ].

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: Briefly, the sheared DNA of chromatin-beads was repaired by the NEBNext End Repair Module (New England Biolabs) followed by the addition of a single dA overhang and ligation of the first adaptor (5′-phosphorylated) using dA-Tailing Module (New England Biolabs) and NEBNext Quick Ligation Module (New England Biolabs), respectively. .. Nick repair was performed by using PreCR Repair Mix (New England Biolabs).

    Article Title: Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)
    Article Snippet: DNA was eluted from the beads by addition of 20 μl of TE buffer and incubation for 2 min in a ThermoMixer C at 1400 rpm 2 μl of PreCR Repair Mix (New England Biolabs) and 5 μl of NEBNext End Repair Reaction Buffer (New England Biolabs) in a 50 μl of total volume reaction was added to repair the damaged DNA template. .. The ligation reaction was incubated at 25 °C for 15 min. Ligase was removed using the Monarch PCR and DNA cleanup kit (New England Biolabs).

    other:

    Article Title: Transposon Mutagenesis of the Extremely Thermophilic Bacterium Thermus thermophilus HB27
    Article Snippet: To this end we drop-dialyzed the transposon and initiated DNA repair using the PreCR Repair MIX which includes DNA polymerase and ligase activities.

    Article Title: Genetic Variability of Microcystin Biosynthesis Genes in Planktothrix as Elucidated from Samples Preserved by Heat Desiccation during Three Decades
    Article Snippet: The PreCR Repair Mix contains different enzymes for the repair of the most common lesions in DNA (abasic sites, nicks, thymidine dimers, blocked 3’ ends, oxidized guanine, oxidized pyrimidines, deaminated cytosine).

    DNA Sequencing:

    Article Title: Rapid antibiotic-resistance predictions from genome sequence data for Staphylococcus aureus and Mycobacterium tuberculosis
    Article Snippet: Nanopore sequencing The DNA library was prepared using the Genomic DNA Sequencing Kit SQK-MAP005 according the manufacturer's protocol (Version MN005_1115_revC_26Nov2014), with small modifications. .. Without undergoing any shearing process, 2 μg of DNA were treated with PreCR Repair Mix (New England BioLabs, NEB) , to repair possible damage to the DNA that could interfere with the sequencing process.

    Polymerase Chain Reaction:

    Article Title: Whole-genome single-cell copy number profiling from formalin-fixed paraffin-embedded samples
    Article Snippet: Fifty nanograms of DNA were subjected to template restoration using PreCR Repair Mix (NEB) in a reaction containing 1× ThermoPol Buffer, 100 μM dNTPs, 1× NAD+ , 1 μl of PreCR mix and H2 O to 50 μl, incubated 20 min at 37°C. .. The PCR reaction was performed with four primer sets that produce 100, 200, 300 and 400 bp fragments from non-overlapping target sites in the GAPDH gene (chr12) in 25 μl with final concentrations of 0.133 μM of each of the eight primers ( ) and 1× AmpliTaq® Gold 360 DNA Master Mix.

    Article Title: Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus
    Article Snippet: .. Linear DNase and Restriction Endonuclease Treatment of exDNA Samples and End-Point PCR of DNA Targets Prior to linear DNase and/or treatment with restriction endonuclease, exDNA samples were treated with PreCR Repair Mix (NEB) to repair nicked DNA. .. PreCR-treated samples were then either (1) treated with the ϕSa4ms-specific restriction endonucleases Psh AI and Psp XI (NEB) following manufacturer’s protocol, (2) treated with Plasmid-Safe-ATP-Dependent DNase (Epicentre), (3) treated with Psh AI and Psp XI, then the Plasmid-Safe-ATP-Dependent DNase, or (4) left solely PreCR-treated.

    Article Title: PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations
    Article Snippet: To ameliorate PCR amplification issues caused by compromised sample quality (e.g. nicks, abasic sites etc.), a DNA repair procedure using commercial kits (e.g . .. PreCR Repair Mix by New England Biolabs) could be incorporated into the protocol.

    Article Title: Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)
    Article Snippet: DNA was eluted from the beads by addition of 20 μl of TE buffer and incubation for 2 min in a ThermoMixer C at 1400 rpm 2 μl of PreCR Repair Mix (New England Biolabs) and 5 μl of NEBNext End Repair Reaction Buffer (New England Biolabs) in a 50 μl of total volume reaction was added to repair the damaged DNA template. .. The ligation reaction was incubated at 25 °C for 15 min. Ligase was removed using the Monarch PCR and DNA cleanup kit (New England Biolabs).

    Binding Assay:

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: In brief, to identify each candidate TF binding maps in vivo , the DNA bound to each candidate TF from formaldehyde cross-linked E. coli cells were isolated by chromatin immunoprecipitation (ChIP) with the specific antibodies that specifically recognize myc tag (9E10, Santa Cruz Biotechnology), and Dynabeads Pan Mouse IgG magnetic beads (Invitrogen) followed by stringent washings as described previously ( ). .. Nick repair was performed by using PreCR Repair Mix (New England Biolabs).

    Immunofluorescence:

    Article Title: CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells
    Article Snippet: Paragraph title: Antibodies used for chromatin immunoprecipitation, western blot, and immunofluorescence ... The DNA was repaired using PreCR Repair mix (New England Biolabs, Ipswich, MA, USA) following manufacturer’s instructions.

    ChIP-sequencing:

    Article Title: CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells
    Article Snippet: Chromatin IP for CHIP-Seq was performed similarly as above, except for the following modifications: MNase concentration was 0.6U/ml, digestion time was 10 min for cancer cells and 8 min for normal HcoEpiC cells, and nuclei were treated with 0.05 to 0.1% formaldehyde for gentle in situ crosslinking within intact nuclei for 30 min at RT, as indicated in ENCODE protocols, before extraction of chromatin in low salt buffer. .. The DNA was repaired using PreCR Repair mix (New England Biolabs, Ipswich, MA, USA) following manufacturer’s instructions.

    DNA Extraction:

    Article Title: Whole-genome single-cell copy number profiling from formalin-fixed paraffin-embedded samples
    Article Snippet: Paragraph title: Nuclear DNA extraction and Multiplex-PCR assay ... Fifty nanograms of DNA were subjected to template restoration using PreCR Repair Mix (NEB) in a reaction containing 1× ThermoPol Buffer, 100 μM dNTPs, 1× NAD+ , 1 μl of PreCR mix and H2 O to 50 μl, incubated 20 min at 37°C.

    In Vivo:

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: In brief, to identify each candidate TF binding maps in vivo , the DNA bound to each candidate TF from formaldehyde cross-linked E. coli cells were isolated by chromatin immunoprecipitation (ChIP) with the specific antibodies that specifically recognize myc tag (9E10, Santa Cruz Biotechnology), and Dynabeads Pan Mouse IgG magnetic beads (Invitrogen) followed by stringent washings as described previously ( ). .. Nick repair was performed by using PreCR Repair Mix (New England Biolabs).

    Magnetic Beads:

    Article Title: Rapid antibiotic-resistance predictions from genome sequence data for Staphylococcus aureus and Mycobacterium tuberculosis
    Article Snippet: Without undergoing any shearing process, 2 μg of DNA were treated with PreCR Repair Mix (New England BioLabs, NEB) , to repair possible damage to the DNA that could interfere with the sequencing process. .. The purified DNA from each of the two reactions was eluted from the magnetic beads in 40 μl EB buffer and pooled together.

    Article Title: Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers
    Article Snippet: The fragments were repaired with PreCR Repair Mix (New England BioLabs, Frankfurt, Germany) and stored in 10 mM PBS buffer (137 mM NaCl + 2.7 mM KCl (pH 7.4) at 25°C) at 4°C. .. As a handle, streptavidin-coated magnetic beads with a diameter of 1 μ m (Dynabeads MyOne Streptavidin C1, Life Technologies, Carlsbad, CA) were attached to the other DNA side.

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: In brief, to identify each candidate TF binding maps in vivo , the DNA bound to each candidate TF from formaldehyde cross-linked E. coli cells were isolated by chromatin immunoprecipitation (ChIP) with the specific antibodies that specifically recognize myc tag (9E10, Santa Cruz Biotechnology), and Dynabeads Pan Mouse IgG magnetic beads (Invitrogen) followed by stringent washings as described previously ( ). .. Nick repair was performed by using PreCR Repair Mix (New England Biolabs).

    Article Title: Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)
    Article Snippet: DNA was eluted from the beads by addition of 20 μl of TE buffer and incubation for 2 min in a ThermoMixer C at 1400 rpm 2 μl of PreCR Repair Mix (New England Biolabs) and 5 μl of NEBNext End Repair Reaction Buffer (New England Biolabs) in a 50 μl of total volume reaction was added to repair the damaged DNA template. .. The reaction was incubated at 37 °C for 20 min. DNA was end-repaired by addition of 2.5 μl of NEBNext End repair mix (New England Biolabs) (incubation at 25 °C for 5 min) and then purified with 0.45× volume of AMPure PB magnetic beads and eluted with 20 μl of TE.

    Isolation:

    Article Title: Genetic Variability of Microcystin Biosynthesis Genes in Planktothrix as Elucidated from Samples Preserved by Heat Desiccation during Three Decades
    Article Snippet: .. Aliquots of DNA isolated from four heat-desiccated samples from 1980, 1982, 1986, and 1988 were treated with the PreCR Repair Mix (37°C, 20 min). .. The efficiency of the PreCR Repair Mix was again tested by means of qPCR (see below).

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: In brief, to identify each candidate TF binding maps in vivo , the DNA bound to each candidate TF from formaldehyde cross-linked E. coli cells were isolated by chromatin immunoprecipitation (ChIP) with the specific antibodies that specifically recognize myc tag (9E10, Santa Cruz Biotechnology), and Dynabeads Pan Mouse IgG magnetic beads (Invitrogen) followed by stringent washings as described previously ( ). .. Nick repair was performed by using PreCR Repair Mix (New England Biolabs).

    Multiplex PCR:

    Article Title: Whole-genome single-cell copy number profiling from formalin-fixed paraffin-embedded samples
    Article Snippet: Paragraph title: Nuclear DNA extraction and Multiplex-PCR assay ... Fifty nanograms of DNA were subjected to template restoration using PreCR Repair Mix (NEB) in a reaction containing 1× ThermoPol Buffer, 100 μM dNTPs, 1× NAD+ , 1 μl of PreCR mix and H2 O to 50 μl, incubated 20 min at 37°C.

    Purification:

    Article Title: Robust long-read native DNA sequencing using the ONT CsgG Nanopore system
    Article Snippet: .. Damage to DNA was then repaired using the preCR repair mix (NEB)**, according to manufacturer recommendations and DNA subsequently purified using AMPureXP beads (Beckman Coulter). .. End-repair of DNA fragments was then performed using the Ultra II End Prep module (NEB).

    Article Title: Rapid antibiotic-resistance predictions from genome sequence data for Staphylococcus aureus and Mycobacterium tuberculosis
    Article Snippet: Without undergoing any shearing process, 2 μg of DNA were treated with PreCR Repair Mix (New England BioLabs, NEB) , to repair possible damage to the DNA that could interfere with the sequencing process. .. The repaired DNA was purified with 1 volume (100 μl) Agencourt AMPure XP beads (Beckman Coulter, UK) according to manufacturer's instructions.

    Article Title: CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells
    Article Snippet: IP-enriched chromatin was eluted with 100 mM NaHCO3 and 1% SDS at 65°C for 2 h. To reverse cross-linking, NaCl was added to the final 200 mM concentration and incubated at 67°C for additional 4 h followed by RNase A (150 μg/ml) treatment for 1 h and then proteins were digested with proteinase K (100 μg/ml) for 3 h. The DNA was purified by phenol extraction and ethanol precipitated. .. The DNA was repaired using PreCR Repair mix (New England Biolabs, Ipswich, MA, USA) following manufacturer’s instructions.

    Article Title: Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)
    Article Snippet: DNA was eluted from the beads by addition of 20 μl of TE buffer and incubation for 2 min in a ThermoMixer C at 1400 rpm 2 μl of PreCR Repair Mix (New England Biolabs) and 5 μl of NEBNext End Repair Reaction Buffer (New England Biolabs) in a 50 μl of total volume reaction was added to repair the damaged DNA template. .. The reaction was incubated at 37 °C for 20 min. DNA was end-repaired by addition of 2.5 μl of NEBNext End repair mix (New England Biolabs) (incubation at 25 °C for 5 min) and then purified with 0.45× volume of AMPure PB magnetic beads and eluted with 20 μl of TE.

    Sequencing:

    Article Title: Robust long-read native DNA sequencing using the ONT CsgG Nanopore system
    Article Snippet: Paragraph title: Library preparation and sequencing ... Damage to DNA was then repaired using the preCR repair mix (NEB)**, according to manufacturer recommendations and DNA subsequently purified using AMPureXP beads (Beckman Coulter).

    Article Title: PacBio-LITS: a large-insert targeted sequencing method for characterization of human disease-associated chromosomal structural variations
    Article Snippet: With continuous advances in sequencing technology (e.g. improvement of read length and error rate), it is possible in the foreseeable future that libraries with even larger inserts will be much desired for SV studies. .. PreCR Repair Mix by New England Biolabs) could be incorporated into the protocol.

    Article Title: Rapid antibiotic-resistance predictions from genome sequence data for Staphylococcus aureus and Mycobacterium tuberculosis
    Article Snippet: .. Without undergoing any shearing process, 2 μg of DNA were treated with PreCR Repair Mix (New England BioLabs, NEB) , to repair possible damage to the DNA that could interfere with the sequencing process. .. Following Oxford Nanopore recommendation for the optional PreCR treatment, each μg of DNA was first diluted in nuclease-free water to a volume of 85 μl to which 10 μl ThermoPol Reaction Buffer, 1 μl NAD+ , 1 μl 10 μM dNTPs and 2 μl PreCR Repair Mix were added, and the two reactions were incubated at 37 °C for 30 min.

    Article Title: Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)
    Article Snippet: Paragraph title: Pacific Biosciences sequencing of fosmid clones ... DNA was eluted from the beads by addition of 20 μl of TE buffer and incubation for 2 min in a ThermoMixer C at 1400 rpm 2 μl of PreCR Repair Mix (New England Biolabs) and 5 μl of NEBNext End Repair Reaction Buffer (New England Biolabs) in a 50 μl of total volume reaction was added to repair the damaged DNA template.

    Chromatin Immunoprecipitation:

    Article Title: Systematic discovery of uncharacterized transcription factors in Escherichia coli K-12 MG1655
    Article Snippet: Paragraph title: ChIP-exo experiment ... Nick repair was performed by using PreCR Repair Mix (New England Biolabs).

    Article Title: CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells
    Article Snippet: Paragraph title: Antibodies used for chromatin immunoprecipitation, western blot, and immunofluorescence ... The DNA was repaired using PreCR Repair mix (New England Biolabs, Ipswich, MA, USA) following manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus
    Article Snippet: Linear DNase and Restriction Endonuclease Treatment of exDNA Samples and End-Point PCR of DNA Targets Prior to linear DNase and/or treatment with restriction endonuclease, exDNA samples were treated with PreCR Repair Mix (NEB) to repair nicked DNA. .. PreCR-treated samples were then either (1) treated with the ϕSa4ms-specific restriction endonucleases Psh AI and Psp XI (NEB) following manufacturer’s protocol, (2) treated with Plasmid-Safe-ATP-Dependent DNase (Epicentre), (3) treated with Psh AI and Psp XI, then the Plasmid-Safe-ATP-Dependent DNase, or (4) left solely PreCR-treated.

    Sample Prep:

    Article Title: Nanomechanics of Fluorescent DNA Dyes on DNA Investigated by Magnetic Tweezers
    Article Snippet: Paragraph title: DNA sample preparation ... The fragments were repaired with PreCR Repair Mix (New England BioLabs, Frankfurt, Germany) and stored in 10 mM PBS buffer (137 mM NaCl + 2.7 mM KCl (pH 7.4) at 25°C) at 4°C.

    In Situ:

    Article Title: CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells
    Article Snippet: Chromatin IP for CHIP-Seq was performed similarly as above, except for the following modifications: MNase concentration was 0.6U/ml, digestion time was 10 min for cancer cells and 8 min for normal HcoEpiC cells, and nuclei were treated with 0.05 to 0.1% formaldehyde for gentle in situ crosslinking within intact nuclei for 30 min at RT, as indicated in ENCODE protocols, before extraction of chromatin in low salt buffer. .. The DNA was repaired using PreCR Repair mix (New England Biolabs, Ipswich, MA, USA) following manufacturer’s instructions.

    Concentration Assay:

    Article Title: Genetic Variability of Microcystin Biosynthesis Genes in Planktothrix as Elucidated from Samples Preserved by Heat Desiccation during Three Decades
    Article Snippet: .. Evaluation of DNA treatment to reverse DNA modifications Four randomly selected DNA samples were treated with the PreCR Repair Mix and the DNA template concentration as estimated by qPCR was compared quantitatively to untreated aliquots for four different loci (16Sr DNA, Dhb, Mdha, and Hty). ..

    Article Title: CENP-A nucleosomes localize to transcription factor hotspots and subtelomeric sites in human cancer cells
    Article Snippet: IP-enriched chromatin was eluted with 100 mM NaHCO3 and 1% SDS at 65°C for 2 h. To reverse cross-linking, NaCl was added to the final 200 mM concentration and incubated at 67°C for additional 4 h followed by RNase A (150 μg/ml) treatment for 1 h and then proteins were digested with proteinase K (100 μg/ml) for 3 h. The DNA was purified by phenol extraction and ethanol precipitated. .. The DNA was repaired using PreCR Repair mix (New England Biolabs, Ipswich, MA, USA) following manufacturer’s instructions.

    Nanopore Sequencing:

    Article Title: Rapid antibiotic-resistance predictions from genome sequence data for Staphylococcus aureus and Mycobacterium tuberculosis
    Article Snippet: Paragraph title: Nanopore sequencing ... Without undergoing any shearing process, 2 μg of DNA were treated with PreCR Repair Mix (New England BioLabs, NEB) , to repair possible damage to the DNA that could interfere with the sequencing process.

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  • 95
    New England Biolabs precr repair mix
    Number of substitutions found in gene loci used for qPCR and detected by sequencing from heat-desiccated <t>DNA,</t> an aliquot treated with the <t>PreCR</t> Repair Mix, and DNA from a frozen sample. (A) Number of substitutions; (B) number of substitutions in the primer sites (per 100 bp). *, no substitutions detected; nd, no data.
    Precr Repair Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/precr repair mix/product/New England Biolabs
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    precr repair mix - by Bioz Stars, 2020-02
    95/100 stars
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    Number of substitutions found in gene loci used for qPCR and detected by sequencing from heat-desiccated DNA, an aliquot treated with the PreCR Repair Mix, and DNA from a frozen sample. (A) Number of substitutions; (B) number of substitutions in the primer sites (per 100 bp). *, no substitutions detected; nd, no data.

    Journal: PLoS ONE

    Article Title: Genetic Variability of Microcystin Biosynthesis Genes in Planktothrix as Elucidated from Samples Preserved by Heat Desiccation during Three Decades

    doi: 10.1371/journal.pone.0080177

    Figure Lengend Snippet: Number of substitutions found in gene loci used for qPCR and detected by sequencing from heat-desiccated DNA, an aliquot treated with the PreCR Repair Mix, and DNA from a frozen sample. (A) Number of substitutions; (B) number of substitutions in the primer sites (per 100 bp). *, no substitutions detected; nd, no data.

    Article Snippet: Evaluation of DNA treatment to reverse DNA modifications Four randomly selected DNA samples were treated with the PreCR Repair Mix and the DNA template concentration as estimated by qPCR was compared quantitatively to untreated aliquots for four different loci (16Sr DNA, Dhb, Mdha, and Hty).

    Techniques: Real-time Polymerase Chain Reaction, Sequencing