rnase inhibitor human placenta  (New England Biolabs)


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    Name:
    RNase Inhibitor Human Placenta
    Description:
    RNase Inhibitor Human Placenta 10 000 units
    Catalog Number:
    m0307l
    Price:
    323
    Size:
    10 000 units
    Category:
    Enzyme Inhibitors
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    Structured Review

    New England Biolabs rnase inhibitor human placenta
    RNase Inhibitor Human Placenta
    RNase Inhibitor Human Placenta 10 000 units
    https://www.bioz.com/result/rnase inhibitor human placenta/product/New England Biolabs
    Average 98 stars, based on 208 article reviews
    Price from $9.99 to $1999.99
    rnase inhibitor human placenta - by Bioz Stars, 2020-10
    98/100 stars

    Images

    1) Product Images from "Comparison of SARS-CoV-2 Indirect and Direct Detection Methods"

    Article Title: Comparison of SARS-CoV-2 Indirect and Direct Detection Methods

    Journal: bioRxiv

    doi: 10.1101/2020.05.12.092387

    One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.
    Figure Legend Snippet: One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.

    Techniques Used: RNA Extraction, Lysis, Quantitative RT-PCR, SYBR Green Assay

    Related Articles

    Labeling:

    Article Title: Structural basis for MTR4–ZCCHC8 interactions that stimulate the MTR4 helicase in the nuclear exosome-targeting complex
    Article Snippet: .. Proteins (200 nM) were incubated with 100 nM RNA (A20 ) labeled with internal 4-thiouridine (4SU) in the indicated position in a buffer containing 20 mM Tris, pH 7.0, 50 mM NaCl, 5 mM BME, 1 U/µL RNase inhibitor, human placenta (New England Biolabs), 1.5 mM MgCl2 , and 1 mM AMPPNP (pH 7.0) at 22 °C for 30 min. Cross-linking was induced by exposure of the protein–RNA mixture to 365 nm UV light using a 4-W handheld UV lamp (UVP) for 20 min at 4 °C. .. Samples were quenched with NuPAGE lithium dodecyl sulfate sample buffer (Thermo Fisher Scientific) and subjected to SDS/PAGE analysis using NuPAGE 4 to 12% Bis-Tris protein gels (Thermo Fisher Scientific).

    Purification:

    Article Title: Data on the inhibition of RNase inhibitor activity by a monoclonal antibody as assessed by microfluidics-based RNA electrophoresis
    Article Snippet: .. 2.2 Inhibition of RI activity by a monoclonal Ab specific to RI (3F11) Purified total RNA from Vero cells (5 µg in 200 µL of CL Buffer) was mixed with 1 µg (1 µL) of monoclonal Ab specific to either RI (Origene; TA501875; clone 3F11) or GAPDH (Origene; TA802519; clone 2D9), 1 ng (1 µL) of RNase A (Qiagen; 19101; diluted in CL Buffer), 40 units (1 µL) of human placental RI (hpRI; New England Biolabs; M0307), or a combination of the above; in reactions containing hpRI, its addition preceded the addition of other components. .. Following the incubation, RNA was purified and subjected to Experion analysis.

    Incubation:

    Article Title: Structural basis for MTR4–ZCCHC8 interactions that stimulate the MTR4 helicase in the nuclear exosome-targeting complex
    Article Snippet: .. Proteins (200 nM) were incubated with 100 nM RNA (A20 ) labeled with internal 4-thiouridine (4SU) in the indicated position in a buffer containing 20 mM Tris, pH 7.0, 50 mM NaCl, 5 mM BME, 1 U/µL RNase inhibitor, human placenta (New England Biolabs), 1.5 mM MgCl2 , and 1 mM AMPPNP (pH 7.0) at 22 °C for 30 min. Cross-linking was induced by exposure of the protein–RNA mixture to 365 nm UV light using a 4-W handheld UV lamp (UVP) for 20 min at 4 °C. .. Samples were quenched with NuPAGE lithium dodecyl sulfate sample buffer (Thermo Fisher Scientific) and subjected to SDS/PAGE analysis using NuPAGE 4 to 12% Bis-Tris protein gels (Thermo Fisher Scientific).

    Article Title: RNA Sequences Required for the Noncoding Function of oskar RNA also Mediate Regulation of Oskar Protein Expression by Bicoid Stability Factor
    Article Snippet: .. The samples were incubated for 1 hr at 37 °C with 2.5 U of RNase H (NEB) and 20 U of RNase Inhibitor, Human Placenta (HPRI, NEB) in 50 μl of RNase H buffer (NEB). .. Decapped RNAs were purified with RNeasy MiniElute Cleanup Kit (Qiagen).

    Inhibition:

    Article Title: Data on the inhibition of RNase inhibitor activity by a monoclonal antibody as assessed by microfluidics-based RNA electrophoresis
    Article Snippet: .. 2.2 Inhibition of RI activity by a monoclonal Ab specific to RI (3F11) Purified total RNA from Vero cells (5 µg in 200 µL of CL Buffer) was mixed with 1 µg (1 µL) of monoclonal Ab specific to either RI (Origene; TA501875; clone 3F11) or GAPDH (Origene; TA802519; clone 2D9), 1 ng (1 µL) of RNase A (Qiagen; 19101; diluted in CL Buffer), 40 units (1 µL) of human placental RI (hpRI; New England Biolabs; M0307), or a combination of the above; in reactions containing hpRI, its addition preceded the addition of other components. .. Following the incubation, RNA was purified and subjected to Experion analysis.

    Activity Assay:

    Article Title: Data on the inhibition of RNase inhibitor activity by a monoclonal antibody as assessed by microfluidics-based RNA electrophoresis
    Article Snippet: .. 2.2 Inhibition of RI activity by a monoclonal Ab specific to RI (3F11) Purified total RNA from Vero cells (5 µg in 200 µL of CL Buffer) was mixed with 1 µg (1 µL) of monoclonal Ab specific to either RI (Origene; TA501875; clone 3F11) or GAPDH (Origene; TA802519; clone 2D9), 1 ng (1 µL) of RNase A (Qiagen; 19101; diluted in CL Buffer), 40 units (1 µL) of human placental RI (hpRI; New England Biolabs; M0307), or a combination of the above; in reactions containing hpRI, its addition preceded the addition of other components. .. Following the incubation, RNA was purified and subjected to Experion analysis.

    Quantitative RT-PCR:

    Article Title: Comparison of SARS-CoV-2 Indirect and Direct Detection Methods
    Article Snippet: .. With direct RT-qPCR we found that simply adding RNase inhibitor greatly improved sensitivity, without need for any other treatments (e.g. lysis buffers or boiling). ..

    Lysis:

    Article Title: Comparison of SARS-CoV-2 Indirect and Direct Detection Methods
    Article Snippet: .. With direct RT-qPCR we found that simply adding RNase inhibitor greatly improved sensitivity, without need for any other treatments (e.g. lysis buffers or boiling). ..

    other:

    Article Title: HNRNPM controls circRNA biogenesis and splicing fidelity to sustain prostate cancer cell fitness
    Article Snippet: Reaction was started by addition of 4 μl 5X RT Buffer [250 mM Tris-HCl, pH 8.3; 375 mM KCl; 15 mM MgCl2 ], 1 μl 0.1 M DTT, 20 U SUPERase• In™ RNase Inhibitor, and 200 U TGIRT™ -III Enzyme.

    Article Title: Genome-wide mapping of therapeutically-relevant SARS-CoV-2 RNA structures
    Article Snippet: Reactions were supplemented with 1 μl 5X RT Buffer [250 mM Tris-HCl pH 8.3; 375 mM KCl], 0.5 μl DTT 0.1 M, 0.25 μl MnCl2 120 mM, 5 U SUPERase•In™ RNase Inhibitor and 50 U SuperScript II RT.

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  • 98
    New England Biolabs rnase inhibitor human placenta
    One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the <t>RNase</t> inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) <t>RT-qPCR</t> detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.
    Rnase Inhibitor Human Placenta, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnase inhibitor human placenta/product/New England Biolabs
    Average 98 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    rnase inhibitor human placenta - by Bioz Stars, 2020-10
    98/100 stars
      Buy from Supplier

    Image Search Results


    One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.

    Journal: bioRxiv

    Article Title: Comparison of SARS-CoV-2 Indirect and Direct Detection Methods

    doi: 10.1101/2020.05.12.092387

    Figure Lengend Snippet: One-step direct detection without RNA extraction. (A) Analysis of extracted RNA or direct UTM from a panel of patients using the BGI or Norgen (N1 and N2 primers) detection systems. (B) Comparison of Ct values from clinical lab analysis on extracted RNA (E, RdRp and N genes) to data obtained for direct analysis with the BGI detection system. (C) Patient samples in UTM were left untreated, or treated with the RNase inhibitor RNaseOUT with or without heating at 95°C for 15 min, or treated with the indicated lysis buffers/detergents and then directly analyzed using the BGI or Norgen (N1/N2 primers) RT-qPCR detection systems. Note sample L020 (clinical negative) was also tested under these conditions and was confirmed as SARS-CoV-2 negative. (D) Cost analysis comparing Norgen, BGI, and SYBR green systems. Price is in CAD at the time these studies were initiated (late March/early April 2020) for 10µl RT-qPCR reactions and include relevant processing and shipping fees. * BGI RNA extraction module is based on the 96-sample format, price can be reduced ∼15% by purchasing the 1728-sample format, and bulk pricing with a ∼25% discount of the detection module is available for > 10,000 samples. ** Pricing for the Norgen detection module is based on the 50-sample format running three separate wells (N1, N2 and RNaseP) per sample, pricing can be reduced if purchasing the larger 500-sample format. *** Pricing for SYBR green detection is based on the 200 reaction size LUNA Universal One-Step RT-qPCR Kit (NEB) running three separate wells/sample (two viral genes and one human control gene). Pricing can be reduced up to 30% with larger kit sizes. N/A: not applicable.

    Article Snippet: With direct RT-qPCR we found that simply adding RNase inhibitor greatly improved sensitivity, without need for any other treatments (e.g. lysis buffers or boiling).

    Techniques: RNA Extraction, Lysis, Quantitative RT-PCR, SYBR Green Assay