uracil dna glycosylase  (New England Biolabs)


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  • 80
    Name:
    Afu UDG
    Description:
    Afu UDG 1 000 units
    Catalog Number:
    m0279l
    Price:
    300
    Size:
    1 000 units
    Category:
    Glycosidases
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    Structured Review

    New England Biolabs uracil dna glycosylase
    Afu UDG
    Afu UDG 1 000 units
    https://www.bioz.com/result/uracil dna glycosylase/product/New England Biolabs
    Average 80 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    uracil dna glycosylase - by Bioz Stars, 2020-01
    80/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
    Article Snippet: DNA extraction was performed according to Dabney et al. [ ] with reduced bone powder input mass, and reduced centrifugation speed of the binding apparatus at approximately 450×g . .. Sequencing libraries were generated from each DNA extract following a single-stranded library preparation protocol [ ], which included treatment with uracil-DNA glycosylase (New England Biolabs M0279) and endonuclease VIII (New England Biolabs M0299).

    Article Title: Selective enrichment of damaged DNA molecules for ancient genome sequencing
    Article Snippet: Paragraph title: DNA extraction and library preparation ... The first steps of U selection were performed exactly following steps 1–15 of the protocol described in with two modifications: (1) In step 1, endonuclease VIII and Afu UDG were replaced by 0.5 μL of 1 U/μL USER enzyme mix (New England Biolabs) where applicable. (2) In step 8, the volume of streptavidine beads was increased to 100 μL per reaction.

    Centrifugation:

    Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
    Article Snippet: The lower centrifugation speed was chosen based on our previous experience that the binding apparatus can break during centrifugation at higher speeds. .. Sequencing libraries were generated from each DNA extract following a single-stranded library preparation protocol [ ], which included treatment with uracil-DNA glycosylase (New England Biolabs M0279) and endonuclease VIII (New England Biolabs M0299).

    Amplification:

    Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
    Article Snippet: Sequencing libraries were generated from each DNA extract following a single-stranded library preparation protocol [ ], which included treatment with uracil-DNA glycosylase (New England Biolabs M0279) and endonuclease VIII (New England Biolabs M0299). .. A quantitative PCR (qPCR) experiment was carried out using 0.2% of the unamplified library to estimate relative library complexities (Additional file : Table S1), and to determine the optimal number of cycles for subsequent indexing PCR, representing the inflection point of the respective library amplification curves, corrected for reaction volume and template amount. qPCR was performed on a PikoReal 96 Real-Time PCR machine (Thermo Fisher Scientific TCR0096) with 3 replicates for each library, involving an initial 10 min denaturation at 95 °C, followed by 40 cycles of: 15 s at 95 °C, 30 s at 60 °C, and 1 min at 72 °C.

    Ligation:

    Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
    Article Snippet: Sequencing libraries were generated from each DNA extract following a single-stranded library preparation protocol [ ], which included treatment with uracil-DNA glycosylase (New England Biolabs M0279) and endonuclease VIII (New England Biolabs M0299). .. 2.5 U/μL of Circligase II (Biozym 131406) was used and the ligation reaction carried out overnight.

    Generated:

    Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
    Article Snippet: .. Sequencing libraries were generated from each DNA extract following a single-stranded library preparation protocol [ ], which included treatment with uracil-DNA glycosylase (New England Biolabs M0279) and endonuclease VIII (New England Biolabs M0299). .. The Klenow Fragment of DNA polymerase I (Thermo Fisher Scientific EP0051) was used for the fill-in reaction [ ].

    Real-time Polymerase Chain Reaction:

    Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
    Article Snippet: Sequencing libraries were generated from each DNA extract following a single-stranded library preparation protocol [ ], which included treatment with uracil-DNA glycosylase (New England Biolabs M0279) and endonuclease VIII (New England Biolabs M0299). .. A quantitative PCR (qPCR) experiment was carried out using 0.2% of the unamplified library to estimate relative library complexities (Additional file : Table S1), and to determine the optimal number of cycles for subsequent indexing PCR, representing the inflection point of the respective library amplification curves, corrected for reaction volume and template amount. qPCR was performed on a PikoReal 96 Real-Time PCR machine (Thermo Fisher Scientific TCR0096) with 3 replicates for each library, involving an initial 10 min denaturation at 95 °C, followed by 40 cycles of: 15 s at 95 °C, 30 s at 60 °C, and 1 min at 72 °C.

    Sequencing:

    Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
    Article Snippet: .. Sequencing libraries were generated from each DNA extract following a single-stranded library preparation protocol [ ], which included treatment with uracil-DNA glycosylase (New England Biolabs M0279) and endonuclease VIII (New England Biolabs M0299). .. The Klenow Fragment of DNA polymerase I (Thermo Fisher Scientific EP0051) was used for the fill-in reaction [ ].

    Incubation:

    Article Title: Selective enrichment of damaged DNA molecules for ancient genome sequencing
    Article Snippet: The first steps of U selection were performed exactly following steps 1–15 of the protocol described in with two modifications: (1) In step 1, endonuclease VIII and Afu UDG were replaced by 0.5 μL of 1 U/μL USER enzyme mix (New England Biolabs) where applicable. (2) In step 8, the volume of streptavidine beads was increased to 100 μL per reaction. .. The beads were then resuspended in the blunt-end reaction mix, incubated for 15 min at 25°C, and washed exactly as described in step 19 of .

    Selection:

    Article Title: Selective enrichment of damaged DNA molecules for ancient genome sequencing
    Article Snippet: .. The first steps of U selection were performed exactly following steps 1–15 of the protocol described in with two modifications: (1) In step 1, endonuclease VIII and Afu UDG were replaced by 0.5 μL of 1 U/μL USER enzyme mix (New England Biolabs) where applicable. (2) In step 8, the volume of streptavidine beads was increased to 100 μL per reaction. .. U selection was then continued as follows: A blunt-end reaction mix was prepared by combining 83.1 μL water, 10 μL 10× Tango buffer (Thermo Scientific), 2.5 μL 1% Tween 20, 0.4 μL 25 mM dNTP, 1 μL 100 mM ATP, 1 μL 5 U/μL T4 DNA polymerase and 2 μL 10 U/μL T4 polynucleotide kinase (both Thermo Scientific).

    Polymerase Chain Reaction:

    Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
    Article Snippet: Sequencing libraries were generated from each DNA extract following a single-stranded library preparation protocol [ ], which included treatment with uracil-DNA glycosylase (New England Biolabs M0279) and endonuclease VIII (New England Biolabs M0299). .. A quantitative PCR (qPCR) experiment was carried out using 0.2% of the unamplified library to estimate relative library complexities (Additional file : Table S1), and to determine the optimal number of cycles for subsequent indexing PCR, representing the inflection point of the respective library amplification curves, corrected for reaction volume and template amount. qPCR was performed on a PikoReal 96 Real-Time PCR machine (Thermo Fisher Scientific TCR0096) with 3 replicates for each library, involving an initial 10 min denaturation at 95 °C, followed by 40 cycles of: 15 s at 95 °C, 30 s at 60 °C, and 1 min at 72 °C.

    Binding Assay:

    Article Title: Reduction of the contaminant fraction of DNA obtained from an ancient giant panda bone
    Article Snippet: The lower centrifugation speed was chosen based on our previous experience that the binding apparatus can break during centrifugation at higher speeds. .. Sequencing libraries were generated from each DNA extract following a single-stranded library preparation protocol [ ], which included treatment with uracil-DNA glycosylase (New England Biolabs M0279) and endonuclease VIII (New England Biolabs M0299).

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  • 80
    New England Biolabs afu udg
    Afu Udg, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/afu udg/product/New England Biolabs
    Average 80 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    afu udg - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

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