endonuclease iv endoiv  (New England Biolabs)


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  • 95
    Name:
    Endonuclease IV
    Description:
    Endonuclease IV 5 000 units
    Catalog Number:
    m0304l
    Price:
    306
    Size:
    5 000 units
    Category:
    Other Endonucleases
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    Structured Review

    New England Biolabs endonuclease iv endoiv
    Endonuclease IV
    Endonuclease IV 5 000 units
    https://www.bioz.com/result/endonuclease iv endoiv/product/New England Biolabs
    Average 95 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    endonuclease iv endoiv - by Bioz Stars, 2020-09
    95/100 stars

    Images

    1) Product Images from "Interaction of Apurinic/Apyrimidinic Endonucleases Nfo and ExoA with the DNA Integrity Scanning Protein DisA in the Processing of Oxidative DNA Damage during Bacillus subtilis Spore Outgrowth"

    Article Title: Interaction of Apurinic/Apyrimidinic Endonucleases Nfo and ExoA with the DNA Integrity Scanning Protein DisA in the Processing of Oxidative DNA Damage during Bacillus subtilis Spore Outgrowth

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.01259-13

    AGE analysis of DNA isolated from dormant or outgrown spores of different strains and with or without treatment with EndoIV or Fpg. Chromosomal DNAs from dormant spores (DS) or outgrown spores (OG) of the wild-type (A), nfo exoA (B), or nfo exoA disA
    Figure Legend Snippet: AGE analysis of DNA isolated from dormant or outgrown spores of different strains and with or without treatment with EndoIV or Fpg. Chromosomal DNAs from dormant spores (DS) or outgrown spores (OG) of the wild-type (A), nfo exoA (B), or nfo exoA disA

    Techniques Used: Isolation

    2) Product Images from "Error-Prone Processing of Apurinic/Apyrimidinic (AP) Sites by PolX Underlies a Novel Mechanism That Promotes Adaptive Mutagenesis in Bacillus subtilis"

    Article Title: Error-Prone Processing of Apurinic/Apyrimidinic (AP) Sites by PolX Underlies a Novel Mechanism That Promotes Adaptive Mutagenesis in Bacillus subtilis

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.01681-14

    Alkaline agarose gel electrophoresis analysis of DNA isolated from stationary-phase cells (A and B) or exponential-growth-phase cells (C and D) of strain YB955 and strain YB955 nfo exoA nth with or without treatment with EndoIV. Data represent chromosomal
    Figure Legend Snippet: Alkaline agarose gel electrophoresis analysis of DNA isolated from stationary-phase cells (A and B) or exponential-growth-phase cells (C and D) of strain YB955 and strain YB955 nfo exoA nth with or without treatment with EndoIV. Data represent chromosomal

    Techniques Used: Agarose Gel Electrophoresis, Isolation

    3) Product Images from "Chemical repair activity of free radical scavenger edaravone: reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA"

    Article Title: Chemical repair activity of free radical scavenger edaravone: reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA

    Journal: Journal of Radiation Research

    doi: 10.1093/jrr/rru079

    Ratios of the chemical yields of prompt SSBs and of Nth-, Fpg- and Nfo-sensitive sites obtained in the presence of edaravone at several concentrations ( G ) to the yields obtained without edaravone ( G 0 ).
    Figure Legend Snippet: Ratios of the chemical yields of prompt SSBs and of Nth-, Fpg- and Nfo-sensitive sites obtained in the presence of edaravone at several concentrations ( G ) to the yields obtained without edaravone ( G 0 ).

    Techniques Used:

    Related Articles

    Fluorescence:

    Article Title: A structurally conserved motif in γ-herpesvirus uracil-DNA glycosylases elicits duplex nucleotide-flipping
    Article Snippet: .. The FAM and BHQ1 oligonucleotides were formed into an offset duplex (see sub-section Oligonucleotides) and the corresponding U:G and U:A duplexes were mixed with kUNG and an excess of endonuclease IV (NEB), before recording changes in fluorescence due to the release of a short (low Tm) 5′-FAM ssDNA fragment upon cleavage of uracil by kUNG at 37°C. .. Accumulation of the liberated 5′-FAM ssDNA resulted in loss of quenching by BHQ-1 and an increased fluorescence signal due to FAM.

    Isolation:

    Article Title: Interaction of Apurinic/Apyrimidinic Endonucleases Nfo and ExoA with the DNA Integrity Scanning Protein DisA in the Processing of Oxidative DNA Damage during Bacillus subtilis Spore Outgrowth
    Article Snippet: .. Samples of chromosomal DNA isolated from germinated or dormant spores of each strain were incubated with 18 units of endonuclease IV (EndoIV), which cleaves DNA at apurinic/apyrimidinic (AP) sites, or 14 units of formamidopyrimidine-DNA glycosylase (Fpg), which cleaves DNA at 8-oxo-guanine (8-oxo-G) residues, respectively, according to the instructions of the supplier (New England BioLabs). .. Enzyme reactions were done for 3 h, and reaction mixtures containing 3 or 5 μg of DNA were then electrophoresed on a 1% alkaline agarose gel, which was then stained with ethidium bromide, as described previously ( ).

    Labeling:

    Article Title: Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer
    Article Snippet: .. After permeabilization, cells were incubated with uracil DNA glycosylase (UDG) to remove uracil (NEB #M0304S), formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S) to remove Fapy lesions, T4 Pyrimidine dimer glycosylase (T4PDG NEB #M-308S) to remove pyrimidine dimer lesions, endonuclease IV (Endo IV NEB #M0304S) to process oxidative damage, AP sites and modifies 3’ phosphates to 3’ OH, and endonuclease VIII (Endo VIII NEB #M0299S) to remove damaged pyrimidines diluted in 1X Thermpol buffer and incubated at 37°C for 1 h. Damage sites are labeled by DNA polymerase I Klenow large fragment (lacking 5’ to 3’ exonuclease activity) incubated with Digoxigenin-11-dUTP, alkali-labile (Dig) (Sigma-Aldrich #DIUTP-RO) at 37°C for 1 h. The Dig-dUTP is covalently incorporated into the DNA for detection of damage sites. .. Cells were then washed in PBS, blocked using 2% BSA in PBS and Dig was then detected using an anti-Dig antibody (abcam #ab420 clone 21H8) at a dilution of 1:250 in 2% BSA in PBS for 1 h at RT.

    Incubation:

    Article Title: Robust Detection and Identification of Multiple Oomycetes and Fungi in Environmental Samples by Using a Novel Cleavable Padlock Probe-Based Ligation Detection Assay ▿Robust Detection and Identification of Multiple Oomycetes and Fungi in Environmental Samples by Using a Novel Cleavable Padlock Probe-Based Ligation Detection Assay ▿ †Robust Detection and Identification of Multiple Oomycetes and F
    Article Snippet: .. Next, 10 μl of a cleavage mixture (10 U uracil- N -glycosylase [UNG; Applied Biosystems], 10 U endonuclease IV [New England BioLabs], 2× NEBuffer 3 [New England BioLabs], 2× bovine serum albumin) was added to each reaction mixture and the samples were incubated at 37°C for 1.5 h. Subsequently, 2 μl of 1.1 M NaOH was added and samples were incubated at 95°C for 10 min. ..

    Article Title: Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer
    Article Snippet: .. After permeabilization, cells were incubated with uracil DNA glycosylase (UDG) to remove uracil (NEB #M0304S), formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S) to remove Fapy lesions, T4 Pyrimidine dimer glycosylase (T4PDG NEB #M-308S) to remove pyrimidine dimer lesions, endonuclease IV (Endo IV NEB #M0304S) to process oxidative damage, AP sites and modifies 3’ phosphates to 3’ OH, and endonuclease VIII (Endo VIII NEB #M0299S) to remove damaged pyrimidines diluted in 1X Thermpol buffer and incubated at 37°C for 1 h. Damage sites are labeled by DNA polymerase I Klenow large fragment (lacking 5’ to 3’ exonuclease activity) incubated with Digoxigenin-11-dUTP, alkali-labile (Dig) (Sigma-Aldrich #DIUTP-RO) at 37°C for 1 h. The Dig-dUTP is covalently incorporated into the DNA for detection of damage sites. .. Cells were then washed in PBS, blocked using 2% BSA in PBS and Dig was then detected using an anti-Dig antibody (abcam #ab420 clone 21H8) at a dilution of 1:250 in 2% BSA in PBS for 1 h at RT.

    Article Title: Interaction of Apurinic/Apyrimidinic Endonucleases Nfo and ExoA with the DNA Integrity Scanning Protein DisA in the Processing of Oxidative DNA Damage during Bacillus subtilis Spore Outgrowth
    Article Snippet: .. Samples of chromosomal DNA isolated from germinated or dormant spores of each strain were incubated with 18 units of endonuclease IV (EndoIV), which cleaves DNA at apurinic/apyrimidinic (AP) sites, or 14 units of formamidopyrimidine-DNA glycosylase (Fpg), which cleaves DNA at 8-oxo-guanine (8-oxo-G) residues, respectively, according to the instructions of the supplier (New England BioLabs). .. Enzyme reactions were done for 3 h, and reaction mixtures containing 3 or 5 μg of DNA were then electrophoresed on a 1% alkaline agarose gel, which was then stained with ethidium bromide, as described previously ( ).

    other:

    Article Title: Chemical repair activity of free radical scavenger edaravone: reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA
    Article Snippet: Formamidopyrimidine-DNA glycosylase (Fpg), endonuclease III (Nth), endonuclease IV (Nfo), and reaction buffer solutions used for the treatment of these enzymes were purchased from New England BioLabs Inc. (Ipswich, MA).

    Activity Assay:

    Article Title: Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer
    Article Snippet: .. After permeabilization, cells were incubated with uracil DNA glycosylase (UDG) to remove uracil (NEB #M0304S), formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S) to remove Fapy lesions, T4 Pyrimidine dimer glycosylase (T4PDG NEB #M-308S) to remove pyrimidine dimer lesions, endonuclease IV (Endo IV NEB #M0304S) to process oxidative damage, AP sites and modifies 3’ phosphates to 3’ OH, and endonuclease VIII (Endo VIII NEB #M0299S) to remove damaged pyrimidines diluted in 1X Thermpol buffer and incubated at 37°C for 1 h. Damage sites are labeled by DNA polymerase I Klenow large fragment (lacking 5’ to 3’ exonuclease activity) incubated with Digoxigenin-11-dUTP, alkali-labile (Dig) (Sigma-Aldrich #DIUTP-RO) at 37°C for 1 h. The Dig-dUTP is covalently incorporated into the DNA for detection of damage sites. .. Cells were then washed in PBS, blocked using 2% BSA in PBS and Dig was then detected using an anti-Dig antibody (abcam #ab420 clone 21H8) at a dilution of 1:250 in 2% BSA in PBS for 1 h at RT.

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  • 95
    New England Biolabs endonuclease iv endoiv
    AGE analysis of <t>DNA</t> isolated from dormant or outgrown spores of different strains and with or without treatment with <t>EndoIV</t> or Fpg. Chromosomal DNAs from dormant spores (DS) or outgrown spores (OG) of the wild-type (A), nfo exoA (B), or nfo exoA disA
    Endonuclease Iv Endoiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endonuclease iv endoiv/product/New England Biolabs
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    endonuclease iv endoiv - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    AGE analysis of DNA isolated from dormant or outgrown spores of different strains and with or without treatment with EndoIV or Fpg. Chromosomal DNAs from dormant spores (DS) or outgrown spores (OG) of the wild-type (A), nfo exoA (B), or nfo exoA disA

    Journal: Journal of Bacteriology

    Article Title: Interaction of Apurinic/Apyrimidinic Endonucleases Nfo and ExoA with the DNA Integrity Scanning Protein DisA in the Processing of Oxidative DNA Damage during Bacillus subtilis Spore Outgrowth

    doi: 10.1128/JB.01259-13

    Figure Lengend Snippet: AGE analysis of DNA isolated from dormant or outgrown spores of different strains and with or without treatment with EndoIV or Fpg. Chromosomal DNAs from dormant spores (DS) or outgrown spores (OG) of the wild-type (A), nfo exoA (B), or nfo exoA disA

    Article Snippet: Samples of chromosomal DNA isolated from germinated or dormant spores of each strain were incubated with 18 units of endonuclease IV (EndoIV), which cleaves DNA at apurinic/apyrimidinic (AP) sites, or 14 units of formamidopyrimidine-DNA glycosylase (Fpg), which cleaves DNA at 8-oxo-guanine (8-oxo-G) residues, respectively, according to the instructions of the supplier (New England BioLabs).

    Techniques: Isolation

    Alkaline agarose gel electrophoresis analysis of DNA isolated from stationary-phase cells (A and B) or exponential-growth-phase cells (C and D) of strain YB955 and strain YB955 nfo exoA nth with or without treatment with EndoIV. Data represent chromosomal

    Journal: Journal of Bacteriology

    Article Title: Error-Prone Processing of Apurinic/Apyrimidinic (AP) Sites by PolX Underlies a Novel Mechanism That Promotes Adaptive Mutagenesis in Bacillus subtilis

    doi: 10.1128/JB.01681-14

    Figure Lengend Snippet: Alkaline agarose gel electrophoresis analysis of DNA isolated from stationary-phase cells (A and B) or exponential-growth-phase cells (C and D) of strain YB955 and strain YB955 nfo exoA nth with or without treatment with EndoIV. Data represent chromosomal

    Article Snippet: To detect AP sites, DNA was digested with 18 U of endonuclease IV (EndoIV) (New England, BioLabs).

    Techniques: Agarose Gel Electrophoresis, Isolation

    Ratios of the chemical yields of prompt SSBs and of Nth-, Fpg- and Nfo-sensitive sites obtained in the presence of edaravone at several concentrations ( G ) to the yields obtained without edaravone ( G 0 ).

    Journal: Journal of Radiation Research

    Article Title: Chemical repair activity of free radical scavenger edaravone: reduction reactions with dGMP hydroxyl radical adducts and suppression of base lesions and AP sites on irradiated plasmid DNA

    doi: 10.1093/jrr/rru079

    Figure Lengend Snippet: Ratios of the chemical yields of prompt SSBs and of Nth-, Fpg- and Nfo-sensitive sites obtained in the presence of edaravone at several concentrations ( G ) to the yields obtained without edaravone ( G 0 ).

    Article Snippet: Formamidopyrimidine-DNA glycosylase (Fpg), endonuclease III (Nth), endonuclease IV (Nfo), and reaction buffer solutions used for the treatment of these enzymes were purchased from New England BioLabs Inc. (Ipswich, MA).

    Techniques: