tth endoiv (New England Biolabs)


Structured Review

Tth Endoiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tth endoiv/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Capillary Electrophoresis-Based Functional Genomics Screening to Discover Novel Archaeal DNA Modifying Enzymes"
Article Title: Capillary Electrophoresis-Based Functional Genomics Screening to Discover Novel Archaeal DNA Modifying Enzymes
Journal: Applied and Environmental Microbiology
doi: 10.1128/AEM.02137-21

Figure Legend Snippet: Evaluating the efficiency of functional genomic screening by identification of a known uracil cleavage enzyme. (A) A 5′-FAM labeled DNA substrate containing a dU was incubated with a control fosmid lysate (neg), UDG, or fosmid lysate followed by Tth EndoIV to cleave AP sites. Examples of fosmid lysates that contained uracil cleavage activities include Well H4, I9, O9, and P16. (B) A 384-well fosmid plate was screened for UDG activity yielding 12 positive fosmid lysate hits. Colored squares represent 14 fosmid wells that contain the UDG gene and green squares confirm that UDG activity was observed, while red squares lacked detectable UDG activity. (C) Fosmids containing UDG activity mapped to a region in the T. kodakarensis genome containing the UDG gene.
Techniques Used: Functional Assay, Labeling, Incubation, Activity Assay
2) Product Images from "Evaluation of an Internally Controlled Multiplex Tth Endonuclease Cleavage Loop-Mediated Isothermal Amplification (TEC-LAMP) Assay for the Detection of Bacterial Meningitis Pathogens"
Article Title: Evaluation of an Internally Controlled Multiplex Tth Endonuclease Cleavage Loop-Mediated Isothermal Amplification (TEC-LAMP) Assay for the Detection of Bacterial Meningitis Pathogens
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms19020524

Figure Legend Snippet: TEC-LAMP mechanism. ( A ) TEC-LAMP oligonucleotide components, Tth endonuclease IV enzyme and dsDNA template with oligonucleotide targets highlighted. ( B ) Temperature enabled dsDNA dissociation followed by primer and TEC primer/probe hybridization to corresponding targets. ( C ) Inner primer strand displacement extension, via Bst polymerase, forms dsDNA. Outer primer strand displacement extension dissociates this newly formed dsDNA, forming inner primer linked ssDNA. ( D ) The complementary sections of this newly formed inner primer linked ssDNA hybridize, forming loop structures. The abasic site of the TEC primer/probe is now in dsDNA form, and thus, cleaved by the Tth endonuclease IV enzyme. TEC primer/probe, inner and outer primers hybridize upstream of the stem loop structures. ( E ): Inner primer strand displacement extension forms dsDNA and displaces the downstream stem loop structures, fully dissociating the TEC primer/probe fluorophore and quencher, producing fluorescence. Outer primer strand displacement extension displaces the newly formed dsDNA, producing inner primer linked ssDNA. ( F ) The complementary sections at each end of the newly formed inner primer linked ssDNA hybridize and form loop structures. These double looped DNA templates are targeted by the TEC primer/probe, inner and loop primers, leading to rapid self-primed exponential amplification with increased cleavage and fluorescence events.
Techniques Used: Hybridization, Fluorescence, Amplification