il 12 elisa kit  (Sino Biological)


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    Name:
    IL 12 ELISA Kit Human
    Description:
    For the quantitative determination of Human IL 12 IL12A IL12B Heterodimer concentration in cell culture supernates
    Catalog Number:
    kitct011
    Price:
    None
    Category:
    ELISA Kit
    Reactivity:
    Human
    Product Aliases:
    CLMF ELISA Kit Human, CLMF2 ELISA Kit Human, IL-12A ELISA Kit Human, IL-12B ELISA Kit Human, IL12 ELISA Kit Human, IL12 p70 ELISA Kit Human, IMD28 ELISA Kit Human, IMD29 ELISA Kit Human, NFSK ELISA Kit Human, NKSF ELISA Kit Human, NKSF1 ELISA Kit Human, NKSF2 ELISA Kit Human, P35 ELISA Kit Human
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    Structured Review

    Sino Biological il 12 elisa kit
    Peptides+HB 100-108 /pulsed DCs secreted abundant levels of proinflammatory cytokines. Immature moDCs were left untreated or pulsed with peptides±HB 100-108 or free HB 100-108 (each with 40 μ g/ml) for 1 hour at 37°C. Then, cells were washed and cultured for 24 hours, followed by detection of the secreted IL-6 (a), IFN- γ (b), and TNF- α (c) in the culture media by a cytometric bead array (CBA) kit. (d) <t>IL-12</t> was detected by ELISA after 48 hours. Results represent the mean ± SEM of three independent experiments.
    For the quantitative determination of Human IL 12 IL12A IL12B Heterodimer concentration in cell culture supernates
    https://www.bioz.com/result/il 12 elisa kit/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 12 elisa kit - by Bioz Stars, 2021-03
    93/100 stars

    Images

    1) Product Images from "Triggered Immune Response Induced by Antigenic Epitopes Covalently Linked with Immunoadjuvant-Pulsed Dendritic Cells as a Promising Cancer Vaccine"

    Article Title: Triggered Immune Response Induced by Antigenic Epitopes Covalently Linked with Immunoadjuvant-Pulsed Dendritic Cells as a Promising Cancer Vaccine

    Journal: Journal of Immunology Research

    doi: 10.1155/2020/3965061

    Peptides+HB 100-108 /pulsed DCs secreted abundant levels of proinflammatory cytokines. Immature moDCs were left untreated or pulsed with peptides±HB 100-108 or free HB 100-108 (each with 40 μ g/ml) for 1 hour at 37°C. Then, cells were washed and cultured for 24 hours, followed by detection of the secreted IL-6 (a), IFN- γ (b), and TNF- α (c) in the culture media by a cytometric bead array (CBA) kit. (d) IL-12 was detected by ELISA after 48 hours. Results represent the mean ± SEM of three independent experiments.
    Figure Legend Snippet: Peptides+HB 100-108 /pulsed DCs secreted abundant levels of proinflammatory cytokines. Immature moDCs were left untreated or pulsed with peptides±HB 100-108 or free HB 100-108 (each with 40 μ g/ml) for 1 hour at 37°C. Then, cells were washed and cultured for 24 hours, followed by detection of the secreted IL-6 (a), IFN- γ (b), and TNF- α (c) in the culture media by a cytometric bead array (CBA) kit. (d) IL-12 was detected by ELISA after 48 hours. Results represent the mean ± SEM of three independent experiments.

    Techniques Used: Cell Culture, Crocin Bleaching Assay, Enzyme-linked Immunosorbent Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Triggered Immune Response Induced by Antigenic Epitopes Covalently Linked with Immunoadjuvant-Pulsed Dendritic Cells as a Promising Cancer Vaccine
    Article Snippet: After one hour, pulsed DCs were washed and cultured for 48 hours. .. The secreted IL-12 was detected by a IL-12 ELISA kit (Sino Biological, China) according to the manufacturer's protocol. .. The optical density (OD) of samples was assessed at 550 nm using a microtiter plate spectrophotometer (Beckman Coulter detection platform, USA).

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  • 93
    Sino Biological il 12 elisa kit
    Peptides+HB 100-108 /pulsed DCs secreted abundant levels of proinflammatory cytokines. Immature moDCs were left untreated or pulsed with peptides±HB 100-108 or free HB 100-108 (each with 40 μ g/ml) for 1 hour at 37°C. Then, cells were washed and cultured for 24 hours, followed by detection of the secreted IL-6 (a), IFN- γ (b), and TNF- α (c) in the culture media by a cytometric bead array (CBA) kit. (d) <t>IL-12</t> was detected by ELISA after 48 hours. Results represent the mean ± SEM of three independent experiments.
    Il 12 Elisa Kit, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 12 elisa kit/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 12 elisa kit - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

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    Peptides+HB 100-108 /pulsed DCs secreted abundant levels of proinflammatory cytokines. Immature moDCs were left untreated or pulsed with peptides±HB 100-108 or free HB 100-108 (each with 40 μ g/ml) for 1 hour at 37°C. Then, cells were washed and cultured for 24 hours, followed by detection of the secreted IL-6 (a), IFN- γ (b), and TNF- α (c) in the culture media by a cytometric bead array (CBA) kit. (d) IL-12 was detected by ELISA after 48 hours. Results represent the mean ± SEM of three independent experiments.

    Journal: Journal of Immunology Research

    Article Title: Triggered Immune Response Induced by Antigenic Epitopes Covalently Linked with Immunoadjuvant-Pulsed Dendritic Cells as a Promising Cancer Vaccine

    doi: 10.1155/2020/3965061

    Figure Lengend Snippet: Peptides+HB 100-108 /pulsed DCs secreted abundant levels of proinflammatory cytokines. Immature moDCs were left untreated or pulsed with peptides±HB 100-108 or free HB 100-108 (each with 40 μ g/ml) for 1 hour at 37°C. Then, cells were washed and cultured for 24 hours, followed by detection of the secreted IL-6 (a), IFN- γ (b), and TNF- α (c) in the culture media by a cytometric bead array (CBA) kit. (d) IL-12 was detected by ELISA after 48 hours. Results represent the mean ± SEM of three independent experiments.

    Article Snippet: The secreted IL-12 was detected by a IL-12 ELISA kit (Sino Biological, China) according to the manufacturer's protocol.

    Techniques: Cell Culture, Crocin Bleaching Assay, Enzyme-linked Immunosorbent Assay