haeiii methyltransferase  (New England Biolabs)


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  • 80
    Name:
    Hae III Methyltransferase
    Description:
    Hae III Methyltransferase 500 units
    Catalog Number:
    m0224s
    Price:
    74
    Size:
    500 units
    Category:
    DNA Methylases
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    Structured Review

    New England Biolabs haeiii methyltransferase
    Hae III Methyltransferase
    Hae III Methyltransferase 500 units
    https://www.bioz.com/result/haeiii methyltransferase/product/New England Biolabs
    Average 80 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    haeiii methyltransferase - by Bioz Stars, 2020-01
    80/100 stars

    Related Products / Commonly Used Together

    e. coli dh5α
    pdcw68 dna
    m.cbei

    Images

    1) Product Images from "The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41"

    Article Title: The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41

    Journal: BMC Microbiology

    doi: 10.1186/s12866-017-1014-6

    The transformation frequencies of pBU4 into L. sphaericus C3–41 and its derivate mutants. Light gray column: pBU4 untreated; deep gray column: pBU4 methylated with C3–41 CFE; black column: pBU4 methylated with MTase M. Hae III. *, no transformant observed
    Figure Legend Snippet: The transformation frequencies of pBU4 into L. sphaericus C3–41 and its derivate mutants. Light gray column: pBU4 untreated; deep gray column: pBU4 methylated with C3–41 CFE; black column: pBU4 methylated with MTase M. Hae III. *, no transformant observed

    Techniques Used: Transformation Assay, Methylation

    The effect of Bsph_0498 (encoding LspC3–41I) on the restriction role of L. sphaericus C3–41 CFE. Untreated and pre-treated plasmid pBU4 was incubated with CFE and then subjected to restriction assays, and the reaction mixture was analyzed by agarose gel electrophoresis as show in the left three pictures (L) and Southern blot analysis as show in the right three pictures (R). a untreated pBU4. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. b pBU4 methylated with C3–41 CFE. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. c pBU4 methylated with MTase M. Hae III. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. M: DNA marker
    Figure Legend Snippet: The effect of Bsph_0498 (encoding LspC3–41I) on the restriction role of L. sphaericus C3–41 CFE. Untreated and pre-treated plasmid pBU4 was incubated with CFE and then subjected to restriction assays, and the reaction mixture was analyzed by agarose gel electrophoresis as show in the left three pictures (L) and Southern blot analysis as show in the right three pictures (R). a untreated pBU4. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. b pBU4 methylated with C3–41 CFE. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. c pBU4 methylated with MTase M. Hae III. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. M: DNA marker

    Techniques Used: Plasmid Preparation, Incubation, Agarose Gel Electrophoresis, Southern Blot, Methylation, Marker

    Related Articles

    Centrifugation:

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: A cell-free extracts of C. bescii was prepared from a 500 ml culture grown to mid-log phase, harvested by centrifugation at 6,000 g at 4°C for 15 min and resuspended in Cel-Lytic B cell lysis reagent (Sigma) containing a protease inhibitor cocktail (Complete, EDTA-free from Roche). .. For in vitro methylation of DNA, pDCW68 DNA (20 µg), isolated from E. coli DH5α ( dam + , dcm + ), was treated with HaeIII methyltransferase (New England Biolabs) according to the supplier’s instructions.

    Amplification:

    Article Title: Activation of Caspases and p53 by Bovine Herpesvirus 1 Infection Results in Programmed Cell Death and Efficient Virus Release
    Article Snippet: .. The sizes of the amplified products were estimated by using φX174 replicative DNA cleaved with Hae III DNA (New England Biolabs). .. Uninfected or infected MDBK cells were rinsed twice with methionine-free Dulbecco modified Eagle medium (DMEM) (GIBCO-BRL) and incubated in methionine-free DMEM at 37°C for 30 min.

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: For in vitro methylation of DNA, pDCW68 DNA (20 µg), isolated from E. coli DH5α ( dam + , dcm + ), was treated with HaeIII methyltransferase (New England Biolabs) according to the supplier’s instructions. .. The 2.56-kb fragment containing three HaeIII (5′-GG CC-3′) sites used in assays with purified CbeI was generated by PCR amplification using primers DC222 and DC224 ( ) from pDCW68 template.

    Agarose Gel Electrophoresis:

    Article Title: The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41
    Article Snippet: The methylation with commercial MTase M. Hae III (New England Biolabs) was processed according to the recommended protocol by the manufacturer. .. All samples were incubated at 37 °C for 2 h. M. Hae III was heat inactivated at 65 °C for 20 min and removed by phenol-chloroform-isoamyl alcohol extraction as described previously [ ], then analyzed by agarose gel electrophoresis.

    In Vitro:

    Article Title: The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41
    Article Snippet: Paragraph title: In vitro methylation ... The methylation with commercial MTase M. Hae III (New England Biolabs) was processed according to the recommended protocol by the manufacturer.

    Article Title: Methylation by a Unique ?-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725
    Article Snippet: .. For in vitro methylation, DNA isolated from E. coli DH5α (dam+ dcm+ ) was treated with either M.CbeI or M.HaeIII methyltransferase (NEB). .. 5 microgram of purified M.CbeI was incubated with 50 mM Tris-HCl, 50 mM NaCl, 80 μM S-adenosylmethionine (SAM), 10 mM Dithiothreitol (DTT) at pH 8.5 and 20 µg of DNA substrate in 400 µl reaction, and incubate for 2 hours at 78°C.

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: .. For in vitro methylation of DNA, pDCW68 DNA (20 µg), isolated from E. coli DH5α ( dam + , dcm + ), was treated with HaeIII methyltransferase (New England Biolabs) according to the supplier’s instructions. .. To allow complete methylation, an additional 10 units of M.HaeIII and 80 µM S -adenosylmethionine (SAM) was added to the reaction every 4 h of incubation at 37°C for a total of 12 h. The methyltransferase was inactivated by incubation at 65°C for 15 min. Methylated DNA was purified and concentrated using the DNA Clean & Concentrator™-25 Kit (Zymo Research).

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: .. In addition, pDCW68 DNA that had been methylated in vitro by commercially available HaeIII methyltransferase was protected from cleavage by either the commercially available HaeIII restriction enzyme or cell-free extracts from C. bescii , implying the presence of cognate methyltransferase activity in C. bescii . .. In support of the notion that C. bescii contains a HaeIII-like enzyme is the fact that HaeIII recognition sites (GGCC) are much less abundant in the C. bescii genome sequence than would be expected based on random nucleotide composition (0.468 observed/expected).

    Methylation:

    Article Title: The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41
    Article Snippet: .. The methylation with commercial MTase M. Hae III (New England Biolabs) was processed according to the recommended protocol by the manufacturer. .. As a control, plasmid DNA was mock treated in a reaction mixture without CFE or M. Hae III.

    Article Title: Methylation by a Unique ?-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725
    Article Snippet: .. For in vitro methylation, DNA isolated from E. coli DH5α (dam+ dcm+ ) was treated with either M.CbeI or M.HaeIII methyltransferase (NEB). .. 5 microgram of purified M.CbeI was incubated with 50 mM Tris-HCl, 50 mM NaCl, 80 μM S-adenosylmethionine (SAM), 10 mM Dithiothreitol (DTT) at pH 8.5 and 20 µg of DNA substrate in 400 µl reaction, and incubate for 2 hours at 78°C.

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: .. For in vitro methylation of DNA, pDCW68 DNA (20 µg), isolated from E. coli DH5α ( dam + , dcm + ), was treated with HaeIII methyltransferase (New England Biolabs) according to the supplier’s instructions. .. To allow complete methylation, an additional 10 units of M.HaeIII and 80 µM S -adenosylmethionine (SAM) was added to the reaction every 4 h of incubation at 37°C for a total of 12 h. The methyltransferase was inactivated by incubation at 65°C for 15 min. Methylated DNA was purified and concentrated using the DNA Clean & Concentrator™-25 Kit (Zymo Research).

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: .. In addition, pDCW68 DNA that had been methylated in vitro by commercially available HaeIII methyltransferase was protected from cleavage by either the commercially available HaeIII restriction enzyme or cell-free extracts from C. bescii , implying the presence of cognate methyltransferase activity in C. bescii . .. In support of the notion that C. bescii contains a HaeIII-like enzyme is the fact that HaeIII recognition sites (GGCC) are much less abundant in the C. bescii genome sequence than would be expected based on random nucleotide composition (0.468 observed/expected).

    Ethanol Precipitation:

    Article Title: Methylation by a Unique ?-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725
    Article Snippet: For in vitro methylation, DNA isolated from E. coli DH5α (dam+ dcm+ ) was treated with either M.CbeI or M.HaeIII methyltransferase (NEB). .. To allow complete methylation, an additional 10 units of M.HaeIII and 80 µM SAM was added to the reaction every 4 h of incubation at 37°C for a total of 12 h. Methylated DNAs were purified and concentrated by Phenol/Chloroform extraction and ethanol precipitation.

    Protein Concentration:

    Article Title: Methylation by a Unique ?-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725
    Article Snippet: Protein concentration was determined by the Bio-Rad protein assay using bovine serum albumin (BSA) as the standard. .. For in vitro methylation, DNA isolated from E. coli DH5α (dam+ dcm+ ) was treated with either M.CbeI or M.HaeIII methyltransferase (NEB).

    Protease Inhibitor:

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: A cell-free extracts of C. bescii was prepared from a 500 ml culture grown to mid-log phase, harvested by centrifugation at 6,000 g at 4°C for 15 min and resuspended in Cel-Lytic B cell lysis reagent (Sigma) containing a protease inhibitor cocktail (Complete, EDTA-free from Roche). .. For in vitro methylation of DNA, pDCW68 DNA (20 µg), isolated from E. coli DH5α ( dam + , dcm + ), was treated with HaeIII methyltransferase (New England Biolabs) according to the supplier’s instructions.

    Infection:

    Article Title: Activation of Caspases and p53 by Bovine Herpesvirus 1 Infection Results in Programmed Cell Death and Efficient Virus Release
    Article Snippet: Total RNA from uninfected or infected cells was extracted by using RNAgents (total RNA isolation system from Promega; catalogue no. Z5110) according to the manufacturer’s instructions. .. The sizes of the amplified products were estimated by using φX174 replicative DNA cleaved with Hae III DNA (New England Biolabs).

    Purification:

    Article Title: Methylation by a Unique ?-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725
    Article Snippet: Paragraph title: Purification of His-tagged M.CbeI and in vitro methylation of DNA ... For in vitro methylation, DNA isolated from E. coli DH5α (dam+ dcm+ ) was treated with either M.CbeI or M.HaeIII methyltransferase (NEB).

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: For in vitro methylation of DNA, pDCW68 DNA (20 µg), isolated from E. coli DH5α ( dam + , dcm + ), was treated with HaeIII methyltransferase (New England Biolabs) according to the supplier’s instructions. .. To allow complete methylation, an additional 10 units of M.HaeIII and 80 µM S -adenosylmethionine (SAM) was added to the reaction every 4 h of incubation at 37°C for a total of 12 h. The methyltransferase was inactivated by incubation at 65°C for 15 min. Methylated DNA was purified and concentrated using the DNA Clean & Concentrator™-25 Kit (Zymo Research).

    Incubation:

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: .. Plasmid DNA from E. coli , but not plasmid DNA from C. bescii , was digested within 10 min of incubation with these extracts, and treatment with HaeIII methyltransferase protected the DNA from cleavage, suggesting the existence of a HaeIII-like restriction-modification system in C. bescii . ..

    Article Title: The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41
    Article Snippet: The methylation with commercial MTase M. Hae III (New England Biolabs) was processed according to the recommended protocol by the manufacturer. .. All samples were incubated at 37 °C for 2 h. M. Hae III was heat inactivated at 65 °C for 20 min and removed by phenol-chloroform-isoamyl alcohol extraction as described previously [ ], then analyzed by agarose gel electrophoresis.

    Article Title: Methylation by a Unique ?-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725
    Article Snippet: For in vitro methylation, DNA isolated from E. coli DH5α (dam+ dcm+ ) was treated with either M.CbeI or M.HaeIII methyltransferase (NEB). .. 5 microgram of purified M.CbeI was incubated with 50 mM Tris-HCl, 50 mM NaCl, 80 μM S-adenosylmethionine (SAM), 10 mM Dithiothreitol (DTT) at pH 8.5 and 20 µg of DNA substrate in 400 µl reaction, and incubate for 2 hours at 78°C.

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: For in vitro methylation of DNA, pDCW68 DNA (20 µg), isolated from E. coli DH5α ( dam + , dcm + ), was treated with HaeIII methyltransferase (New England Biolabs) according to the supplier’s instructions. .. To allow complete methylation, an additional 10 units of M.HaeIII and 80 µM S -adenosylmethionine (SAM) was added to the reaction every 4 h of incubation at 37°C for a total of 12 h. The methyltransferase was inactivated by incubation at 65°C for 15 min. Methylated DNA was purified and concentrated using the DNA Clean & Concentrator™-25 Kit (Zymo Research).

    Activity Assay:

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: .. In addition, pDCW68 DNA that had been methylated in vitro by commercially available HaeIII methyltransferase was protected from cleavage by either the commercially available HaeIII restriction enzyme or cell-free extracts from C. bescii , implying the presence of cognate methyltransferase activity in C. bescii . .. In support of the notion that C. bescii contains a HaeIII-like enzyme is the fact that HaeIII recognition sites (GGCC) are much less abundant in the C. bescii genome sequence than would be expected based on random nucleotide composition (0.468 observed/expected).

    Polyacrylamide Gel Electrophoresis:

    Article Title: Methylation by a Unique ?-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725
    Article Snippet: Purified protein was displayed using sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and stained with Coomassie brilliant blue G-250 as described . .. For in vitro methylation, DNA isolated from E. coli DH5α (dam+ dcm+ ) was treated with either M.CbeI or M.HaeIII methyltransferase (NEB).

    Isolation:

    Article Title: Activation of Caspases and p53 by Bovine Herpesvirus 1 Infection Results in Programmed Cell Death and Efficient Virus Release
    Article Snippet: Total RNA from uninfected or infected cells was extracted by using RNAgents (total RNA isolation system from Promega; catalogue no. Z5110) according to the manufacturer’s instructions. .. The sizes of the amplified products were estimated by using φX174 replicative DNA cleaved with Hae III DNA (New England Biolabs).

    Article Title: Methylation by a Unique ?-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725
    Article Snippet: .. For in vitro methylation, DNA isolated from E. coli DH5α (dam+ dcm+ ) was treated with either M.CbeI or M.HaeIII methyltransferase (NEB). .. 5 microgram of purified M.CbeI was incubated with 50 mM Tris-HCl, 50 mM NaCl, 80 μM S-adenosylmethionine (SAM), 10 mM Dithiothreitol (DTT) at pH 8.5 and 20 µg of DNA substrate in 400 µl reaction, and incubate for 2 hours at 78°C.

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: .. For in vitro methylation of DNA, pDCW68 DNA (20 µg), isolated from E. coli DH5α ( dam + , dcm + ), was treated with HaeIII methyltransferase (New England Biolabs) according to the supplier’s instructions. .. To allow complete methylation, an additional 10 units of M.HaeIII and 80 µM S -adenosylmethionine (SAM) was added to the reaction every 4 h of incubation at 37°C for a total of 12 h. The methyltransferase was inactivated by incubation at 65°C for 15 min. Methylated DNA was purified and concentrated using the DNA Clean & Concentrator™-25 Kit (Zymo Research).

    Polymerase Chain Reaction:

    Article Title: Activation of Caspases and p53 by Bovine Herpesvirus 1 Infection Results in Programmed Cell Death and Efficient Virus Release
    Article Snippet: Paragraph title: RNA PCR analysis. ... The sizes of the amplified products were estimated by using φX174 replicative DNA cleaved with Hae III DNA (New England Biolabs).

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: For in vitro methylation of DNA, pDCW68 DNA (20 µg), isolated from E. coli DH5α ( dam + , dcm + ), was treated with HaeIII methyltransferase (New England Biolabs) according to the supplier’s instructions. .. The 2.56-kb fragment containing three HaeIII (5′-GG CC-3′) sites used in assays with purified CbeI was generated by PCR amplification using primers DC222 and DC224 ( ) from pDCW68 template.

    Staining:

    Article Title: Methylation by a Unique ?-class N4-Cytosine Methyltransferase Is Required for DNA Transformation of Caldicellulosiruptor bescii DSM6725
    Article Snippet: Purified protein was displayed using sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and stained with Coomassie brilliant blue G-250 as described . .. For in vitro methylation, DNA isolated from E. coli DH5α (dam+ dcm+ ) was treated with either M.CbeI or M.HaeIII methyltransferase (NEB).

    Lysis:

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: A cell-free extracts of C. bescii was prepared from a 500 ml culture grown to mid-log phase, harvested by centrifugation at 6,000 g at 4°C for 15 min and resuspended in Cel-Lytic B cell lysis reagent (Sigma) containing a protease inhibitor cocktail (Complete, EDTA-free from Roche). .. For in vitro methylation of DNA, pDCW68 DNA (20 µg), isolated from E. coli DH5α ( dam + , dcm + ), was treated with HaeIII methyltransferase (New England Biolabs) according to the supplier’s instructions.

    Plasmid Preparation:

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: .. Plasmid DNA from E. coli , but not plasmid DNA from C. bescii , was digested within 10 min of incubation with these extracts, and treatment with HaeIII methyltransferase protected the DNA from cleavage, suggesting the existence of a HaeIII-like restriction-modification system in C. bescii . ..

    Article Title: The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41
    Article Snippet: 5 μg plasmid DNA was mixed with 20 μL CFE in a reaction buffer with TNM buffer (50 μL TNM buffer was added into a 100 μL in vitro methylation reaction), 30 mM EDTA (pH 8.0) and 200 μM SAM (S-adenosylmethionine), of which the EDTA was used to inhibit the restriction of CFE, and SAM to provide methyl. .. The methylation with commercial MTase M. Hae III (New England Biolabs) was processed according to the recommended protocol by the manufacturer.

    Generated:

    Article Title: Identification and characterization of CbeI, a novel thermostable restriction enzyme from Caldicellulosiruptor bescii DSM 6725 and a member of a new subfamily of HaeIII-like enzymes
    Article Snippet: For in vitro methylation of DNA, pDCW68 DNA (20 µg), isolated from E. coli DH5α ( dam + , dcm + ), was treated with HaeIII methyltransferase (New England Biolabs) according to the supplier’s instructions. .. The 2.56-kb fragment containing three HaeIII (5′-GG CC-3′) sites used in assays with purified CbeI was generated by PCR amplification using primers DC222 and DC224 ( ) from pDCW68 template.

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  • 80
    New England Biolabs haeiii methyltransferase
    The transformation frequencies of pBU4 into L. sphaericus C3–41 and its derivate mutants. Light gray column: pBU4 untreated; deep gray column: pBU4 methylated with C3–41 CFE; black column: pBU4 methylated with MTase M. Hae <t>III.</t> *, no transformant observed
    Haeiii Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/haeiii methyltransferase/product/New England Biolabs
    Average 80 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    haeiii methyltransferase - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

    Image Search Results


    The transformation frequencies of pBU4 into L. sphaericus C3–41 and its derivate mutants. Light gray column: pBU4 untreated; deep gray column: pBU4 methylated with C3–41 CFE; black column: pBU4 methylated with MTase M. Hae III. *, no transformant observed

    Journal: BMC Microbiology

    Article Title: The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41

    doi: 10.1186/s12866-017-1014-6

    Figure Lengend Snippet: The transformation frequencies of pBU4 into L. sphaericus C3–41 and its derivate mutants. Light gray column: pBU4 untreated; deep gray column: pBU4 methylated with C3–41 CFE; black column: pBU4 methylated with MTase M. Hae III. *, no transformant observed

    Article Snippet: The methylation with commercial MTase M. Hae III (New England Biolabs) was processed according to the recommended protocol by the manufacturer.

    Techniques: Transformation Assay, Methylation

    The effect of Bsph_0498 (encoding LspC3–41I) on the restriction role of L. sphaericus C3–41 CFE. Untreated and pre-treated plasmid pBU4 was incubated with CFE and then subjected to restriction assays, and the reaction mixture was analyzed by agarose gel electrophoresis as show in the left three pictures (L) and Southern blot analysis as show in the right three pictures (R). a untreated pBU4. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. b pBU4 methylated with C3–41 CFE. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. c pBU4 methylated with MTase M. Hae III. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. M: DNA marker

    Journal: BMC Microbiology

    Article Title: The LspC3–41I restriction-modification system is the major determinant for genetic manipulations of Lysinibacillus sphaericus C3–41

    doi: 10.1186/s12866-017-1014-6

    Figure Lengend Snippet: The effect of Bsph_0498 (encoding LspC3–41I) on the restriction role of L. sphaericus C3–41 CFE. Untreated and pre-treated plasmid pBU4 was incubated with CFE and then subjected to restriction assays, and the reaction mixture was analyzed by agarose gel electrophoresis as show in the left three pictures (L) and Southern blot analysis as show in the right three pictures (R). a untreated pBU4. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. b pBU4 methylated with C3–41 CFE. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. c pBU4 methylated with MTase M. Hae III. Lane 1, C3–41; lane 2, Δ0498; lane 3, RC0498; lane 4, Hae III digested; lane 5, Untreated. M: DNA marker

    Article Snippet: The methylation with commercial MTase M. Hae III (New England Biolabs) was processed according to the recommended protocol by the manufacturer.

    Techniques: Plasmid Preparation, Incubation, Agarose Gel Electrophoresis, Southern Blot, Methylation, Marker