set7 9  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 96
    Name:
    BamH I Methyltransferase
    Description:
    BamH I Methyltransferase 500 units
    Catalog Number:
    m0223l
    Price:
    315
    Size:
    500 units
    Category:
    DNA Methylases
    		Buy from Supplier

    Structured Review

    New England Biolabs set7 9
    BamH I Methyltransferase
    BamH I Methyltransferase 500 units
    https://www.bioz.com/result/set7 9/product/New England Biolabs
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    set7 9 - by Bioz Stars, 2020-03
    96/100 stars

    Related Products / Commonly Used Together

    e2f1
    pdx1
    tris-buffered saline
    cyclin e1
    secondary antibodies
    cdk2
    cyclin a2
    z1 inverted microscope
    anti-gapdh
    p53
    p21
    histone3.1
    anti-actin
    p65
    methylation buffer і

    Images

    1) Product Images from "A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity"

    Article Title: A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity

    Journal: Science Advances

    doi: 10.1126/sciadv.aav2623

    K-OPL reveals the substrate selectivity of G9a, SET7/9, and SMYD2. K-OPL substrate selectivity profiles for G9a ( A ), SET7/9 ( B ), and SMYD2 ( C ). Mean results of two independent K-OPL SPA screens for each enzyme are reported as position-normalized heat maps (see fig. S1 for global normalized heat maps and raw K-OPL data). The color code is proportional to the creation of enzyme product, where red (1) is most active and blue (0) is least active. Rows show the identity of each fixed residue, and columns show the position within the sequence. Initial rate measurements with peptides corresponding to known and newly identified substrates for G9a ( D ), SET7/9 ( E ), or SMYD2 ( F ). cpm, counts per minute. Point mutations predicted to decrease or increase the rate of methylation are indicated in red or green, respectively. Data points are shown as the mean of three independent measurements, and error is presented as ±SEM. For some data points, error bars are masked by the symbol weight.
    Figure Legend Snippet: K-OPL reveals the substrate selectivity of G9a, SET7/9, and SMYD2. K-OPL substrate selectivity profiles for G9a ( A ), SET7/9 ( B ), and SMYD2 ( C ). Mean results of two independent K-OPL SPA screens for each enzyme are reported as position-normalized heat maps (see fig. S1 for global normalized heat maps and raw K-OPL data). The color code is proportional to the creation of enzyme product, where red (1) is most active and blue (0) is least active. Rows show the identity of each fixed residue, and columns show the position within the sequence. Initial rate measurements with peptides corresponding to known and newly identified substrates for G9a ( D ), SET7/9 ( E ), or SMYD2 ( F ). cpm, counts per minute. Point mutations predicted to decrease or increase the rate of methylation are indicated in red or green, respectively. Data points are shown as the mean of three independent measurements, and error is presented as ±SEM. For some data points, error bars are masked by the symbol weight.

    Techniques Used: Sequencing, Methylation

    MS analysis of methylation products. The products from reactions of G9a ( A ), SET7/9 ( B ), and SMYD2 ( C ) with their corresponding peptide substrates were analyzed by MS. Mass spectra are shown in the absence (top) or presence (bottom) of enzyme treatment, as indicated.
    Figure Legend Snippet: MS analysis of methylation products. The products from reactions of G9a ( A ), SET7/9 ( B ), and SMYD2 ( C ) with their corresponding peptide substrates were analyzed by MS. Mass spectra are shown in the absence (top) or presence (bottom) of enzyme treatment, as indicated.

    Techniques Used: Mass Spectrometry, Methylation

    2) Product Images from "A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity"

    Article Title: A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity

    Journal: Science Advances

    doi: 10.1126/sciadv.aav2623

    K-OPL reveals the substrate selectivity of G9a, SET7/9, and SMYD2. K-OPL substrate selectivity profiles for G9a ( A ), SET7/9 ( B ), and SMYD2 ( C ). Mean results of two independent K-OPL SPA screens for each enzyme are reported as position-normalized heat maps (see fig. S1 for global normalized heat maps and raw K-OPL data). The color code is proportional to the creation of enzyme product, where red (1) is most active and blue (0) is least active. Rows show the identity of each fixed residue, and columns show the position within the sequence. Initial rate measurements with peptides corresponding to known and newly identified substrates for G9a ( D ), SET7/9 ( E ), or SMYD2 ( F ). cpm, counts per minute. Point mutations predicted to decrease or increase the rate of methylation are indicated in red or green, respectively. Data points are shown as the mean of three independent measurements, and error is presented as ±SEM. For some data points, error bars are masked by the symbol weight.
    Figure Legend Snippet: K-OPL reveals the substrate selectivity of G9a, SET7/9, and SMYD2. K-OPL substrate selectivity profiles for G9a ( A ), SET7/9 ( B ), and SMYD2 ( C ). Mean results of two independent K-OPL SPA screens for each enzyme are reported as position-normalized heat maps (see fig. S1 for global normalized heat maps and raw K-OPL data). The color code is proportional to the creation of enzyme product, where red (1) is most active and blue (0) is least active. Rows show the identity of each fixed residue, and columns show the position within the sequence. Initial rate measurements with peptides corresponding to known and newly identified substrates for G9a ( D ), SET7/9 ( E ), or SMYD2 ( F ). cpm, counts per minute. Point mutations predicted to decrease or increase the rate of methylation are indicated in red or green, respectively. Data points are shown as the mean of three independent measurements, and error is presented as ±SEM. For some data points, error bars are masked by the symbol weight.

    Techniques Used: Sequencing, Methylation

    MS analysis of methylation products. The products from reactions of G9a ( A ), SET7/9 ( B ), and SMYD2 ( C ) with their corresponding peptide substrates were analyzed by MS. Mass spectra are shown in the absence (top) or presence (bottom) of enzyme treatment, as indicated.
    Figure Legend Snippet: MS analysis of methylation products. The products from reactions of G9a ( A ), SET7/9 ( B ), and SMYD2 ( C ) with their corresponding peptide substrates were analyzed by MS. Mass spectra are shown in the absence (top) or presence (bottom) of enzyme treatment, as indicated.

    Techniques Used: Mass Spectrometry, Methylation

    Related Articles

    Produced:

    Article Title: A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity
    Article Snippet: SET7/9 was purchased from New England BioLabs (catalog no. M0223). .. G9a (amino acids 913 to 1210) was produced as a 6XHis N-terminal fusion, and SMYD2 (full length) was expressed as a N-terminal glutathione S -transferase (GST) fusion.

    Recombinant:

    Article Title: A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity
    Article Snippet: Paragraph title: Recombinant protein production ... SET7/9 was purchased from New England BioLabs (catalog no. M0223).

    Plasmid Preparation:

    Article Title: A functional proteomics platform to reveal the sequence determinants of lysine methyltransferase substrate selectivity
    Article Snippet: SET7/9 was purchased from New England BioLabs (catalog no. M0223). .. PRDM11 (amino acids 79 to 314) (Addgene plasmid no. 32858) and full-length human TP53 (Addgene plasmid no. 24859) were gifts from C. Arrowsmith.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars

    • Image Search Results