Journal: BioMed Research International

Article Title: Osteoponin Promoter Controlled by DNA Methylation: Aberrant Methylation in Cloned Porcine Genome

doi: 10.1155/2014/327538

Figure Lengend Snippet: Methylation and deletion analysis of OPN promoter in 293T cells. The match-like bar with black circle represents the methylation CpG site; white circle of match bar indicated the unmethylation CpG sites. PGL3 vector as standard; in 293T cell line; PGL3-enhance vector as negative control; cell lysate as the background; pCMV-b-gal as internal control. The relative value is adjusted by cell lysate; −495 M-luc indicated the methylation in PGL3 backbone with Hha I and Hpa II methyltransferase. (** P < 0.01); −2615-luc and −2615 M-luc: n = 3; −495-luc and −495 M-luc: n = 4. The experiments were repeated three times and the results were analyzed and presented as the mean ± SE.

Article Snippet: The −2615-luc construct was methylated by Hha I and Hpa II methyltransferase (New England BioLabs) for 16 h at 37°C.

Techniques: Methylation, Plasmid Preparation, Negative Control

Journal: BioMed Research International

Article Title: Osteoponin Promoter Controlled by DNA Methylation: Aberrant Methylation in Cloned Porcine Genome

doi: 10.1155/2014/327538

Figure Lengend Snippet: The sequence and CpG sties distribution of pig OPN promoter. 20 CpG sites exist in the front region of OPN promoter. The underline indicates the predicted AP1-like binding site. The gray marker indicates two methyltransferase sites of Hha I (GCGC) and Hpa II (CCGG).

Article Snippet: The −2615-luc construct was methylated by Hha I and Hpa II methyltransferase (New England BioLabs) for 16 h at 37°C.

Techniques: Sequencing, Binding Assay, Marker