rnase inhibitor human placenta  (New England Biolabs)


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    Name:
    RNase Inhibitor Human Placenta
    Description:
    RNase Inhibitor Human Placenta 10 000 units
    Catalog Number:
    M0307L
    Price:
    323
    Category:
    Enzyme Inhibitors
    Size:
    10 000 units
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    Structured Review

    New England Biolabs rnase inhibitor human placenta
    RNase Inhibitor Human Placenta
    RNase Inhibitor Human Placenta 10 000 units
    https://www.bioz.com/result/rnase inhibitor human placenta/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase inhibitor human placenta - by Bioz Stars, 2021-05
    97/100 stars

    Images

    1) Product Images from "Comparison of SARS-CoV-2 Indirect and Direct Detection Methods"

    Article Title: Comparison of SARS-CoV-2 Indirect and Direct Detection Methods

    Journal: bioRxiv

    doi: 10.1101/2020.05.12.092387

    One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.
    Figure Legend Snippet: One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.

    Techniques Used: RNA Extraction, Lysis, Quantitative RT-PCR, SYBR Green Assay

    Related Articles

    Synthesized:

    Article Title: The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties
    Article Snippet: Total RNA was extracted using the total RNA isolation mini kit (Agilent Technologies Ltd.). .. All samples were treated with DNAse on the columns using RNase-free DNase I provided in the kit. cDNA was synthesized from 0.2-1 μg of total RNA using 200 U MuLV reverse transcriptase (New England Biolabs), 40 U RNase inhibitor (human placenta) (New England Biolabs), 0.5 mM dNTP, 25 μM oligo-dT primers, and 10× reverse transcriptase buffer (500 mM Tris–HCl pH 8.3, 750 mM KCl, 30 mM MgCl2 , 100 mM DTT) (New England Biolabs) in a final reaction volume 20 μl with added nuclease-free water as required (Qiagen). .. Hot Start Taq 2× master mix (New England Biolabs) was used for standard PCR.

    other:

    Article Title: Helicase-dependent RNA decay illuminated by a cryo-EM structure of a human nuclear RNA exosome-MTR4 complex
    Article Snippet: Assays in main were carried out at 22°C in a buffer containing 20 mM Tris-HCl pH 7.0, 50 mM NaCl, 0.5 mM MgCl2 , 5 mM BME, and 1 U/μl RNAse inhibitor, human placenta (New England Biolabs).

    Incubation:

    Article Title: Differential regulation of LncRNA-SARCC suppresses VHL-mutant RCC cell proliferation yet promotes VHL-normal RCC cell proliferation via modulating androgen receptor/HIF-2α/C-MYC axis under hypoxia.
    Article Snippet: .. It is well established that hypoxia contributes to tumor progression in a hypoxia inducible factor-2α (HIF-2α)-dependent manner in renal cell carcinoma (RCC), yet the role of long noncoding RNAs (LncRNAs) involved in hypoxia-mediated RCC progression remains unclear. .. It is well established that hypoxia contributes to tumor progression in a hypoxia inducible factor-2α (HIF-2α)-dependent manner in renal cell carcinoma (RCC), yet the role of long noncoding RNAs (LncRNAs) involved in hypoxia-mediated RCC progression remains unclear.

    Protease Inhibitor:

    Article Title: Differential regulation of LncRNA-SARCC suppresses VHL-mutant RCC cell proliferation yet promotes VHL-normal RCC cell proliferation via modulating androgen receptor/HIF-2α/C-MYC axis under hypoxia.
    Article Snippet: .. It is well established that hypoxia contributes to tumor progression in a hypoxia inducible factor-2α (HIF-2α)-dependent manner in renal cell carcinoma (RCC), yet the role of long noncoding RNAs (LncRNAs) involved in hypoxia-mediated RCC progression remains unclear. .. It is well established that hypoxia contributes to tumor progression in a hypoxia inducible factor-2α (HIF-2α)-dependent manner in renal cell carcinoma (RCC), yet the role of long noncoding RNAs (LncRNAs) involved in hypoxia-mediated RCC progression remains unclear.

    In Vitro:

    Article Title: Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour
    Article Snippet: PCR products were purified with Wizard Plus SV Gel and PCR Clean-Up Systems (Promega). .. Transcription–translation reactions used the PURExpress In Vitro Protein Synthesis Kit (New England Biolabs) supplemented with 20 units of Human Placenta RNase Inhibitor (New England Biolabs). .. Reactions were monitored by fluorescence with a CFX96 Touch real-time PCR (Bio-Rad) using the SYBR green filter set.

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    New England Biolabs rnase inhibitor human placenta
    One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the <t>RNase</t> inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) <t>RT-qPCR</t> detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.
    Rnase Inhibitor Human Placenta, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase inhibitor human placenta/product/New England Biolabs
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rnase inhibitor human placenta - by Bioz Stars, 2021-05
    97/100 stars
      Buy from Supplier

    Image Search Results


    One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.

    Journal: bioRxiv

    Article Title: Comparison of SARS-CoV-2 Indirect and Direct Detection Methods

    doi: 10.1101/2020.05.12.092387

    Figure Lengend Snippet: One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.

    Article Snippet: With direct RT-qPCR we found that simply adding RNase inhibitor greatly improved sensitivity, without need for any other treatments (e.g. lysis buffers or boiling).

    Techniques: RNA Extraction, Lysis, Quantitative RT-PCR, SYBR Green Assay