endonuclease iv  (New England Biolabs)


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    Name:
    Endonuclease IV
    Description:
    Endonuclease IV 5 000 units
    Catalog Number:
    m0304l
    Price:
    300
    Size:
    5 000 units
    Category:
    Other Endonucleases
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    Structured Review

    New England Biolabs endonuclease iv
    Endonuclease IV
    Endonuclease IV 5 000 units
    https://www.bioz.com/result/endonuclease iv/product/New England Biolabs
    Average 90 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    endonuclease iv - by Bioz Stars, 2020-01
    90/100 stars

    Images

    1) Product Images from "Error-Prone Processing of Apurinic/Apyrimidinic (AP) Sites by PolX Underlies a Novel Mechanism That Promotes Adaptive Mutagenesis in Bacillus subtilis"

    Article Title: Error-Prone Processing of Apurinic/Apyrimidinic (AP) Sites by PolX Underlies a Novel Mechanism That Promotes Adaptive Mutagenesis in Bacillus subtilis

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.01681-14

    Alkaline agarose gel electrophoresis analysis of DNA isolated from stationary-phase cells (A and B) or exponential-growth-phase cells (C and D) of strain YB955 and strain YB955 nfo exoA nth with or without treatment with EndoIV. Data represent chromosomal
    Figure Legend Snippet: Alkaline agarose gel electrophoresis analysis of DNA isolated from stationary-phase cells (A and B) or exponential-growth-phase cells (C and D) of strain YB955 and strain YB955 nfo exoA nth with or without treatment with EndoIV. Data represent chromosomal

    Techniques Used: Agarose Gel Electrophoresis, Isolation

    2) Product Images from "Chemical repair activity of free radical scavenger edaravone: reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA"

    Article Title: Chemical repair activity of free radical scavenger edaravone: reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA

    Journal: Journal of Radiation Research

    doi: 10.1093/jrr/rru079

    Ratios of the chemical yields of prompt SSBs and of Nth-, Fpg- and Nfo-sensitive sites obtained in the presence of edaravone at several concentrations ( G ) to the yields obtained without edaravone ( G 0 ).
    Figure Legend Snippet: Ratios of the chemical yields of prompt SSBs and of Nth-, Fpg- and Nfo-sensitive sites obtained in the presence of edaravone at several concentrations ( G ) to the yields obtained without edaravone ( G 0 ).

    Techniques Used:

    3) Product Images from "Interaction of Apurinic/Apyrimidinic Endonucleases Nfo and ExoA with the DNA Integrity Scanning Protein DisA in the Processing of Oxidative DNA Damage during Bacillus subtilis Spore Outgrowth"

    Article Title: Interaction of Apurinic/Apyrimidinic Endonucleases Nfo and ExoA with the DNA Integrity Scanning Protein DisA in the Processing of Oxidative DNA Damage during Bacillus subtilis Spore Outgrowth

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.01259-13

    AGE analysis of DNA isolated from dormant or outgrown spores of different strains and with or without treatment with EndoIV or Fpg. Chromosomal DNAs from dormant spores (DS) or outgrown spores (OG) of the wild-type (A), nfo exoA (B), or nfo exoA disA
    Figure Legend Snippet: AGE analysis of DNA isolated from dormant or outgrown spores of different strains and with or without treatment with EndoIV or Fpg. Chromosomal DNAs from dormant spores (DS) or outgrown spores (OG) of the wild-type (A), nfo exoA (B), or nfo exoA disA

    Techniques Used: Isolation

    Related Articles

    Centrifugation:

    Article Title: Global Transcriptome and Physiological Responses of Acinetobacter oleivorans DR1 Exposed to Distinct Classes of Antibiotics
    Article Snippet: After cell debris was removed by centrifugation (13,000×g , 20 min) at 4°C, supernatant was collected and placed on ice. .. We purchased E. coli uracil-DNA glycosylase (UDG), formamidopyrimidine-DNA glycosylase (Fpg), endonuclease IV, and T4 polynucleotide kinase (New England Biolabs, UK).

    Amplification:

    Article Title: Global Transcriptome and Physiological Responses of Acinetobacter oleivorans DR1 Exposed to Distinct Classes of Antibiotics
    Article Snippet: We purchased E. coli uracil-DNA glycosylase (UDG), formamidopyrimidine-DNA glycosylase (Fpg), endonuclease IV, and T4 polynucleotide kinase (New England Biolabs, UK). .. DNA oligonucleotides containing uracil, tetrahydrofuran (THF), or 8- oxoguanine (8-oxoG) residues were provided by Dr. B. Demple, SUNY- Stony Brook (Stony Brook, USA), and these were amplified by means of PCR performed using 30F-F/30F-R, U30-F/U30-R, OxoG-F/OxoG-R primer pairs ( ).

    Synthesized:

    Article Title: Identification of DNA lesions using a third base pair for amplification and nanopore sequencing
    Article Snippet: Materials UDG, Fpg, hOGG1, endonuclease IV, T4-polynucleotide kinase, Klenow(exo− ) DNA polymerase, T4-DNA ligase and OneTaq DNA polymerase were purchased from New England Biolabs. .. APE 1 (ref. ) and hNEIL1-DNA glycosylase were a kind gift from Professor Sheila S. David. dNaM and d5SICS 2′-deoxynucleotide-5′-triphosphates were synthesized by previously published procedures .

    Enzyme Activity Assay:

    Article Title: DEMETER and REPRESSOR OF SILENCING 1 encode 5-methylcytosine DNA glycosylases
    Article Snippet: Paragraph title: Enzymatic Activity Assay. ... When required, the DNA was resuspended in 10 μl of EF buffer (10 mM Tris·HCl, pH 7.5/1 mM EDTA/50 mM NaCl), incubated with 10 units of endonuclease IV (New England Biolabs) at 37°C for 30 min, and finally extracted and precipitated again as above.

    Autoradiography:

    Article Title: DEMETER and REPRESSOR OF SILENCING 1 encode 5-methylcytosine DNA glycosylases
    Article Snippet: When required, the DNA was resuspended in 10 μl of EF buffer (10 mM Tris·HCl, pH 7.5/1 mM EDTA/50 mM NaCl), incubated with 10 units of endonuclease IV (New England Biolabs) at 37°C for 30 min, and finally extracted and precipitated again as above. .. Reaction products were separated in a 12% denaturing polyacrylamide gel containing 7 M urea, visualized by autoradiography, and quantitated by using a phosphor imager (Bio-Rad).

    Electrophoresis:

    Article Title: Zorbamycin has a different DNA sequence selectivity compared with bleomycin and analogues
    Article Snippet: This is required because of the anomalous migration of 3′-phosphoglycolate ends in capillary electrophoresis that interfere with the determination of the DNA sequence specificity [ ]. .. The 10 μL reaction comprised of the BLM-damaged DNA; 50 mM Tris-HCl, pH 7.9; 100 mM NaCl; 10 mM MgCl2 ; 1 mM DTT; and 5 U endonuclease IV (New England Biolabs).

    Incubation:

    Article Title: A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping
    Article Snippet: .. Incubation of plates with gentle shaking for 15 min at 37°C was followed by addition of 100 μl of an enzyme mixture consisting of 6.65 nM of kUNG (WT or mutant) with an excess (1 μl) of Endonuclease IV (NEB). .. The plates were then loaded onto the plate reader before fluorescence data were collected at 30 s intervals over 35 min, at 37°C.

    Article Title: Robust Detection and Identification of Multiple Oomycetes and Fungi in Environmental Samples by Using a Novel Cleavable Padlock Probe-Based Ligation Detection Assay ▿Robust Detection and Identification of Multiple Oomycetes and Fungi in Environmental Samples by Using a Novel Cleavable Padlock Probe-Based Ligation Detection Assay ▿ †Robust Detection and Identification of Multiple Oomycetes and F
    Article Snippet: .. Next, 10 μl of a cleavage mixture (10 U uracil- N -glycosylase [UNG; Applied Biosystems], 10 U endonuclease IV [New England BioLabs], 2× NEBuffer 3 [New England BioLabs], 2× bovine serum albumin) was added to each reaction mixture and the samples were incubated at 37°C for 1.5 h. Subsequently, 2 μl of 1.1 M NaOH was added and samples were incubated at 95°C for 10 min. ..

    Article Title: DEMETER and REPRESSOR OF SILENCING 1 encode 5-methylcytosine DNA glycosylases
    Article Snippet: .. When required, the DNA was resuspended in 10 μl of EF buffer (10 mM Tris·HCl, pH 7.5/1 mM EDTA/50 mM NaCl), incubated with 10 units of endonuclease IV (New England Biolabs) at 37°C for 30 min, and finally extracted and precipitated again as above. ..

    Activity Assay:

    Article Title: A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping
    Article Snippet: Paragraph title: Uracil-DNA glycosylase activity assays ... Incubation of plates with gentle shaking for 15 min at 37°C was followed by addition of 100 μl of an enzyme mixture consisting of 6.65 nM of kUNG (WT or mutant) with an excess (1 μl) of Endonuclease IV (NEB).

    Modification:

    Article Title: 8-Oxo-7,8-dihydroguanine in DNA does not constitute a barrier to transcription, but is converted into transcription-blocking damage by OGG1
    Article Snippet: Modified plasmids were concentrated by Amicon Ultra Ultracel® 30 centrifugal filters (Millipore, Schwalbach am Taunus, Germany), followed by phenol–chlorophorm extraction and ethanol precipitation. .. Similarly, the combination of Ung and Endonuclease IV (NEB GmbH, Frankfurt am Main, Germany) was used to excise uracils and cleave the resulting apyrimidinic sites (AP sites).

    Chromatography:

    Article Title: Global Transcriptome and Physiological Responses of Acinetobacter oleivorans DR1 Exposed to Distinct Classes of Antibiotics
    Article Snippet: We purchased E. coli uracil-DNA glycosylase (UDG), formamidopyrimidine-DNA glycosylase (Fpg), endonuclease IV, and T4 polynucleotide kinase (New England Biolabs, UK). .. Micro Bio-Spin 30 Chromatography Columns were from Bio-Rad.

    Ligation:

    Article Title: 8-Oxo-7,8-dihydroguanine in DNA does not constitute a barrier to transcription, but is converted into transcription-blocking damage by OGG1
    Article Snippet: Ligation was done with 150 U T4 DNA ligase 1 h at 22°C followed by heat-inactivation of the enzyme for 15 min at 65°C. .. Similarly, the combination of Ung and Endonuclease IV (NEB GmbH, Frankfurt am Main, Germany) was used to excise uracils and cleave the resulting apyrimidinic sites (AP sites).

    Article Title: Robust Detection and Identification of Multiple Oomycetes and Fungi in Environmental Samples by Using a Novel Cleavable Padlock Probe-Based Ligation Detection Assay ▿Robust Detection and Identification of Multiple Oomycetes and Fungi in Environmental Samples by Using a Novel Cleavable Padlock Probe-Based Ligation Detection Assay ▿ †Robust Detection and Identification of Multiple Oomycetes and F
    Article Snippet: After ligation, 30 μl distilled water was added to each reaction mixture and the samples were denatured at 95°C for 10 min, followed by rapid transfer onto ice. .. Next, 10 μl of a cleavage mixture (10 U uracil- N -glycosylase [UNG; Applied Biosystems], 10 U endonuclease IV [New England BioLabs], 2× NEBuffer 3 [New England BioLabs], 2× bovine serum albumin) was added to each reaction mixture and the samples were incubated at 37°C for 1.5 h. Subsequently, 2 μl of 1.1 M NaOH was added and samples were incubated at 95°C for 10 min.

    Förster Resonance Energy Transfer:

    Article Title: A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping
    Article Snippet: Uracil-DNA glycosylase activity was determined via a Förster resonance energy transfer (FRET)-based assay , in real time, with a BMG LABTECH FLUOstar OPTIMA plate reader using 485 nm excitation and 520 nm emission wavelengths. .. The FAM and BHQ1 oligonucleotides were formed into an offset duplex (see sub-section Oligonucleotides) and the corresponding U:G and U:A duplexes were mixed with kUNG and an excess of endonuclease IV (NEB), before recording changes in fluorescence due to the release of a short (low Tm) 5′-FAM ssDNA fragment upon cleavage of uracil by kUNG at 37°C.

    other:

    Article Title: Electrical discharges in water induce spores’ DNA damage
    Article Snippet: Detection of base excisions and pyrimidine dimers For each condition, a plug slice of 4 × 2 mm was equilibrated with 150 μL of the 1x endonuclease buffer, provided by the manufacturer, at 37°C for 30 min. To detect apurinic/apyrimidinic sites and pyrimidine dimers, DNA was digested respectively with 5 U of endonuclease IV or T4 endonuclease V (New England Biolabs, France), in 150 μL of fresh buffer for 1 h at 37°C.

    Article Title: Self-catalyzed Site-specific Depurination of G Residues Mediated by Cruciform Extrusion in Closed Circular DNA Plasmids *
    Article Snippet: Single strand-specific mung bean endonuclease (NEB) was used to detect cruciform extrusion; endonuclease IV (NEB) to detect AP sites.

    Article Title: Electrical discharges in water induce spores’ DNA damage
    Article Snippet: For each condition, a plug slice of 4 × 2 mm was equilibrated with 150 μL of the 1x endonuclease buffer, provided by the manufacturer, at 37°C for 30 min. To detect apurinic/apyrimidinic sites and pyrimidine dimers, DNA was digested respectively with 5 U of endonuclease IV or T4 endonuclease V (New England Biolabs, France), in 150 μL of fresh buffer for 1 h at 37°C.

    Sequencing:

    Article Title: Zorbamycin has a different DNA sequence selectivity compared with bleomycin and analogues
    Article Snippet: This is required because of the anomalous migration of 3′-phosphoglycolate ends in capillary electrophoresis that interfere with the determination of the DNA sequence specificity [ ]. .. The 10 μL reaction comprised of the BLM-damaged DNA; 50 mM Tris-HCl, pH 7.9; 100 mM NaCl; 10 mM MgCl2 ; 1 mM DTT; and 5 U endonuclease IV (New England Biolabs).

    Article Title: High-resolution Digital Mapping of N-Methylpurines in Human Cells Reveals Modulation of Their Induction and Repair by Nearest-neighbor Nucleotides *
    Article Snippet: Paragraph title: Construction and Sequencing of Libraries of DNA Fragments of Interest ... Six μg of genomic DNA of each sample (DMS-treated and untreated) were digested with 12 units of MseI and 3 units of endonuclease IV (New England Biolabs) in 100 μl of NEBuffer 3 (50 m m Tris-HCl, 100 m m NaCl, 10 m m MgCl2 , 1 m m DTT, 100 μg/ml BSA, pH 7.9) overnight.

    Sonication:

    Article Title: Global Transcriptome and Physiological Responses of Acinetobacter oleivorans DR1 Exposed to Distinct Classes of Antibiotics
    Article Snippet: The cell pellet was resuspended in < 5 ml of sonication buffer (50 mM Tris-HCl (pH 8.0), 1 mM EDTA, and 0.1 mM DTT), and cells were lysed by sonification. .. We purchased E. coli uracil-DNA glycosylase (UDG), formamidopyrimidine-DNA glycosylase (Fpg), endonuclease IV, and T4 polynucleotide kinase (New England Biolabs, UK).

    Recombinant:

    Article Title: 8-Oxo-7,8-dihydroguanine in DNA does not constitute a barrier to transcription, but is converted into transcription-blocking damage by OGG1
    Article Snippet: Recombinant Fpg protein ( )—a kind gift from Serge Boiteux—was used to verify the presence of 8-oxo-G by conversion of plasmid DNA to the open circular form as described previously ( ). .. Similarly, the combination of Ung and Endonuclease IV (NEB GmbH, Frankfurt am Main, Germany) was used to excise uracils and cleave the resulting apyrimidinic sites (AP sites).

    Fluorescence:

    Article Title: A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping
    Article Snippet: Fluorescence intensity was measured using detection mode set at Plate Mode Kinetic. .. Incubation of plates with gentle shaking for 15 min at 37°C was followed by addition of 100 μl of an enzyme mixture consisting of 6.65 nM of kUNG (WT or mutant) with an excess (1 μl) of Endonuclease IV (NEB).

    Article Title: A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping
    Article Snippet: .. The FAM and BHQ1 oligonucleotides were formed into an offset duplex (see sub-section Oligonucleotides) and the corresponding U:G and U:A duplexes were mixed with kUNG and an excess of endonuclease IV (NEB), before recording changes in fluorescence due to the release of a short (low Tm) 5′-FAM ssDNA fragment upon cleavage of uracil by kUNG at 37°C. .. Accumulation of the liberated 5′-FAM ssDNA resulted in loss of quenching by BHQ-1 and an increased fluorescence signal due to FAM.

    Mutagenesis:

    Article Title: A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping
    Article Snippet: .. Incubation of plates with gentle shaking for 15 min at 37°C was followed by addition of 100 μl of an enzyme mixture consisting of 6.65 nM of kUNG (WT or mutant) with an excess (1 μl) of Endonuclease IV (NEB). .. The plates were then loaded onto the plate reader before fluorescence data were collected at 30 s intervals over 35 min, at 37°C.

    Polymerase Chain Reaction:

    Article Title: Global Transcriptome and Physiological Responses of Acinetobacter oleivorans DR1 Exposed to Distinct Classes of Antibiotics
    Article Snippet: We purchased E. coli uracil-DNA glycosylase (UDG), formamidopyrimidine-DNA glycosylase (Fpg), endonuclease IV, and T4 polynucleotide kinase (New England Biolabs, UK). .. DNA oligonucleotides containing uracil, tetrahydrofuran (THF), or 8- oxoguanine (8-oxoG) residues were provided by Dr. B. Demple, SUNY- Stony Brook (Stony Brook, USA), and these were amplified by means of PCR performed using 30F-F/30F-R, U30-F/U30-R, OxoG-F/OxoG-R primer pairs ( ).

    Plasmid Preparation:

    Article Title: 8-Oxo-7,8-dihydroguanine in DNA does not constitute a barrier to transcription, but is converted into transcription-blocking damage by OGG1
    Article Snippet: Paragraph title: Plasmid substrates ... Similarly, the combination of Ung and Endonuclease IV (NEB GmbH, Frankfurt am Main, Germany) was used to excise uracils and cleave the resulting apyrimidinic sites (AP sites).

    Software:

    Article Title: 8-Oxo-7,8-dihydroguanine in DNA does not constitute a barrier to transcription, but is converted into transcription-blocking damage by OGG1
    Article Snippet: Similarly, the combination of Ung and Endonuclease IV (NEB GmbH, Frankfurt am Main, Germany) was used to excise uracils and cleave the resulting apyrimidinic sites (AP sites). .. Relative intensities of DNA bands were determined with a GelDoc 1000 and the Molecular Analyst 2.1 software (Bio-Rad Laboratories, München, Germany).

    SYBR Green Assay:

    Article Title: Highly Sensitive Detection of Uracil-DNA Glycosylase Activity Based on Self-Initiating Multiple Rolling Circle Amplification
    Article Snippet: 4.1 Materials and Reagents Uracil-DNA glycosylase (UDG), human alkyladenine glycosylase (hAAG), uracil glycosylase inhibitor (UGI), T4 DNA ligase, exonuclease I (Exo I), exonuclease III (Exo III), endonuclease IV (Endo IV), and 10× NEBuffer 2 (500 mM NaCl, 100 mM Tris-HCl, 100 mM MgCl2 , 10 mM dithiothreitol) were purchased from New England Biolabs (Ipswich, MA, USA). .. 10× SYBR Green I was purchased from Zeesan (Xiamen, China).

    Agarose Gel Electrophoresis:

    Article Title: 8-Oxo-7,8-dihydroguanine in DNA does not constitute a barrier to transcription, but is converted into transcription-blocking damage by OGG1
    Article Snippet: Similarly, the combination of Ung and Endonuclease IV (NEB GmbH, Frankfurt am Main, Germany) was used to excise uracils and cleave the resulting apyrimidinic sites (AP sites). .. Agarose gel electrophoresis was performed in the presence of 0.5 mg/l ethidium bromide.

    In Vitro:

    Article Title: Global Transcriptome and Physiological Responses of Acinetobacter oleivorans DR1 Exposed to Distinct Classes of Antibiotics
    Article Snippet: Paragraph title: In vitro base-excision repair (BER) assay ... We purchased E. coli uracil-DNA glycosylase (UDG), formamidopyrimidine-DNA glycosylase (Fpg), endonuclease IV, and T4 polynucleotide kinase (New England Biolabs, UK).

    Article Title: Excision of 5-hydroxymethylcytosine by DEMETER family DNA glycosylases
    Article Snippet: Paragraph title: 2.6. In vitro nucleotide incorporation assay ... Twenty five nM of radiolabeled oligonucleotide substrate containing 5mC or 5hmC was reacted with 100 nM of MBP-DMEΔ and 10 U of Endonuclease IV (NEB) in the glycosylase reaction buffer at 37 °C for 1 h. Following heat-inactivation at 65 °C for 15 min, the reaction was subjected to nucleotide incorporation with 0.1 mM dCTP using 5 U of Klenow fragment (3′ to 5′ exo-) (NEB) at 25 °C for 15 min.

    Ethanol Precipitation:

    Article Title: Zorbamycin has a different DNA sequence selectivity compared with bleomycin and analogues
    Article Snippet: The 10 μL reaction comprised of the BLM-damaged DNA; 50 mM Tris-HCl, pH 7.9; 100 mM NaCl; 10 mM MgCl2 ; 1 mM DTT; and 5 U endonuclease IV (New England Biolabs). .. The reaction was allowed to proceed at 37°C for 1 h, followed by ethanol precipitation and the pellet was redissolved in 5 μL 10 mM Tris-HCl, pH 8; 0.1 mM EDTA.

    Article Title: 8-Oxo-7,8-dihydroguanine in DNA does not constitute a barrier to transcription, but is converted into transcription-blocking damage by OGG1
    Article Snippet: Modified plasmids were concentrated by Amicon Ultra Ultracel® 30 centrifugal filters (Millipore, Schwalbach am Taunus, Germany), followed by phenol–chlorophorm extraction and ethanol precipitation. .. Similarly, the combination of Ung and Endonuclease IV (NEB GmbH, Frankfurt am Main, Germany) was used to excise uracils and cleave the resulting apyrimidinic sites (AP sites).

    Concentration Assay:

    Article Title: A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping
    Article Snippet: An increasing concentration of duplex DNA (26.6, 40, 53.3, 66.7, 100, 133.3, 200, 266.7 and 400 nM) in 50 μl buffer composed of 25 mM Tris, 100 mM NaCl pH 7.4, 0.1 mg/ml bovine serum albumin, 0.05% Tween-20, 20 mM MgCl2 was added to 96-well black Microfluor 1 flat bottom microtiter plates (Thermo Scientific). .. Incubation of plates with gentle shaking for 15 min at 37°C was followed by addition of 100 μl of an enzyme mixture consisting of 6.65 nM of kUNG (WT or mutant) with an excess (1 μl) of Endonuclease IV (NEB).

    Article Title: High-resolution Digital Mapping of N-Methylpurines in Human Cells Reveals Modulation of Their Induction and Repair by Nearest-neighbor Nucleotides *
    Article Snippet: Six μg of genomic DNA of each sample (DMS-treated and untreated) were digested with 12 units of MseI and 3 units of endonuclease IV (New England Biolabs) in 100 μl of NEBuffer 3 (50 m m Tris-HCl, 100 m m NaCl, 10 m m MgCl2 , 1 m m DTT, 100 μg/ml BSA, pH 7.9) overnight. .. Each of the samples was added with NaCl to a final concentration of 1 m and a mixture (0.5 pmol each) of biotinylated oligonucleotides (see ).

    Migration:

    Article Title: Zorbamycin has a different DNA sequence selectivity compared with bleomycin and analogues
    Article Snippet: This is required because of the anomalous migration of 3′-phosphoglycolate ends in capillary electrophoresis that interfere with the determination of the DNA sequence specificity [ ]. .. The 10 μL reaction comprised of the BLM-damaged DNA; 50 mM Tris-HCl, pH 7.9; 100 mM NaCl; 10 mM MgCl2 ; 1 mM DTT; and 5 U endonuclease IV (New England Biolabs).

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  • 90
    New England Biolabs endonuclease iv
    Endonuclease Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endonuclease iv/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    endonuclease iv - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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