restriction endonuclease nla iii  (New England Biolabs)


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    New England Biolabs restriction endonuclease nla iii
    Restriction Endonuclease Nla Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    restriction endonuclease nla iii  (New England Biolabs)


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    New England Biolabs restriction endonuclease nla iii
    Restriction Endonuclease Nla Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hae iii restriction endonuclease  (New England Biolabs)


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    New England Biolabs hae iii restriction endonuclease
    Hae Iii Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    restriction endonuclease nla iii  (New England Biolabs)


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    New England Biolabs restriction endonuclease nla iii
    Restriction Endonuclease Nla Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hind iii restriction endonuclease  (New England Biolabs)


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    New England Biolabs hind iii restriction endonuclease
    Characterization of digested BAC vector and PFGE size fractionation of digested HMW by Hind <t>III.</t> (a) Characterization of digested vector by electrophoresis; M: 1 <t>kb</t> <t>DNA</t> ladder; 1: BAC vector digested by Hind III; 2: BAC vector digested by B am H I and X ho I; (b) initial PFGE size fractionation of partially digested HMW; M: Lamda Ladder PFG Marker; 1, 5, 10, 15, 20, and 50 U/ μ gDNA, respectively; (c) second-size selection PFGE, used to eliminate smaller DNA molecules; M: Lamda Ladder PFG Marker; 100–200 kb, 200–300 kb, 300–400 kb, respectively; (d) quantification of Wuzhishan miniature pig genomic DNA.1: 100–200 kb DNA; 2: 200–300 kb DNA; 3: 300–400 kb DNA.
    Hind Iii Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Construction and Preliminary Characterization Analysis of Wuzhishan Miniature Pig Bacterial Artificial Chromosome Library with Approximately 8-Fold Genome Equivalent Coverage"

    Article Title: Construction and Preliminary Characterization Analysis of Wuzhishan Miniature Pig Bacterial Artificial Chromosome Library with Approximately 8-Fold Genome Equivalent Coverage

    Journal: BioMed Research International

    doi: 10.1155/2013/587493

    Characterization of digested BAC vector and PFGE size fractionation of digested HMW by Hind III. (a) Characterization of digested vector by electrophoresis; M: 1 kb DNA ladder; 1: BAC vector digested by Hind III; 2: BAC vector digested by B am H I and X ho I; (b) initial PFGE size fractionation of partially digested HMW; M: Lamda Ladder PFG Marker; 1, 5, 10, 15, 20, and 50 U/ μ gDNA, respectively; (c) second-size selection PFGE, used to eliminate smaller DNA molecules; M: Lamda Ladder PFG Marker; 100–200 kb, 200–300 kb, 300–400 kb, respectively; (d) quantification of Wuzhishan miniature pig genomic DNA.1: 100–200 kb DNA; 2: 200–300 kb DNA; 3: 300–400 kb DNA.
    Figure Legend Snippet: Characterization of digested BAC vector and PFGE size fractionation of digested HMW by Hind III. (a) Characterization of digested vector by electrophoresis; M: 1 kb DNA ladder; 1: BAC vector digested by Hind III; 2: BAC vector digested by B am H I and X ho I; (b) initial PFGE size fractionation of partially digested HMW; M: Lamda Ladder PFG Marker; 1, 5, 10, 15, 20, and 50 U/ μ gDNA, respectively; (c) second-size selection PFGE, used to eliminate smaller DNA molecules; M: Lamda Ladder PFG Marker; 100–200 kb, 200–300 kb, 300–400 kb, respectively; (d) quantification of Wuzhishan miniature pig genomic DNA.1: 100–200 kb DNA; 2: 200–300 kb DNA; 3: 300–400 kb DNA.

    Techniques Used: Plasmid Preparation, Fractionation, Electrophoresis, Marker, Selection

    Summary of the Wuzhishan miniature pig BAC library.
    Figure Legend Snippet: Summary of the Wuzhishan miniature pig BAC library.

    Techniques Used: Clone Assay

    restriction endonuclease nla iii  (New England Biolabs)


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    New England Biolabs restriction endonuclease nla iii
    Restriction Endonuclease Nla Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    restriction endonuclease nla iii  (New England Biolabs)


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    New England Biolabs restriction endonuclease nla iii
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    endonuclease iii nth  (New England Biolabs)


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    New England Biolabs endonuclease iii nth
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    endo iii  (New England Biolabs)


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    New England Biolabs endo iii
    ( A ) Outline of the CD-seq and enzymatic cleavage methods used to map oxidized thymines at single-base resolution. ( B ) Base resolution view of oxidized thymines along the human NOD1 and CFAP77 genes. Orange and lavender segments represent the divergent read pairs. Green arrows indicate single-base gaps matching the excised oxidized DNA base. ( C ) Heatmaps and metagene profiles of permanganate-oxidized thymines along human genes. DNA from untreated cells produced very few divergent read pairs, and the data could not be used in similar displays. Different cleavage enzymes, <t>Endo</t> <t>III</t> or NEIL1, or a combination of the two was used.
    Endo Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Concordance of hydrogen peroxide–induced 8-oxo-guanine patterns with two cancer mutation signatures of upper GI tract tumors"

    Article Title: Concordance of hydrogen peroxide–induced 8-oxo-guanine patterns with two cancer mutation signatures of upper GI tract tumors

    Journal: Science Advances

    doi: 10.1126/sciadv.abn3815

    ( A ) Outline of the CD-seq and enzymatic cleavage methods used to map oxidized thymines at single-base resolution. ( B ) Base resolution view of oxidized thymines along the human NOD1 and CFAP77 genes. Orange and lavender segments represent the divergent read pairs. Green arrows indicate single-base gaps matching the excised oxidized DNA base. ( C ) Heatmaps and metagene profiles of permanganate-oxidized thymines along human genes. DNA from untreated cells produced very few divergent read pairs, and the data could not be used in similar displays. Different cleavage enzymes, Endo III or NEIL1, or a combination of the two was used.
    Figure Legend Snippet: ( A ) Outline of the CD-seq and enzymatic cleavage methods used to map oxidized thymines at single-base resolution. ( B ) Base resolution view of oxidized thymines along the human NOD1 and CFAP77 genes. Orange and lavender segments represent the divergent read pairs. Green arrows indicate single-base gaps matching the excised oxidized DNA base. ( C ) Heatmaps and metagene profiles of permanganate-oxidized thymines along human genes. DNA from untreated cells produced very few divergent read pairs, and the data could not be used in similar displays. Different cleavage enzymes, Endo III or NEIL1, or a combination of the two was used.

    Techniques Used: Produced

    ( A ) Trinucleotide sequence specificity. Forty-eight trinucleotide contexts were analyzed, but the gapped reads occurred chiefly at trinucleotides with central thymine. Different enzymes (Endo III and NEIL1 in combination and alone) were used to excise the oxidized bases. ( B ) Sequence context of thymine oxidation by permanganate using logo plot analysis. Position 11 represents the permanganate-oxidized thymine base. At each base position, the height of each letter represents the relative frequency of that nucleic acid base. Note the enrichment of A or C 3′ to the thymine.
    Figure Legend Snippet: ( A ) Trinucleotide sequence specificity. Forty-eight trinucleotide contexts were analyzed, but the gapped reads occurred chiefly at trinucleotides with central thymine. Different enzymes (Endo III and NEIL1 in combination and alone) were used to excise the oxidized bases. ( B ) Sequence context of thymine oxidation by permanganate using logo plot analysis. Position 11 represents the permanganate-oxidized thymine base. At each base position, the height of each letter represents the relative frequency of that nucleic acid base. Note the enrichment of A or C 3′ to the thymine.

    Techniques Used: Sequencing

    endo iii  (New England Biolabs)


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    New England Biolabs endo iii
    ( A ) Outline of the CD-seq and enzymatic cleavage methods used to map oxidized thymines at single-base resolution. ( B ) Base resolution view of oxidized thymines along the human NOD1 and CFAP77 genes. Orange and lavender segments represent the divergent read pairs. Green arrows indicate single-base gaps matching the excised oxidized DNA base. ( C ) Heatmaps and metagene profiles of permanganate-oxidized thymines along human genes. DNA from untreated cells produced very few divergent read pairs, and the data could not be used in similar displays. Different cleavage enzymes, <t>Endo</t> <t>III</t> or NEIL1, or a combination of the two was used.
    Endo Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Concordance of hydrogen peroxide–induced 8-oxo-guanine patterns with two cancer mutation signatures of upper GI tract tumors"

    Article Title: Concordance of hydrogen peroxide–induced 8-oxo-guanine patterns with two cancer mutation signatures of upper GI tract tumors

    Journal: Science Advances

    doi: 10.1126/sciadv.abn3815

    ( A ) Outline of the CD-seq and enzymatic cleavage methods used to map oxidized thymines at single-base resolution. ( B ) Base resolution view of oxidized thymines along the human NOD1 and CFAP77 genes. Orange and lavender segments represent the divergent read pairs. Green arrows indicate single-base gaps matching the excised oxidized DNA base. ( C ) Heatmaps and metagene profiles of permanganate-oxidized thymines along human genes. DNA from untreated cells produced very few divergent read pairs, and the data could not be used in similar displays. Different cleavage enzymes, Endo III or NEIL1, or a combination of the two was used.
    Figure Legend Snippet: ( A ) Outline of the CD-seq and enzymatic cleavage methods used to map oxidized thymines at single-base resolution. ( B ) Base resolution view of oxidized thymines along the human NOD1 and CFAP77 genes. Orange and lavender segments represent the divergent read pairs. Green arrows indicate single-base gaps matching the excised oxidized DNA base. ( C ) Heatmaps and metagene profiles of permanganate-oxidized thymines along human genes. DNA from untreated cells produced very few divergent read pairs, and the data could not be used in similar displays. Different cleavage enzymes, Endo III or NEIL1, or a combination of the two was used.

    Techniques Used: Produced

    ( A ) Trinucleotide sequence specificity. Forty-eight trinucleotide contexts were analyzed, but the gapped reads occurred chiefly at trinucleotides with central thymine. Different enzymes (Endo III and NEIL1 in combination and alone) were used to excise the oxidized bases. ( B ) Sequence context of thymine oxidation by permanganate using logo plot analysis. Position 11 represents the permanganate-oxidized thymine base. At each base position, the height of each letter represents the relative frequency of that nucleic acid base. Note the enrichment of A or C 3′ to the thymine.
    Figure Legend Snippet: ( A ) Trinucleotide sequence specificity. Forty-eight trinucleotide contexts were analyzed, but the gapped reads occurred chiefly at trinucleotides with central thymine. Different enzymes (Endo III and NEIL1 in combination and alone) were used to excise the oxidized bases. ( B ) Sequence context of thymine oxidation by permanganate using logo plot analysis. Position 11 represents the permanganate-oxidized thymine base. At each base position, the height of each letter represents the relative frequency of that nucleic acid base. Note the enrichment of A or C 3′ to the thymine.

    Techniques Used: Sequencing

    endonuclease iii  (New England Biolabs)


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    New England Biolabs endonuclease iii
    Oxidative damage to pyrimidines <t>in</t> <t>DNA.</t> Oxidative damage to DNA pyrimidines in human PBMCs treated with TBBPA, TBBPS, 2,4,6-TBP and PBP at the concentrations of 0.01 µg/mL, 0.1 µg/mL and 1 µg/mL for 24 h. DNA damage was measured as the percentage of DNA in the comet tail using the enzyme endo <t>III</t> and the alkaline version of the comet assay. The mean ± SD was calculated for 3 experiments (3 blood donors). Statistically different from negative control at *P<0.05. Statistical analysis was conducted using one-way ANOVA and a posteriori Tukey test.
    Endonuclease Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Genotoxic Mechanism of Action of TBBPA, TBBPS and Selected Bromophenols in Human Peripheral Blood Mononuclear Cells"

    Article Title: Genotoxic Mechanism of Action of TBBPA, TBBPS and Selected Bromophenols in Human Peripheral Blood Mononuclear Cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.869741

    Oxidative damage to pyrimidines in DNA. Oxidative damage to DNA pyrimidines in human PBMCs treated with TBBPA, TBBPS, 2,4,6-TBP and PBP at the concentrations of 0.01 µg/mL, 0.1 µg/mL and 1 µg/mL for 24 h. DNA damage was measured as the percentage of DNA in the comet tail using the enzyme endo III and the alkaline version of the comet assay. The mean ± SD was calculated for 3 experiments (3 blood donors). Statistically different from negative control at *P<0.05. Statistical analysis was conducted using one-way ANOVA and a posteriori Tukey test.
    Figure Legend Snippet: Oxidative damage to pyrimidines in DNA. Oxidative damage to DNA pyrimidines in human PBMCs treated with TBBPA, TBBPS, 2,4,6-TBP and PBP at the concentrations of 0.01 µg/mL, 0.1 µg/mL and 1 µg/mL for 24 h. DNA damage was measured as the percentage of DNA in the comet tail using the enzyme endo III and the alkaline version of the comet assay. The mean ± SD was calculated for 3 experiments (3 blood donors). Statistically different from negative control at *P<0.05. Statistical analysis was conducted using one-way ANOVA and a posteriori Tukey test.

    Techniques Used: Single Cell Gel Electrophoresis, Negative Control

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    New England Biolabs restriction endonuclease nla iii
    Restriction Endonuclease Nla Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs hae iii restriction endonuclease
    Hae Iii Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs hind iii restriction endonuclease
    Characterization of digested BAC vector and PFGE size fractionation of digested HMW by Hind <t>III.</t> (a) Characterization of digested vector by electrophoresis; M: 1 <t>kb</t> <t>DNA</t> ladder; 1: BAC vector digested by Hind III; 2: BAC vector digested by B am H I and X ho I; (b) initial PFGE size fractionation of partially digested HMW; M: Lamda Ladder PFG Marker; 1, 5, 10, 15, 20, and 50 U/ μ gDNA, respectively; (c) second-size selection PFGE, used to eliminate smaller DNA molecules; M: Lamda Ladder PFG Marker; 100–200 kb, 200–300 kb, 300–400 kb, respectively; (d) quantification of Wuzhishan miniature pig genomic DNA.1: 100–200 kb DNA; 2: 200–300 kb DNA; 3: 300–400 kb DNA.
    Hind Iii Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs endonuclease iii nth
    Characterization of digested BAC vector and PFGE size fractionation of digested HMW by Hind <t>III.</t> (a) Characterization of digested vector by electrophoresis; M: 1 <t>kb</t> <t>DNA</t> ladder; 1: BAC vector digested by Hind III; 2: BAC vector digested by B am H I and X ho I; (b) initial PFGE size fractionation of partially digested HMW; M: Lamda Ladder PFG Marker; 1, 5, 10, 15, 20, and 50 U/ μ gDNA, respectively; (c) second-size selection PFGE, used to eliminate smaller DNA molecules; M: Lamda Ladder PFG Marker; 100–200 kb, 200–300 kb, 300–400 kb, respectively; (d) quantification of Wuzhishan miniature pig genomic DNA.1: 100–200 kb DNA; 2: 200–300 kb DNA; 3: 300–400 kb DNA.
    Endonuclease Iii Nth, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs endo iii
    ( A ) Outline of the CD-seq and enzymatic cleavage methods used to map oxidized thymines at single-base resolution. ( B ) Base resolution view of oxidized thymines along the human NOD1 and CFAP77 genes. Orange and lavender segments represent the divergent read pairs. Green arrows indicate single-base gaps matching the excised oxidized DNA base. ( C ) Heatmaps and metagene profiles of permanganate-oxidized thymines along human genes. DNA from untreated cells produced very few divergent read pairs, and the data could not be used in similar displays. Different cleavage enzymes, <t>Endo</t> <t>III</t> or NEIL1, or a combination of the two was used.
    Endo Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/endo iii/product/New England Biolabs
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    New England Biolabs endonuclease iii
    Oxidative damage to pyrimidines <t>in</t> <t>DNA.</t> Oxidative damage to DNA pyrimidines in human PBMCs treated with TBBPA, TBBPS, 2,4,6-TBP and PBP at the concentrations of 0.01 µg/mL, 0.1 µg/mL and 1 µg/mL for 24 h. DNA damage was measured as the percentage of DNA in the comet tail using the enzyme endo <t>III</t> and the alkaline version of the comet assay. The mean ± SD was calculated for 3 experiments (3 blood donors). Statistically different from negative control at *P<0.05. Statistical analysis was conducted using one-way ANOVA and a posteriori Tukey test.
    Endonuclease Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Characterization of digested BAC vector and PFGE size fractionation of digested HMW by Hind III. (a) Characterization of digested vector by electrophoresis; M: 1 kb DNA ladder; 1: BAC vector digested by Hind III; 2: BAC vector digested by B am H I and X ho I; (b) initial PFGE size fractionation of partially digested HMW; M: Lamda Ladder PFG Marker; 1, 5, 10, 15, 20, and 50 U/ μ gDNA, respectively; (c) second-size selection PFGE, used to eliminate smaller DNA molecules; M: Lamda Ladder PFG Marker; 100–200 kb, 200–300 kb, 300–400 kb, respectively; (d) quantification of Wuzhishan miniature pig genomic DNA.1: 100–200 kb DNA; 2: 200–300 kb DNA; 3: 300–400 kb DNA.

    Journal: BioMed Research International

    Article Title: Construction and Preliminary Characterization Analysis of Wuzhishan Miniature Pig Bacterial Artificial Chromosome Library with Approximately 8-Fold Genome Equivalent Coverage

    doi: 10.1155/2013/587493

    Figure Lengend Snippet: Characterization of digested BAC vector and PFGE size fractionation of digested HMW by Hind III. (a) Characterization of digested vector by electrophoresis; M: 1 kb DNA ladder; 1: BAC vector digested by Hind III; 2: BAC vector digested by B am H I and X ho I; (b) initial PFGE size fractionation of partially digested HMW; M: Lamda Ladder PFG Marker; 1, 5, 10, 15, 20, and 50 U/ μ gDNA, respectively; (c) second-size selection PFGE, used to eliminate smaller DNA molecules; M: Lamda Ladder PFG Marker; 100–200 kb, 200–300 kb, 300–400 kb, respectively; (d) quantification of Wuzhishan miniature pig genomic DNA.1: 100–200 kb DNA; 2: 200–300 kb DNA; 3: 300–400 kb DNA.

    Article Snippet: After establishing the minimal amount of restriction enzyme required for complete digestion of the vector in a series of test reactions, the vector DNA was digested with an appropriate amount of Hind III restriction endonuclease (New England Biolabs) and treated with alkaline phosphatase (CIAP) for dephosphorylation [ ].

    Techniques: Plasmid Preparation, Fractionation, Electrophoresis, Marker, Selection

    Summary of the Wuzhishan miniature pig BAC library.

    Journal: BioMed Research International

    Article Title: Construction and Preliminary Characterization Analysis of Wuzhishan Miniature Pig Bacterial Artificial Chromosome Library with Approximately 8-Fold Genome Equivalent Coverage

    doi: 10.1155/2013/587493

    Figure Lengend Snippet: Summary of the Wuzhishan miniature pig BAC library.

    Article Snippet: After establishing the minimal amount of restriction enzyme required for complete digestion of the vector in a series of test reactions, the vector DNA was digested with an appropriate amount of Hind III restriction endonuclease (New England Biolabs) and treated with alkaline phosphatase (CIAP) for dephosphorylation [ ].

    Techniques: Clone Assay

    ( A ) Outline of the CD-seq and enzymatic cleavage methods used to map oxidized thymines at single-base resolution. ( B ) Base resolution view of oxidized thymines along the human NOD1 and CFAP77 genes. Orange and lavender segments represent the divergent read pairs. Green arrows indicate single-base gaps matching the excised oxidized DNA base. ( C ) Heatmaps and metagene profiles of permanganate-oxidized thymines along human genes. DNA from untreated cells produced very few divergent read pairs, and the data could not be used in similar displays. Different cleavage enzymes, Endo III or NEIL1, or a combination of the two was used.

    Journal: Science Advances

    Article Title: Concordance of hydrogen peroxide–induced 8-oxo-guanine patterns with two cancer mutation signatures of upper GI tract tumors

    doi: 10.1126/sciadv.abn3815

    Figure Lengend Snippet: ( A ) Outline of the CD-seq and enzymatic cleavage methods used to map oxidized thymines at single-base resolution. ( B ) Base resolution view of oxidized thymines along the human NOD1 and CFAP77 genes. Orange and lavender segments represent the divergent read pairs. Green arrows indicate single-base gaps matching the excised oxidized DNA base. ( C ) Heatmaps and metagene profiles of permanganate-oxidized thymines along human genes. DNA from untreated cells produced very few divergent read pairs, and the data could not be used in similar displays. Different cleavage enzymes, Endo III or NEIL1, or a combination of the two was used.

    Article Snippet: DNA base damage was evaluated by treatment with Endo III (NEB) and/or NEIL1 (OriGene, Rockville, MD) DNA glycosylases, followed by Endo IV and S1 treatment, and then observed on a 1% agarose gel.

    Techniques: Produced

    ( A ) Trinucleotide sequence specificity. Forty-eight trinucleotide contexts were analyzed, but the gapped reads occurred chiefly at trinucleotides with central thymine. Different enzymes (Endo III and NEIL1 in combination and alone) were used to excise the oxidized bases. ( B ) Sequence context of thymine oxidation by permanganate using logo plot analysis. Position 11 represents the permanganate-oxidized thymine base. At each base position, the height of each letter represents the relative frequency of that nucleic acid base. Note the enrichment of A or C 3′ to the thymine.

    Journal: Science Advances

    Article Title: Concordance of hydrogen peroxide–induced 8-oxo-guanine patterns with two cancer mutation signatures of upper GI tract tumors

    doi: 10.1126/sciadv.abn3815

    Figure Lengend Snippet: ( A ) Trinucleotide sequence specificity. Forty-eight trinucleotide contexts were analyzed, but the gapped reads occurred chiefly at trinucleotides with central thymine. Different enzymes (Endo III and NEIL1 in combination and alone) were used to excise the oxidized bases. ( B ) Sequence context of thymine oxidation by permanganate using logo plot analysis. Position 11 represents the permanganate-oxidized thymine base. At each base position, the height of each letter represents the relative frequency of that nucleic acid base. Note the enrichment of A or C 3′ to the thymine.

    Article Snippet: DNA base damage was evaluated by treatment with Endo III (NEB) and/or NEIL1 (OriGene, Rockville, MD) DNA glycosylases, followed by Endo IV and S1 treatment, and then observed on a 1% agarose gel.

    Techniques: Sequencing

    Oxidative damage to pyrimidines in DNA. Oxidative damage to DNA pyrimidines in human PBMCs treated with TBBPA, TBBPS, 2,4,6-TBP and PBP at the concentrations of 0.01 µg/mL, 0.1 µg/mL and 1 µg/mL for 24 h. DNA damage was measured as the percentage of DNA in the comet tail using the enzyme endo III and the alkaline version of the comet assay. The mean ± SD was calculated for 3 experiments (3 blood donors). Statistically different from negative control at *P<0.05. Statistical analysis was conducted using one-way ANOVA and a posteriori Tukey test.

    Journal: Frontiers in Immunology

    Article Title: Genotoxic Mechanism of Action of TBBPA, TBBPS and Selected Bromophenols in Human Peripheral Blood Mononuclear Cells

    doi: 10.3389/fimmu.2022.869741

    Figure Lengend Snippet: Oxidative damage to pyrimidines in DNA. Oxidative damage to DNA pyrimidines in human PBMCs treated with TBBPA, TBBPS, 2,4,6-TBP and PBP at the concentrations of 0.01 µg/mL, 0.1 µg/mL and 1 µg/mL for 24 h. DNA damage was measured as the percentage of DNA in the comet tail using the enzyme endo III and the alkaline version of the comet assay. The mean ± SD was calculated for 3 experiments (3 blood donors). Statistically different from negative control at *P<0.05. Statistical analysis was conducted using one-way ANOVA and a posteriori Tukey test.

    Article Snippet: Endonuclease III and human 8-oxoguanine DNA glycosylase were bought in New England BioLabs (USA).

    Techniques: Single Cell Gel Electrophoresis, Negative Control