sars cov 2 np antigen  (Sino Biological)


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    Name:
    SARS CoV 2 2019 nCoV Nucleocapsid Detection ELISA Kit
    Description:
    The kit has been verified by high purity SARS CoV 2 2019 nCoV Nucleoprotein NP Protein Cat 40588 V08B It can recognize recombinant SARS CoV Nucleoprotein NP Protein His Tag Cat 40143 V08B but no react with recombinant MERS CoV Nucleoprotein NP protein His Tag Cat 40068 V08B
    Catalog Number:
    kit40588
    Price:
    None
    Category:
    ELISA Kit
    Reactivity:
    2019 nCoV
    Product Aliases:
    coronavirus NP ELISA Kit 2019-nCoV, coronavirus Nucleocapsid ELISA Kit 2019-nCoV, coronavirus Nucleoprotein ELISA Kit 2019-nCoV, cov np ELISA Kit 2019-nCoV, ncov NP ELISA Kit 2019-nCoV, NCP-CoV Nucleocapsid ELISA Kit 2019-nCoV, novel coronavirus NP ELISA Kit 2019-nCoV, novel coronavirus Nucleocapsid ELISA Kit 2019-nCoV, novel coronavirus Nucleoprotein ELISA Kit 2019-nCoV, np ELISA Kit 2019-nCoV, nucleocapsid ELISA Kit 2019-nCoV, Nucleoprotein ELISA Kit 2019-nCoV
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    Structured Review

    Sino Biological sars cov 2 np antigen
    In situ hybridization for severe acute respiratory syndrome coronavirus 2 <t>(SARS-CoV-2).</t> (a) Tissue quality was evaluated by performing RNAscope analysis for mRNA of the housekeeping gene peptidylprolyl isomerase B ( PPIB ). Positive cytoplasmic staining confirms adequate quality. Signal was detected using 3,3′-diaminobenzidine (DAB) (brown) chromogen. (periodic acid–Schiff counter stain; original magnification ×400). (b) RNAscope using probes directed against SARS-CoV-2 shows absence of signal in the patient's kidney parenchyma (periodic acid–Schiff counter stain; original magnification ×400).
    The kit has been verified by high purity SARS CoV 2 2019 nCoV Nucleoprotein NP Protein Cat 40588 V08B It can recognize recombinant SARS CoV Nucleoprotein NP Protein His Tag Cat 40143 V08B but no react with recombinant MERS CoV Nucleoprotein NP protein His Tag Cat 40068 V08B
    https://www.bioz.com/result/sars cov 2 np antigen/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 np antigen - by Bioz Stars, 2021-03
    95/100 stars

    Images

    1) Product Images from "Collapsing Glomerulopathy in a Patient With Coronavirus Disease 2019 (COVID-19)"

    Article Title: Collapsing Glomerulopathy in a Patient With Coronavirus Disease 2019 (COVID-19)

    Journal: Kidney International Reports

    doi: 10.1016/j.ekir.2020.04.002

    In situ hybridization for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). (a) Tissue quality was evaluated by performing RNAscope analysis for mRNA of the housekeeping gene peptidylprolyl isomerase B ( PPIB ). Positive cytoplasmic staining confirms adequate quality. Signal was detected using 3,3′-diaminobenzidine (DAB) (brown) chromogen. (periodic acid–Schiff counter stain; original magnification ×400). (b) RNAscope using probes directed against SARS-CoV-2 shows absence of signal in the patient's kidney parenchyma (periodic acid–Schiff counter stain; original magnification ×400).
    Figure Legend Snippet: In situ hybridization for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). (a) Tissue quality was evaluated by performing RNAscope analysis for mRNA of the housekeeping gene peptidylprolyl isomerase B ( PPIB ). Positive cytoplasmic staining confirms adequate quality. Signal was detected using 3,3′-diaminobenzidine (DAB) (brown) chromogen. (periodic acid–Schiff counter stain; original magnification ×400). (b) RNAscope using probes directed against SARS-CoV-2 shows absence of signal in the patient's kidney parenchyma (periodic acid–Schiff counter stain; original magnification ×400).

    Techniques Used: In Situ Hybridization, Staining

    Related Articles

    Immunohistochemistry:

    Article Title: Collapsing Glomerulopathy in a Patient With Coronavirus Disease 2019 (COVID-19)
    Article Snippet: A recent report of postmortem kidney tissue from 6 patients who died of COVID-19 showed acute tubular injury without glomerular abnormalities. .. The SARS-CoV-2 NP antigen was described in renal tubules by immunohistochemical analysis in this report when the renal tissue was reacted with a rabbit monoclonal antibody directed against the SARS-CoV-2 nucleoprotein (Clone ID: 019; Sino Biological, Beijing, China). .. In our laboratory, immunohistochemical analysis of renal tissue using this antibody under numerous conditions showed nonspecific positive staining in the renal parenchyma of all kidneys.

    Infection:

    Article Title: Proteomics of SARS-CoV-2-infected host cells reveals therapy targets.
    Article Snippet: For dose–response curves, data were fitted with all replicates using OriginPro 2020 with the following equation: y A A A = 1 + 2 − 1 1 + 10 x x p(log − )0 IC50 values were generated by Origin together with metrics for curve fits. .. Detection of the nucleoprotein of SARS-CoV-2Viral infection was assessed by staining of SARS-CoV-2 nucleoprotein. .. In brief, cells were fixed with acetone:methanol (40:60) solution and immunostaining was performed using a monoclonal antibody directed against the nucleoprotein of SARS-CoV-2 (1:500, Sinobiological, 40143-R019-100ul), which was detected with a peroxidase-conjugated anti-rabbit secondary antibody (1:1,000, Dianova), followed by addition of AEC substrate.

    Staining:

    Article Title: Proteomics of SARS-CoV-2-infected host cells reveals therapy targets.
    Article Snippet: For dose–response curves, data were fitted with all replicates using OriginPro 2020 with the following equation: y A A A = 1 + 2 − 1 1 + 10 x x p(log − )0 IC50 values were generated by Origin together with metrics for curve fits. .. Detection of the nucleoprotein of SARS-CoV-2Viral infection was assessed by staining of SARS-CoV-2 nucleoprotein. .. In brief, cells were fixed with acetone:methanol (40:60) solution and immunostaining was performed using a monoclonal antibody directed against the nucleoprotein of SARS-CoV-2 (1:500, Sinobiological, 40143-R019-100ul), which was detected with a peroxidase-conjugated anti-rabbit secondary antibody (1:1,000, Dianova), followed by addition of AEC substrate.

    Enzyme-linked Immunosorbent Assay:

    Article Title: An hACE2 peptide mimic blocks SARS-CoV-2 Pulmonary Cell Infection
    Article Snippet: After 30 min at room temperature, cells were infected with 0.1 moi (VeroE6) or 0.3 moi (Calu3) of SRAS-Cov2 (SABA95 P2 stock) in infectious media. .. Cell supernatants were collected at 48 h post-infection for Elisa assay using SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP ELISA Kit from Sino biological, according the manufacturer’s instruction, and standard plaque assay. .. 4 Toxicity study on Vero-E6 cells and Calu3Cell viability was measured by MTT assays after treatment with 0, 0.1, 1.0 or 10 µM of the indicated peptide for 24, 48, or 72 h. Methodology: 96-well plates were used to seed 104 CALU-3 or Vero-E6 cells per well, which were let to adhere.

    Plaque Assay:

    Article Title: An hACE2 peptide mimic blocks SARS-CoV-2 Pulmonary Cell Infection
    Article Snippet: After 30 min at room temperature, cells were infected with 0.1 moi (VeroE6) or 0.3 moi (Calu3) of SRAS-Cov2 (SABA95 P2 stock) in infectious media. .. Cell supernatants were collected at 48 h post-infection for Elisa assay using SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP ELISA Kit from Sino biological, according the manufacturer’s instruction, and standard plaque assay. .. 4 Toxicity study on Vero-E6 cells and Calu3Cell viability was measured by MTT assays after treatment with 0, 0.1, 1.0 or 10 µM of the indicated peptide for 24, 48, or 72 h. Methodology: 96-well plates were used to seed 104 CALU-3 or Vero-E6 cells per well, which were let to adhere.

    Imaging:

    Article Title: Anti-SARS-CoV-2 activity of Andrographis paniculata extract and its major component Andrographolide in human lung epithelial cells and cytotoxicity evaluation in major organ cell representatives
    Article Snippet: Infected cells were then maintained at 37°C in 5% CO2 incubator for 48 h. Positive convalescent serum (heat-inactivated at 56°C for 30 min) of a COVID-19 patient and anti-human IgG (Santa Cruz Biotechnology, Dallas, TX, USA) were used for a viral inhibition as positive control and negative control, respectively. .. High-content imaging for SARS-CoV-2 nucleoprotein detection The cells in the 96-well plate were fixed and permeabilized with 50% (v/v) acetone in methanol on ice for 20 min. ..

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  • 95
    Sino Biological sars cov 2 np antigen
    In situ hybridization for severe acute respiratory syndrome coronavirus 2 <t>(SARS-CoV-2).</t> (a) Tissue quality was evaluated by performing RNAscope analysis for mRNA of the housekeeping gene peptidylprolyl isomerase B ( PPIB ). Positive cytoplasmic staining confirms adequate quality. Signal was detected using 3,3′-diaminobenzidine (DAB) (brown) chromogen. (periodic acid–Schiff counter stain; original magnification ×400). (b) RNAscope using probes directed against SARS-CoV-2 shows absence of signal in the patient's kidney parenchyma (periodic acid–Schiff counter stain; original magnification ×400).
    Sars Cov 2 Np Antigen, supplied by Sino Biological, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sars cov 2 np antigen/product/Sino Biological
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sars cov 2 np antigen - by Bioz Stars, 2021-03
    95/100 stars
      Buy from Supplier

    Image Search Results


    In situ hybridization for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). (a) Tissue quality was evaluated by performing RNAscope analysis for mRNA of the housekeeping gene peptidylprolyl isomerase B ( PPIB ). Positive cytoplasmic staining confirms adequate quality. Signal was detected using 3,3′-diaminobenzidine (DAB) (brown) chromogen. (periodic acid–Schiff counter stain; original magnification ×400). (b) RNAscope using probes directed against SARS-CoV-2 shows absence of signal in the patient's kidney parenchyma (periodic acid–Schiff counter stain; original magnification ×400).

    Journal: Kidney International Reports

    Article Title: Collapsing Glomerulopathy in a Patient With Coronavirus Disease 2019 (COVID-19)

    doi: 10.1016/j.ekir.2020.04.002

    Figure Lengend Snippet: In situ hybridization for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). (a) Tissue quality was evaluated by performing RNAscope analysis for mRNA of the housekeeping gene peptidylprolyl isomerase B ( PPIB ). Positive cytoplasmic staining confirms adequate quality. Signal was detected using 3,3′-diaminobenzidine (DAB) (brown) chromogen. (periodic acid–Schiff counter stain; original magnification ×400). (b) RNAscope using probes directed against SARS-CoV-2 shows absence of signal in the patient's kidney parenchyma (periodic acid–Schiff counter stain; original magnification ×400).

    Article Snippet: The SARS-CoV-2 NP antigen was described in renal tubules by immunohistochemical analysis in this report when the renal tissue was reacted with a rabbit monoclonal antibody directed against the SARS-CoV-2 nucleoprotein (Clone ID: 019; Sino Biological, Beijing, China).

    Techniques: In Situ Hybridization, Staining