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Name:
phi29 DNA Polymerase
Description:
phi29 DNA Polymerase 1 250 units
Catalog Number:
m0269l
Price:
228
Size:
1 250 units
Category:
DNA Polymerases
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New England Biolabs
bsa
phi29 DNA Polymerase 1 250 units
https://www.bioz.com/result/bsa/product/New England Biolabs
Average 95 stars, based on 7 article reviews
Price from $9.99 to $1999.99
phi29 DNA Polymerase 1 250 units
https://www.bioz.com/result/bsa/product/New England Biolabs
Average 95 stars, based on 7 article reviews
Price from $9.99 to $1999.99
bsa - by Bioz Stars,
2020-02
95/100 stars
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Amplification:Article Title: Development of an easy and cost-effective method for non-invasive genotyping of insects Article Snippet: The COI region from M . separata frass DNA was amplified from all samples using Multiple Displacement Amplification (MDA), a non-PCR-based DNA amplification technique that can rapidly amplify minute amounts of DNA samples to a reasonable quantity by using the high fidelity, processivity and standard displacement capacity of phi 29 DNA polymerase [ ]. .. The MDA reaction was carried out in a 20 μl final volume containing 2 μl 10× reaction buffer with 1.6 μl dNTP mix (2.5 μM each), 0.5 μl Article Title: Large Circular Plasmids from Groundwater Plasmidomes Span Multiple Incompatibility Groups and Are Enriched in Multimetal Resistance Genes Article Snippet: .. The DNA was then amplified with Article Title: TZAP: a telomere-associated protein involved in telomere length control Article Snippet: The method for T-circle amplification was performed as described in ( ) with some modifications. .. The annealed sample was divided in two and half (10 μl) was incubated at 30°C for 12 h in the presence of dNTPs (200 μM each), 0.2 mg/ml BSA (NEB), 1X phi29 Buffer (NEB) and 7.5 U Article Title: Rolling Circle Amplification, a Powerful Tool for Genetic and Functional Studies of Complete Hepatitis B Virus Genomes from Low-Level Infections and for Directly Probing Covalently Closed Circular DNA ▿ Article Snippet: Paragraph title: Rolling circle amplification. ... Sample mixtures were combined with 10 μl of reaction mixture containing: 1× Phi29 buffer, the eight primers at a concentration of 0.5 μM each, 0.4 mg/ml of bovine serum albumin, 4 mM of dithiothreitol, 2 mM of each dNTP, and 10 U of the Article Title: DNA amplification method tolerant to sample degradation Article Snippet: .. The denatured DNA was amplified using 0.3 μL Article Title: Characterization of a novel gyrovirus in human stool and chicken meat Article Snippet: .. To sequence the complete genome of the putative novel gyrovirus, nucleic acid extracted from the original sample was subjected to rolling circle DNA amplification using Article Title: A novel method for detection of HBVcccDNA in hepatocytes using rolling circle amplification combined with in situ PCR Article Snippet: .. In order to increase the efficiency of amplification, RCA was divided into two steps: First-step, 14 μl primer combining reaction mixture containing primers at a operative solutionconcentration of 0.5 μmol/l each , 2 μl reaction buffer and 11 μl ddH2 O were loaded onto the tissue sections on glass slides and sealed with rubber cement, followed by heating at 95°C for 6 min, and then placed on ice for 15 min. Second-step the RCA amplification was as follows: 23 μl of reaction mixture was added to tissue slides containing primers at a operative solution concentration of 0.5 μmol/l each, 10 mg/ml bovine serum albumin (BSA), 0.32 mmol/l of dNTP, 20 U of the Article Title: Imaging single DNA molecules for high precision NIPT Article Snippet: .. Rolling circle amplification (RCA) was performed by adding 11.2 µl RCA master mix consisting of 1.07 µM of a RCA primer oligonucleotide (IDT) designed to be complementary to a sequence present in the backbone region of each DNA circle, 5.4 mM dNTP solution mix (New England Biolabs) and 20 U Whole Genome Amplification:Article Title: DNA amplification method tolerant to sample degradation Article Snippet: Paragraph title: RCA–RCA whole genome amplification ... The denatured DNA was amplified using 0.3 μL Article Title: Whole genome sequencing of amplified Plasmodium knowlesi DNA from unprocessed blood reveals genetic exchange events between Malaysian Peninsular and Borneo subpopulations Article Snippet: Paragraph title: Selective whole genome amplification ... SWGA reactions were performed containing a maximum of 50 ng of total input genomic DNA (and a minimum of 5 ng), 5 µl of 10 x Synthesized:Article Title: Microfluidic Exponential Rolling Circle Amplification for Sensitive microRNA Detection Directly from Biological Samples Article Snippet: Synthetic oligonucleotides and miRNAs were synthesized and purified with polyacrylamide gel electrophoresis (PAGE) by Integrated DNA Technologies (IDT, Coralville, IA). .. SYBR Green Assay:Article Title: Large Circular Plasmids from Groundwater Plasmidomes Span Multiple Incompatibility Groups and Are Enriched in Multimetal Resistance Genes Article Snippet: The DNA was then amplified with Article Title: Repressor logic modules assembled by rolling circle amplification platform to construct a set of logic gates Article Snippet: .. Circularization and RCA reaction For circularization, The 5′- and 3′-portions of the circular template (1 μM) were hybridized with a splint oligonucleotide (1 μM) in a head-to-tail way, and then were ligated by T4 DNA ligase (175 U) in ligation buffer containing 50 mM Tris pH 8.0, 10 mM MgCl2 , 5 mM DTT and 0.1 mM ATP at 16 °C for 60 min. RCA reaction solution was composed of 0.05 pmol ligated circular template, 50 mM Tris, 10 mM MgCl2 , 10 mM (NH4 )2 SO4 , 4 mM DTT (pH 7.5), SYBR Green II (1:10000) and Article Title: Microfluidic Exponential Rolling Circle Amplification for Sensitive microRNA Detection Directly from Biological Samples Article Snippet: Incubation:Article Title: Development of an easy and cost-effective method for non-invasive genotyping of insects Article Snippet: The MDA reaction was carried out in a 20 μl final volume containing 2 μl 10× reaction buffer with 1.6 μl dNTP mix (2.5 μM each), 0.5 μl Article Title: On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires Article Snippet: .. Subsequently, 127.5 μl of multiple-displacement amplification (MDA) master mix (6 μl of 25 mM dNTP mix [Roche], 15 μl of 10x Article Title: TZAP: a telomere-associated protein involved in telomere length control Article Snippet: .. The annealed sample was divided in two and half (10 μl) was incubated at 30°C for 12 h in the presence of dNTPs (200 μM each), 0.2 mg/ml BSA (NEB), 1X phi29 Buffer (NEB) and 7.5 U Article Title: Imaging single DNA molecules for high precision NIPT Article Snippet: Rolling circle amplification (RCA) was performed by adding 11.2 µl RCA master mix consisting of 1.07 µM of a RCA primer oligonucleotide (IDT) designed to be complementary to a sequence present in the backbone region of each DNA circle, 5.4 mM dNTP solution mix (New England Biolabs) and 20 U Activity Assay:Article Title: Rolling Circle Amplification, a Powerful Tool for Genetic and Functional Studies of Complete Hepatitis B Virus Genomes from Low-Level Infections and for Directly Probing Covalently Closed Circular DNA ▿ Article Snippet: To protect primers from the strong proofreading activity of the Phi29 polymerase, the two 3′ bases were phosphorothioate modified. .. Sample mixtures were combined with 10 μl of reaction mixture containing: 1× Phi29 buffer, the eight primers at a concentration of 0.5 μM each, 0.4 mg/ml of bovine serum albumin, 4 mM of dithiothreitol, 2 mM of each dNTP, and 10 U of the Modification:Article Title: Rolling Circle Amplification, a Powerful Tool for Genetic and Functional Studies of Complete Hepatitis B Virus Genomes from Low-Level Infections and for Directly Probing Covalently Closed Circular DNA ▿ Article Snippet: To protect primers from the strong proofreading activity of the Phi29 polymerase, the two 3′ bases were phosphorothioate modified. .. Sample mixtures were combined with 10 μl of reaction mixture containing: 1× Phi29 buffer, the eight primers at a concentration of 0.5 μM each, 0.4 mg/ml of bovine serum albumin, 4 mM of dithiothreitol, 2 mM of each dNTP, and 10 U of the Filtration:Article Title: On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires Article Snippet: Subsequently, 127.5 μl of multiple-displacement amplification (MDA) master mix (6 μl of 25 mM dNTP mix [Roche], 15 μl of 10x Phi29 DNA polymerase reaction buffer [NEB], 7.5 μl of 1 μg/μl exonuclease-resistant random hexamers, 7.5 μl of 0.1 M dithiothreitol, 93 μl of RNase-free water) was added to each reaction, and the mixture was split into 3 tubes at 47.5 μl each and incubated at 95°C for 5 min and then at 4°C during the addition of 2.5 μl of Hybridization:Article Title: TZAP: a telomere-associated protein involved in telomere length control Article Snippet: The annealed sample was divided in two and half (10 μl) was incubated at 30°C for 12 h in the presence of dNTPs (200 μM each), 0.2 mg/ml BSA (NEB), 1X phi29 Buffer (NEB) and 7.5 U Article Title: Imaging single DNA molecules for high precision NIPT Article Snippet: Following hybridization and ligation, 39 µl of an exonuclease master mix consisting of 20 U Exonuclease I (New England Biolabs), 5 U Lambda exonuclease (New England Biolabs), 100 U Exonuclease III (New England Biolabs), 10 U Uracil dehydrogenase (New England Biolabs) and 28% Tween20 was added to each reaction. .. Rolling circle amplification (RCA) was performed by adding 11.2 µl RCA master mix consisting of 1.07 µM of a RCA primer oligonucleotide (IDT) designed to be complementary to a sequence present in the backbone region of each DNA circle, 5.4 mM dNTP solution mix (New England Biolabs) and 20 U Inverse PCR:Article Title: Characterization of a novel gyrovirus in human stool and chicken meat Article Snippet: To sequence the complete genome of the putative novel gyrovirus, nucleic acid extracted from the original sample was subjected to rolling circle DNA amplification using Ligation:Article Title: Repressor logic modules assembled by rolling circle amplification platform to construct a set of logic gates Article Snippet: .. Circularization and RCA reaction For circularization, The 5′- and 3′-portions of the circular template (1 μM) were hybridized with a splint oligonucleotide (1 μM) in a head-to-tail way, and then were ligated by T4 DNA ligase (175 U) in ligation buffer containing 50 mM Tris pH 8.0, 10 mM MgCl2 , 5 mM DTT and 0.1 mM ATP at 16 °C for 60 min. RCA reaction solution was composed of 0.05 pmol ligated circular template, 50 mM Tris, 10 mM MgCl2 , 10 mM (NH4 )2 SO4 , 4 mM DTT (pH 7.5), SYBR Green II (1:10000) and Article Title: Imaging single DNA molecules for high precision NIPT Article Snippet: Following hybridization and ligation, 39 µl of an exonuclease master mix consisting of 20 U Exonuclease I (New England Biolabs), 5 U Lambda exonuclease (New England Biolabs), 100 U Exonuclease III (New England Biolabs), 10 U Uracil dehydrogenase (New England Biolabs) and 28% Tween20 was added to each reaction. .. Rolling circle amplification (RCA) was performed by adding 11.2 µl RCA master mix consisting of 1.07 µM of a RCA primer oligonucleotide (IDT) designed to be complementary to a sequence present in the backbone region of each DNA circle, 5.4 mM dNTP solution mix (New England Biolabs) and 20 U Indirect Immunoperoxidase Assay:Article Title: Microfluidic Exponential Rolling Circle Amplification for Sensitive microRNA Detection Directly from Biological Samples Article Snippet: other:Article Title: LEDGF/p75 interacts with divergent lentiviral integrases and modulates their enzymatic activity in vitro Article Snippet: Nicking 5′ of the reactive deoxyadenosine within one of the integrated substrate molecules prior to the treatment with phi29 DNA polymerase could explain their occurrence. Sequencing:Article Title: On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires Article Snippet: The first was described previously ( ); this method provides unbiased sequencing, especially of shorter RNA fragments that may be missed by other protocols. .. Subsequently, 127.5 μl of multiple-displacement amplification (MDA) master mix (6 μl of 25 mM dNTP mix [Roche], 15 μl of 10x Phi29 DNA polymerase reaction buffer [NEB], 7.5 μl of 1 μg/μl exonuclease-resistant random hexamers, 7.5 μl of 0.1 M dithiothreitol, 93 μl of RNase-free water) was added to each reaction, and the mixture was split into 3 tubes at 47.5 μl each and incubated at 95°C for 5 min and then at 4°C during the addition of 2.5 μl of Article Title: Characterization of a novel gyrovirus in human stool and chicken meat Article Snippet: .. To sequence the complete genome of the putative novel gyrovirus, nucleic acid extracted from the original sample was subjected to rolling circle DNA amplification using Article Title: Imaging single DNA molecules for high precision NIPT Article Snippet: .. Rolling circle amplification (RCA) was performed by adding 11.2 µl RCA master mix consisting of 1.07 µM of a RCA primer oligonucleotide (IDT) designed to be complementary to a sequence present in the backbone region of each DNA circle, 5.4 mM dNTP solution mix (New England Biolabs) and 20 U Binding Assay:Article Title: DNA amplification method tolerant to sample degradation Article Snippet: Four microliters circular DNA was mixed with 0.5 μL hexamers (400 ng/μL, Sigma) and 0.5 μL binding buffer (400 mM Tris-HCl at pH 8.0, 160 mM KCl) and denatured at 95°C for 4 min. Alternatively, the denaturation step was omitted. .. The denatured DNA was amplified using 0.3 μL DNA Extraction:Article Title: Large Circular Plasmids from Groundwater Plasmidomes Span Multiple Incompatibility Groups and Are Enriched in Multimetal Resistance Genes Article Snippet: Paragraph title: Plasmid DNA isolation optimization. ... The DNA was then amplified with Marker:Article Title: Dual functional Phi29 DNA polymerase-triggered exponential rolling circle amplification for sequence-specific detection of target DNA embedded in long-stranded genomic DNA Article Snippet: T4 DNA ligase, Article Title: Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures Article Snippet: DNA marker, T4 DNA ligase, Exonuclease I, Exonuclease III, RiboLock RNase Inhibitor and Trizol Reagent were obtained from Takara Biotechnology Co. Ltd (Dalian, China). .. Poly(U) polymerase, RNA Sequencing Assay:Article Title: On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires Article Snippet: Paragraph title: RNA sequencing of Spirulina samples. ... Subsequently, 127.5 μl of multiple-displacement amplification (MDA) master mix (6 μl of 25 mM dNTP mix [Roche], 15 μl of 10x Phi29 DNA polymerase reaction buffer [NEB], 7.5 μl of 1 μg/μl exonuclease-resistant random hexamers, 7.5 μl of 0.1 M dithiothreitol, 93 μl of RNase-free water) was added to each reaction, and the mixture was split into 3 tubes at 47.5 μl each and incubated at 95°C for 5 min and then at 4°C during the addition of 2.5 μl of Multiple Displacement Amplification:Article Title: Development of an easy and cost-effective method for non-invasive genotyping of insects Article Snippet: .. The MDA reaction was carried out in a 20 μl final volume containing 2 μl 10× reaction buffer with 1.6 μl dNTP mix (2.5 μM each), 0.5 μl Article Title: On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires Article Snippet: .. Subsequently, 127.5 μl of multiple-displacement amplification (MDA) master mix (6 μl of 25 mM dNTP mix [Roche], 15 μl of 10x Labeling:Article Title: Imaging single DNA molecules for high precision NIPT Article Snippet: Rolling circle amplification (RCA) was performed by adding 11.2 µl RCA master mix consisting of 1.07 µM of a RCA primer oligonucleotide (IDT) designed to be complementary to a sequence present in the backbone region of each DNA circle, 5.4 mM dNTP solution mix (New England Biolabs) and 20 U Purification:Article Title: Development of an easy and cost-effective method for non-invasive genotyping of insects Article Snippet: The MDA reaction was carried out in a 20 μl final volume containing 2 μl 10× reaction buffer with 1.6 μl dNTP mix (2.5 μM each), 0.5 μl Article Title: On the Origin of Reverse Transcriptase-Using CRISPR-Cas Systems and Their Hyperdiverse, Enigmatic Spacer Repertoires Article Snippet: Subsequently, 127.5 μl of multiple-displacement amplification (MDA) master mix (6 μl of 25 mM dNTP mix [Roche], 15 μl of 10x Phi29 DNA polymerase reaction buffer [NEB], 7.5 μl of 1 μg/μl exonuclease-resistant random hexamers, 7.5 μl of 0.1 M dithiothreitol, 93 μl of RNase-free water) was added to each reaction, and the mixture was split into 3 tubes at 47.5 μl each and incubated at 95°C for 5 min and then at 4°C during the addition of 2.5 μl of Article Title: DNA amplification method tolerant to sample degradation Article Snippet: The fragmented DNAs were circularized with 0.5 μL T4 DNA ligase (2000 units/μL, New England Biolabs) in a volume of 15 μL at room temperature for 2 h. After inactivation of ligase at 65°C for 10 min, linear DNAs were eliminated with 1.2 μL Lamda Exonuclease (5 units/μL, New England Biolabs) and 0.3 μL Exonuclease I (20 units/μL, New England Biolabs) in a volume of 25 μL at 37°C for 1 h. The circularized DNAs were then purified using a QIAquick PCR Purification Kit (QIAGEN) and eluted in 35 μL of H2 O. .. The denatured DNA was amplified using 0.3 μL Article Title: Whole genome sequencing of amplified Plasmodium knowlesi DNA from unprocessed blood reveals genetic exchange events between Malaysian Peninsular and Borneo subpopulations Article Snippet: .. SWGA reactions were performed containing a maximum of 50 ng of total input genomic DNA (and a minimum of 5 ng), 5 µl of 10 x Article Title: Microfluidic Exponential Rolling Circle Amplification for Sensitive microRNA Detection Directly from Biological Samples Article Snippet: Synthetic oligonucleotides and miRNAs were synthesized and purified with polyacrylamide gel electrophoresis (PAGE) by Integrated DNA Technologies (IDT, Coralville, IA). .. Polymerase Chain Reaction:Article Title: Large Circular Plasmids from Groundwater Plasmidomes Span Multiple Incompatibility Groups and Are Enriched in Multimetal Resistance Genes Article Snippet: If 16S rRNA PCR product was visible on a 1% agarose gel, another overnight digestion reaction was performed until the product could no longer be visualized. .. The DNA was then amplified with Article Title: DNA amplification method tolerant to sample degradation Article Snippet: The fragmented DNAs were circularized with 0.5 μL T4 DNA ligase (2000 units/μL, New England Biolabs) in a volume of 15 μL at room temperature for 2 h. After inactivation of ligase at 65°C for 10 min, linear DNAs were eliminated with 1.2 μL Lamda Exonuclease (5 units/μL, New England Biolabs) and 0.3 μL Exonuclease I (20 units/μL, New England Biolabs) in a volume of 25 μL at 37°C for 1 h. The circularized DNAs were then purified using a QIAquick PCR Purification Kit (QIAGEN) and eluted in 35 μL of H2 O. .. The denatured DNA was amplified using 0.3 μL Article Title: Whole genome sequencing of amplified Plasmodium knowlesi DNA from unprocessed blood reveals genetic exchange events between Malaysian Peninsular and Borneo subpopulations Article Snippet: All SWGA reactions were carried out in the UV Cabinet for PCR Operations (UV-B-AR, Grant-Bio) to minimize contamination. .. SWGA reactions were performed containing a maximum of 50 ng of total input genomic DNA (and a minimum of 5 ng), 5 µl of 10 x Polyacrylamide Gel Electrophoresis:Article Title: Microfluidic Exponential Rolling Circle Amplification for Sensitive microRNA Detection Directly from Biological Samples Article Snippet: Synthetic oligonucleotides and miRNAs were synthesized and purified with polyacrylamide gel electrophoresis (PAGE) by Integrated DNA Technologies (IDT, Coralville, IA). .. Plasmid Preparation:Article Title: Large Circular Plasmids from Groundwater Plasmidomes Span Multiple Incompatibility Groups and Are Enriched in Multimetal Resistance Genes Article Snippet: Paragraph title: Plasmid DNA isolation optimization. ... The DNA was then amplified with Software:Article Title: Dual functional Phi29 DNA polymerase-triggered exponential rolling circle amplification for sequence-specific detection of target DNA embedded in long-stranded genomic DNA Article Snippet: Molar extinction coefficient was determined by OligoAnayzer 3.1 software provided in the following website ( http://sg.idtdna.com/calc/analyzer ). .. T4 DNA ligase, Real-time Polymerase Chain Reaction:Article Title: Large Circular Plasmids from Groundwater Plasmidomes Span Multiple Incompatibility Groups and Are Enriched in Multimetal Resistance Genes Article Snippet: The DNA was then amplified with Agarose Gel Electrophoresis:Article Title: Large Circular Plasmids from Groundwater Plasmidomes Span Multiple Incompatibility Groups and Are Enriched in Multimetal Resistance Genes Article Snippet: If 16S rRNA PCR product was visible on a 1% agarose gel, another overnight digestion reaction was performed until the product could no longer be visualized. .. The DNA was then amplified with Next-Generation Sequencing:Article Title: Characterization of a novel gyrovirus in human stool and chicken meat Article Snippet: Roche 454 high throughput sequencing was applied to nucleic acid extracted individually from 96 faecal samples from patients with diarrhoea from whom no other viral pathogen had been identified. .. To sequence the complete genome of the putative novel gyrovirus, nucleic acid extracted from the original sample was subjected to rolling circle DNA amplification using Concentration Assay:Article Title: Rolling Circle Amplification, a Powerful Tool for Genetic and Functional Studies of Complete Hepatitis B Virus Genomes from Low-Level Infections and for Directly Probing Covalently Closed Circular DNA ▿ Article Snippet: .. Sample mixtures were combined with 10 μl of reaction mixture containing: 1× Phi29 buffer, the eight primers at a concentration of 0.5 μM each, 0.4 mg/ml of bovine serum albumin, 4 mM of dithiothreitol, 2 mM of each dNTP, and 10 U of the Article Title: A novel method for detection of HBVcccDNA in hepatocytes using rolling circle amplification combined with in situ PCR Article Snippet: .. In order to increase the efficiency of amplification, RCA was divided into two steps: First-step, 14 μl primer combining reaction mixture containing primers at a operative solutionconcentration of 0.5 μmol/l each , 2 μl reaction buffer and 11 μl ddH2 O were loaded onto the tissue sections on glass slides and sealed with rubber cement, followed by heating at 95°C for 6 min, and then placed on ice for 15 min. Second-step the RCA amplification was as follows: 23 μl of reaction mixture was added to tissue slides containing primers at a operative solution concentration of 0.5 μmol/l each, 10 mg/ml bovine serum albumin (BSA), 0.32 mmol/l of dNTP, 20 U of the High Throughput Screening Assay:Article Title: Characterization of a novel gyrovirus in human stool and chicken meat Article Snippet: Paragraph title: Viral genetic detection and characterization by high-throughput deep-sequencing methods ... To sequence the complete genome of the putative novel gyrovirus, nucleic acid extracted from the original sample was subjected to rolling circle DNA amplification using |
