m0202 (New England Biolabs)


Name:
T4 DNA Ligase
Description:
T4 DNA Ligase 100 000 units
Catalog Number:
m0202l
Price:
256
Category:
DNA Ligases
Size:
100 000 units
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Structured Review

T4 DNA Ligase 100 000 units
https://www.bioz.com/result/m0202/product/New England Biolabs
Average 99 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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Blocking Assay:Article Title: Blocking of targeted microRNAs from next-generation sequencing libraries Article Snippet: .. Because our approach relies on Ligation:Article Title: Efficient assembly of very short oligonucleotides using T4 DNA Ligase Article Snippet: Final solutions were washed twice with equal volume TE 10:1 (pH 7.5) to remove excess bottom strand and quantified using a standard PicoGreen fluorescence assay (Molecular Probes, Invitrogen Detection Technologies). .. Ligation assays 25 μL ligations contained 0.2 μM immobilized dsDNA on beads, 0.002 to 20 μM 5'-phosphorylated, 3'-FAM oligo, 20 units of Article Title: The longevity SNP rs2802292 uncovered: HSF1 activates stress-dependent expression of FOXO3 through an intronic enhancer Article Snippet: To further confirm this observation, we performed a ChIP-loop analysis of HEK-293 cells and primary human fibroblasts (GG and TT). .. H2 O2 -stressed cells were treated with formaldehyde to obtain cross-linked chromatin, after which DNA/protein complexes were first digested with the Csp6I restriction enzyme and then selected with anti-HSF1 antibodies; at last, ligation of chromatin fragments was carried out with the Article Title: A two-nuclease pathway involving RNase H1 is required for primer removal at human mitochondrial OriL Article Snippet: We focused only on T4 DNA ligase, since DNA ligase III activity was undetectable even with wild type RNase H1 as discussed above (Figure ). .. While wild type RNase H1 together with Produced:Article Title: A two-nuclease pathway involving RNase H1 is required for primer removal at human mitochondrial OriL Article Snippet: We focused only on T4 DNA ligase, since DNA ligase III activity was undetectable even with wild type RNase H1 as discussed above (Figure ). .. While wild type RNase H1 together with Mutagenesis:Article Title: A two-nuclease pathway involving RNase H1 is required for primer removal at human mitochondrial OriL Article Snippet: We focused only on T4 DNA ligase, since DNA ligase III activity was undetectable even with wild type RNase H1 as discussed above (Figure ). .. While wild type RNase H1 together with other:Article Title: A two-nuclease pathway involving RNase H1 is required for primer removal at human mitochondrial OriL Article Snippet: For this, we performed the POLRMT-primed minicircle assay with T4 DNA ligase, and then tested whether the closed circular products could be subsequently converted to nicked circles by KOH treatment ( ). Article Title: A junction branch point adjacent to a DNA backbone nick directs substrate cleavage by Saccharomyces cerevisiae Mus81-Mms4 Article Snippet: Incision that is not coincident with the junction branch point generates a small gapped region in the incision product, confirmed by the inability to ligate the resulting duplex with T4 DNA ligase. DNA Ligation:Article Title: Mechanical properties of DNA-like polymers Article Snippet: .. DNA ligase-catalyzed cyclization reactions were performed at ∼22°C with 1 nM DNA restriction fragment, T4 DNA ligation buffer (50 mM Tris–HCl, pH 7.5, 10 mM MgCl2 , 1 mM ATP, 10 mM dithiothreitol; NEB) and a final concentration of 100 U/ml Concentration Assay:Article Title: Mechanical properties of DNA-like polymers Article Snippet: .. DNA ligase-catalyzed cyclization reactions were performed at ∼22°C with 1 nM DNA restriction fragment, T4 DNA ligation buffer (50 mM Tris–HCl, pH 7.5, 10 mM MgCl2 , 1 mM ATP, 10 mM dithiothreitol; NEB) and a final concentration of 100 U/ml |