udg  (New England Biolabs)


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  • 99
    Name:
    Afu UDG
    Description:
    Afu UDG 1 000 units
    Catalog Number:
    m0279l
    Price:
    300
    Size:
    1 000 units
    Category:
    Glycosidases
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    Name:
    Antarctic Thermolabile UDG
    Description:
    Antarctic Thermolabile UDG 500 units
    Catalog Number:
    m0372l
    Price:
    304
    Size:
    500 units
    Category:
    DNA Glycosylases
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    Structured Review

    New England Biolabs udg
    Antarctic Thermolabile UDG
    Antarctic Thermolabile UDG 500 units
    https://www.bioz.com/result/udg/product/New England Biolabs
    Average 99 stars, based on 58 article reviews
    Price from $9.99 to $1999.99
    udg - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Recombinant:

    Article Title:
    Article Snippet: .. A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes. ..

    Article Title:
    Article Snippet: .. 5 μL of recombinant BORF2 was equilibrated with 2 μL of A3 proteins for 15 minutes and added to 3 μL of an oligo master mix containing 1 μL of 10.7 μM fluorescent oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 1.475 μL HED buffer for a total reaction volume of 10 μL, which was incubated at 37 °C for 30 minutes. .. Deaminase activity assay then proceeded as above.

    Fluorescence:

    Article Title:
    Article Snippet: A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes. .. Separated DNA fragments were visualized on a Typhoon FLA-7000 scanner on fluorescence mode (GE Healthcare).

    Ethanol Precipitation:

    Article Title:
    Article Snippet: For abasic site production 500 pmoles of the single-stranded uracil-containing oligo (40mer_U) were incubated with 25 units UDG (NEB, M0280) in a 50 µl reaction volume for 30 min at 37°C. .. After phenol/chloroform extraction and ethanol precipitation the abasic site oligo was hybridized to the complementary strand as described above.

    Purification:

    Article Title:
    Article Snippet: Recombinant purified proteins were mixed with 2x reducing sample buffer (100 mM Trish-HCl pH 6.8, 20% glycerol, 4% SDS, 5% β-mercaptoethanol, 0.05% bromophenol blue), run on 4-20% SDS-PAGE gels, stained with Coomassie stain (40% methanol, 10% acetic acid, 0.1% Coomassie R250), then quantified by densitometric analyses on ImageJ using bovine serum albumin as a standard. .. A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes.

    Article Title:
    Article Snippet: For abasic site production 500 pmoles of the single-stranded uracil-containing oligo (40mer_U) were incubated with 25 units UDG (NEB, M0280) in a 50 µl reaction volume for 30 min at 37°C. .. DNA was purified by phenol/chloroform, ethanol precipitated and resuspended in H2 O supplemented with 40 µM BHT and DFOM for abasic site derivatization as outlined below.

    Protein Purification:

    Article Title:
    Article Snippet: Paragraph title: Protein purification from E. coli and DNA deaminase activity assays. ... A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes.

    Concentration Assay:

    Article Title:
    Article Snippet: 5 μL of recombinant BORF2 was equilibrated with 2 μL of A3 proteins for 15 minutes and added to 3 μL of an oligo master mix containing 1 μL of 10.7 μM fluorescent oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 1.475 μL HED buffer for a total reaction volume of 10 μL, which was incubated at 37 °C for 30 minutes. .. Assuming normal data distributions, one-sample t-tests were performed at each concentration of BORF2 to determine if mean A3Bctd or A3H deaminase activity differed from null hypothesis μ=1 (100% activity) with alternative hypothesis μ < 1, df=2. p-values for A3Bctd are as follows for the following concentrations of BORF2 (p=0.0237 at 43.75 nM; p=0.00206 at 87.5 nM; p=9.56×10−5 at 175 nM; p=2.51×10−6 at 350 nM; p=2.685×10−5 at 700 nM) and p-values for A3H are (p=0.970 at 43.75 nM; p=0.816 at 87.5 nM; p=0.424 at 175 nM; p=0.440 at 350nM; p=0.1575 at 700 nM).

    Incubation:

    Article Title:
    Article Snippet: The supernatant was added to 2 mL of Ni-NTA agarose beads (Qiagen 30230) and incubated at 4 °C for 30 minutes with gentle rocking. .. A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes.

    Article Title:
    Article Snippet: .. For abasic site production 500 pmoles of the single-stranded uracil-containing oligo (40mer_U) were incubated with 25 units UDG (NEB, M0280) in a 50 µl reaction volume for 30 min at 37°C. .. After phenol/chloroform extraction and ethanol precipitation the abasic site oligo was hybridized to the complementary strand as described above.

    Article Title:
    Article Snippet: .. 5 μL of recombinant BORF2 was equilibrated with 2 μL of A3 proteins for 15 minutes and added to 3 μL of an oligo master mix containing 1 μL of 10.7 μM fluorescent oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 1.475 μL HED buffer for a total reaction volume of 10 μL, which was incubated at 37 °C for 30 minutes. .. Deaminase activity assay then proceeded as above.

    Activity Assay:

    Article Title:
    Article Snippet: Paragraph title: Protein purification from E. coli and DNA deaminase activity assays. ... A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes.

    Polyacrylamide Gel Electrophoresis:

    Article Title:
    Article Snippet: A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes. .. The reaction was then mixed with 11 μL 2x formamide buffer (80% formamide, 1x TBE, bromophenol blue, and xylene cyanol) and run on a 15% TBE-urea PAGE gel.

    Modification:

    Article Title:
    Article Snippet: .. A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes. ..

    Polymerase Chain Reaction:

    Article Title:
    Article Snippet: PCR mixture contained 1 ng of DNA and gDNA- and mtDNA-specific primers (mmActB and mmCytB, see for sequences). .. For abasic site production 500 pmoles of the single-stranded uracil-containing oligo (40mer_U) were incubated with 25 units UDG (NEB, M0280) in a 50 µl reaction volume for 30 min at 37°C.

    Sonication:

    Article Title:
    Article Snippet: Cells were incubated on ice for 30 minutes, then lysed by pulse sonication two times for 2 minutes in an ice water bath (Branson Sonifer). .. A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes.

    Recombinase Polymerase Amplification:

    Article Title:
    Article Snippet: The AB template was prepared by inserting an oligonucleotide containing deoxyuracil (5′phos-TCGCACT/ideoxyU/AAGTCC), and after linearization with AhdI, the template was treated with UDG (NEB M0280) in the same buffer for 1 h. In , the undamaged template was prepared by linearizing Maxi prep DNA. .. The final reaction buffer composition (except where indicated) was as follows: 29.2 mM Hepes–KOH (pH 7.6), 217 mM potassium glutamate (except , d; Supplemental Figs. S3, S4F: 117 mM), 0.0117% NP-40-S, 1.17 mM DTT, 11.7 mM Mg(OAc)2 , 0.117 mg/ml BSA, 6.7 mM KCl, 3 mM ATP, 400 μM CTP, GTP, UTP, 30 μM dATP, dCTP, dGTP, dTTP, 33 nM α-[32 P]-dCTP, 12.5 nM Cdt1/Mcm2–7, 7.5 nM Cdc6, 3.3 nM ORC, 8.3 nM DDK, 20 nM S-CDK, 30 nM Dpb11, 210 nM GINS, 40 nM Cdc45, 20 nM Pol ε, 5 nM Mcm10, 20 nM Ctf4, 60 nM RPA, 20 nM Csm3/Tof1, 20 nM Mrc1, 20 nM RFC, 20 nM PCNA, 10 nM TopoI, 20 nM Pol α, 5 nM Pol δ, 25 nM Sld3/7, and 50 nM Sld2.

    Cellular Antioxidant Activity Assay:

    Article Title:
    Article Snippet: .. A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes. ..

    SDS Page:

    Article Title:
    Article Snippet: Recombinant purified proteins were mixed with 2x reducing sample buffer (100 mM Trish-HCl pH 6.8, 20% glycerol, 4% SDS, 5% β-mercaptoethanol, 0.05% bromophenol blue), run on 4-20% SDS-PAGE gels, stained with Coomassie stain (40% methanol, 10% acetic acid, 0.1% Coomassie R250), then quantified by densitometric analyses on ImageJ using bovine serum albumin as a standard. .. A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes.

    Plasmid Preparation:

    Article Title:
    Article Snippet: Plasmids with and without DNA damage were prepared as described previously by insertion of oligonucleotides into cassettes at specific sites either ~ 3 kb (plasmid ZN3) or 4.5 kb (plasmid ZN5Sp) from the origin using the following oligonucleotides: undamaged DNA: 5′-phos-TCAGCACTTAAGTCC; THF: 5′-phos-TCAGCACT-/idSp/-AAGTCC, CPD: 5′-phos-TCAGCAC-/CPD/-AAGTCC. .. The AB template was prepared by inserting an oligonucleotide containing deoxyuracil (5′phos-TCGCACT/ideoxyU/AAGTCC), and after linearization with AhdI, the template was treated with UDG (NEB M0280) in the same buffer for 1 h. In , the undamaged template was prepared by linearizing Maxi prep DNA.

    Staining:

    Article Title:
    Article Snippet: Recombinant purified proteins were mixed with 2x reducing sample buffer (100 mM Trish-HCl pH 6.8, 20% glycerol, 4% SDS, 5% β-mercaptoethanol, 0.05% bromophenol blue), run on 4-20% SDS-PAGE gels, stained with Coomassie stain (40% methanol, 10% acetic acid, 0.1% Coomassie R250), then quantified by densitometric analyses on ImageJ using bovine serum albumin as a standard. .. A3 proteins were titrated to achieve equivalent enzymatic cleavage of a fluorescent oligo substrate (RSH5194 5’-ATT ATT ATT ATT CAA ATG GAT TTA TTT ATT TAT TTA TTT ATT T-fluorescein-3’) by mixing together 1 μL recombinant A3, 1 μL 10.7 μM oligo, 0.5 μL 1 mg/mL RNase, 0.025 μL UDG (NEB M0280), and 7.47 μL modified HED buffer (20 mM HEPES, 50 mM NaCl, 0.1mM EDTA, 0.1 mg/mL BSA, pH 7.4) and incubating at 37 °C for 30 minutes.

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  • 90
    New England Biolabs uracil dna glycosylase udg
    Uracil Dna Glycosylase Udg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/uracil dna glycosylase udg/product/New England Biolabs
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    uracil dna glycosylase udg - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

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