deep vent polymerase  (New England Biolabs)


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    Name:
    Deep Vent exo DNA Polymerase
    Description:
    Deep Vent exo DNA Polymerase 1 000 units
    Catalog Number:
    m0259l
    Price:
    414
    Size:
    1 000 units
    Category:
    Thermostable DNA Polymerases
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    Structured Review

    New England Biolabs deep vent polymerase
    Deep Vent exo DNA Polymerase
    Deep Vent exo DNA Polymerase 1 000 units
    https://www.bioz.com/result/deep vent polymerase/product/New England Biolabs
    Average 94 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    deep vent polymerase - by Bioz Stars, 2021-02
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: FcRn mediates elongated serum half-life of human IgG in cattle.
    Article Snippet: .. MethodsExpression and purification of the soluble bovine FcRn The soluble bovine FcRn (sbFcRn) heavy-chain cDNA (116– 982 bp; GenBank AF139106) was PCR amplified with Deep Vent polymerase (New England Biolab, Beverly, MA, USA) from a eucaryotic expression construct described before (29). .. The forward primer contains an EcoRI site, a ‘Kozak’ sequence (italic) and the coding region (underlined) (bFcRnBACU_for: 59-ATC AGA ATT CCC TATAAATAT GCG GCT TCC CCG GCC T39); the reverse primer contains the gene-specific coding re- gions (underlined), a factor Xa site (italic), a 63His tag coding sequence, a stop codon and an EcoRI site (bFcRnBACU_rev: 59-ATC AGA ATT CCT AAT GAT GAT GAT GAT GAT GAC GAC CTT CGA TCA GCT CCA CCG TGA GGG GCT-39).

    Article Title: Systematic evaluation of genome-wide methylated DNA enrichment using a CpG island array
    Article Snippet: .. PCR amplification of each enriched DNA fragment was performed in a 300 ul volume mixture comprised of the restriction DNA product, 10× ThermoPol Buffer, 2.5 uM H-24 primer, 0.2 mM dNTP mixture, and 12 U Deep Vent(exo-) DNA polymerase (New England Biolabs, USA). .. The amplification conditions were 5 min at 72°C to fill in the protruding ends of the ligated DNA fragments, followed by 20 cycles (1 min at 97°C and 3 min at 72°C), with a final extension for 10 min at 72°C.

    Article Title: Glycoproteins M and N of Pseudorabies Virus Form a Disulfide-Linked Complex
    Article Snippet: .. The UL49.5 gene was amplified by PCR with Deep Vent polymerase (New England Biolabs, Schwalbach, Germany) and the following primers: gN-1, 5′-TGGATCCAGGATGGTCTCCTCCG-3′ (positions 365 to 377 of GenBank accession no. ), and gN-2, 5′-CGAATTCGATCTTTATACGCGCCGCC-3′ (positions 82 to 62). .. By using the Bam HI and Eco RI restriction sites contained in the primers, the PCR product was inserted into pVL1393 (PharMingen, San Diego, Calif.) under the control of the polyhedrin promoter, resulting in plasmid pVL1393gN.

    Mutagenesis:

    Article Title: Identification of a Human Papillomavirus Type 16-Specific Epitope on the C-Terminal Arm of the Major Capsid Protein L1
    Article Snippet: .. Deep Vent polymerase (New England Biolabs, Beverly, Mass.) was used for high fidelity PCR, PFU turbo (Stratagene La Jolla, Calif.) was used for mutagenesis, and Taq (Invitrogen) was for analysis of recombinant bacmids. .. The L1 amplimers were cloned into the Gateway pDonr201 vector and from there into the pDest8 vector with Gateway technology following the manufacturer's protocol (Invitrogen).

    Electrophoretic Mobility Shift Assay:

    Article Title: Selective Glutaraldehyde-Mediated Coupling of Proteins to the 3′-Adenine Terminus of Polymerase Chain Reaction Products
    Article Snippet: .. It is of interest to note that this band shift occurred after using Deep Vent (exo-) polymerase which, according to the manufacturer, is thought to produce 3′ adenine overhangs in only 30% of its PCR products. .. Even with this reduced abundance of 3′-adenine overhangs (versus nearly 100% 3′-adenine addition with Taq ), we were still able to detect a gel shift indicative of a DNA–protein conjugate in lane 7 of .

    Purification:

    Article Title: FcRn mediates elongated serum half-life of human IgG in cattle.
    Article Snippet: .. MethodsExpression and purification of the soluble bovine FcRn The soluble bovine FcRn (sbFcRn) heavy-chain cDNA (116– 982 bp; GenBank AF139106) was PCR amplified with Deep Vent polymerase (New England Biolab, Beverly, MA, USA) from a eucaryotic expression construct described before (29). .. The forward primer contains an EcoRI site, a ‘Kozak’ sequence (italic) and the coding region (underlined) (bFcRnBACU_for: 59-ATC AGA ATT CCC TATAAATAT GCG GCT TCC CCG GCC T39); the reverse primer contains the gene-specific coding re- gions (underlined), a factor Xa site (italic), a 63His tag coding sequence, a stop codon and an EcoRI site (bFcRnBACU_rev: 59-ATC AGA ATT CCT AAT GAT GAT GAT GAT GAT GAC GAC CTT CGA TCA GCT CCA CCG TGA GGG GCT-39).

    Construct:

    Article Title: FcRn mediates elongated serum half-life of human IgG in cattle.
    Article Snippet: .. MethodsExpression and purification of the soluble bovine FcRn The soluble bovine FcRn (sbFcRn) heavy-chain cDNA (116– 982 bp; GenBank AF139106) was PCR amplified with Deep Vent polymerase (New England Biolab, Beverly, MA, USA) from a eucaryotic expression construct described before (29). .. The forward primer contains an EcoRI site, a ‘Kozak’ sequence (italic) and the coding region (underlined) (bFcRnBACU_for: 59-ATC AGA ATT CCC TATAAATAT GCG GCT TCC CCG GCC T39); the reverse primer contains the gene-specific coding re- gions (underlined), a factor Xa site (italic), a 63His tag coding sequence, a stop codon and an EcoRI site (bFcRnBACU_rev: 59-ATC AGA ATT CCT AAT GAT GAT GAT GAT GAT GAC GAC CTT CGA TCA GCT CCA CCG TGA GGG GCT-39).

    Expressing:

    Article Title: FcRn mediates elongated serum half-life of human IgG in cattle.
    Article Snippet: .. MethodsExpression and purification of the soluble bovine FcRn The soluble bovine FcRn (sbFcRn) heavy-chain cDNA (116– 982 bp; GenBank AF139106) was PCR amplified with Deep Vent polymerase (New England Biolab, Beverly, MA, USA) from a eucaryotic expression construct described before (29). .. The forward primer contains an EcoRI site, a ‘Kozak’ sequence (italic) and the coding region (underlined) (bFcRnBACU_for: 59-ATC AGA ATT CCC TATAAATAT GCG GCT TCC CCG GCC T39); the reverse primer contains the gene-specific coding re- gions (underlined), a factor Xa site (italic), a 63His tag coding sequence, a stop codon and an EcoRI site (bFcRnBACU_rev: 59-ATC AGA ATT CCT AAT GAT GAT GAT GAT GAT GAC GAC CTT CGA TCA GCT CCA CCG TGA GGG GCT-39).

    Polymerase Chain Reaction:

    Article Title: Identification of a Human Papillomavirus Type 16-Specific Epitope on the C-Terminal Arm of the Major Capsid Protein L1
    Article Snippet: .. Deep Vent polymerase (New England Biolabs, Beverly, Mass.) was used for high fidelity PCR, PFU turbo (Stratagene La Jolla, Calif.) was used for mutagenesis, and Taq (Invitrogen) was for analysis of recombinant bacmids. .. The L1 amplimers were cloned into the Gateway pDonr201 vector and from there into the pDest8 vector with Gateway technology following the manufacturer's protocol (Invitrogen).

    Article Title: FcRn mediates elongated serum half-life of human IgG in cattle.
    Article Snippet: .. MethodsExpression and purification of the soluble bovine FcRn The soluble bovine FcRn (sbFcRn) heavy-chain cDNA (116– 982 bp; GenBank AF139106) was PCR amplified with Deep Vent polymerase (New England Biolab, Beverly, MA, USA) from a eucaryotic expression construct described before (29). .. The forward primer contains an EcoRI site, a ‘Kozak’ sequence (italic) and the coding region (underlined) (bFcRnBACU_for: 59-ATC AGA ATT CCC TATAAATAT GCG GCT TCC CCG GCC T39); the reverse primer contains the gene-specific coding re- gions (underlined), a factor Xa site (italic), a 63His tag coding sequence, a stop codon and an EcoRI site (bFcRnBACU_rev: 59-ATC AGA ATT CCT AAT GAT GAT GAT GAT GAT GAC GAC CTT CGA TCA GCT CCA CCG TGA GGG GCT-39).

    Article Title: Systematic evaluation of genome-wide methylated DNA enrichment using a CpG island array
    Article Snippet: .. PCR amplification of each enriched DNA fragment was performed in a 300 ul volume mixture comprised of the restriction DNA product, 10× ThermoPol Buffer, 2.5 uM H-24 primer, 0.2 mM dNTP mixture, and 12 U Deep Vent(exo-) DNA polymerase (New England Biolabs, USA). .. The amplification conditions were 5 min at 72°C to fill in the protruding ends of the ligated DNA fragments, followed by 20 cycles (1 min at 97°C and 3 min at 72°C), with a final extension for 10 min at 72°C.

    Article Title: Adenovirus Protein VII Condenses DNA, Represses Transcription, and Associates with Transcriptional Activator E1A
    Article Snippet: .. PCR was performed using Deep Vent polymerase (New England Biolabs) under standard conditions for 35 cycles of 1 min at 97°C, 1.5 min at 55°C, and 2 min at 72°C, using adenovirus- or rDNA-specific primers. ..

    Article Title: Selective Glutaraldehyde-Mediated Coupling of Proteins to the 3′-Adenine Terminus of Polymerase Chain Reaction Products
    Article Snippet: .. It is of interest to note that this band shift occurred after using Deep Vent (exo-) polymerase which, according to the manufacturer, is thought to produce 3′ adenine overhangs in only 30% of its PCR products. .. Even with this reduced abundance of 3′-adenine overhangs (versus nearly 100% 3′-adenine addition with Taq ), we were still able to detect a gel shift indicative of a DNA–protein conjugate in lane 7 of .

    Article Title: Glycoproteins M and N of Pseudorabies Virus Form a Disulfide-Linked Complex
    Article Snippet: .. The UL49.5 gene was amplified by PCR with Deep Vent polymerase (New England Biolabs, Schwalbach, Germany) and the following primers: gN-1, 5′-TGGATCCAGGATGGTCTCCTCCG-3′ (positions 365 to 377 of GenBank accession no. ), and gN-2, 5′-CGAATTCGATCTTTATACGCGCCGCC-3′ (positions 82 to 62). .. By using the Bam HI and Eco RI restriction sites contained in the primers, the PCR product was inserted into pVL1393 (PharMingen, San Diego, Calif.) under the control of the polyhedrin promoter, resulting in plasmid pVL1393gN.

    Recombinant:

    Article Title: Identification of a Human Papillomavirus Type 16-Specific Epitope on the C-Terminal Arm of the Major Capsid Protein L1
    Article Snippet: .. Deep Vent polymerase (New England Biolabs, Beverly, Mass.) was used for high fidelity PCR, PFU turbo (Stratagene La Jolla, Calif.) was used for mutagenesis, and Taq (Invitrogen) was for analysis of recombinant bacmids. .. The L1 amplimers were cloned into the Gateway pDonr201 vector and from there into the pDest8 vector with Gateway technology following the manufacturer's protocol (Invitrogen).

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  • About
  • News
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  • Team
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  • Contact
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  • 94
    New England Biolabs deep vent polymerase
    Deep Vent Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/deep vent polymerase/product/New England Biolabs
    Average 94 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    deep vent polymerase - by Bioz Stars, 2021-02
    94/100 stars
      Buy from Supplier

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