hpaii mtase  (New England Biolabs)


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    New England Biolabs hpaii mtase
    Hpaii Mtase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    hpaii mtase  (New England Biolabs)


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    New England Biolabs hpaii mtase
    Hpaii Mtase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    hpa ii methyltransferase  (New England Biolabs)


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    New England Biolabs hpa ii methyltransferase
    Methylation and deletion analysis of OPN promoter in 293T cells. The match-like bar with black circle represents the methylation CpG site; white circle of match bar indicated the unmethylation CpG sites. PGL3 vector as standard; in 293T cell line; PGL3-enhance vector as negative control; cell lysate as the background; pCMV-b-gal as internal control. The relative value is adjusted by cell lysate; −495 M-luc indicated the methylation in PGL3 backbone with Hha I and <t>Hpa</t> II <t>methyltransferase.</t> (** P < 0.01); −2615-luc and −2615 M-luc: n = 3; −495-luc and −495 M-luc: n = 4. The experiments were repeated three times and the results were analyzed and presented as the mean ± SE.
    Hpa Ii Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpa ii methyltransferase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpa ii methyltransferase - by Bioz Stars, 2023-01
    94/100 stars

    Images

    1) Product Images from "Osteoponin Promoter Controlled by DNA Methylation: Aberrant Methylation in Cloned Porcine Genome"

    Article Title: Osteoponin Promoter Controlled by DNA Methylation: Aberrant Methylation in Cloned Porcine Genome

    Journal: BioMed Research International

    doi: 10.1155/2014/327538

    Methylation and deletion analysis of OPN promoter in 293T cells. The match-like bar with black circle represents the methylation CpG site; white circle of match bar indicated the unmethylation CpG sites. PGL3 vector as standard; in 293T cell line; PGL3-enhance vector as negative control; cell lysate as the background; pCMV-b-gal as internal control. The relative value is adjusted by cell lysate; −495 M-luc indicated the methylation in PGL3 backbone with Hha I and Hpa II methyltransferase. (** P < 0.01); −2615-luc and −2615 M-luc: n = 3; −495-luc and −495 M-luc: n = 4. The experiments were repeated three times and the results were analyzed and presented as the mean ± SE.
    Figure Legend Snippet: Methylation and deletion analysis of OPN promoter in 293T cells. The match-like bar with black circle represents the methylation CpG site; white circle of match bar indicated the unmethylation CpG sites. PGL3 vector as standard; in 293T cell line; PGL3-enhance vector as negative control; cell lysate as the background; pCMV-b-gal as internal control. The relative value is adjusted by cell lysate; −495 M-luc indicated the methylation in PGL3 backbone with Hha I and Hpa II methyltransferase. (** P < 0.01); −2615-luc and −2615 M-luc: n = 3; −495-luc and −495 M-luc: n = 4. The experiments were repeated three times and the results were analyzed and presented as the mean ± SE.

    Techniques Used: Methylation, Plasmid Preparation, Negative Control

    The sequence and CpG sties distribution of pig OPN promoter. 20 CpG sites exist in the front region of OPN promoter. The underline indicates the predicted AP1-like binding site. The gray marker indicates two methyltransferase sites of Hha I (GCGC) and Hpa II (CCGG).
    Figure Legend Snippet: The sequence and CpG sties distribution of pig OPN promoter. 20 CpG sites exist in the front region of OPN promoter. The underline indicates the predicted AP1-like binding site. The gray marker indicates two methyltransferase sites of Hha I (GCGC) and Hpa II (CCGG).

    Techniques Used: Sequencing, Binding Assay, Marker

    hpaii methyltransferase  (New England Biolabs)


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    New England Biolabs hpaii methyltransferase
    Hpaii Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    hpaii methyltransferase  (New England Biolabs)


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    New England Biolabs hpaii methyltransferase
    Hpaii Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    hpaii methyltransferases  (New England Biolabs)


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    New England Biolabs hpaii methyltransferases
    Hpaii Methyltransferases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hpaii methyltransferases  (New England Biolabs)


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    New England Biolabs hpaii methyltransferases
    Hpaii Methyltransferases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hpaii methyltransferase  (New England Biolabs)


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    New England Biolabs hpaii methyltransferase
    Hpaii Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpaii methyltransferase/product/New England Biolabs
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    hpaii methyltransferase  (New England Biolabs)


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    New England Biolabs hpaii methyltransferase
    Hpaii Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    hpaii methyltransferase  (New England Biolabs)


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    New England Biolabs hpaii methyltransferase
    Hpaii Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpaii methyltransferase/product/New England Biolabs
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    hpaii methyltransferase  (New England Biolabs)


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    New England Biolabs hpaii methyltransferase
    (A) Architecture of Dnmt5. CD: chromodomain; DNMT: DNA <t>methyltransferase</t> domain; SNF2-like: Swi/Snf ATPase domain.
    Hpaii Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Evolutionary persistence of DNA methylation for millions of years after ancient loss of a de novo methyltransferase"

    Article Title: Evolutionary persistence of DNA methylation for millions of years after ancient loss of a de novo methyltransferase

    Journal: Cell

    doi: 10.1016/j.cell.2019.12.012

    (A) Architecture of Dnmt5. CD: chromodomain; DNMT: DNA methyltransferase domain; SNF2-like: Swi/Snf ATPase domain.
    Figure Legend Snippet: (A) Architecture of Dnmt5. CD: chromodomain; DNMT: DNA methyltransferase domain; SNF2-like: Swi/Snf ATPase domain.

    Techniques Used:

    (A) A DNA fragment was methylated in vitro using the HpaII DNA methyltransferase (HpaII MT) and subsequently integrated into C. neoformans. gDNA extracted from isolates that correctly integrated the fragment, was digested with the restriction enzyme HpaII. To test the maintenance of the in vitro-methylated 5mC, qPCR was performed on region spanning the restriction sites. To test the ability 5mC to spread, gDNA was digested with either Hpy99 or BstUI and qPCR was performed across these sites. White and black lollipops represent unmethylated and methylated sites, respectively.
    Figure Legend Snippet: (A) A DNA fragment was methylated in vitro using the HpaII DNA methyltransferase (HpaII MT) and subsequently integrated into C. neoformans. gDNA extracted from isolates that correctly integrated the fragment, was digested with the restriction enzyme HpaII. To test the maintenance of the in vitro-methylated 5mC, qPCR was performed on region spanning the restriction sites. To test the ability 5mC to spread, gDNA was digested with either Hpy99 or BstUI and qPCR was performed across these sites. White and black lollipops represent unmethylated and methylated sites, respectively.

    Techniques Used: Methylation, In Vitro

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    New England Biolabs hpaii mtase
    Hpaii Mtase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpaii mtase/product/New England Biolabs
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    New England Biolabs hpa ii methyltransferase
    Methylation and deletion analysis of OPN promoter in 293T cells. The match-like bar with black circle represents the methylation CpG site; white circle of match bar indicated the unmethylation CpG sites. PGL3 vector as standard; in 293T cell line; PGL3-enhance vector as negative control; cell lysate as the background; pCMV-b-gal as internal control. The relative value is adjusted by cell lysate; −495 M-luc indicated the methylation in PGL3 backbone with Hha I and <t>Hpa</t> II <t>methyltransferase.</t> (** P < 0.01); −2615-luc and −2615 M-luc: n = 3; −495-luc and −495 M-luc: n = 4. The experiments were repeated three times and the results were analyzed and presented as the mean ± SE.
    Hpa Ii Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpa ii methyltransferase/product/New England Biolabs
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    hpa ii methyltransferase - by Bioz Stars, 2023-01
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    New England Biolabs hpaii methyltransferase
    Methylation and deletion analysis of OPN promoter in 293T cells. The match-like bar with black circle represents the methylation CpG site; white circle of match bar indicated the unmethylation CpG sites. PGL3 vector as standard; in 293T cell line; PGL3-enhance vector as negative control; cell lysate as the background; pCMV-b-gal as internal control. The relative value is adjusted by cell lysate; −495 M-luc indicated the methylation in PGL3 backbone with Hha I and <t>Hpa</t> II <t>methyltransferase.</t> (** P < 0.01); −2615-luc and −2615 M-luc: n = 3; −495-luc and −495 M-luc: n = 4. The experiments were repeated three times and the results were analyzed and presented as the mean ± SE.
    Hpaii Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpaii methyltransferase/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpaii methyltransferase - by Bioz Stars, 2023-01
    94/100 stars
      Buy from Supplier

    94
    New England Biolabs hpaii methyltransferases
    Methylation and deletion analysis of OPN promoter in 293T cells. The match-like bar with black circle represents the methylation CpG site; white circle of match bar indicated the unmethylation CpG sites. PGL3 vector as standard; in 293T cell line; PGL3-enhance vector as negative control; cell lysate as the background; pCMV-b-gal as internal control. The relative value is adjusted by cell lysate; −495 M-luc indicated the methylation in PGL3 backbone with Hha I and <t>Hpa</t> II <t>methyltransferase.</t> (** P < 0.01); −2615-luc and −2615 M-luc: n = 3; −495-luc and −495 M-luc: n = 4. The experiments were repeated three times and the results were analyzed and presented as the mean ± SE.
    Hpaii Methyltransferases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hpaii methyltransferases/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpaii methyltransferases - by Bioz Stars, 2023-01
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    Methylation and deletion analysis of OPN promoter in 293T cells. The match-like bar with black circle represents the methylation CpG site; white circle of match bar indicated the unmethylation CpG sites. PGL3 vector as standard; in 293T cell line; PGL3-enhance vector as negative control; cell lysate as the background; pCMV-b-gal as internal control. The relative value is adjusted by cell lysate; −495 M-luc indicated the methylation in PGL3 backbone with Hha I and Hpa II methyltransferase. (** P < 0.01); −2615-luc and −2615 M-luc: n = 3; −495-luc and −495 M-luc: n = 4. The experiments were repeated three times and the results were analyzed and presented as the mean ± SE.

    Journal: BioMed Research International

    Article Title: Osteoponin Promoter Controlled by DNA Methylation: Aberrant Methylation in Cloned Porcine Genome

    doi: 10.1155/2014/327538

    Figure Lengend Snippet: Methylation and deletion analysis of OPN promoter in 293T cells. The match-like bar with black circle represents the methylation CpG site; white circle of match bar indicated the unmethylation CpG sites. PGL3 vector as standard; in 293T cell line; PGL3-enhance vector as negative control; cell lysate as the background; pCMV-b-gal as internal control. The relative value is adjusted by cell lysate; −495 M-luc indicated the methylation in PGL3 backbone with Hha I and Hpa II methyltransferase. (** P < 0.01); −2615-luc and −2615 M-luc: n = 3; −495-luc and −495 M-luc: n = 4. The experiments were repeated three times and the results were analyzed and presented as the mean ± SE.

    Article Snippet: The −2615-luc construct was methylated by Hha I and Hpa II methyltransferase (New England BioLabs) for 16 h at 37°C.

    Techniques: Methylation, Plasmid Preparation, Negative Control

    The sequence and CpG sties distribution of pig OPN promoter. 20 CpG sites exist in the front region of OPN promoter. The underline indicates the predicted AP1-like binding site. The gray marker indicates two methyltransferase sites of Hha I (GCGC) and Hpa II (CCGG).

    Journal: BioMed Research International

    Article Title: Osteoponin Promoter Controlled by DNA Methylation: Aberrant Methylation in Cloned Porcine Genome

    doi: 10.1155/2014/327538

    Figure Lengend Snippet: The sequence and CpG sties distribution of pig OPN promoter. 20 CpG sites exist in the front region of OPN promoter. The underline indicates the predicted AP1-like binding site. The gray marker indicates two methyltransferase sites of Hha I (GCGC) and Hpa II (CCGG).

    Article Snippet: The −2615-luc construct was methylated by Hha I and Hpa II methyltransferase (New England BioLabs) for 16 h at 37°C.

    Techniques: Sequencing, Binding Assay, Marker