endonuclease  (New England Biolabs)


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    Name:
    Endonuclease V
    Description:
    Endonuclease V 250 units
    Catalog Number:
    m0305s
    Price:
    75
    Size:
    250 units
    Category:
    Other Endonucleases
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    Structured Review

    New England Biolabs endonuclease
    Endonuclease V
    Endonuclease V 250 units
    https://www.bioz.com/result/endonuclease/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    endonuclease - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons
    Article Snippet: .. Using standard cloning techniques and endonuclease (5′, EcoRI; 3′, Hin dIII) sequence-tagged primers, Sync1-Sync3, Per45 and Per58 were subcloned from pcDNA3 into pMAL (New England Biolabs) using standard methodologies. .. Syncoilin N-term (residues 1-157) and rod domains (residues 158-452) were subcloned into pMAL using the following primers: N-termF, TTT GAA TTC ATG GCC AGC CCG GAA CCC CT; N-termR, TTT AAG CTT AGA GGG GAT CCT CCT CGG TGT; RodF, TTT GAA TTC AGC GTG GAG GAC CTG GAG CG; RodR, TTT AAG CTT ATG CAT CAG CCT GTT CCA GAC.

    Amplification:

    Article Title: Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)
    Article Snippet: 16S-23S rDNA spacer amplicons were digested with 4 U of each endonuclease (New England BioLabs, Saint-Quentin-en-Yvelines, France) and fractionated by electrophoresis in 2.5% high-resolution agarose gels (MetaPhor; TEBU, Le Perray en Yvelines, France) at 100 V for 3 h. With PCR-ribotyping, all of the strains of R. paucula exhibited a single fragment of between 831 and 947 bp in size, based on the use of DNA Molecular Weight Marker III. .. 16S-23S rDNA spacer amplicons were digested with 4 U of each endonuclease (New England BioLabs, Saint-Quentin-en-Yvelines, France) and fractionated by electrophoresis in 2.5% high-resolution agarose gels (MetaPhor; TEBU, Le Perray en Yvelines, France) at 100 V for 3 h. With PCR-ribotyping, all of the strains of R. paucula exhibited a single fragment of between 831 and 947 bp in size, based on the use of DNA Molecular Weight Marker III.

    Article Title: Genetic Variation in Sialidase and Linkage to N-acetylneuraminate Catabolism in Mycoplasma synoviae
    Article Snippet: Amplification conditions, using GoTaq reagents (Promega, Madison, Wisconsin), were as described in section 4.2, with the exception of extension steps of 3 min at 72°C. .. Genomic DNA from the strains with the highest (WVU1853T ) and lowest (K4907A and K5395B) levels of sialidase activity was digested with endonuclease Vsp I (New England Biolabs, Ipswich, Massachusetts), then separated on a 0.6% agarose gel.

    Article Title: Antibiotic resistance, virulence determinants and production of biogenic amines among enterococci from ovine, feline, canine, porcine and human milk
    Article Snippet: Genetic profiling of the enterococcal isolates Initially, the enterococcal isolates were typed by Random Amplification of Polymorphic DNA (RAPD) in order to avoid duplication of isolates from a same host. .. Later, a representative of each RAPD profile found in each host was submitted to PFGE genotyping [ ]; for this purpose, chromosomal DNA was digested with the endonuclease Sma I (New England Biolabs, Ipswich, MA) at 37°C for 16 h. Then, electrophoresis was carried out in a CHEF DR-III apparatus (Bio-Rad) for 23 h at 14°C at 6 V/cm with pulses from 5 to 50 s. A standard pattern (Lamda Ladder PFG Marker, New England Biolabs) was included in the gels to compare the digitally normalized PFGE profiles.

    Article Title: Hydroxylation index of omeprazole in relation to CYP2C19 polymorphism and sex in a healthy Iranian population
    Article Snippet: Amplification of CYP2C19*2 and *3 allele was implemented using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as described by De Morias [ ]. .. The PCR product of each reaction was digested by specific endonuclease (all from New England Biolabs GmbH, Frankfurt, Germany); the 169 bp CYP2C19*2 product was digested by SmaI to 40 and 129 bp fragments.

    Article Title: Digital MDA for enumeration of total nucleic acid contamination
    Article Snippet: The crude products were also analyzed by agarose gel electrophoresis to assess the presence of high molecular weight amplified DNA. .. Endonuclease activity was tested by incubation of enzyme samples with 1 μg ϕ × 174 virion (ss, closed circular) DNA (New England Biolabs) at 37°C for 4 h in DNAse I reaction buffer (New England Biolabs).

    Article Title: Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
    Article Snippet: To study the epigenetic changes in specific DNA sequences it was used MS-RAPD, a modification of the original RAPD technique which is closer to MSAP (methylation sensitive amplified polymorphisms) analysis. .. In the first reaction, 250 ηg of each extracted DNA was added to a restriction mixture containing: 1× restriction buffer and 10/20 U restriction endonuclease to a final volume of 30 µL, one mixture per endonuclease (Hpa II/Msp I; all endonucleases and buffers were supplied by New England Biolabs, USA).

    Mass Spectrometry:

    Article Title: Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
    Article Snippet: Paragraph title: Methylation-sensitive random-amplified polymorphic DNA (MS-RAPD) ... In the first reaction, 250 ηg of each extracted DNA was added to a restriction mixture containing: 1× restriction buffer and 10/20 U restriction endonuclease to a final volume of 30 µL, one mixture per endonuclease (Hpa II/Msp I; all endonucleases and buffers were supplied by New England Biolabs, USA).

    Nucleic Acid Electrophoresis:

    Article Title: Uptake of Foreign Nucleic Acids in Kidney Tubular Epithelial Cells Deficient in Proapoptotic Endonucleases
    Article Snippet: Endonuclease activity was measured the same way as above in samples containing serially diluted protein (1:5), 1 μg plasmid pBR322 DNA (New England Biolabs), 2 mM CaCl2 , 5 mM MgCl2 , 10 mM Tris-HCl, pH 7.4, and 0.5 mM dithiothreitol to determine the Ca/Mg-dependent (primarily DNase I) endonuclease activity or 5 mM MnCl2 , 10 mM Tris-HCl (pH 7.4), and 0.5 mM dithiothreitol for the Mn-dependent (mainly EndoG) activity. .. As opposed to PIA, zymogram gel electrophoresis, previously used by us to assess DNase I activity (Basnakian et al ., ), was not applicable for EndoG due to low specific activity of the enzyme in the used tubular epithelial cells.

    Article Title: Small-Molecule Antioxidant Proteome-Shields in Deinococcus radiodurans
    Article Snippet: Following irradiation, 20 µl of each ionizing radiation-treated Bam HI sample were assayed for residual endonuclease activity in separate reaction mixtures (final volume, 30 µl) containing 125 ng μ-phage DNA, 50 mM NaCl, 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2 , and 1 mM dithiothreitol (New England Biolabs). .. Bam HI/μ DNA mixtures were incubated for 1.25 h at 37°C, followed by agarose (0.8%) gel electrophoresis (AGE).

    Article Title: Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
    Article Snippet: DNA yield and purity were assessed by spectrophotometry (as described in Valledor et al. ) and gel electrophoresis on agarose gel by direct comparison with phage λ DNA. .. In the first reaction, 250 ηg of each extracted DNA was added to a restriction mixture containing: 1× restriction buffer and 10/20 U restriction endonuclease to a final volume of 30 µL, one mixture per endonuclease (Hpa II/Msp I; all endonucleases and buffers were supplied by New England Biolabs, USA).

    Lambda DNA Preparation:

    Article Title: A Sequence-Specific Nicking Endonuclease from Streptomyces: Purification, Physical and Catalytic Properties
    Article Snippet: DNA Cleavage Assay Enzyme activity was measured by incubating the endonuclease with supercoiled plasmid or lambda DNA in buffer R (10 mM Tris-HCl (pH 8.0), 10 mM MgCl2 , 50 mM NaCl, and 5 mM β -mercaptoethanol). .. The unit of endonuclease activity was quantified by titrating against known units of commercial Eco RI (New England Biolabs).

    Electrophoresis:

    Article Title: Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)
    Article Snippet: .. 16S-23S rDNA spacer amplicons were digested with 4 U of each endonuclease (New England BioLabs, Saint-Quentin-en-Yvelines, France) and fractionated by electrophoresis in 2.5% high-resolution agarose gels (MetaPhor; TEBU, Le Perray en Yvelines, France) at 100 V for 3 h. With PCR-ribotyping, all of the strains of R. paucula exhibited a single fragment of between 831 and 947 bp in size, based on the use of DNA Molecular Weight Marker III. ..

    Article Title: Antibiotic resistance, virulence determinants and production of biogenic amines among enterococci from ovine, feline, canine, porcine and human milk
    Article Snippet: .. Later, a representative of each RAPD profile found in each host was submitted to PFGE genotyping [ ]; for this purpose, chromosomal DNA was digested with the endonuclease Sma I (New England Biolabs, Ipswich, MA) at 37°C for 16 h. Then, electrophoresis was carried out in a CHEF DR-III apparatus (Bio-Rad) for 23 h at 14°C at 6 V/cm with pulses from 5 to 50 s. A standard pattern (Lamda Ladder PFG Marker, New England Biolabs) was included in the gels to compare the digitally normalized PFGE profiles. .. Computer-assisted analysis was performed with the Phoretix 1D Pro software (Nonlinear USA, Inc., Durham, NC).

    Article Title: Staphylococcus epidermidis strains isolated from breast milk of women suffering infectious mastitis: potential virulence traits and resistance to antibiotics
    Article Snippet: .. Chromosomal DNA was digested with the endonuclease Sma I (New England Biolabs, Ipswich, MA) at 37°C for 16 h. Electrophoresis was carried out in a CHEF DR-III apparatus (Bio-Rad Laboratories, Hercules, CA) for 23 h at 14°C at 6 V cm-1 with pulses from 5 to 50 s. A standard pattern (Lamda Ladder PFG Marker, New England Biolabs) was included in the gels to allow comparison of the digitally normalized PFGE profiles. .. Computer-assisted analysis of the gels was performed with the Phoretix 1D Pro software (Nonlinear USA, Inc., Durham, NC).

    Article Title: Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
    Article Snippet: In the first reaction, 250 ηg of each extracted DNA was added to a restriction mixture containing: 1× restriction buffer and 10/20 U restriction endonuclease to a final volume of 30 µL, one mixture per endonuclease (Hpa II/Msp I; all endonucleases and buffers were supplied by New England Biolabs, USA). .. The reaction was stopped by placing the tubes on ice and restriction was checked by agarose electrophoresis.

    Incubation:

    Article Title: A Sequence-Specific Nicking Endonuclease from Streptomyces: Purification, Physical and Catalytic Properties
    Article Snippet: After 30 min of incubation, the reactions were quenched by addition of 1/3 volume of loading dye solution containing 0.5% SDS and heating at 80°C for 5 min. Cleavage products were analyzed on 1 or 1.5% agarose gels. .. The unit of endonuclease activity was quantified by titrating against known units of commercial Eco RI (New England Biolabs).

    Article Title: Uptake of Foreign Nucleic Acids in Kidney Tubular Epithelial Cells Deficient in Proapoptotic Endonucleases
    Article Snippet: After adding serially diluted samples (1:5) to the reaction mixture (1 μg pBR322 plasmid DNA, 2 mM CaCl2 , 5 mM MgCl2 , 10 mM Tris-HCl, pH 7.4, and 0.5 mM dithiothreitol) the reaction was incubated for 1 h at 37°C, after which the reaction was terminated by adding Stop-solution (10 mM Tris-HCl, pH 7.4, 1% SDS, 25 mM Na2 EDTA, 7.5 mM bromophenol blue). .. Endonuclease activity was measured the same way as above in samples containing serially diluted protein (1:5), 1 μg plasmid pBR322 DNA (New England Biolabs), 2 mM CaCl2 , 5 mM MgCl2 , 10 mM Tris-HCl, pH 7.4, and 0.5 mM dithiothreitol to determine the Ca/Mg-dependent (primarily DNase I) endonuclease activity or 5 mM MnCl2 , 10 mM Tris-HCl (pH 7.4), and 0.5 mM dithiothreitol for the Mn-dependent (mainly EndoG) activity.

    Article Title: Inosine modifications in human tRNAs are incorporated at the precursor tRNA level
    Article Snippet: .. The Endonuclease V (EndoV) assay was performed by incubating 1 μg in vitro transcribed pre-tRNAVal (AAC) (incubated or not with hetADAT) with 1 μl EndoV (New England Biolabs) in a 50-μl reaction containing buffer 4 (New England Biolabs) and DEPC-treated water, overnight at 37°C. .. RNA was then dephosphorylated by addition of 1 μl Calf Intestinal Phosphatase (New England Biolabs) to each EndoV reaction and incubation for 1 h at 37°C.

    Article Title: Digital MDA for enumeration of total nucleic acid contamination
    Article Snippet: .. Endonuclease activity was tested by incubation of enzyme samples with 1 μg ϕ × 174 virion (ss, closed circular) DNA (New England Biolabs) at 37°C for 4 h in DNAse I reaction buffer (New England Biolabs). .. DNA shearing Twenty nanograms E. coli K12 genomic DNA [again prepared by the DNeasy method (Qiagen) for Gram-negative cells] was diluted in 200 μl 20 mM Tris, pH 7.5, containing 0.05% Tween-20.

    Article Title: Uptake of Foreign Nucleic Acids in Kidney Tubular Epithelial Cells Deficient in Proapoptotic Endonucleases
    Article Snippet: Endonuclease activity in total protein extracts from kidney cells and in culture medium was determined using the plasmid incision assay (PIA) with pBR322 plasmid (New England Biolabs, Beverly, MA) as the substrate as described previously (Basnakian et al ., ). .. Plasmid and Lipofectamine were diluted separately in serum-free DMEM/Ham's F-12 medium (Sigma-Aldrich), mixed together, and incubated for 20 min at room temperature.

    Article Title: Small-Molecule Antioxidant Proteome-Shields in Deinococcus radiodurans
    Article Snippet: Following irradiation, 20 µl of each ionizing radiation-treated Bam HI sample were assayed for residual endonuclease activity in separate reaction mixtures (final volume, 30 µl) containing 125 ng μ-phage DNA, 50 mM NaCl, 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2 , and 1 mM dithiothreitol (New England Biolabs). .. Bam HI/μ DNA mixtures were incubated for 1.25 h at 37°C, followed by agarose (0.8%) gel electrophoresis (AGE).

    Activity Assay:

    Article Title: A Sequence-Specific Nicking Endonuclease from Streptomyces: Purification, Physical and Catalytic Properties
    Article Snippet: .. The unit of endonuclease activity was quantified by titrating against known units of commercial Eco RI (New England Biolabs). .. Protein concentration was determined by the Bradford method using BioRad protein assay reagent (BioRad, USA) and BSA as the standard.

    Article Title: Uptake of Foreign Nucleic Acids in Kidney Tubular Epithelial Cells Deficient in Proapoptotic Endonucleases
    Article Snippet: .. Endonuclease activity was measured the same way as above in samples containing serially diluted protein (1:5), 1 μg plasmid pBR322 DNA (New England Biolabs), 2 mM CaCl2 , 5 mM MgCl2 , 10 mM Tris-HCl, pH 7.4, and 0.5 mM dithiothreitol to determine the Ca/Mg-dependent (primarily DNase I) endonuclease activity or 5 mM MnCl2 , 10 mM Tris-HCl (pH 7.4), and 0.5 mM dithiothreitol for the Mn-dependent (mainly EndoG) activity. .. As opposed to PIA, zymogram gel electrophoresis, previously used by us to assess DNase I activity (Basnakian et al ., ), was not applicable for EndoG due to low specific activity of the enzyme in the used tubular epithelial cells.

    Article Title: Genetic Variation in Sialidase and Linkage to N-acetylneuraminate Catabolism in Mycoplasma synoviae
    Article Snippet: .. Genomic DNA from the strains with the highest (WVU1853T ) and lowest (K4907A and K5395B) levels of sialidase activity was digested with endonuclease Vsp I (New England Biolabs, Ipswich, Massachusetts), then separated on a 0.6% agarose gel. ..

    Article Title: Digital MDA for enumeration of total nucleic acid contamination
    Article Snippet: .. Endonuclease activity was tested by incubation of enzyme samples with 1 μg ϕ × 174 virion (ss, closed circular) DNA (New England Biolabs) at 37°C for 4 h in DNAse I reaction buffer (New England Biolabs). .. DNA shearing Twenty nanograms E. coli K12 genomic DNA [again prepared by the DNeasy method (Qiagen) for Gram-negative cells] was diluted in 200 μl 20 mM Tris, pH 7.5, containing 0.05% Tween-20.

    Article Title: Uptake of Foreign Nucleic Acids in Kidney Tubular Epithelial Cells Deficient in Proapoptotic Endonucleases
    Article Snippet: .. Endonuclease activity in total protein extracts from kidney cells and in culture medium was determined using the plasmid incision assay (PIA) with pBR322 plasmid (New England Biolabs, Beverly, MA) as the substrate as described previously (Basnakian et al ., ). .. To determine whether Lipofectamine is protecting plasmid DNA from degradation by endonucleases, pBR322 plasmid was pretreated with Lipofectamine before it was exposed to the culture medium.

    Article Title: Small-Molecule Antioxidant Proteome-Shields in Deinococcus radiodurans
    Article Snippet: .. Following irradiation, 20 µl of each ionizing radiation-treated Bam HI sample were assayed for residual endonuclease activity in separate reaction mixtures (final volume, 30 µl) containing 125 ng μ-phage DNA, 50 mM NaCl, 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2 , and 1 mM dithiothreitol (New England Biolabs). .. Bam HI/μ DNA mixtures were incubated for 1.25 h at 37°C, followed by agarose (0.8%) gel electrophoresis (AGE).

    Article Title: Tear lipocalin is the major endonuclease in tears
    Article Snippet: .. Endonuclease activity assay In general, DNA-hydrolyzing activity was determined in 20 mM Tris-HCl, pH 7.5, 1 mM MgCl2 , 1mM CaCl2, 50 mM NaCl, and 0.1 μg sc pUC19 plasmid DNA (New England Biolabs, Beverly, MA) in a volume of 20 μl. .. For experiments investigating activity with varying ions, the other components of the assay buffer are specifically indicated.

    Expressing:

    Article Title: Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons
    Article Snippet: Using standard cloning techniques and endonuclease (5′, EcoRI; 3′, Hin dIII) sequence-tagged primers, Sync1-Sync3, Per45 and Per58 were subcloned from pcDNA3 into pMAL (New England Biolabs) using standard methodologies. .. Expression and purification of maltose-binding protein (MPB)-tagged proteins were performed using the pMAL protein fusion and purification system, in accordance with the manufacturer's instructions (New England Biolabs).

    Modification:

    Article Title: Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
    Article Snippet: To study the epigenetic changes in specific DNA sequences it was used MS-RAPD, a modification of the original RAPD technique which is closer to MSAP (methylation sensitive amplified polymorphisms) analysis. .. In the first reaction, 250 ηg of each extracted DNA was added to a restriction mixture containing: 1× restriction buffer and 10/20 U restriction endonuclease to a final volume of 30 µL, one mixture per endonuclease (Hpa II/Msp I; all endonucleases and buffers were supplied by New England Biolabs, USA).

    Hybridization:

    Article Title: Genetic Variation in Sialidase and Linkage to N-acetylneuraminate Catabolism in Mycoplasma synoviae
    Article Snippet: Genomic DNA from the strains with the highest (WVU1853T ) and lowest (K4907A and K5395B) levels of sialidase activity was digested with endonuclease Vsp I (New England Biolabs, Ipswich, Massachusetts), then separated on a 0.6% agarose gel. .. Hybridization of the nanI probe and detection of the digoxygenin label were carried out using the DIG EasyHyb system (Roche Applied Sciences) according to the manufacturer’s instructions.

    Countercurrent Chromatography:

    Article Title: Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons
    Article Snippet: Using standard cloning techniques and endonuclease (5′, EcoRI; 3′, Hin dIII) sequence-tagged primers, Sync1-Sync3, Per45 and Per58 were subcloned from pcDNA3 into pMAL (New England Biolabs) using standard methodologies. .. Syncoilin N-term (residues 1-157) and rod domains (residues 158-452) were subcloned into pMAL using the following primers: N-termF, TTT GAA TTC ATG GCC AGC CCG GAA CCC CT; N-termR, TTT AAG CTT AGA GGG GAT CCT CCT CGG TGT; RodF, TTT GAA TTC AGC GTG GAG GAC CTG GAG CG; RodR, TTT AAG CTT ATG CAT CAG CCT GTT CCA GAC.

    Chromatography:

    Article Title: Small-Molecule Antioxidant Proteome-Shields in Deinococcus radiodurans
    Article Snippet: The synthetic decapeptide (H-Asp-Glu-His-Gly-Thr-Ala-Val-Met-Leu-Lys-OH) was obtained from Elim Biopharmaceuticals, Inc. Hayward, California, USA, and was authenticated at NHLBI by high performance liquid chromatography-mass spectrometry (HPLC-MS). .. Following irradiation, 20 µl of each ionizing radiation-treated Bam HI sample were assayed for residual endonuclease activity in separate reaction mixtures (final volume, 30 µl) containing 125 ng μ-phage DNA, 50 mM NaCl, 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2 , and 1 mM dithiothreitol (New England Biolabs).

    SYBR Green Assay:

    Article Title: Digital MDA for enumeration of total nucleic acid contamination
    Article Snippet: Reaction master mixes were made up according to the manufacturers’ instructions, omitting any chemical or thermal template denaturation steps and supplementing 0.3% Tween-20 and 1× SYBR GREEN I (Invitrogen), used to follow the reactions in real time during a 16 h, 30°C incubation on the MX3005-P thermocycler (Stratagene). .. Endonuclease activity was tested by incubation of enzyme samples with 1 μg ϕ × 174 virion (ss, closed circular) DNA (New England Biolabs) at 37°C for 4 h in DNAse I reaction buffer (New England Biolabs).

    In Vitro:

    Article Title: Inosine modifications in human tRNAs are incorporated at the precursor tRNA level
    Article Snippet: .. The Endonuclease V (EndoV) assay was performed by incubating 1 μg in vitro transcribed pre-tRNAVal (AAC) (incubated or not with hetADAT) with 1 μl EndoV (New England Biolabs) in a 50-μl reaction containing buffer 4 (New England Biolabs) and DEPC-treated water, overnight at 37°C. .. RNA was then dephosphorylated by addition of 1 μl Calf Intestinal Phosphatase (New England Biolabs) to each EndoV reaction and incubation for 1 h at 37°C.

    Article Title: Small-Molecule Antioxidant Proteome-Shields in Deinococcus radiodurans
    Article Snippet: Assays for enzyme activity and DNA damage The chemical agents identified in the DR-ultrafiltrate ( , , and ; and ) were reconstituted in vitro using reagents from Sigma Chemical Company (St. Louis, Missouri, USA). .. Following irradiation, 20 µl of each ionizing radiation-treated Bam HI sample were assayed for residual endonuclease activity in separate reaction mixtures (final volume, 30 µl) containing 125 ng μ-phage DNA, 50 mM NaCl, 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2 , and 1 mM dithiothreitol (New England Biolabs).

    other:

    Article Title: Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V
    Article Snippet: Endonuclease V treatment Escherichia coli or Thermotoga maritima endonuclease V (EndoV) were obtained from New England Biolabs (Frankfurt am Main, Germany) or Thermo Fisher Scientific (St. Leon-Rot, Germany), respectively.

    DNA Sequencing:

    Article Title: Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)
    Article Snippet: 16S-23S rDNA spacer amplicons were digested with 4 U of each endonuclease (New England BioLabs, Saint-Quentin-en-Yvelines, France) and fractionated by electrophoresis in 2.5% high-resolution agarose gels (MetaPhor; TEBU, Le Perray en Yvelines, France) at 100 V for 3 h. With PCR-ribotyping, all of the strains of R. paucula exhibited a single fragment of between 831 and 947 bp in size, based on the use of DNA Molecular Weight Marker III. .. 16S-23S rDNA spacer amplicons were digested with 4 U of each endonuclease (New England BioLabs, Saint-Quentin-en-Yvelines, France) and fractionated by electrophoresis in 2.5% high-resolution agarose gels (MetaPhor; TEBU, Le Perray en Yvelines, France) at 100 V for 3 h. With PCR-ribotyping, all of the strains of R. paucula exhibited a single fragment of between 831 and 947 bp in size, based on the use of DNA Molecular Weight Marker III.

    Protein Concentration:

    Article Title: A Sequence-Specific Nicking Endonuclease from Streptomyces: Purification, Physical and Catalytic Properties
    Article Snippet: The unit of endonuclease activity was quantified by titrating against known units of commercial Eco RI (New England Biolabs). .. Protein concentration was determined by the Bradford method using BioRad protein assay reagent (BioRad, USA) and BSA as the standard.

    Sequencing:

    Article Title: Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)
    Article Snippet: 16S-23S rDNA spacer amplicons were digested with 4 U of each endonuclease (New England BioLabs, Saint-Quentin-en-Yvelines, France) and fractionated by electrophoresis in 2.5% high-resolution agarose gels (MetaPhor; TEBU, Le Perray en Yvelines, France) at 100 V for 3 h. With PCR-ribotyping, all of the strains of R. paucula exhibited a single fragment of between 831 and 947 bp in size, based on the use of DNA Molecular Weight Marker III. .. 16S-23S rDNA spacer amplicons were digested with 4 U of each endonuclease (New England BioLabs, Saint-Quentin-en-Yvelines, France) and fractionated by electrophoresis in 2.5% high-resolution agarose gels (MetaPhor; TEBU, Le Perray en Yvelines, France) at 100 V for 3 h. With PCR-ribotyping, all of the strains of R. paucula exhibited a single fragment of between 831 and 947 bp in size, based on the use of DNA Molecular Weight Marker III.

    Article Title: Inosine modifications in human tRNAs are incorporated at the precursor tRNA level
    Article Snippet: Samples were sent for sequencing using the same oligos used for the PCR reaction as sequencing primers. .. The Endonuclease V (EndoV) assay was performed by incubating 1 μg in vitro transcribed pre-tRNAVal (AAC) (incubated or not with hetADAT) with 1 μl EndoV (New England Biolabs) in a 50-μl reaction containing buffer 4 (New England Biolabs) and DEPC-treated water, overnight at 37°C.

    Article Title: Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons
    Article Snippet: .. Using standard cloning techniques and endonuclease (5′, EcoRI; 3′, Hin dIII) sequence-tagged primers, Sync1-Sync3, Per45 and Per58 were subcloned from pcDNA3 into pMAL (New England Biolabs) using standard methodologies. .. Syncoilin N-term (residues 1-157) and rod domains (residues 158-452) were subcloned into pMAL using the following primers: N-termF, TTT GAA TTC ATG GCC AGC CCG GAA CCC CT; N-termR, TTT AAG CTT AGA GGG GAT CCT CCT CGG TGT; RodF, TTT GAA TTC AGC GTG GAG GAC CTG GAG CG; RodR, TTT AAG CTT ATG CAT CAG CCT GTT CCA GAC.

    Recombinant:

    Article Title: Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons
    Article Snippet: Using standard cloning techniques and endonuclease (5′, EcoRI; 3′, Hin dIII) sequence-tagged primers, Sync1-Sync3, Per45 and Per58 were subcloned from pcDNA3 into pMAL (New England Biolabs) using standard methodologies. .. 10 μg MBP-tagged recombinant proteins were separated on gels and transferred to nitrocellulose membranes as described above.

    Molecular Weight:

    Article Title: Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)
    Article Snippet: .. 16S-23S rDNA spacer amplicons were digested with 4 U of each endonuclease (New England BioLabs, Saint-Quentin-en-Yvelines, France) and fractionated by electrophoresis in 2.5% high-resolution agarose gels (MetaPhor; TEBU, Le Perray en Yvelines, France) at 100 V for 3 h. With PCR-ribotyping, all of the strains of R. paucula exhibited a single fragment of between 831 and 947 bp in size, based on the use of DNA Molecular Weight Marker III. ..

    Article Title: Digital MDA for enumeration of total nucleic acid contamination
    Article Snippet: The crude products were also analyzed by agarose gel electrophoresis to assess the presence of high molecular weight amplified DNA. .. Endonuclease activity was tested by incubation of enzyme samples with 1 μg ϕ × 174 virion (ss, closed circular) DNA (New England Biolabs) at 37°C for 4 h in DNAse I reaction buffer (New England Biolabs).

    Imaging:

    Article Title: Tear lipocalin is the major endonuclease in tears
    Article Snippet: Endonuclease activity assay In general, DNA-hydrolyzing activity was determined in 20 mM Tris-HCl, pH 7.5, 1 mM MgCl2 , 1mM CaCl2, 50 mM NaCl, and 0.1 μg sc pUC19 plasmid DNA (New England Biolabs, Beverly, MA) in a volume of 20 μl. .. Ethidium-bromide stained gels were photographed and scanned with a densitometer (IS-100 Digital Imaging System; Alpha Innotech Corporation, San Leandro, CA).

    DNA Extraction:

    Article Title: Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
    Article Snippet: Methylation-sensitive random-amplified polymorphic DNA (MS-RAPD) Genomic DNA was extracted from 75 mg of frozen leaves with a plant genomic DNA extraction kit (DNeasy Plant Mini Kit, Qiagen, Germany) according to the manufacturer's instructions. .. In the first reaction, 250 ηg of each extracted DNA was added to a restriction mixture containing: 1× restriction buffer and 10/20 U restriction endonuclease to a final volume of 30 µL, one mixture per endonuclease (Hpa II/Msp I; all endonucleases and buffers were supplied by New England Biolabs, USA).

    High Performance Liquid Chromatography:

    Article Title: Small-Molecule Antioxidant Proteome-Shields in Deinococcus radiodurans
    Article Snippet: The synthetic decapeptide (H-Asp-Glu-His-Gly-Thr-Ala-Val-Met-Leu-Lys-OH) was obtained from Elim Biopharmaceuticals, Inc. Hayward, California, USA, and was authenticated at NHLBI by high performance liquid chromatography-mass spectrometry (HPLC-MS). .. Following irradiation, 20 µl of each ionizing radiation-treated Bam HI sample were assayed for residual endonuclease activity in separate reaction mixtures (final volume, 30 µl) containing 125 ng μ-phage DNA, 50 mM NaCl, 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2 , and 1 mM dithiothreitol (New England Biolabs).

    DNA Cleavage Assay:

    Article Title: A Sequence-Specific Nicking Endonuclease from Streptomyces: Purification, Physical and Catalytic Properties
    Article Snippet: Paragraph title: 2.5. DNA Cleavage Assay ... The unit of endonuclease activity was quantified by titrating against known units of commercial Eco RI (New England Biolabs).

    Methylation:

    Article Title: Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
    Article Snippet: Paragraph title: Methylation-sensitive random-amplified polymorphic DNA (MS-RAPD) ... In the first reaction, 250 ηg of each extracted DNA was added to a restriction mixture containing: 1× restriction buffer and 10/20 U restriction endonuclease to a final volume of 30 µL, one mixture per endonuclease (Hpa II/Msp I; all endonucleases and buffers were supplied by New England Biolabs, USA).

    Multiple Displacement Amplification:

    Article Title: Digital MDA for enumeration of total nucleic acid contamination
    Article Snippet: Activity assays The commercial and high-purity ϕ29 DNAP reagents were tested for activity in 50 μl MDA reactions. .. Endonuclease activity was tested by incubation of enzyme samples with 1 μg ϕ × 174 virion (ss, closed circular) DNA (New England Biolabs) at 37°C for 4 h in DNAse I reaction buffer (New England Biolabs).

    Marker:

    Article Title: Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)
    Article Snippet: .. 16S-23S rDNA spacer amplicons were digested with 4 U of each endonuclease (New England BioLabs, Saint-Quentin-en-Yvelines, France) and fractionated by electrophoresis in 2.5% high-resolution agarose gels (MetaPhor; TEBU, Le Perray en Yvelines, France) at 100 V for 3 h. With PCR-ribotyping, all of the strains of R. paucula exhibited a single fragment of between 831 and 947 bp in size, based on the use of DNA Molecular Weight Marker III. ..

    Article Title: Antibiotic resistance, virulence determinants and production of biogenic amines among enterococci from ovine, feline, canine, porcine and human milk
    Article Snippet: .. Later, a representative of each RAPD profile found in each host was submitted to PFGE genotyping [ ]; for this purpose, chromosomal DNA was digested with the endonuclease Sma I (New England Biolabs, Ipswich, MA) at 37°C for 16 h. Then, electrophoresis was carried out in a CHEF DR-III apparatus (Bio-Rad) for 23 h at 14°C at 6 V/cm with pulses from 5 to 50 s. A standard pattern (Lamda Ladder PFG Marker, New England Biolabs) was included in the gels to compare the digitally normalized PFGE profiles. .. Computer-assisted analysis was performed with the Phoretix 1D Pro software (Nonlinear USA, Inc., Durham, NC).

    Article Title: Staphylococcus epidermidis strains isolated from breast milk of women suffering infectious mastitis: potential virulence traits and resistance to antibiotics
    Article Snippet: .. Chromosomal DNA was digested with the endonuclease Sma I (New England Biolabs, Ipswich, MA) at 37°C for 16 h. Electrophoresis was carried out in a CHEF DR-III apparatus (Bio-Rad Laboratories, Hercules, CA) for 23 h at 14°C at 6 V cm-1 with pulses from 5 to 50 s. A standard pattern (Lamda Ladder PFG Marker, New England Biolabs) was included in the gels to allow comparison of the digitally normalized PFGE profiles. .. Computer-assisted analysis of the gels was performed with the Phoretix 1D Pro software (Nonlinear USA, Inc., Durham, NC).

    Labeling:

    Article Title: Genetic Variation in Sialidase and Linkage to N-acetylneuraminate Catabolism in Mycoplasma synoviae
    Article Snippet: The amplicons were labeled with digoxygenin (DIG Hi prime, Roche Applied Sciences) according to the manufacturer’s instructions. .. Genomic DNA from the strains with the highest (WVU1853T ) and lowest (K4907A and K5395B) levels of sialidase activity was digested with endonuclease Vsp I (New England Biolabs, Ipswich, Massachusetts), then separated on a 0.6% agarose gel.

    Purification:

    Article Title: Inosine modifications in human tRNAs are incorporated at the precursor tRNA level
    Article Snippet: Following the PCR reaction, the two identical PCR reactions were combined and purified using Nucleospin Gel and PCR clean-up kit (Macherey-Nagel; REF number: 740609.250). .. The Endonuclease V (EndoV) assay was performed by incubating 1 μg in vitro transcribed pre-tRNAVal (AAC) (incubated or not with hetADAT) with 1 μl EndoV (New England Biolabs) in a 50-μl reaction containing buffer 4 (New England Biolabs) and DEPC-treated water, overnight at 37°C.

    Article Title: Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons
    Article Snippet: Using standard cloning techniques and endonuclease (5′, EcoRI; 3′, Hin dIII) sequence-tagged primers, Sync1-Sync3, Per45 and Per58 were subcloned from pcDNA3 into pMAL (New England Biolabs) using standard methodologies. .. Expression and purification of maltose-binding protein (MPB)-tagged proteins were performed using the pMAL protein fusion and purification system, in accordance with the manufacturer's instructions (New England Biolabs).

    Polymerase Chain Reaction:

    Article Title: Ralstonia paucula (Formerly CDC Group IV c-2): Unsuccessful Strain Differentiation with PCR-Based Methods, Study of the 16S-23S Spacer of the rRNA Operon, and Comparison with Other Ralstonia Species (R. eutropha, R. pickettii, R. gilardii, and R. solanacearum)
    Article Snippet: .. 16S-23S rDNA spacer amplicons were digested with 4 U of each endonuclease (New England BioLabs, Saint-Quentin-en-Yvelines, France) and fractionated by electrophoresis in 2.5% high-resolution agarose gels (MetaPhor; TEBU, Le Perray en Yvelines, France) at 100 V for 3 h. With PCR-ribotyping, all of the strains of R. paucula exhibited a single fragment of between 831 and 947 bp in size, based on the use of DNA Molecular Weight Marker III. ..

    Article Title: Genetic Variation in Sialidase and Linkage to N-acetylneuraminate Catabolism in Mycoplasma synoviae
    Article Snippet: To compare the nanI copy number among strains by Southern blotting, a probe consisting of the 3′ 2.8 kb of nanI was amplified from WVU1853T genomic DNA using PCR primers 5′-TCT CTT CCT TTT TGA GGG CTA-3′ and 5′-GCA AAT CAT CTT AAG AAA AGT CAT T-3′. .. Genomic DNA from the strains with the highest (WVU1853T ) and lowest (K4907A and K5395B) levels of sialidase activity was digested with endonuclease Vsp I (New England Biolabs, Ipswich, Massachusetts), then separated on a 0.6% agarose gel.

    Article Title: Inosine modifications in human tRNAs are incorporated at the precursor tRNA level
    Article Snippet: Samples were sent for sequencing using the same oligos used for the PCR reaction as sequencing primers. .. The Endonuclease V (EndoV) assay was performed by incubating 1 μg in vitro transcribed pre-tRNAVal (AAC) (incubated or not with hetADAT) with 1 μl EndoV (New England Biolabs) in a 50-μl reaction containing buffer 4 (New England Biolabs) and DEPC-treated water, overnight at 37°C.

    Article Title: Hydroxylation index of omeprazole in relation to CYP2C19 polymorphism and sex in a healthy Iranian population
    Article Snippet: .. The PCR product of each reaction was digested by specific endonuclease (all from New England Biolabs GmbH, Frankfurt, Germany); the 169 bp CYP2C19*2 product was digested by SmaI to 40 and 129 bp fragments. .. The 329 bp PCR product of CYP2C19*3 was digested by BamHI to 233 and 96 bp pieces.

    Article Title: Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
    Article Snippet: In the first reaction, 250 ηg of each extracted DNA was added to a restriction mixture containing: 1× restriction buffer and 10/20 U restriction endonuclease to a final volume of 30 µL, one mixture per endonuclease (Hpa II/Msp I; all endonucleases and buffers were supplied by New England Biolabs, USA). .. PCR amplification was performed according to Cocconcelli et al. : 20 µL reaction mixtures containing: 12–14 ng DNA, 1× PCR buffer (Invitrogen, USA), 3.5 mM MgCl2 , 75 pM dNTP, 0.25 mM each primer, 1 U Taq polymerase (Invitrogen, USA).

    Salting Out:

    Article Title: Hydroxylation index of omeprazole in relation to CYP2C19 polymorphism and sex in a healthy Iranian population
    Article Snippet: CYP2C19 genotyping The DNA was extracted from blood leucocytes by standard salting out method as explained by Miller et al . .. The PCR product of each reaction was digested by specific endonuclease (all from New England Biolabs GmbH, Frankfurt, Germany); the 169 bp CYP2C19*2 product was digested by SmaI to 40 and 129 bp fragments.

    Nested PCR:

    Article Title: Hydroxylation index of omeprazole in relation to CYP2C19 polymorphism and sex in a healthy Iranian population
    Article Snippet: The PCR product of each reaction was digested by specific endonuclease (all from New England Biolabs GmbH, Frankfurt, Germany); the 169 bp CYP2C19*2 product was digested by SmaI to 40 and 129 bp fragments. .. Genotyping of CYP2C19*17 -3402 C > T and −806 C > T polymorphisms was done by PCR-RFLP and nested-PCR assays as defined by Sim et al .

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Genetic Variation in Sialidase and Linkage to N-acetylneuraminate Catabolism in Mycoplasma synoviae
    Article Snippet: To compare the nanI copy number among strains by Southern blotting, a probe consisting of the 3′ 2.8 kb of nanI was amplified from WVU1853T genomic DNA using PCR primers 5′-TCT CTT CCT TTT TGA GGG CTA-3′ and 5′-GCA AAT CAT CTT AAG AAA AGT CAT T-3′. .. Genomic DNA from the strains with the highest (WVU1853T ) and lowest (K4907A and K5395B) levels of sialidase activity was digested with endonuclease Vsp I (New England Biolabs, Ipswich, Massachusetts), then separated on a 0.6% agarose gel.

    Article Title: Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons
    Article Snippet: Using standard cloning techniques and endonuclease (5′, EcoRI; 3′, Hin dIII) sequence-tagged primers, Sync1-Sync3, Per45 and Per58 were subcloned from pcDNA3 into pMAL (New England Biolabs) using standard methodologies. .. Syncoilin N-term (residues 1-157) and rod domains (residues 158-452) were subcloned into pMAL using the following primers: N-termF, TTT GAA TTC ATG GCC AGC CCG GAA CCC CT; N-termR, TTT AAG CTT AGA GGG GAT CCT CCT CGG TGT; RodF, TTT GAA TTC AGC GTG GAG GAC CTG GAG CG; RodR, TTT AAG CTT ATG CAT CAG CCT GTT CCA GAC.

    Plasmid Preparation:

    Article Title: A Sequence-Specific Nicking Endonuclease from Streptomyces: Purification, Physical and Catalytic Properties
    Article Snippet: DNA Cleavage Assay Enzyme activity was measured by incubating the endonuclease with supercoiled plasmid or lambda DNA in buffer R (10 mM Tris-HCl (pH 8.0), 10 mM MgCl2 , 50 mM NaCl, and 5 mM β -mercaptoethanol). .. The unit of endonuclease activity was quantified by titrating against known units of commercial Eco RI (New England Biolabs).

    Article Title: Uptake of Foreign Nucleic Acids in Kidney Tubular Epithelial Cells Deficient in Proapoptotic Endonucleases
    Article Snippet: .. Endonuclease activity was measured the same way as above in samples containing serially diluted protein (1:5), 1 μg plasmid pBR322 DNA (New England Biolabs), 2 mM CaCl2 , 5 mM MgCl2 , 10 mM Tris-HCl, pH 7.4, and 0.5 mM dithiothreitol to determine the Ca/Mg-dependent (primarily DNase I) endonuclease activity or 5 mM MnCl2 , 10 mM Tris-HCl (pH 7.4), and 0.5 mM dithiothreitol for the Mn-dependent (mainly EndoG) activity. .. As opposed to PIA, zymogram gel electrophoresis, previously used by us to assess DNase I activity (Basnakian et al ., ), was not applicable for EndoG due to low specific activity of the enzyme in the used tubular epithelial cells.

    Article Title: Uptake of Foreign Nucleic Acids in Kidney Tubular Epithelial Cells Deficient in Proapoptotic Endonucleases
    Article Snippet: .. Endonuclease activity in total protein extracts from kidney cells and in culture medium was determined using the plasmid incision assay (PIA) with pBR322 plasmid (New England Biolabs, Beverly, MA) as the substrate as described previously (Basnakian et al ., ). .. To determine whether Lipofectamine is protecting plasmid DNA from degradation by endonucleases, pBR322 plasmid was pretreated with Lipofectamine before it was exposed to the culture medium.

    Article Title: Tear lipocalin is the major endonuclease in tears
    Article Snippet: .. Endonuclease activity assay In general, DNA-hydrolyzing activity was determined in 20 mM Tris-HCl, pH 7.5, 1 mM MgCl2 , 1mM CaCl2, 50 mM NaCl, and 0.1 μg sc pUC19 plasmid DNA (New England Biolabs, Beverly, MA) in a volume of 20 μl. .. For experiments investigating activity with varying ions, the other components of the assay buffer are specifically indicated.

    Software:

    Article Title: Antibiotic resistance, virulence determinants and production of biogenic amines among enterococci from ovine, feline, canine, porcine and human milk
    Article Snippet: Later, a representative of each RAPD profile found in each host was submitted to PFGE genotyping [ ]; for this purpose, chromosomal DNA was digested with the endonuclease Sma I (New England Biolabs, Ipswich, MA) at 37°C for 16 h. Then, electrophoresis was carried out in a CHEF DR-III apparatus (Bio-Rad) for 23 h at 14°C at 6 V/cm with pulses from 5 to 50 s. A standard pattern (Lamda Ladder PFG Marker, New England Biolabs) was included in the gels to compare the digitally normalized PFGE profiles. .. Computer-assisted analysis was performed with the Phoretix 1D Pro software (Nonlinear USA, Inc., Durham, NC).

    Article Title: Staphylococcus epidermidis strains isolated from breast milk of women suffering infectious mastitis: potential virulence traits and resistance to antibiotics
    Article Snippet: Chromosomal DNA was digested with the endonuclease Sma I (New England Biolabs, Ipswich, MA) at 37°C for 16 h. Electrophoresis was carried out in a CHEF DR-III apparatus (Bio-Rad Laboratories, Hercules, CA) for 23 h at 14°C at 6 V cm-1 with pulses from 5 to 50 s. A standard pattern (Lamda Ladder PFG Marker, New England Biolabs) was included in the gels to allow comparison of the digitally normalized PFGE profiles. .. Computer-assisted analysis of the gels was performed with the Phoretix 1D Pro software (Nonlinear USA, Inc., Durham, NC).

    Irradiation:

    Article Title: Small-Molecule Antioxidant Proteome-Shields in Deinococcus radiodurans
    Article Snippet: .. Following irradiation, 20 µl of each ionizing radiation-treated Bam HI sample were assayed for residual endonuclease activity in separate reaction mixtures (final volume, 30 µl) containing 125 ng μ-phage DNA, 50 mM NaCl, 10 mM Tris-HCl (pH 7.9), 10 mM MgCl2 , and 1 mM dithiothreitol (New England Biolabs). .. Bam HI/μ DNA mixtures were incubated for 1.25 h at 37°C, followed by agarose (0.8%) gel electrophoresis (AGE).

    Agarose Gel Electrophoresis:

    Article Title: Uptake of Foreign Nucleic Acids in Kidney Tubular Epithelial Cells Deficient in Proapoptotic Endonucleases
    Article Snippet: The samples were run on a 1% agarose gel in Tris-acetate-EDTA buffer, pH 8 (7V/cm, 35 min), and the DNA was viewed with ethidium bromide. .. Endonuclease activity was measured the same way as above in samples containing serially diluted protein (1:5), 1 μg plasmid pBR322 DNA (New England Biolabs), 2 mM CaCl2 , 5 mM MgCl2 , 10 mM Tris-HCl, pH 7.4, and 0.5 mM dithiothreitol to determine the Ca/Mg-dependent (primarily DNase I) endonuclease activity or 5 mM MnCl2 , 10 mM Tris-HCl (pH 7.4), and 0.5 mM dithiothreitol for the Mn-dependent (mainly EndoG) activity.

    Article Title: Genetic Variation in Sialidase and Linkage to N-acetylneuraminate Catabolism in Mycoplasma synoviae
    Article Snippet: .. Genomic DNA from the strains with the highest (WVU1853T ) and lowest (K4907A and K5395B) levels of sialidase activity was digested with endonuclease Vsp I (New England Biolabs, Ipswich, Massachusetts), then separated on a 0.6% agarose gel. ..

    Article Title: Digital MDA for enumeration of total nucleic acid contamination
    Article Snippet: The crude products were also analyzed by agarose gel electrophoresis to assess the presence of high molecular weight amplified DNA. .. Endonuclease activity was tested by incubation of enzyme samples with 1 μg ϕ × 174 virion (ss, closed circular) DNA (New England Biolabs) at 37°C for 4 h in DNAse I reaction buffer (New England Biolabs).

    Article Title: Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
    Article Snippet: DNA yield and purity were assessed by spectrophotometry (as described in Valledor et al. ) and gel electrophoresis on agarose gel by direct comparison with phage λ DNA. .. In the first reaction, 250 ηg of each extracted DNA was added to a restriction mixture containing: 1× restriction buffer and 10/20 U restriction endonuclease to a final volume of 30 µL, one mixture per endonuclease (Hpa II/Msp I; all endonucleases and buffers were supplied by New England Biolabs, USA).

    Southern Blot:

    Article Title: Genetic Variation in Sialidase and Linkage to N-acetylneuraminate Catabolism in Mycoplasma synoviae
    Article Snippet: Paragraph title: 4.4. Southern blotting to determine nanI copy number ... Genomic DNA from the strains with the highest (WVU1853T ) and lowest (K4907A and K5395B) levels of sialidase activity was digested with endonuclease Vsp I (New England Biolabs, Ipswich, Massachusetts), then separated on a 0.6% agarose gel.

    Spectrophotometry:

    Article Title: Is the Interplay between Epigenetic Markers Related to the Acclimation of Cork Oak Plants to High Temperatures?
    Article Snippet: DNA yield and purity were assessed by spectrophotometry (as described in Valledor et al. ) and gel electrophoresis on agarose gel by direct comparison with phage λ DNA. .. In the first reaction, 250 ηg of each extracted DNA was added to a restriction mixture containing: 1× restriction buffer and 10/20 U restriction endonuclease to a final volume of 30 µL, one mixture per endonuclease (Hpa II/Msp I; all endonucleases and buffers were supplied by New England Biolabs, USA).

    Pulsed-Field Gel:

    Article Title: Staphylococcus epidermidis strains isolated from breast milk of women suffering infectious mastitis: potential virulence traits and resistance to antibiotics
    Article Snippet: Paragraph title: Genotyping of S. epidermidis isolates by pulsed field gel electrophoresis (PFGE) ... Chromosomal DNA was digested with the endonuclease Sma I (New England Biolabs, Ipswich, MA) at 37°C for 16 h. Electrophoresis was carried out in a CHEF DR-III apparatus (Bio-Rad Laboratories, Hercules, CA) for 23 h at 14°C at 6 V cm-1 with pulses from 5 to 50 s. A standard pattern (Lamda Ladder PFG Marker, New England Biolabs) was included in the gels to allow comparison of the digitally normalized PFGE profiles.

    CTG Assay:

    Article Title: Syncoilin modulates peripherin filament networks and is necessary for large-calibre motor neurons
    Article Snippet: Using standard cloning techniques and endonuclease (5′, EcoRI; 3′, Hin dIII) sequence-tagged primers, Sync1-Sync3, Per45 and Per58 were subcloned from pcDNA3 into pMAL (New England Biolabs) using standard methodologies. .. Syncoilin N-term (residues 1-157) and rod domains (residues 158-452) were subcloned into pMAL using the following primers: N-termF, TTT GAA TTC ATG GCC AGC CCG GAA CCC CT; N-termR, TTT AAG CTT AGA GGG GAT CCT CCT CGG TGT; RodF, TTT GAA TTC AGC GTG GAG GAC CTG GAG CG; RodR, TTT AAG CTT ATG CAT CAG CCT GTT CCA GAC.

    Staining:

    Article Title: Tear lipocalin is the major endonuclease in tears
    Article Snippet: Endonuclease activity assay In general, DNA-hydrolyzing activity was determined in 20 mM Tris-HCl, pH 7.5, 1 mM MgCl2 , 1mM CaCl2, 50 mM NaCl, and 0.1 μg sc pUC19 plasmid DNA (New England Biolabs, Beverly, MA) in a volume of 20 μl. .. Ethidium-bromide stained gels were photographed and scanned with a densitometer (IS-100 Digital Imaging System; Alpha Innotech Corporation, San Leandro, CA).

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    New England Biolabs endonuclease
    Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endonuclease/product/New England Biolabs
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