amplification  (New England Biolabs)


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  • 99
    Name:
    LongAmp Taq 2X Master Mix
    Description:
    LongAmp Taq 2X Master Mix 500 rxns
    Catalog Number:
    m0287l
    Price:
    537
    Category:
    Long distance PCR Kits
    Size:
    500 rxns
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    Structured Review

    New England Biolabs amplification
    LongAmp Taq 2X Master Mix
    LongAmp Taq 2X Master Mix 500 rxns
    https://www.bioz.com/result/amplification/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amplification - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Denaturing CLIP (dCLIP) pipeline identifies discrete RNA footprints on chromatin-associated proteins and reveals that CBX7 targets 3’UTRs to regulate mRNA expression
    Article Snippet: 25 PCR amplification cycles were performed on the resulting cDNA using multiplexed primers with Illumina barcodes – distinct barcode for every cell type. .. Amplification was performed with LongAmp™ Taq 2× Master Mix (NEB). .. Amplified PCR products were subjected to PAGE electrophoresis on 6% TBE-acrylamide gel.

    Article Title: Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
    Article Snippet: Bacterial DNA was purified using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) and used as a PCR template. .. PCR amplification of 16S rRNA genes was conducted using the 16S Barcoding Kit (SQK‐RAB204; Oxford Nanopore Technologies, Oxford, UK) containing the 27F/1492R primer set , and LongAmp™ Taq 2× Master Mix (New England Biolabs, Ipswich, MA, USA). .. Amplification was performed using an Applied Biosystems Veriti™ Thermal Cycler (Thermo Fischer Scientific, Waltham, MA, USA) with the following PCR conditions: initial denaturation at 95 °C for 3 min, 25 cycles of 95 °C for 20 s, 55 °C for 30 s, and 65 °C for 2 min, followed by a final extension at 65 °C for 5 min. To determine the effects of human DNA contamination on 16S rRNA gene amplification, genomic DNA purified from the human monocytic cell line THP‐1 was mixed with E. coli DNA and subjected to PCR.

    Article Title: A comprehensive overview of FCGR3A gene variability by full-length gene sequencing including the identification of V158F polymorphism
    Article Snippet: MinION Nanopore-based sequencing Amplicons obtained from 14 DNA samples from the cohort of individuals present in the institute, were barcoded and sequenced following Oxford Nanopore’s instructions (NSK-LSK208). .. Next, 48 ng of amplicon was barcoded using the PCR barcoding Kit 1 (Oxford Nanopore Technologies, Oxford, UK) and LongAmp Taq2x (New England Biolabs, Massachusetts, USA) followed by purification of the barcoded PCR product using CleanPCR beads and determination of DNA concentration. .. The barcoded DNA samples were pooled to an end volume of 1 µg in 45 µl and an endrepair/dA-tailing was performed (NEBNext Ultra II End-Repair/dA-tailing module, New England Biolabs) followed by a purification step using AMPure XP beads (Beckman Coulter, California, USA).

    Article Title: Loss of Fbxw7 triggers mammary tumorigenesis associated with E2F/c-Myc activation and Trp53 mutation
    Article Snippet: .. After centrifugation, 2 μL of resulting DNA was used in PCR amplification of exons 5–6 or 7–8 using LongAmp Taq mastermix (New England Biolabs, M0287S) and the following primers: Exons 5–6: 5′-CGTTACTCGGCTTGTCCCCGACCT-3′and 5′-CAACTGTCTCTAAGACGCACAAC-3′. ..

    Polymerase Chain Reaction:

    Article Title: Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer
    Article Snippet: Bacterial DNA was purified using DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) and used as a PCR template. .. PCR amplification of 16S rRNA genes was conducted using the 16S Barcoding Kit (SQK‐RAB204; Oxford Nanopore Technologies, Oxford, UK) containing the 27F/1492R primer set , and LongAmp™ Taq 2× Master Mix (New England Biolabs, Ipswich, MA, USA). .. Amplification was performed using an Applied Biosystems Veriti™ Thermal Cycler (Thermo Fischer Scientific, Waltham, MA, USA) with the following PCR conditions: initial denaturation at 95 °C for 3 min, 25 cycles of 95 °C for 20 s, 55 °C for 30 s, and 65 °C for 2 min, followed by a final extension at 65 °C for 5 min. To determine the effects of human DNA contamination on 16S rRNA gene amplification, genomic DNA purified from the human monocytic cell line THP‐1 was mixed with E. coli DNA and subjected to PCR.

    Article Title: A comprehensive overview of FCGR3A gene variability by full-length gene sequencing including the identification of V158F polymorphism
    Article Snippet: MinION Nanopore-based sequencing Amplicons obtained from 14 DNA samples from the cohort of individuals present in the institute, were barcoded and sequenced following Oxford Nanopore’s instructions (NSK-LSK208). .. Next, 48 ng of amplicon was barcoded using the PCR barcoding Kit 1 (Oxford Nanopore Technologies, Oxford, UK) and LongAmp Taq2x (New England Biolabs, Massachusetts, USA) followed by purification of the barcoded PCR product using CleanPCR beads and determination of DNA concentration. .. The barcoded DNA samples were pooled to an end volume of 1 µg in 45 µl and an endrepair/dA-tailing was performed (NEBNext Ultra II End-Repair/dA-tailing module, New England Biolabs) followed by a purification step using AMPure XP beads (Beckman Coulter, California, USA).

    Article Title: Expanding the Geographic Characterisation of Epstein–Barr Virus Variation through Gene-Based Approaches
    Article Snippet: Genes, primer sequences, amplicon length, and other details are listed in . .. PCR reactions were performed using the LongAmp® Taq 2× Master Mix (New England BioLabs) following manufacturer guidelines with an annealing temperature of 61 °C. .. Blanks were added to each PCR reaction batch to insure the absence of contamination.

    Article Title: Loss of Fbxw7 triggers mammary tumorigenesis associated with E2F/c-Myc activation and Trp53 mutation
    Article Snippet: .. After centrifugation, 2 μL of resulting DNA was used in PCR amplification of exons 5–6 or 7–8 using LongAmp Taq mastermix (New England Biolabs, M0287S) and the following primers: Exons 5–6: 5′-CGTTACTCGGCTTGTCCCCGACCT-3′and 5′-CAACTGTCTCTAAGACGCACAAC-3′. ..

    Purification:

    Article Title: A comprehensive overview of FCGR3A gene variability by full-length gene sequencing including the identification of V158F polymorphism
    Article Snippet: MinION Nanopore-based sequencing Amplicons obtained from 14 DNA samples from the cohort of individuals present in the institute, were barcoded and sequenced following Oxford Nanopore’s instructions (NSK-LSK208). .. Next, 48 ng of amplicon was barcoded using the PCR barcoding Kit 1 (Oxford Nanopore Technologies, Oxford, UK) and LongAmp Taq2x (New England Biolabs, Massachusetts, USA) followed by purification of the barcoded PCR product using CleanPCR beads and determination of DNA concentration. .. The barcoded DNA samples were pooled to an end volume of 1 µg in 45 µl and an endrepair/dA-tailing was performed (NEBNext Ultra II End-Repair/dA-tailing module, New England Biolabs) followed by a purification step using AMPure XP beads (Beckman Coulter, California, USA).

    Concentration Assay:

    Article Title: A comprehensive overview of FCGR3A gene variability by full-length gene sequencing including the identification of V158F polymorphism
    Article Snippet: MinION Nanopore-based sequencing Amplicons obtained from 14 DNA samples from the cohort of individuals present in the institute, were barcoded and sequenced following Oxford Nanopore’s instructions (NSK-LSK208). .. Next, 48 ng of amplicon was barcoded using the PCR barcoding Kit 1 (Oxford Nanopore Technologies, Oxford, UK) and LongAmp Taq2x (New England Biolabs, Massachusetts, USA) followed by purification of the barcoded PCR product using CleanPCR beads and determination of DNA concentration. .. The barcoded DNA samples were pooled to an end volume of 1 µg in 45 µl and an endrepair/dA-tailing was performed (NEBNext Ultra II End-Repair/dA-tailing module, New England Biolabs) followed by a purification step using AMPure XP beads (Beckman Coulter, California, USA).

    Centrifugation:

    Article Title: Loss of Fbxw7 triggers mammary tumorigenesis associated with E2F/c-Myc activation and Trp53 mutation
    Article Snippet: .. After centrifugation, 2 μL of resulting DNA was used in PCR amplification of exons 5–6 or 7–8 using LongAmp Taq mastermix (New England Biolabs, M0287S) and the following primers: Exons 5–6: 5′-CGTTACTCGGCTTGTCCCCGACCT-3′and 5′-CAACTGTCTCTAAGACGCACAAC-3′. ..

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  • 95
    New England Biolabs longamp taq
    Taxonomic assignment of the mock community consisting of 10 bacterial species. A mixture of DNA from 10 different bacterial species was analyzed by 16S rRNA amplicon sequencing using MinION™. PCR amplification was performed with <t>LongAmp™</t> <t>Taq</t> or KAPA2G™ polymerase. The samples were analyzed by MinION™ sequencing with different run time conditions, and the percentage of reads mapping to the 10 bacterial species is shown. Expected abundance of individual taxa is based on the genome size and copy number of 16S rRNA genes.
    Longamp Taq, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/longamp taq/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    longamp taq - by Bioz Stars, 2021-03
    95/100 stars
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    Taxonomic assignment of the mock community consisting of 10 bacterial species. A mixture of DNA from 10 different bacterial species was analyzed by 16S rRNA amplicon sequencing using MinION™. PCR amplification was performed with LongAmp™ Taq or KAPA2G™ polymerase. The samples were analyzed by MinION™ sequencing with different run time conditions, and the percentage of reads mapping to the 10 bacterial species is shown. Expected abundance of individual taxa is based on the genome size and copy number of 16S rRNA genes.

    Journal: FEBS Open Bio

    Article Title: Rapid bacterial identification by direct PCR amplification of 16S rRNA genes using the MinION™ nanopore sequencer

    doi: 10.1002/2211-5463.12590

    Figure Lengend Snippet: Taxonomic assignment of the mock community consisting of 10 bacterial species. A mixture of DNA from 10 different bacterial species was analyzed by 16S rRNA amplicon sequencing using MinION™. PCR amplification was performed with LongAmp™ Taq or KAPA2G™ polymerase. The samples were analyzed by MinION™ sequencing with different run time conditions, and the percentage of reads mapping to the 10 bacterial species is shown. Expected abundance of individual taxa is based on the genome size and copy number of 16S rRNA genes.

    Article Snippet: PCR amplification of 16S rRNA genes was conducted using the 16S Barcoding Kit (SQK‐RAB204; Oxford Nanopore Technologies, Oxford, UK) containing the 27F/1492R primer set , and LongAmp™ Taq 2× Master Mix (New England Biolabs, Ipswich, MA, USA).

    Techniques: Amplification, Sequencing, Polymerase Chain Reaction