acaaccagctaaga  (New England Biolabs)


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    Name:
    M MuLV Reverse Transcriptase
    Description:
    M MuLV Reverse Transcriptase 50 000 units
    Catalog Number:
    m0253l
    Price:
    288
    Category:
    Reverse Transcriptases
    Size:
    50 000 units
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    New England Biolabs acaaccagctaaga
    M MuLV Reverse Transcriptase
    M MuLV Reverse Transcriptase 50 000 units
    https://www.bioz.com/result/acaaccagctaaga/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    acaaccagctaaga - by Bioz Stars, 2021-03
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The Imbalance between Foxp3+Tregs and Th1/Th17/Th22 Cells in Patients with Newly Diagnosed Autoimmune Hepatitis
    Article Snippet: .. Total RNA of 2 μ g was reverse transcribed with hexamer and M-MuLV reverse transcriptase (New England Biolabs, Ipswich, MA) according to the manufacturer's instructions. cDNA corresponding to 25 ng of total RNA was subjected to quantitative PCR (qPCR) using primer sets and TaqMan probes corresponding to murine Foxp3, T-bet, AHR, ROR γ t, and GAPDH with qPCR Master Mix. ..

    Article Title: High-efficiency generation of induced pluripotent mesenchymal stem cells from human dermal fibroblasts using recombinant proteins
    Article Snippet: Immunofluorescence assay, PCR, and western blotting of differentiated iPMSCs Induced osteogenic cells were fixed by 4 % paraformaldehyde (Sigma-Aldrich) and stained by anti-osteocalcin antibody (1:200 dilution; Santa Cruz); induced neurogenic cells were stained with anti-GFAP antibody (1:300 dilution; CST), anti-Nestin antibody (1:300 dilution; CST), anti-MAP2 antibody (1:200 dilution; Abcam), and anti-β-Tubulin III (1:200 dilution; Abcam); and induced pancreatic islet cells were stained with anti-Pdx1 antibody (1:400 dilution; CST), anti-glucagon antibody (1:400 dilution; CST), and anti-insulin antibody (1:400 dilution, 3014; CST) respectively. .. Total RNA was extracted using TRIZol® Reagent (Life Technologies) followed by cDNA synthesis using M-MuLV Reverse Transcriptase and Oligo (dT)23VN (NEB). qPCR was performed with iTaq™ Universal SYBR® Green Supermix (Bio Rad). .. Western blotting was performed for osteocalcin, microtubule-associated protein 2 (MAP2), β-Tubulin III, ADFP, and Plascidin L. All antibodies were validated for antigen specificity.

    Isolation:

    Article Title: Establishment and functional characterization of the reversibly immortalized mouse glomerular podocytes (imPODs)
    Article Snippet: .. Total RNA was isolated using TRIzol Reagents (Invitrogen, Carlsbad, CA, USA) as described., , The cDNA synthesis was carried out using hexamer and M-MuLV Reverse Transcriptase (New England Biolabs, Ipswich, MA, USA). .. The cDNA products were diluted 10- to 50-fold and used as PCR templates.

    Generated:

    Article Title: Spatiotemporal regulation of the GPCR activity of BAI3 by C1qL4 and Stabilin-2 controls myoblast fusion
    Article Snippet: Briefly, TRIZOL reagent (Invitrogen) was used according to the manufacturer protocol. .. Total RNAs were treated with DNAse1 (Invitrogen) and cDNAs were generated using the M-MuLV Reverse Transcriptase and random primers (NEB), as recommended by the manufacturer. .. Specific knockdown of the genes of interest was confirmed by real-time Q-PCR in an Mx3005P (Stratagene) system using SYBR Green PCR Master Mix (Applied Biosystems).

    Synthesized:

    Article Title: The Evolutionarily Conserved Cassette Exon 7b Drives ERG's Oncogenic Properties
    Article Snippet: Total RNA was extracted using the total RNA isolation mini kit (Agilent Technologies Ltd.). .. All samples were treated with DNAse on the columns using RNase-free DNase I provided in the kit. cDNA was synthesized from 0.2-1 μg of total RNA using 200 U MuLV reverse transcriptase (New England Biolabs), 40 U RNase inhibitor (human placenta) (New England Biolabs), 0.5 mM dNTP, 25 μM oligo-dT primers, and 10× reverse transcriptase buffer (500 mM Tris–HCl pH 8.3, 750 mM KCl, 30 mM MgCl2 , 100 mM DTT) (New England Biolabs) in a final reaction volume 20 μl with added nuclease-free water as required (Qiagen). .. Hot Start Taq 2× master mix (New England Biolabs) was used for standard PCR.

    SYBR Green Assay:

    Article Title: High-efficiency generation of induced pluripotent mesenchymal stem cells from human dermal fibroblasts using recombinant proteins
    Article Snippet: Immunofluorescence assay, PCR, and western blotting of differentiated iPMSCs Induced osteogenic cells were fixed by 4 % paraformaldehyde (Sigma-Aldrich) and stained by anti-osteocalcin antibody (1:200 dilution; Santa Cruz); induced neurogenic cells were stained with anti-GFAP antibody (1:300 dilution; CST), anti-Nestin antibody (1:300 dilution; CST), anti-MAP2 antibody (1:200 dilution; Abcam), and anti-β-Tubulin III (1:200 dilution; Abcam); and induced pancreatic islet cells were stained with anti-Pdx1 antibody (1:400 dilution; CST), anti-glucagon antibody (1:400 dilution; CST), and anti-insulin antibody (1:400 dilution, 3014; CST) respectively. .. Total RNA was extracted using TRIZol® Reagent (Life Technologies) followed by cDNA synthesis using M-MuLV Reverse Transcriptase and Oligo (dT)23VN (NEB). qPCR was performed with iTaq™ Universal SYBR® Green Supermix (Bio Rad). .. Western blotting was performed for osteocalcin, microtubule-associated protein 2 (MAP2), β-Tubulin III, ADFP, and Plascidin L. All antibodies were validated for antigen specificity.

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  • 99
    New England Biolabs m mulv
    Reverse transcription using AHP dUTP. ( A ) RNA template T5 with primer P3. ( B and C ) Twenty percent denaturing PAGE analysis of reactions using <t>AMV</t> and <t>M-MuLV</t> (RNase H-) reverse transcriptases at 42°C for 15 h.
    M Mulv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m mulv/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    m mulv - by Bioz Stars, 2021-03
    99/100 stars
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    Reverse transcription using AHP dUTP. ( A ) RNA template T5 with primer P3. ( B and C ) Twenty percent denaturing PAGE analysis of reactions using AMV and M-MuLV (RNase H-) reverse transcriptases at 42°C for 15 h.

    Journal: Nucleic Acids Research

    Article Title: Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes

    doi: 10.1093/nar/gkw028

    Figure Lengend Snippet: Reverse transcription using AHP dUTP. ( A ) RNA template T5 with primer P3. ( B and C ) Twenty percent denaturing PAGE analysis of reactions using AMV and M-MuLV (RNase H-) reverse transcriptases at 42°C for 15 h.

    Article Snippet: Klenow large fragment, Therminator™ II, M-MuLV (RNase H− ) reverse transcriptase, AMV reverse transcriptase, RNase inhibitor and λ-exonuclease were purchased from New England Biolabs.

    Techniques: Polyacrylamide Gel Electrophoresis

    Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C (M−MuLV reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart RTx Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p

    Journal: Journal of Advanced Research

    Article Title: A reverse transcriptase-mediated ribosomal RNA depletion (RTR2D) strategy for the cost-effective construction of RNA sequencing libraries

    doi: 10.1016/j.jare.2019.12.005

    Figure Lengend Snippet: Optimization of the reverse transcription conditions for the RTR2D protocol . ( A ) Effect of different reverse transcriptase and reaction temperatures on rRNA removal efficiency and specificity. Human total RNA (1.0 µg) was subjected to the RTR2D procedure under the same condition, except the use of different RT enzymes and reaction temperatures as follows: 37 °C (M−MuLV reverse transcriptase), 42 °C (ProtoScript® II Reverse Transcriptase), and 50 °C (WarmStart RTx Reverse Transcriptase). The Input and NP groups were used as controls. The expression levels of rRNAs were determined by TqPCR. “**” p

    Article Snippet: M−MuLV reverse transcriptase, ProtoScript® II Reverse Transcriptase, WarmStart RTx Reverse Transcriptase, RNase H, DNase I, Exonuclease I, Murine RNase Inhibitor, NEBNext® rRNA Depletion Kit (Human/Mouse/Rat), and NEBNext Ultra Directional RNA Library Prep Kit for Illumina were purchased from New England Biolabs (NEB, Ipswich, MA).

    Techniques: Expressing