dna extract  (New England Biolabs)


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  • 93
    Name:
    Endonuclease VIII
    Description:
    Endonuclease VIII 5 000 units
    Catalog Number:
    m0299l
    Price:
    306
    Size:
    5 000 units
    Category:
    Other Endonucleases
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    Structured Review

    New England Biolabs dna extract
    Endonuclease VIII
    Endonuclease VIII 5 000 units
    https://www.bioz.com/result/dna extract/product/New England Biolabs
    Average 93 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    dna extract - by Bioz Stars, 2020-08
    93/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Population genomics of Mesolithic Scandinavia: Investigating early postglacial migration routes and high-latitude adaptation
    Article Snippet: .. Libraries were built as above, except a DNA repair step in which UDG and endonuclease VIII or USER enzyme (NEB) treatment was included in order to remove deaminated cytosines [ ]. qPCR was performed in order to quantify the number of molecules and the optimal number of PCR cycles prior to amplification for each DNA library. .. Furthermore, this step included extraction blanks, library blanks, and amplification blanks to monitor potential contamination.

    Amplification:

    Article Title: Population genomics of Mesolithic Scandinavia: Investigating early postglacial migration routes and high-latitude adaptation
    Article Snippet: .. Libraries were built as above, except a DNA repair step in which UDG and endonuclease VIII or USER enzyme (NEB) treatment was included in order to remove deaminated cytosines [ ]. qPCR was performed in order to quantify the number of molecules and the optimal number of PCR cycles prior to amplification for each DNA library. .. Furthermore, this step included extraction blanks, library blanks, and amplification blanks to monitor potential contamination.

    Ligation:

    Article Title: Telomere Trimming and DNA Damage as Signatures of High Risk Neuroblastoma
    Article Snippet: .. In some of STELA assays, 50 ng of genomic DNA was digested with 5 U of Endonuclease VIII (NEB) and 4 U of Fpg (NEB) in 1× CutSmart buffer (NEB) at 37 °C for 2 h. Both glycosylases were inactivated at 75 °C for 20 min. Digested DNAs were applied to the ligation reaction for STELA analysis. .. In-Gel Hybridization Analysis of ssDNA on Telomere G- and C-Strand The standard in-gel hybridization analysis was performed using a combination of established protocols with minor modification .

    Generated:

    Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
    Article Snippet: .. Sequencing libraries were generated from each DNA extract following a single-stranded library preparation protocol [ ], which included treatment with uracil-DNA glycosylase (New England Biolabs M0279) and endonuclease VIII (New England Biolabs M0299). .. The Klenow Fragment of DNA polymerase I (Thermo Fisher Scientific EP0051) was used for the fill-in reaction [ ].

    Labeling:

    Article Title: Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer
    Article Snippet: .. After permeabilization, cells were incubated with uracil DNA glycosylase (UDG) to remove uracil (NEB #M0304S), formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S) to remove Fapy lesions, T4 Pyrimidine dimer glycosylase (T4PDG NEB #M-308S) to remove pyrimidine dimer lesions, endonuclease IV (Endo IV NEB #M0304S) to process oxidative damage, AP sites and modifies 3’ phosphates to 3’ OH, and endonuclease VIII (Endo VIII NEB #M0299S) to remove damaged pyrimidines diluted in 1X Thermpol buffer and incubated at 37°C for 1 h. Damage sites are labeled by DNA polymerase I Klenow large fragment (lacking 5’ to 3’ exonuclease activity) incubated with Digoxigenin-11-dUTP, alkali-labile (Dig) (Sigma-Aldrich #DIUTP-RO) at 37°C for 1 h. The Dig-dUTP is covalently incorporated into the DNA for detection of damage sites. .. Cells were then washed in PBS, blocked using 2% BSA in PBS and Dig was then detected using an anti-Dig antibody (abcam #ab420 clone 21H8) at a dilution of 1:250 in 2% BSA in PBS for 1 h at RT.

    Produced:

    Article Title: Removal of deaminated cytosines and detection of in vivo methylation in ancient DNA
    Article Snippet: .. USER enzyme is a proprietary-ratio mixture of UDG and endonuclease VIII (endoVIII) produced by New England Biolabs. ..

    Polymerase Chain Reaction:

    Article Title: Population genomics of Mesolithic Scandinavia: Investigating early postglacial migration routes and high-latitude adaptation
    Article Snippet: .. Libraries were built as above, except a DNA repair step in which UDG and endonuclease VIII or USER enzyme (NEB) treatment was included in order to remove deaminated cytosines [ ]. qPCR was performed in order to quantify the number of molecules and the optimal number of PCR cycles prior to amplification for each DNA library. .. Furthermore, this step included extraction blanks, library blanks, and amplification blanks to monitor potential contamination.

    Article Title: Selective enrichment of damaged DNA molecules for ancient genome sequencing
    Article Snippet: .. After washing the beads with 200 μL wash buffer A and 200 μL wash buffer B (see for wash buffer recipes), they were resuspended in an excision reaction mix comprised of the following components: 34.25 μL water, 5 μL 10× GeneAmp PCR buffer II (Life Technologies), 4 μL 25 mM MgCl2 , 1.25 μL 1% Tween-20, 0.5 μL 25 mM dNTP, 0.75 μL 10 U/μL endonuclease VIII, and 0.25 μL 1 U/μL E. coli UDG (both New England Biolabs). ..

    Incubation:

    Article Title: Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer
    Article Snippet: .. After permeabilization, cells were incubated with uracil DNA glycosylase (UDG) to remove uracil (NEB #M0304S), formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S) to remove Fapy lesions, T4 Pyrimidine dimer glycosylase (T4PDG NEB #M-308S) to remove pyrimidine dimer lesions, endonuclease IV (Endo IV NEB #M0304S) to process oxidative damage, AP sites and modifies 3’ phosphates to 3’ OH, and endonuclease VIII (Endo VIII NEB #M0299S) to remove damaged pyrimidines diluted in 1X Thermpol buffer and incubated at 37°C for 1 h. Damage sites are labeled by DNA polymerase I Klenow large fragment (lacking 5’ to 3’ exonuclease activity) incubated with Digoxigenin-11-dUTP, alkali-labile (Dig) (Sigma-Aldrich #DIUTP-RO) at 37°C for 1 h. The Dig-dUTP is covalently incorporated into the DNA for detection of damage sites. .. Cells were then washed in PBS, blocked using 2% BSA in PBS and Dig was then detected using an anti-Dig antibody (abcam #ab420 clone 21H8) at a dilution of 1:250 in 2% BSA in PBS for 1 h at RT.

    Activity Assay:

    Article Title: Defective base excision repair in the response to DNA damaging agents in triple negative breast cancer
    Article Snippet: .. After permeabilization, cells were incubated with uracil DNA glycosylase (UDG) to remove uracil (NEB #M0304S), formamidopyrimidine [Fapy]-DNA glycosylase (Fapy-DNA glycosylase NEB #M0240S) to remove Fapy lesions, T4 Pyrimidine dimer glycosylase (T4PDG NEB #M-308S) to remove pyrimidine dimer lesions, endonuclease IV (Endo IV NEB #M0304S) to process oxidative damage, AP sites and modifies 3’ phosphates to 3’ OH, and endonuclease VIII (Endo VIII NEB #M0299S) to remove damaged pyrimidines diluted in 1X Thermpol buffer and incubated at 37°C for 1 h. Damage sites are labeled by DNA polymerase I Klenow large fragment (lacking 5’ to 3’ exonuclease activity) incubated with Digoxigenin-11-dUTP, alkali-labile (Dig) (Sigma-Aldrich #DIUTP-RO) at 37°C for 1 h. The Dig-dUTP is covalently incorporated into the DNA for detection of damage sites. .. Cells were then washed in PBS, blocked using 2% BSA in PBS and Dig was then detected using an anti-Dig antibody (abcam #ab420 clone 21H8) at a dilution of 1:250 in 2% BSA in PBS for 1 h at RT.

    Sequencing:

    Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
    Article Snippet: .. Sequencing libraries were generated from each DNA extract following a single-stranded library preparation protocol [ ], which included treatment with uracil-DNA glycosylase (New England Biolabs M0279) and endonuclease VIII (New England Biolabs M0299). .. The Klenow Fragment of DNA polymerase I (Thermo Fisher Scientific EP0051) was used for the fill-in reaction [ ].

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  • 94
    New England Biolabs endonuclease viii
    Endonuclease Viii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endonuclease viii/product/New England Biolabs
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    endonuclease viii - by Bioz Stars, 2020-08
    94/100 stars
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