human tnf α  (Sino Biological)


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    Name:
    TNF alpha ELISA Kit Human
    Description:
    For the quantitative determination of Human TNF alpha concentration in cell culture supernates
    Catalog Number:
    kit10602
    Price:
    None
    Category:
    ELISA Kit
    Reactivity:
    Human
    Product Aliases:
    DIF ELISA Kit Human, TNF-alpha ELISA Kit Human, TNFA ELISA Kit Human, TNFSF2 ELISA Kit Human
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    Structured Review

    Sino Biological human tnf α
    miR-155 inhibitor reduces the production of proinflammatory cytokines induced by LPS. ( A ) Fold changes in miR-155 expression were determined by RT-qPCR 24 h post transfection with 50 nM miRNA inhibitor control, 25 or 50 nM miR-155 inhibitor; ( B ) and ( C ) Expression of <t>TNF-α</t> and IL-1β in supernatant of PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 5 nM miR-155 inhibitor or 10 nM miR-155 inhibitor. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 10 nM miRNA inhibitor control, 5 nM miR-155 inhibitor or 10 nM miRNA inhibitor. All results are the average of three independent experiments. Statistical significance is shown.
    For the quantitative determination of Human TNF alpha concentration in cell culture supernates
    https://www.bioz.com/result/human tnf α/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human tnf α - by Bioz Stars, 2021-03
    91/100 stars

    Images

    1) Product Images from "Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-? and IL-1? in PBMCs"

    Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-? and IL-1? in PBMCs

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms141223910

    miR-155 inhibitor reduces the production of proinflammatory cytokines induced by LPS. ( A ) Fold changes in miR-155 expression were determined by RT-qPCR 24 h post transfection with 50 nM miRNA inhibitor control, 25 or 50 nM miR-155 inhibitor; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 5 nM miR-155 inhibitor or 10 nM miR-155 inhibitor. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 10 nM miRNA inhibitor control, 5 nM miR-155 inhibitor or 10 nM miRNA inhibitor. All results are the average of three independent experiments. Statistical significance is shown.
    Figure Legend Snippet: miR-155 inhibitor reduces the production of proinflammatory cytokines induced by LPS. ( A ) Fold changes in miR-155 expression were determined by RT-qPCR 24 h post transfection with 50 nM miRNA inhibitor control, 25 or 50 nM miR-155 inhibitor; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 5 nM miR-155 inhibitor or 10 nM miR-155 inhibitor. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 10 nM miRNA inhibitor control, 5 nM miR-155 inhibitor or 10 nM miRNA inhibitor. All results are the average of three independent experiments. Statistical significance is shown.

    Techniques Used: Expressing, Quantitative RT-PCR, Transfection, Enzyme-linked Immunosorbent Assay

    miR-155 upregulation correlates with increased production of TNF-α and IL-1β in Rheumatoid arthritis (RA) patients. ( A ) and ( B ): Increased expression of TNF-α and IL-1β in RA plasma. Results are expressed as pg/mL by an ELISA test; ( C ): MiR-155 is upregulated in PBMCs of active RA patients. Results in patients with RA ( n = 45) and OA ( n = 32) are shown as fold increase relative to the control group ( n = 25); ( D ) and ( E ): Correlations between Relative miR-155 expression with the TNF-α or IL-1β expression. RA, rheumatoid arthritis; OA, osteoarthritis. Statistical significance was considered with a p value
    Figure Legend Snippet: miR-155 upregulation correlates with increased production of TNF-α and IL-1β in Rheumatoid arthritis (RA) patients. ( A ) and ( B ): Increased expression of TNF-α and IL-1β in RA plasma. Results are expressed as pg/mL by an ELISA test; ( C ): MiR-155 is upregulated in PBMCs of active RA patients. Results in patients with RA ( n = 45) and OA ( n = 32) are shown as fold increase relative to the control group ( n = 25); ( D ) and ( E ): Correlations between Relative miR-155 expression with the TNF-α or IL-1β expression. RA, rheumatoid arthritis; OA, osteoarthritis. Statistical significance was considered with a p value

    Techniques Used: Expressing, Enzyme-linked Immunosorbent Assay

    miR-155 promotes production of TNF-α and IL-1β in human PBMCs. ( A ) Fold changes of miR-155 expression were determined by qRT-PCR after the transfection of miR-control, or miR-155 mimics at 25 or 50 nM for 24 h; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h post LPS treatment, or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. All results are the average of three independent experiments. Statistical significance is shown.
    Figure Legend Snippet: miR-155 promotes production of TNF-α and IL-1β in human PBMCs. ( A ) Fold changes of miR-155 expression were determined by qRT-PCR after the transfection of miR-control, or miR-155 mimics at 25 or 50 nM for 24 h; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h post LPS treatment, or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. All results are the average of three independent experiments. Statistical significance is shown.

    Techniques Used: Expressing, Quantitative RT-PCR, Transfection, Enzyme-linked Immunosorbent Assay

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    Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-? and IL-1? in PBMCs
    Article Snippet: .. The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China). .. Isolation, Culturing and Treatment of PBMCs Venous blood samples for PBMCs isolation were donated from healthy adults without acute infection.

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  • 91
    Sino Biological human tnf α
    miR-155 inhibitor reduces the production of proinflammatory cytokines induced by LPS. ( A ) Fold changes in miR-155 expression were determined by RT-qPCR 24 h post transfection with 50 nM miRNA inhibitor control, 25 or 50 nM miR-155 inhibitor; ( B ) and ( C ) Expression of <t>TNF-α</t> and IL-1β in supernatant of PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 5 nM miR-155 inhibitor or 10 nM miR-155 inhibitor. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 10 nM miRNA inhibitor control, 5 nM miR-155 inhibitor or 10 nM miRNA inhibitor. All results are the average of three independent experiments. Statistical significance is shown.
    Human Tnf α, supplied by Sino Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human tnf α/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human tnf α - by Bioz Stars, 2021-03
    91/100 stars
      Buy from Supplier

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    miR-155 inhibitor reduces the production of proinflammatory cytokines induced by LPS. ( A ) Fold changes in miR-155 expression were determined by RT-qPCR 24 h post transfection with 50 nM miRNA inhibitor control, 25 or 50 nM miR-155 inhibitor; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 5 nM miR-155 inhibitor or 10 nM miR-155 inhibitor. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 10 nM miRNA inhibitor control, 5 nM miR-155 inhibitor or 10 nM miRNA inhibitor. All results are the average of three independent experiments. Statistical significance is shown.

    Journal: International Journal of Molecular Sciences

    Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-? and IL-1? in PBMCs

    doi: 10.3390/ijms141223910

    Figure Lengend Snippet: miR-155 inhibitor reduces the production of proinflammatory cytokines induced by LPS. ( A ) Fold changes in miR-155 expression were determined by RT-qPCR 24 h post transfection with 50 nM miRNA inhibitor control, 25 or 50 nM miR-155 inhibitor; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 5 nM miR-155 inhibitor or 10 nM miR-155 inhibitor. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment, or (and) 24 h post transfection with 10 nM miRNA inhibitor control, 5 nM miR-155 inhibitor or 10 nM miRNA inhibitor. All results are the average of three independent experiments. Statistical significance is shown.

    Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

    Techniques: Expressing, Quantitative RT-PCR, Transfection, Enzyme-linked Immunosorbent Assay

    miR-155 upregulation correlates with increased production of TNF-α and IL-1β in Rheumatoid arthritis (RA) patients. ( A ) and ( B ): Increased expression of TNF-α and IL-1β in RA plasma. Results are expressed as pg/mL by an ELISA test; ( C ): MiR-155 is upregulated in PBMCs of active RA patients. Results in patients with RA ( n = 45) and OA ( n = 32) are shown as fold increase relative to the control group ( n = 25); ( D ) and ( E ): Correlations between Relative miR-155 expression with the TNF-α or IL-1β expression. RA, rheumatoid arthritis; OA, osteoarthritis. Statistical significance was considered with a p value

    Journal: International Journal of Molecular Sciences

    Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-? and IL-1? in PBMCs

    doi: 10.3390/ijms141223910

    Figure Lengend Snippet: miR-155 upregulation correlates with increased production of TNF-α and IL-1β in Rheumatoid arthritis (RA) patients. ( A ) and ( B ): Increased expression of TNF-α and IL-1β in RA plasma. Results are expressed as pg/mL by an ELISA test; ( C ): MiR-155 is upregulated in PBMCs of active RA patients. Results in patients with RA ( n = 45) and OA ( n = 32) are shown as fold increase relative to the control group ( n = 25); ( D ) and ( E ): Correlations between Relative miR-155 expression with the TNF-α or IL-1β expression. RA, rheumatoid arthritis; OA, osteoarthritis. Statistical significance was considered with a p value

    Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay

    miR-155 promotes production of TNF-α and IL-1β in human PBMCs. ( A ) Fold changes of miR-155 expression were determined by qRT-PCR after the transfection of miR-control, or miR-155 mimics at 25 or 50 nM for 24 h; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h post LPS treatment, or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. All results are the average of three independent experiments. Statistical significance is shown.

    Journal: International Journal of Molecular Sciences

    Article Title: Rheumatoid Arthritis-Associated MicroRNA-155 Targets SOCS1 and Upregulates TNF-? and IL-1? in PBMCs

    doi: 10.3390/ijms141223910

    Figure Lengend Snippet: miR-155 promotes production of TNF-α and IL-1β in human PBMCs. ( A ) Fold changes of miR-155 expression were determined by qRT-PCR after the transfection of miR-control, or miR-155 mimics at 25 or 50 nM for 24 h; ( B ) and ( C ) Expression of TNF-α and IL-1β in supernatant of PBMCs 24 h post LPS treatment, or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. Results were expressed as pg/mL by an ELISA test; and ( D ) mRNA expression of TNF-α (group 1) and IL-1β (group 2) in PBMCs 24 h after LPS treatment or 24 h post transfection with 25 nM miR-155 mimics, 50 nM miR-155 mimics or 50 nM miRNA control. All results are the average of three independent experiments. Statistical significance is shown.

    Article Snippet: The levels of TNF-α and IL-1β in plasma and PBMCs supernatant were measured by an enzyme immunoassay using the human TNF-α and IL-1β ELISA Kit (Sino Biological Inc., Beijing, China).

    Techniques: Expressing, Quantitative RT-PCR, Transfection, Enzyme-linked Immunosorbent Assay