taq  (New England Biolabs)


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  • 99
    Name:
    Taq DNA Ligase
    Description:
    Taq DNA Ligase 10 000 units
    Catalog Number:
    M0208L
    Price:
    320
    Category:
    DNA Ligases
    Size:
    10 000 units
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    Structured Review

    New England Biolabs taq
    Taq DNA Ligase
    Taq DNA Ligase 10 000 units
    https://www.bioz.com/result/taq/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taq - by Bioz Stars, 2021-06
    99/100 stars

    Images

    1) Product Images from "A low-cost open-source SNP genotyping platform for association mapping applications"

    Article Title: A low-cost open-source SNP genotyping platform for association mapping applications

    Journal: Genome Biology

    doi: 10.1186/gb-2005-6-12-r105

    Principle of OLA-based SNP genotyping. (a) For each polymorphism, a set of three genotyping oligos are allowed to anneal to denatured PCR product (blue) in the presence of Taq DNA ligase. Ligation of up- and downstream oligos occurs only if there is a perfect match to template. Upstream oligos are color-coded gray (M13 forward amplification primer sequence), red/green (a pair of barcode sequences), and black (assay-specific sequence flanking the query SNP). The downstream oligo is 5'-phosphorylated, and color-coded gray (reverse complemented sequence of the M13 reverse amplification primer), and black (assay-specific flanking sequence). (b) Addition of common M13 primers (gray) allows amplification of all ligated products. (c) After arraying amplified OLA products, membranes are hybridized with probes complementary to the barcode sequences. Probes can be fluorescently labeled with infrared (IR) fluors and both alleles hybridized simultaneously, or radiolabeled and hybridized sequentially.
    Figure Legend Snippet: Principle of OLA-based SNP genotyping. (a) For each polymorphism, a set of three genotyping oligos are allowed to anneal to denatured PCR product (blue) in the presence of Taq DNA ligase. Ligation of up- and downstream oligos occurs only if there is a perfect match to template. Upstream oligos are color-coded gray (M13 forward amplification primer sequence), red/green (a pair of barcode sequences), and black (assay-specific sequence flanking the query SNP). The downstream oligo is 5'-phosphorylated, and color-coded gray (reverse complemented sequence of the M13 reverse amplification primer), and black (assay-specific flanking sequence). (b) Addition of common M13 primers (gray) allows amplification of all ligated products. (c) After arraying amplified OLA products, membranes are hybridized with probes complementary to the barcode sequences. Probes can be fluorescently labeled with infrared (IR) fluors and both alleles hybridized simultaneously, or radiolabeled and hybridized sequentially.

    Techniques Used: Polymerase Chain Reaction, Ligation, Amplification, Sequencing, Labeling

    Related Articles

    Incubation:

    Article Title: Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus
    Article Snippet: Purified NC-DNA from Hep38.7-Tet cells was used as substrate DNA. .. NC-DNA (108 copies) was incubated with 32 units (U) of Thermostable FEN1 in ThermoPol Buffer (New England Biolabs) at 65°C for 10 min, followed by incubation with 8 U of Bst DNA polymerase, 40 U of Taq DNA ligase, 100 μM dNTPs, and NAD+ (all from New England Biolabs). .. After further incubation at 37°C for 20 min, DNA was purified by phenol/chloroform extraction and ethanol precipitation, and subjected to cccDNA-selective qPCR or RCA, as described above.

    Clone Assay:

    Article Title: T5 exonuclease-dependent assembly offers a low-cost method for efficient cloning and site-directed mutagenesis
    Article Snippet: The PCR and sequencing primers for the plasmids used in TEDA optimization and testing were listed in . .. T5 exonuclease in a Tris buffer with PEG 8000 works well for cloning The Gibson method applies three enzymes, and it can be simplified by removing Taq DNA ligase without reducing the cloning efficiency ( , ). .. To test whether the method could be further simplified, we checked the requirement of the enzymes and other components in the Gibson system for cloning Pkat-eGFP into pBluescript SK- (Figure ).

    Ligation:

    Article Title: SNPWaveTM: a flexible multiplexed SNP genotyping technology
    Article Snippet: .. Ligation reactions for Arabidopsis polymorphisms were performed in a 25 µl volume containing 625 ng of Arabidopsis DNA, 1× Taq DNA ligase buffer [20 mM Tris–HCl, 25 mM KAc, 10 mM MgAc2 , 10 mM dithiothreitol (DTT), 1 mM NAD, 0.1% Triton X-100; pH 7.6 at 25°C; New England Biolabs Inc., Beverly, MA], 0.2 U/µl Taq DNA ligase (NEB) and 0.05 fmol/µl of each of 200 ligation probes. .. Next, 10 cycles of repeated denaturation, probe hybridization and ligation were performed in a Perkin Elmer 9700 thermal cycler (Applied Biosystems, Foster City, CA) using the following profile: initial denaturation for 2 min at 94°C, followed by 10 cycles of 15 s at 94°C and 60 min at 60°C, followed by storage at 4°C.

    Article Title: A Comparative Study on the Expression, Purification and Functional Characterization of Human Adiponectin in Pichia pastoris and Escherichia coli
    Article Snippet: .. The ligation mixture was prepared by adding digested vector and digested ADP fragment with DNA ligase and its suitable ligation buffer (New England Biolabs, UK). .. Adiponectin fragment was cloned in pGEM® -T cloning vector (Promega, USA).

    Plasmid Preparation:

    Article Title: A Comparative Study on the Expression, Purification and Functional Characterization of Human Adiponectin in Pichia pastoris and Escherichia coli
    Article Snippet: .. The ligation mixture was prepared by adding digested vector and digested ADP fragment with DNA ligase and its suitable ligation buffer (New England Biolabs, UK). .. Adiponectin fragment was cloned in pGEM® -T cloning vector (Promega, USA).

    Article Title: Seamless Insert-Plasmid Assembly at High Efficiency and Low Cost
    Article Snippet: .. Each Gibson assembly reaction consisted of 2.7 μl 5x IT buffer, 2 μl insert-plasmid mastermix (containing 75 ng plasmid and an 8-fold molar excess of insert), 5.3 μl 1:1000 diluted T5 exonuclease (New England Biolabs M0363S, 10’000 U/ml), 1.6 μl of 1:10 diluted Phusion HF DNA polymerase (NEB M0530L, 2’000 U/ml), 1.3 μl Taq DNA ligase (NEB M0208L, 40’000 U/ml, undiluted) and H2 0 to a final volume of 13.5 μl. ..

    In Vitro:

    Article Title: A strategy of gene overexpression based on tandem repetitive promoters in Escherichia coli
    Article Snippet: The plasmid p5TG was linearized by Hind III to produce fragment 3. .. Then, fragment 1, 2 and 3 were assembled together in vitro under the action of T5 exonuclease (Epicentre), Phusion Hot Start DNA Polymerase (New England Biolabs (NEB)) and Taq DNA ligase (NEB) at 50°C for 15 min. .. The resulting constructs containing different promoters were then transformed into competent cells and were firstly screened based on the fluorescence signal and PCR detection.

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    New England Biolabs taq dna ligase
    Synthesis of a functional chloramphenicol acetyltransferase gene with changed codon composition. The ratio r of ‘active clones’ to ‘analyzed clones’ as described in the text is shown for different gene synthesis methods with or without an EMC step. A significant increase of r can be observed only in the cases where EMC is combined with an exonuclease activity present in the reaction or in the later amplification reaction. Prolonged incubation with E.coli endonuclease V results in no detectable product after the amplification steps (ss, single-stranded synthesis, ds, double-stranded synthesis; VII, T4 endonuclease VII; V, E.coli endonuclease V; T, <t>Taq</t> <t>DNA</t> polymerase; and Vn, Vent DNA polymerase).
    Taq Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/taq dna ligase/product/New England Biolabs
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    taq dna ligase - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

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    Synthesis of a functional chloramphenicol acetyltransferase gene with changed codon composition. The ratio r of ‘active clones’ to ‘analyzed clones’ as described in the text is shown for different gene synthesis methods with or without an EMC step. A significant increase of r can be observed only in the cases where EMC is combined with an exonuclease activity present in the reaction or in the later amplification reaction. Prolonged incubation with E.coli endonuclease V results in no detectable product after the amplification steps (ss, single-stranded synthesis, ds, double-stranded synthesis; VII, T4 endonuclease VII; V, E.coli endonuclease V; T, Taq DNA polymerase; and Vn, Vent DNA polymerase).

    Journal: Nucleic Acids Research

    Article Title: Removal of mismatched bases from synthetic genes by enzymatic mismatch cleavage

    doi: 10.1093/nar/gni058

    Figure Lengend Snippet: Synthesis of a functional chloramphenicol acetyltransferase gene with changed codon composition. The ratio r of ‘active clones’ to ‘analyzed clones’ as described in the text is shown for different gene synthesis methods with or without an EMC step. A significant increase of r can be observed only in the cases where EMC is combined with an exonuclease activity present in the reaction or in the later amplification reaction. Prolonged incubation with E.coli endonuclease V results in no detectable product after the amplification steps (ss, single-stranded synthesis, ds, double-stranded synthesis; VII, T4 endonuclease VII; V, E.coli endonuclease V; T, Taq DNA polymerase; and Vn, Vent DNA polymerase).

    Article Snippet: The reaction was denatured for 1 min at 95°C without enzyme, then cooled to 80°C for 1 min, after which 60 U of Taq DNA ligase (NEB) were added.

    Techniques: Functional Assay, Clone Assay, Activity Assay, Amplification, Incubation

    FEN1 protein facilitates cccDNA formation in vitro . (A) Schematic presentation of in vitro cccDNA formation assay. Purified NC-DNA (10 8 copies) was incubated with recombinant FEN1, Bst DNA polymerase, and Taq DNA ligase. Following incubation, the DNA was purified and analyzed (B–F). Regions for qPCR amplification (E and F) were indicated as p. The 5.4-kb PstI fragment in HBV plasmid (Control) has a partial HBV sequence but does not have core and intact P genes. (B) cccDNA-selective qPCR. Each result represents the mean ± SEM of three independent experiments. Asterisks indicate statistically significant differences; *** P

    Journal: PLoS Pathogens

    Article Title: Flap endonuclease 1 is involved in cccDNA formation in the hepatitis B virus

    doi: 10.1371/journal.ppat.1007124

    Figure Lengend Snippet: FEN1 protein facilitates cccDNA formation in vitro . (A) Schematic presentation of in vitro cccDNA formation assay. Purified NC-DNA (10 8 copies) was incubated with recombinant FEN1, Bst DNA polymerase, and Taq DNA ligase. Following incubation, the DNA was purified and analyzed (B–F). Regions for qPCR amplification (E and F) were indicated as p. The 5.4-kb PstI fragment in HBV plasmid (Control) has a partial HBV sequence but does not have core and intact P genes. (B) cccDNA-selective qPCR. Each result represents the mean ± SEM of three independent experiments. Asterisks indicate statistically significant differences; *** P

    Article Snippet: NC-DNA (108 copies) was incubated with 32 units (U) of Thermostable FEN1 in ThermoPol Buffer (New England Biolabs) at 65°C for 10 min, followed by incubation with 8 U of Bst DNA polymerase, 40 U of Taq DNA ligase, 100 μM dNTPs, and NAD+ (all from New England Biolabs).

    Techniques: In Vitro, Tube Formation Assay, Purification, Incubation, Recombinant, Real-time Polymerase Chain Reaction, Amplification, Plasmid Preparation, Sequencing

    Construction outline of the MCP tac s promoter clusters . Fragment 5CP tac s with the flanking sequence was amplified by PCR with p5TG as the template. Fragment 1 was generated by digesting fragment 5CP tac s with BamH I. Fragment 2 was digested from fragment 5CP tac s with BamH I and Hind III. Fragment 3 was linearized from the plasmid p5TG with Hind III. Then, the three fragments were assembled together under the action of T5 exonuclease, Phusion DNA polymerase and Taq DNA ligase in the isothermal process.

    Journal: Microbial Cell Factories

    Article Title: A strategy of gene overexpression based on tandem repetitive promoters in Escherichia coli

    doi: 10.1186/1475-2859-11-19

    Figure Lengend Snippet: Construction outline of the MCP tac s promoter clusters . Fragment 5CP tac s with the flanking sequence was amplified by PCR with p5TG as the template. Fragment 1 was generated by digesting fragment 5CP tac s with BamH I. Fragment 2 was digested from fragment 5CP tac s with BamH I and Hind III. Fragment 3 was linearized from the plasmid p5TG with Hind III. Then, the three fragments were assembled together under the action of T5 exonuclease, Phusion DNA polymerase and Taq DNA ligase in the isothermal process.

    Article Snippet: Then, fragment 1, 2 and 3 were assembled together in vitro under the action of T5 exonuclease (Epicentre), Phusion Hot Start DNA Polymerase (New England Biolabs (NEB)) and Taq DNA ligase (NEB) at 50°C for 15 min.

    Techniques: Sequencing, Amplification, Polymerase Chain Reaction, Generated, Plasmid Preparation

    In vitro construction of ADP fragment using overlap-extension PCR. ADP fragment was amplified by PCR through 32 cycles include denaturing step (95 °C for 45 s), annealing step (60 °C for 45 s) and elongation step (72 °C for 1 min). The two fragments were then purified and joined through 10 cycles of overlap-extension PCR include denaturing step at 95 °C for 45 s, annealing step at 60 °C for 45 s and elongating step at 72 °C for 1 min. L1 : PCR product of exon 2 (204 bp). L2 : PCR product of exon 3 (531 bp). L3 : the full length of ADP fragment (734 bp). M : 100 bp DNA marker.

    Journal: International Journal of Molecular Sciences

    Article Title: A Comparative Study on the Expression, Purification and Functional Characterization of Human Adiponectin in Pichia pastoris and Escherichia coli

    doi: 10.3390/ijms13033549

    Figure Lengend Snippet: In vitro construction of ADP fragment using overlap-extension PCR. ADP fragment was amplified by PCR through 32 cycles include denaturing step (95 °C for 45 s), annealing step (60 °C for 45 s) and elongation step (72 °C for 1 min). The two fragments were then purified and joined through 10 cycles of overlap-extension PCR include denaturing step at 95 °C for 45 s, annealing step at 60 °C for 45 s and elongating step at 72 °C for 1 min. L1 : PCR product of exon 2 (204 bp). L2 : PCR product of exon 3 (531 bp). L3 : the full length of ADP fragment (734 bp). M : 100 bp DNA marker.

    Article Snippet: The ligation mixture was prepared by adding digested vector and digested ADP fragment with DNA ligase and its suitable ligation buffer (New England Biolabs, UK).

    Techniques: In Vitro, Polymerase Chain Reaction, Amplification, Purification, Marker