taq  (New England Biolabs)


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  • 90
    Name:
    Taq DNA Polymerase with Standard Taq Buffer
    Description:
    Taq DNA Polymerase with Standard Taq Buffer 20 000 units
    Catalog Number:
    m0273e
    Price:
    1200
    Size:
    20 000 units
    Category:
    Thermostable DNA Polymerases
    Buy from Supplier


    Structured Review

    New England Biolabs taq
    Taq DNA Polymerase with Standard Taq Buffer
    Taq DNA Polymerase with Standard Taq Buffer 20 000 units
    https://www.bioz.com/result/taq/product/New England Biolabs
    Average 90 stars, based on 164 article reviews
    Price from $9.99 to $1999.99
    taq - by Bioz Stars, 2020-01
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells
    Article Snippet: .. General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5® High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. PCR amplified or restricted DNA fragments were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co, #740609).

    Article Title: Use of FabV-Triclosan Plasmid Selection System for Efficient Expression and Production of Recombinant Proteins in Escherichia coli
    Article Snippet: .. General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5 High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. PCR amplified or restricted DNA fragments were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co, #740609).

    Article Title: Examining Sources of Error in PCR by Single-Molecule Sequencing
    Article Snippet: Q5 High Fidelity DNA Polymerase (#M0491), Phusion High Fidelity DNA Polymerase (#M0530), Deep Vent DNA Polymerase (#M0258), and Taq DNA polymerase (#M0273) are supplied by New England Biolabs. .. Other polymerases were purchased from: Cloned Pfu DNA Polymerase (Agilent Technologies, #600153), Kapa HiFi HotStart ReadyMix (Kapa Biosystems, #KK2602), KOD DNA Polymerase (Novagen, EMD Millipore # 71085–3), PrimeSTAR GXL DNA Polymerase (Clontech, Takara #R050A).

    Amplification:

    Article Title: Characterization and Mechanism of Stress-induced Translocation of 78-Kilodalton Glucose-regulated Protein (GRP78) to the Cell Surface *
    Article Snippet: .. The construction of expression plasmid for GRP78 substrate binding domain (SBD) with KDEL motif at the C terminus was generated by PCR amplification from FLAG-GRP78 (WT) expression plasmid using TaqDNA polymerase (M0273S, New England Biolabs, Ipswich, MA) and primers 5′-TATTATCCCGGGGTCCAGGCTGGTGTGCTCTCTG-3′ and 5′-TTATATGTCGACCTATTACAACTCATCTTTGGTGACTTCAATCTGTGGGAC-3′. .. The PCR product was inserted in-frame into pDisplay expression vector (Life Technologies) between XmaI and SalI sites.

    Article Title: Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells
    Article Snippet: General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5® High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. PCR amplified or restricted DNA fragments were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co, #740609).

    Article Title: Use of FabV-Triclosan Plasmid Selection System for Efficient Expression and Production of Recombinant Proteins in Escherichia coli
    Article Snippet: General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5 High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. PCR amplified or restricted DNA fragments were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co, #740609).

    Article Title: ATG-18 and EPG-6 are Both Required for Autophagy but Differentially Contribute to Lifespan Control in Caenorhabditis elegans
    Article Snippet: For one PCR reaction, 25 units of Taq DNA polymerase (NEB, M0273) with standard Taq buffer (NEB, M0273L), 200 μM dNTP (NEB, N0446S), 0.5 μM of both primers (Sigma–Aldrich, Munich, Germany), and 5 μL DNA was used. .. For genotyping of epg-6(bp242) tetra-primer amplification-refractory mutation system PCR (tetra-primer ARMS-PCR) was applied [ ].

    Article Title: Caloric restriction engages hepatic RNA processing mechanisms in rhesus monkeys
    Article Snippet: The cDNA was diluted 1:4 with water, and 3 μL of diluted cDNA was used as the template for PCR (New England Biolabs #M0273S), which was run according to the manufacturer’s specifications. .. Following PCR amplification, products were analyzed via a 1.3% agarose gel (containing 83.3 ng/mL ethidium bromide), which was visualized using a UV transilluminator.

    Article Title: Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications
    Article Snippet: For all PCR experiments, post-PCR DNA melt curve analysis was performed to assess amplification specificity. .. To test if different enzymes may affect the relative performance of EG and SG differently, the above experiments (except for the comparison experiment with Power SYBR kit) were repeated with Taq from NEB (Catalog # M0273S), Taq from Promega (Catalog # M1661), Taq from Fermentas(Catalog # EP0401) and home-made Taq.

    Article Title: Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth
    Article Snippet: The presence of L. johnsonii La1 prophage, as previously described (Ventura et al., ; Denou et al., ), was checked by PCR using two primer pairs: 1DF/1B2R resulting in a 1500 bp amplicon for L. johnsonii “La1-like” (with the prophage) and 1EF/1B2R resulting in a 268 bp amplicon as a positive control for L. johnsonii strains. .. PCR reactions were run in a final volume of 25 μl containing 200 ng of bacterial DNA extracted with a standard protocol (Zhong et al., ), 200 nM of each primer (1DF: AGA CTT TTT GGC AGG CAA AGG, 1EF: ACA AAC CAC CAG TGC CTA AGG, 1B2R: GCT CTT CGA GAT CAC TGG GC), 200 nM of dNTP, and 1U of Taq DNA polymerase (NEB M0273S).

    Article Title: One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
    Article Snippet: Single-cell variable regions were amplified by seminested PCR using 5′-ATGGGtTGGTCCTGCATTATACTGT-3′ (forward L-VH recoded) with 5′-GGAAGGTGTGCACACCGCTGGAC-3′ (reverse IgG1) and 5′-AGGGGGCTCTCGCAGGAGACGAGG-3′ (reverse IgM) for the first PCR and forward and 5′-GCTCAGGGAAATARCCCTTGAC-3′ (IgG1 internal reverse) for the second PCR. .. PCRs were performed with Taq polymerase (M0273; NEB).

    Article Title: Examining Sources of Error in PCR by Single-Molecule Sequencing
    Article Snippet: PCR Each amplification reaction was set up according to the manufacturer’s suggested protocols. .. Q5 High Fidelity DNA Polymerase (#M0491), Phusion High Fidelity DNA Polymerase (#M0530), Deep Vent DNA Polymerase (#M0258), and Taq DNA polymerase (#M0273) are supplied by New England Biolabs.

    Positive Control:

    Article Title: Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth
    Article Snippet: The presence of L. johnsonii La1 prophage, as previously described (Ventura et al., ; Denou et al., ), was checked by PCR using two primer pairs: 1DF/1B2R resulting in a 1500 bp amplicon for L. johnsonii “La1-like” (with the prophage) and 1EF/1B2R resulting in a 268 bp amplicon as a positive control for L. johnsonii strains. .. PCR reactions were run in a final volume of 25 μl containing 200 ng of bacterial DNA extracted with a standard protocol (Zhong et al., ), 200 nM of each primer (1DF: AGA CTT TTT GGC AGG CAA AGG, 1EF: ACA AAC CAC CAG TGC CTA AGG, 1B2R: GCT CTT CGA GAT CAC TGG GC), 200 nM of dNTP, and 1U of Taq DNA polymerase (NEB M0273S).

    Polymerase Chain Reaction:

    Article Title: Characterization and Mechanism of Stress-induced Translocation of 78-Kilodalton Glucose-regulated Protein (GRP78) to the Cell Surface *
    Article Snippet: .. The construction of expression plasmid for GRP78 substrate binding domain (SBD) with KDEL motif at the C terminus was generated by PCR amplification from FLAG-GRP78 (WT) expression plasmid using TaqDNA polymerase (M0273S, New England Biolabs, Ipswich, MA) and primers 5′-TATTATCCCGGGGTCCAGGCTGGTGTGCTCTCTG-3′ and 5′-TTATATGTCGACCTATTACAACTCATCTTTGGTGACTTCAATCTGTGGGAC-3′. .. The PCR product was inserted in-frame into pDisplay expression vector (Life Technologies) between XmaI and SalI sites.

    Article Title: Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells
    Article Snippet: .. General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5® High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. PCR amplified or restricted DNA fragments were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co, #740609).

    Article Title: Use of FabV-Triclosan Plasmid Selection System for Efficient Expression and Production of Recombinant Proteins in Escherichia coli
    Article Snippet: .. General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5 High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. PCR amplified or restricted DNA fragments were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co, #740609).

    Article Title: ATG-18 and EPG-6 are Both Required for Autophagy but Differentially Contribute to Lifespan Control in Caenorhabditis elegans
    Article Snippet: .. For one PCR reaction, 25 units of Taq DNA polymerase (NEB, M0273) with standard Taq buffer (NEB, M0273L), 200 μM dNTP (NEB, N0446S), 0.5 μM of both primers (Sigma–Aldrich, Munich, Germany), and 5 μL DNA was used. .. For genotyping of epg-6(bp242) tetra-primer amplification-refractory mutation system PCR (tetra-primer ARMS-PCR) was applied [ ].

    Article Title: Caloric restriction engages hepatic RNA processing mechanisms in rhesus monkeys
    Article Snippet: .. The cDNA was diluted 1:4 with water, and 3 μL of diluted cDNA was used as the template for PCR (New England Biolabs #M0273S), which was run according to the manufacturer’s specifications. .. Following PCR amplification, products were analyzed via a 1.3% agarose gel (containing 83.3 ng/mL ethidium bromide), which was visualized using a UV transilluminator.

    Article Title: Combined magnetic tweezers and micro-mirror total internal reflection fluorescence microscope for single molecule manipulation and visualization
    Article Snippet: .. Taq DNA polymerase (M0273, NEB) and supplied buffer 100 μM Deoxynucleotide (dNTP) Solution Biotin-16-dUTP (11093070910, Roche) Digoxigenin-11-dUTP (11093088910, Roche) DNA purification kit (QIAquick PCR purification kit, Qiagen) .. BsaI-HF (R3535, NEB) and supplied buffer Products from PCR reactions DNA clean-up kit (11732668001, Roche)

    Article Title: Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications
    Article Snippet: For all PCR experiments, post-PCR DNA melt curve analysis was performed to assess amplification specificity. .. To test if different enzymes may affect the relative performance of EG and SG differently, the above experiments (except for the comparison experiment with Power SYBR kit) were repeated with Taq from NEB (Catalog # M0273S), Taq from Promega (Catalog # M1661), Taq from Fermentas(Catalog # EP0401) and home-made Taq.

    Article Title: Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth
    Article Snippet: .. PCR reactions were run in a final volume of 25 μl containing 200 ng of bacterial DNA extracted with a standard protocol (Zhong et al., ), 200 nM of each primer (1DF: AGA CTT TTT GGC AGG CAA AGG, 1EF: ACA AAC CAC CAG TGC CTA AGG, 1B2R: GCT CTT CGA GAT CAC TGG GC), 200 nM of dNTP, and 1U of Taq DNA polymerase (NEB M0273S). .. Reactions were initiated with an initial denaturation for 5 min at 95°C followed by 35 cycles at 95°C for 30 s, 50°C for 30 s and 72°C for 1 min. PCR products were visualized on 0.8% agarose gel containing 0.5 ng/μl of BET and images were captured with a camera coupled with Biocapt software, Vilber Lourmat.

    Article Title: Cystathionine ?-lyase: clinical, metabolic, genetic, and structural studies
    Article Snippet: .. PCR was performed using either PfuTurbo DNA polymerase (Stratagene 600250, Cedar Creek, TX) or Taq DNA polymerase (New England Biolabs M0273S, Ipswich, MA). .. The PCR conditions used were 2 minutes at 94°C 1 cycle; 30 seconds at 94°C, 30 seconds at 52°C, 2 minutes at 72°C 35 cycles; 7 minutes at 72°C 1 cycle in the Stratagene Robocycler.

    Article Title: One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
    Article Snippet: Paragraph title: Single-cell Igh and Igk PCR and sequence analysis ... PCRs were performed with Taq polymerase (M0273; NEB).

    Article Title: Examining Sources of Error in PCR by Single-Molecule Sequencing
    Article Snippet: Paragraph title: PCR ... Q5 High Fidelity DNA Polymerase (#M0491), Phusion High Fidelity DNA Polymerase (#M0530), Deep Vent DNA Polymerase (#M0258), and Taq DNA polymerase (#M0273) are supplied by New England Biolabs.

    Article Title: Mechanical Disruption of Lysis-Resistant Bacterial Cells by Use of a Miniature, Low-Power, Disposable Device ▿Mechanical Disruption of Lysis-Resistant Bacterial Cells by Use of a Miniature, Low-Power, Disposable Device ▿ †
    Article Snippet: Standard Taq buffer (B9014S), Taq DNA polymerase (M0273L), and deoxynucleotide triphosphate (dNTP; N0447S) were purchased from New England Biolabs. .. Uracil DNA glycosylase (catalog no. 78310) and PCR nucleotide mix with dUTP (catalog no. 77330) were acquired from USB (Cleveland, OH).

    Cytometry:

    Article Title: One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
    Article Snippet: PCRs were performed with Taq polymerase (M0273; NEB). .. Analysis of flow cytometry data for presentation was performed using FlowJo software v10.

    Real-time Polymerase Chain Reaction:

    Article Title: Characterization of EvaGreen and the implication of its physicochemical properties for qPCR applications
    Article Snippet: Paragraph title: Real-time PCR and post-PCR analysis ... To test if different enzymes may affect the relative performance of EG and SG differently, the above experiments (except for the comparison experiment with Power SYBR kit) were repeated with Taq from NEB (Catalog # M0273S), Taq from Promega (Catalog # M1661), Taq from Fermentas(Catalog # EP0401) and home-made Taq.

    Incubation:

    Article Title: Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells
    Article Snippet: General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5® High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. Vector and insert were mixed in 1:3 molar ratios (unless otherwise specified) and ligated in the presence of T4 DNA ligase (NEB, #M0202) at 4o C for 18 h. 100 μl of E.coli DH5α as competent cells was transformed with 5 μl of vector-insert ligation mix and selected on LB agar plates containing 100 μg/mL ampicillin at 30°C overnight incubation.

    Article Title: ATG-18 and EPG-6 are Both Required for Autophagy but Differentially Contribute to Lifespan Control in Caenorhabditis elegans
    Article Snippet: Genotyping by Single-Worm Lysis PCR For single worm lysis (SWL), PCR worms were lysed singly in 10 μL single-worm lysis-buffer (SWL-buffer) (0.05 M KCl, 2.5 mM MgCl2 ·6H2 O, 0.01 MTris, 0.45% Tween-20, 0.45% Triton-X) supplemented with 1 μL proteinase K (NEB, P8102S), and incubated at 65 °C for 1 h, then at 95 °C for 20 min. .. For one PCR reaction, 25 units of Taq DNA polymerase (NEB, M0273) with standard Taq buffer (NEB, M0273L), 200 μM dNTP (NEB, N0446S), 0.5 μM of both primers (Sigma–Aldrich, Munich, Germany), and 5 μL DNA was used.

    Article Title: Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth
    Article Snippet: PCR reactions were run in a final volume of 25 μl containing 200 ng of bacterial DNA extracted with a standard protocol (Zhong et al., ), 200 nM of each primer (1DF: AGA CTT TTT GGC AGG CAA AGG, 1EF: ACA AAC CAC CAG TGC CTA AGG, 1B2R: GCT CTT CGA GAT CAC TGG GC), 200 nM of dNTP, and 1U of Taq DNA polymerase (NEB M0273S). .. Bacteria were subcultured in MRS or modified TYI-S-33 medium (MTYI) (Pérez et al., ) and incubated anaerobically for 12–18 h at 37°C.

    Expressing:

    Article Title: Characterization and Mechanism of Stress-induced Translocation of 78-Kilodalton Glucose-regulated Protein (GRP78) to the Cell Surface *
    Article Snippet: .. The construction of expression plasmid for GRP78 substrate binding domain (SBD) with KDEL motif at the C terminus was generated by PCR amplification from FLAG-GRP78 (WT) expression plasmid using TaqDNA polymerase (M0273S, New England Biolabs, Ipswich, MA) and primers 5′-TATTATCCCGGGGTCCAGGCTGGTGTGCTCTCTG-3′ and 5′-TTATATGTCGACCTATTACAACTCATCTTTGGTGACTTCAATCTGTGGGAC-3′. .. The PCR product was inserted in-frame into pDisplay expression vector (Life Technologies) between XmaI and SalI sites.

    Article Title: Caloric restriction engages hepatic RNA processing mechanisms in rhesus monkeys
    Article Snippet: Five exon changes identified by DEXseq were selected for validation via polymerase chain reaction (PCR); in selecting these genes, we were guided by gene architecture, expression level and degree of fold change calculated by DEXseq, and feasibility of primer design. .. The cDNA was diluted 1:4 with water, and 3 μL of diluted cDNA was used as the template for PCR (New England Biolabs #M0273S), which was run according to the manufacturer’s specifications.

    Modification:

    Article Title: Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth
    Article Snippet: PCR reactions were run in a final volume of 25 μl containing 200 ng of bacterial DNA extracted with a standard protocol (Zhong et al., ), 200 nM of each primer (1DF: AGA CTT TTT GGC AGG CAA AGG, 1EF: ACA AAC CAC CAG TGC CTA AGG, 1B2R: GCT CTT CGA GAT CAC TGG GC), 200 nM of dNTP, and 1U of Taq DNA polymerase (NEB M0273S). .. Bacteria were subcultured in MRS or modified TYI-S-33 medium (MTYI) (Pérez et al., ) and incubated anaerobically for 12–18 h at 37°C.

    Transformation Assay:

    Article Title: Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells
    Article Snippet: General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5® High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. Vector and insert were mixed in 1:3 molar ratios (unless otherwise specified) and ligated in the presence of T4 DNA ligase (NEB, #M0202) at 4o C for 18 h. 100 μl of E.coli DH5α as competent cells was transformed with 5 μl of vector-insert ligation mix and selected on LB agar plates containing 100 μg/mL ampicillin at 30°C overnight incubation.

    Gel Purification:

    Article Title: Cystathionine ?-lyase: clinical, metabolic, genetic, and structural studies
    Article Snippet: PCR was performed using either PfuTurbo DNA polymerase (Stratagene 600250, Cedar Creek, TX) or Taq DNA polymerase (New England Biolabs M0273S, Ipswich, MA). .. After PCR, the product was purified using the Qiagen QIAquick PCR Purification kit (Qiagen Sciences 28104, MD) and/or by gel purification using the Qiagen QIAquick Gel Extraction kit (Qiagen Sciences 28704, MD).

    High Performance Liquid Chromatography:

    Article Title: Characterization of Protein–DNA Interactions Using Protein Microarrays
    Article Snippet: .. Agarose gel (3%) < R > Annealing buffer for PDI (10×) < R > Base buffer (2×) Bovine serum albumin fraction V, heat shock (BSA; Roche 03116999001) DL-Dithiothreitol (DTT; Sigma) (1 M) DNA ladders (20 bp) and 6× DNA loading dye (Fermentas SM1323) dNTPs, high-purity solution (GE Healthcare 27-2035-02) Illustra MicroSpin G-25 Columns (GE Healthcare 27-5325-01) Oligonucleotides, high performance liquid chromatography (HPLC)-purified (Integrated DNA Technologies) Phenylmethylsulfonyl fluoride (PMSF) (0.2 M) Protease Inhibitor Cocktail Tablets, complete (Roche 11873580001) SYBR Gold Stain (Invitrogen S11494) Taq DNA polymerase and 10× Standard Taq Reaction Buffer (NEB M0273) Wash buffer < R > Prepare the wash buffer by diluting base buffer (2×) with an equal volume of H2 O. .. Aluminum foil Centrifuge, benchtop, with microplate rotor (Thermo 75004375) Coverslips, LifterSlip (Thermo Scientific 25X60I24789001LS) Equipment for agarose gel electrophoresis Filter, 0.45-μm (VWR) Humidified chamber Prepare the chamber by filling an empty pipette tip box with about half an inch of sterile water.

    Flow Cytometry:

    Article Title: One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
    Article Snippet: PCRs were performed with Taq polymerase (M0273; NEB). .. Analysis of flow cytometry data for presentation was performed using FlowJo software v10.

    Ligation:

    Article Title: Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells
    Article Snippet: General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5® High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. Vector and insert were mixed in 1:3 molar ratios (unless otherwise specified) and ligated in the presence of T4 DNA ligase (NEB, #M0202) at 4o C for 18 h. 100 μl of E.coli DH5α as competent cells was transformed with 5 μl of vector-insert ligation mix and selected on LB agar plates containing 100 μg/mL ampicillin at 30°C overnight incubation.

    Protease Inhibitor:

    Article Title: Characterization of Protein–DNA Interactions Using Protein Microarrays
    Article Snippet: .. Agarose gel (3%) < R > Annealing buffer for PDI (10×) < R > Base buffer (2×) Bovine serum albumin fraction V, heat shock (BSA; Roche 03116999001) DL-Dithiothreitol (DTT; Sigma) (1 M) DNA ladders (20 bp) and 6× DNA loading dye (Fermentas SM1323) dNTPs, high-purity solution (GE Healthcare 27-2035-02) Illustra MicroSpin G-25 Columns (GE Healthcare 27-5325-01) Oligonucleotides, high performance liquid chromatography (HPLC)-purified (Integrated DNA Technologies) Phenylmethylsulfonyl fluoride (PMSF) (0.2 M) Protease Inhibitor Cocktail Tablets, complete (Roche 11873580001) SYBR Gold Stain (Invitrogen S11494) Taq DNA polymerase and 10× Standard Taq Reaction Buffer (NEB M0273) Wash buffer < R > Prepare the wash buffer by diluting base buffer (2×) with an equal volume of H2 O. .. Aluminum foil Centrifuge, benchtop, with microplate rotor (Thermo 75004375) Coverslips, LifterSlip (Thermo Scientific 25X60I24789001LS) Equipment for agarose gel electrophoresis Filter, 0.45-μm (VWR) Humidified chamber Prepare the chamber by filling an empty pipette tip box with about half an inch of sterile water.

    Generated:

    Article Title: Characterization and Mechanism of Stress-induced Translocation of 78-Kilodalton Glucose-regulated Protein (GRP78) to the Cell Surface *
    Article Snippet: .. The construction of expression plasmid for GRP78 substrate binding domain (SBD) with KDEL motif at the C terminus was generated by PCR amplification from FLAG-GRP78 (WT) expression plasmid using TaqDNA polymerase (M0273S, New England Biolabs, Ipswich, MA) and primers 5′-TATTATCCCGGGGTCCAGGCTGGTGTGCTCTCTG-3′ and 5′-TTATATGTCGACCTATTACAACTCATCTTTGGTGACTTCAATCTGTGGGAC-3′. .. The PCR product was inserted in-frame into pDisplay expression vector (Life Technologies) between XmaI and SalI sites.

    Article Title: Caloric restriction engages hepatic RNA processing mechanisms in rhesus monkeys
    Article Snippet: Differential exon usage was performed using DEXseq ( ) (v. 1.16.10) on exon feature counts generated by HTSeq ( ) (v. 0.6.1p1). .. The cDNA was diluted 1:4 with water, and 3 μL of diluted cDNA was used as the template for PCR (New England Biolabs #M0273S), which was run according to the manufacturer’s specifications.

    DNA Sequencing:

    Article Title: Cystathionine ?-lyase: clinical, metabolic, genetic, and structural studies
    Article Snippet: PCR was performed using either PfuTurbo DNA polymerase (Stratagene 600250, Cedar Creek, TX) or Taq DNA polymerase (New England Biolabs M0273S, Ipswich, MA). .. 50 ng of the purified PCR product along with 10 pmols of the primer used for PCR in a total of 17 μl was then sent for sequencing to the Colorado University Cancer Center DNA Sequencing & Analysis Core (CUCCC).

    Sequencing:

    Article Title: Characterization and Mechanism of Stress-induced Translocation of 78-Kilodalton Glucose-regulated Protein (GRP78) to the Cell Surface *
    Article Snippet: The construction of expression plasmid for GRP78 substrate binding domain (SBD) with KDEL motif at the C terminus was generated by PCR amplification from FLAG-GRP78 (WT) expression plasmid using TaqDNA polymerase (M0273S, New England Biolabs, Ipswich, MA) and primers 5′-TATTATCCCGGGGTCCAGGCTGGTGTGCTCTCTG-3′ and 5′-TTATATGTCGACCTATTACAACTCATCTTTGGTGACTTCAATCTGTGGGAC-3′. .. The caveolin-1-SNAP expression plasmid was produced by PCR amplification of the SNAP coding sequence from a pSNAP-tag(m) plasmid (New England Biolabs) using forward primer 5′-AACAAACCGCGGATGGACAAA-3′ and reverse primer 5′-AACAAATCTAGATCAGG TACC-3′.

    Article Title: Cystathionine ?-lyase: clinical, metabolic, genetic, and structural studies
    Article Snippet: PCR was performed using either PfuTurbo DNA polymerase (Stratagene 600250, Cedar Creek, TX) or Taq DNA polymerase (New England Biolabs M0273S, Ipswich, MA). .. 50 ng of the purified PCR product along with 10 pmols of the primer used for PCR in a total of 17 μl was then sent for sequencing to the Colorado University Cancer Center DNA Sequencing & Analysis Core (CUCCC).

    Article Title: One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
    Article Snippet: Paragraph title: Single-cell Igh and Igk PCR and sequence analysis ... PCRs were performed with Taq polymerase (M0273; NEB).

    Binding Assay:

    Article Title: Characterization and Mechanism of Stress-induced Translocation of 78-Kilodalton Glucose-regulated Protein (GRP78) to the Cell Surface *
    Article Snippet: .. The construction of expression plasmid for GRP78 substrate binding domain (SBD) with KDEL motif at the C terminus was generated by PCR amplification from FLAG-GRP78 (WT) expression plasmid using TaqDNA polymerase (M0273S, New England Biolabs, Ipswich, MA) and primers 5′-TATTATCCCGGGGTCCAGGCTGGTGTGCTCTCTG-3′ and 5′-TTATATGTCGACCTATTACAACTCATCTTTGGTGACTTCAATCTGTGGGAC-3′. .. The PCR product was inserted in-frame into pDisplay expression vector (Life Technologies) between XmaI and SalI sites.

    Cellular Antioxidant Activity Assay:

    Article Title: Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth
    Article Snippet: .. PCR reactions were run in a final volume of 25 μl containing 200 ng of bacterial DNA extracted with a standard protocol (Zhong et al., ), 200 nM of each primer (1DF: AGA CTT TTT GGC AGG CAA AGG, 1EF: ACA AAC CAC CAG TGC CTA AGG, 1B2R: GCT CTT CGA GAT CAC TGG GC), 200 nM of dNTP, and 1U of Taq DNA polymerase (NEB M0273S). .. Reactions were initiated with an initial denaturation for 5 min at 95°C followed by 35 cycles at 95°C for 30 s, 50°C for 30 s and 72°C for 1 min. PCR products were visualized on 0.8% agarose gel containing 0.5 ng/μl of BET and images were captured with a camera coupled with Biocapt software, Vilber Lourmat.

    Mutagenesis:

    Article Title: Characterization and Mechanism of Stress-induced Translocation of 78-Kilodalton Glucose-regulated Protein (GRP78) to the Cell Surface *
    Article Snippet: The mutants of GRP78 were generated using FLAG-GRP78 as template and following the protocol of QuikChange site-directed mutagenesis (Stratagene, La Jolla, CA). .. The construction of expression plasmid for GRP78 substrate binding domain (SBD) with KDEL motif at the C terminus was generated by PCR amplification from FLAG-GRP78 (WT) expression plasmid using TaqDNA polymerase (M0273S, New England Biolabs, Ipswich, MA) and primers 5′-TATTATCCCGGGGTCCAGGCTGGTGTGCTCTCTG-3′ and 5′-TTATATGTCGACCTATTACAACTCATCTTTGGTGACTTCAATCTGTGGGAC-3′.

    Article Title: ATG-18 and EPG-6 are Both Required for Autophagy but Differentially Contribute to Lifespan Control in Caenorhabditis elegans
    Article Snippet: For one PCR reaction, 25 units of Taq DNA polymerase (NEB, M0273) with standard Taq buffer (NEB, M0273L), 200 μM dNTP (NEB, N0446S), 0.5 μM of both primers (Sigma–Aldrich, Munich, Germany), and 5 μL DNA was used. .. For genotyping of epg-6(bp242) tetra-primer amplification-refractory mutation system PCR (tetra-primer ARMS-PCR) was applied [ ].

    Isolation:

    Article Title: Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth
    Article Snippet: Culture of Lactobacillus johnsonii La1 and production of bacterial supernatant L. johnsonii La1 (CNRZ 1897, NCC533) was kindly provided by Pascal Quénée (INRA Jouy en Josas, Equipe Atalis, France) and was isolated from LC1 product in 1996 (Chambourcy, France). .. PCR reactions were run in a final volume of 25 μl containing 200 ng of bacterial DNA extracted with a standard protocol (Zhong et al., ), 200 nM of each primer (1DF: AGA CTT TTT GGC AGG CAA AGG, 1EF: ACA AAC CAC CAG TGC CTA AGG, 1B2R: GCT CTT CGA GAT CAC TGG GC), 200 nM of dNTP, and 1U of Taq DNA polymerase (NEB M0273S).

    Purification:

    Article Title: Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells
    Article Snippet: General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5® High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. PCR amplified or restricted DNA fragments were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co, #740609).

    Article Title: Use of FabV-Triclosan Plasmid Selection System for Efficient Expression and Production of Recombinant Proteins in Escherichia coli
    Article Snippet: General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5 High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. PCR amplified or restricted DNA fragments were purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel GmbH & Co, #740609).

    Article Title: Combined magnetic tweezers and micro-mirror total internal reflection fluorescence microscope for single molecule manipulation and visualization
    Article Snippet: .. Taq DNA polymerase (M0273, NEB) and supplied buffer 100 μM Deoxynucleotide (dNTP) Solution Biotin-16-dUTP (11093070910, Roche) Digoxigenin-11-dUTP (11093088910, Roche) DNA purification kit (QIAquick PCR purification kit, Qiagen) .. BsaI-HF (R3535, NEB) and supplied buffer Products from PCR reactions DNA clean-up kit (11732668001, Roche)

    Article Title: Cystathionine ?-lyase: clinical, metabolic, genetic, and structural studies
    Article Snippet: Following RNA purification, cDNA was made using the SuperScript First Strand kit (Invitrogen 12371-019; Carlsbad, CA). .. PCR was performed using either PfuTurbo DNA polymerase (Stratagene 600250, Cedar Creek, TX) or Taq DNA polymerase (New England Biolabs M0273S, Ipswich, MA).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Cystathionine ?-lyase: clinical, metabolic, genetic, and structural studies
    Article Snippet: Paragraph title: Identification of mutations using RT-PCR ... PCR was performed using either PfuTurbo DNA polymerase (Stratagene 600250, Cedar Creek, TX) or Taq DNA polymerase (New England Biolabs M0273S, Ipswich, MA).

    Gel Extraction:

    Article Title: Cystathionine ?-lyase: clinical, metabolic, genetic, and structural studies
    Article Snippet: PCR was performed using either PfuTurbo DNA polymerase (Stratagene 600250, Cedar Creek, TX) or Taq DNA polymerase (New England Biolabs M0273S, Ipswich, MA). .. After PCR, the product was purified using the Qiagen QIAquick PCR Purification kit (Qiagen Sciences 28104, MD) and/or by gel purification using the Qiagen QIAquick Gel Extraction kit (Qiagen Sciences 28704, MD).

    IA:

    Article Title: Mechanical Disruption of Lysis-Resistant Bacterial Cells by Use of a Miniature, Low-Power, Disposable Device ▿Mechanical Disruption of Lysis-Resistant Bacterial Cells by Use of a Miniature, Low-Power, Disposable Device ▿ †
    Article Snippet: Standard Taq buffer (B9014S), Taq DNA polymerase (M0273L), and deoxynucleotide triphosphate (dNTP; N0447S) were purchased from New England Biolabs. .. Oligonucleotide primers were ordered from Integrated DNA Technologies (Coralville, IA) and Sigma-Aldrich (St. Louis, MO).

    Plasmid Preparation:

    Article Title: Characterization and Mechanism of Stress-induced Translocation of 78-Kilodalton Glucose-regulated Protein (GRP78) to the Cell Surface *
    Article Snippet: .. The construction of expression plasmid for GRP78 substrate binding domain (SBD) with KDEL motif at the C terminus was generated by PCR amplification from FLAG-GRP78 (WT) expression plasmid using TaqDNA polymerase (M0273S, New England Biolabs, Ipswich, MA) and primers 5′-TATTATCCCGGGGTCCAGGCTGGTGTGCTCTCTG-3′ and 5′-TTATATGTCGACCTATTACAACTCATCTTTGGTGACTTCAATCTGTGGGAC-3′. .. The PCR product was inserted in-frame into pDisplay expression vector (Life Technologies) between XmaI and SalI sites.

    Article Title: Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells
    Article Snippet: General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5® High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. Plasmid DNA was purified using Wizard® Plus SV Minipreps DNA Purification System (Promega, #A1465).

    Article Title: Use of FabV-Triclosan Plasmid Selection System for Efficient Expression and Production of Recombinant Proteins in Escherichia coli
    Article Snippet: General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5 High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. Plasmid DNA was purified using Wizard® Plus SV Minipreps DNA Purification System (Promega, #A1465).

    Article Title: Examining Sources of Error in PCR by Single-Molecule Sequencing
    Article Snippet: Q5 High Fidelity DNA Polymerase (#M0491), Phusion High Fidelity DNA Polymerase (#M0530), Deep Vent DNA Polymerase (#M0258), and Taq DNA polymerase (#M0273) are supplied by New England Biolabs. .. Reaction conditions ranged from: 1X reaction buffer (provided with the enzyme), 200–300 μM each dNTP, 0.2–1 μM forward and reverse primers, 100 pg/μl plasmid template (linearized with a restriction enzyme and treated with PreCR) and polymerase.

    Software:

    Article Title: Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth
    Article Snippet: PCR reactions were run in a final volume of 25 μl containing 200 ng of bacterial DNA extracted with a standard protocol (Zhong et al., ), 200 nM of each primer (1DF: AGA CTT TTT GGC AGG CAA AGG, 1EF: ACA AAC CAC CAG TGC CTA AGG, 1B2R: GCT CTT CGA GAT CAC TGG GC), 200 nM of dNTP, and 1U of Taq DNA polymerase (NEB M0273S). .. Reactions were initiated with an initial denaturation for 5 min at 95°C followed by 35 cycles at 95°C for 30 s, 50°C for 30 s and 72°C for 1 min. PCR products were visualized on 0.8% agarose gel containing 0.5 ng/μl of BET and images were captured with a camera coupled with Biocapt software, Vilber Lourmat.

    Article Title: One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation
    Article Snippet: PCRs were performed with Taq polymerase (M0273; NEB). .. Analysis of flow cytometry data for presentation was performed using FlowJo software v10.

    Agarose Gel Electrophoresis:

    Article Title: Caloric restriction engages hepatic RNA processing mechanisms in rhesus monkeys
    Article Snippet: The cDNA was diluted 1:4 with water, and 3 μL of diluted cDNA was used as the template for PCR (New England Biolabs #M0273S), which was run according to the manufacturer’s specifications. .. Following PCR amplification, products were analyzed via a 1.3% agarose gel (containing 83.3 ng/mL ethidium bromide), which was visualized using a UV transilluminator.

    Article Title: Deconjugated Bile Salts Produced by Extracellular Bile-Salt Hydrolase-Like Activities from the Probiotic Lactobacillus johnsonii La1 Inhibit Giardia duodenalis In vitro Growth
    Article Snippet: PCR reactions were run in a final volume of 25 μl containing 200 ng of bacterial DNA extracted with a standard protocol (Zhong et al., ), 200 nM of each primer (1DF: AGA CTT TTT GGC AGG CAA AGG, 1EF: ACA AAC CAC CAG TGC CTA AGG, 1B2R: GCT CTT CGA GAT CAC TGG GC), 200 nM of dNTP, and 1U of Taq DNA polymerase (NEB M0273S). .. Reactions were initiated with an initial denaturation for 5 min at 95°C followed by 35 cycles at 95°C for 30 s, 50°C for 30 s and 72°C for 1 min. PCR products were visualized on 0.8% agarose gel containing 0.5 ng/μl of BET and images were captured with a camera coupled with Biocapt software, Vilber Lourmat.

    Article Title: Characterization of Protein–DNA Interactions Using Protein Microarrays
    Article Snippet: .. Agarose gel (3%) < R > Annealing buffer for PDI (10×) < R > Base buffer (2×) Bovine serum albumin fraction V, heat shock (BSA; Roche 03116999001) DL-Dithiothreitol (DTT; Sigma) (1 M) DNA ladders (20 bp) and 6× DNA loading dye (Fermentas SM1323) dNTPs, high-purity solution (GE Healthcare 27-2035-02) Illustra MicroSpin G-25 Columns (GE Healthcare 27-5325-01) Oligonucleotides, high performance liquid chromatography (HPLC)-purified (Integrated DNA Technologies) Phenylmethylsulfonyl fluoride (PMSF) (0.2 M) Protease Inhibitor Cocktail Tablets, complete (Roche 11873580001) SYBR Gold Stain (Invitrogen S11494) Taq DNA polymerase and 10× Standard Taq Reaction Buffer (NEB M0273) Wash buffer < R > Prepare the wash buffer by diluting base buffer (2×) with an equal volume of H2 O. .. Aluminum foil Centrifuge, benchtop, with microplate rotor (Thermo 75004375) Coverslips, LifterSlip (Thermo Scientific 25X60I24789001LS) Equipment for agarose gel electrophoresis Filter, 0.45-μm (VWR) Humidified chamber Prepare the chamber by filling an empty pipette tip box with about half an inch of sterile water.

    Produced:

    Article Title: Characterization and Mechanism of Stress-induced Translocation of 78-Kilodalton Glucose-regulated Protein (GRP78) to the Cell Surface *
    Article Snippet: The construction of expression plasmid for GRP78 substrate binding domain (SBD) with KDEL motif at the C terminus was generated by PCR amplification from FLAG-GRP78 (WT) expression plasmid using TaqDNA polymerase (M0273S, New England Biolabs, Ipswich, MA) and primers 5′-TATTATCCCGGGGTCCAGGCTGGTGTGCTCTCTG-3′ and 5′-TTATATGTCGACCTATTACAACTCATCTTTGGTGACTTCAATCTGTGGGAC-3′. .. The caveolin-1-SNAP expression plasmid was produced by PCR amplification of the SNAP coding sequence from a pSNAP-tag(m) plasmid (New England Biolabs) using forward primer 5′-AACAAACCGCGGATGGACAAA-3′ and reverse primer 5′-AACAAATCTAGATCAGG TACC-3′.

    Concentration Assay:

    Article Title: ATG-18 and EPG-6 are Both Required for Autophagy but Differentially Contribute to Lifespan Control in Caenorhabditis elegans
    Article Snippet: For one PCR reaction, 25 units of Taq DNA polymerase (NEB, M0273) with standard Taq buffer (NEB, M0273L), 200 μM dNTP (NEB, N0446S), 0.5 μM of both primers (Sigma–Aldrich, Munich, Germany), and 5 μL DNA was used. .. For ARMS-PCR, the inner primers were used at 1 μM, the outer primers at 0.1 μM final concentration.

    DNA Purification:

    Article Title: Expression of Functional Anti-p24 scFv 183-H12-5C in HEK293T and Jurkat T Cells
    Article Snippet: General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5® High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. Plasmid DNA was purified using Wizard® Plus SV Minipreps DNA Purification System (Promega, #A1465).

    Article Title: Use of FabV-Triclosan Plasmid Selection System for Efficient Expression and Production of Recombinant Proteins in Escherichia coli
    Article Snippet: General molecular biology techniques PCR amplifications of DNA fragments intended for cloning purposes was carried out using Q5 High-Fidelity DNA Polymerase (NEB, #M0491), whereas Taq DNA Polymerase (NEB, #M0273) was used for routine colony PCR. .. Plasmid DNA was purified using Wizard® Plus SV Minipreps DNA Purification System (Promega, #A1465).

    Article Title: Combined magnetic tweezers and micro-mirror total internal reflection fluorescence microscope for single molecule manipulation and visualization
    Article Snippet: .. Taq DNA polymerase (M0273, NEB) and supplied buffer 100 μM Deoxynucleotide (dNTP) Solution Biotin-16-dUTP (11093070910, Roche) Digoxigenin-11-dUTP (11093088910, Roche) DNA purification kit (QIAquick PCR purification kit, Qiagen) .. BsaI-HF (R3535, NEB) and supplied buffer Products from PCR reactions DNA clean-up kit (11732668001, Roche)

    Lysis:

    Article Title: ATG-18 and EPG-6 are Both Required for Autophagy but Differentially Contribute to Lifespan Control in Caenorhabditis elegans
    Article Snippet: Paragraph title: 2.5. Genotyping by Single-Worm Lysis PCR ... For one PCR reaction, 25 units of Taq DNA polymerase (NEB, M0273) with standard Taq buffer (NEB, M0273L), 200 μM dNTP (NEB, N0446S), 0.5 μM of both primers (Sigma–Aldrich, Munich, Germany), and 5 μL DNA was used.

    Staining:

    Article Title: Characterization of Protein–DNA Interactions Using Protein Microarrays
    Article Snippet: .. Agarose gel (3%) < R > Annealing buffer for PDI (10×) < R > Base buffer (2×) Bovine serum albumin fraction V, heat shock (BSA; Roche 03116999001) DL-Dithiothreitol (DTT; Sigma) (1 M) DNA ladders (20 bp) and 6× DNA loading dye (Fermentas SM1323) dNTPs, high-purity solution (GE Healthcare 27-2035-02) Illustra MicroSpin G-25 Columns (GE Healthcare 27-5325-01) Oligonucleotides, high performance liquid chromatography (HPLC)-purified (Integrated DNA Technologies) Phenylmethylsulfonyl fluoride (PMSF) (0.2 M) Protease Inhibitor Cocktail Tablets, complete (Roche 11873580001) SYBR Gold Stain (Invitrogen S11494) Taq DNA polymerase and 10× Standard Taq Reaction Buffer (NEB M0273) Wash buffer < R > Prepare the wash buffer by diluting base buffer (2×) with an equal volume of H2 O. .. Aluminum foil Centrifuge, benchtop, with microplate rotor (Thermo 75004375) Coverslips, LifterSlip (Thermo Scientific 25X60I24789001LS) Equipment for agarose gel electrophoresis Filter, 0.45-μm (VWR) Humidified chamber Prepare the chamber by filling an empty pipette tip box with about half an inch of sterile water.

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    New England Biolabs taq dna polymerase with standard taq buffer
    Taq Dna Polymerase With Standard Taq Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna polymerase with standard taq buffer/product/New England Biolabs
    Average 90 stars, based on 8 article reviews
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    taq dna polymerase with standard taq buffer - by Bioz Stars, 2020-01
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