supernuclease (Sino Biological)
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Structured Review
Sino Biological
supernuclease
Supernuclease, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/supernuclease/product/Sino Biological
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Supernuclease, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/supernuclease/product/Sino Biological
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
supernuclease - by Bioz Stars,
2021-05
93/100 stars
Images
1) Product Images from "Rapid porcine corneal decellularization through the use of sodium N-lauroyl glutamate and supernuclease"
Article Title: Rapid porcine corneal decellularization through the use of sodium N-lauroyl glutamate and supernuclease
Journal: Journal of Tissue Engineering
doi: 10.1177/2041731419875876
Figure Legend Snippet: Cytotoxicity, cytocompatibility, and biocompatibility examinations of porcine corneal stroma decellularized with SLG and supernuclease: (a) The proliferation curve of HECEs during 6 days’ cultivation in normal or conditioned culture medium ( n = 3 per group). (b) SEM micrographs of HCECs at 3 days after the cultivation on the surface of a DPC ( n = 3). (c) Rabbit corneal micropocket implantation results and (d) rabbit anterior chamber implantation results at 10 days postoperation ( n = 3 per group).
Techniques Used:
Figure Legend Snippet: Lamellar transplantation with porcine corneal stroma decellularized with SLG and supernuclease in rabbits: (a) Corneal re-epithelialization results detected by fluorescence staining at 1, 7, and 9 days after transplantation. (b) General appearance of eyes examined by slit lamp at 1, 7, and 28 days after transplantation. (c) OCT photographs of normal and decellularized corneal stroma at 7 days after transplantation. (d) H E staining of the DPCs at 14 and 28 days after transplantation. (e) Confocal microscopy photographs of DPCs at 4 months after transplantation.
Techniques Used: Transplantation Assay, Fluorescence, Staining, Confocal Microscopy
2) Product Images from "ATPase activity tightly regulates RecA nucleofilaments to promote homologous recombination"
Article Title: ATPase activity tightly regulates RecA nucleofilaments to promote homologous recombination
Journal: Cell Discovery
doi: 10.1038/celldisc.2016.53
Figure Legend Snippet: ATP hydrolysis inhibits the formation of the RecA-saturated nucleofilaments and increases the dynamics from nucleation to extension. ( a ) CE-LIF electropherograms of the reaction mixtures of TMR-T 18 (10 n M ) and RecA protein (16.0 μ M ), showing the formation of six distinct RecA-TMR-T 18 complexes induced by ATP hydrolysis. The Y axis of the bottom trace can be applied to all stacked traces in the panel. ( b ) CE-LIFP electropherograms of the reaction mixtures of TMR-ss83mer (10 n M ) and RecA (3.0 μ M ), showing the formation of three types of RecA nucleofilaments in the presence of ATP. Peaks 1–3 represent the lightly assembled, moderately assembled and RecA-saturated nucleofilaments, respectively. The Y axis of the bottom trace can be applied to all stacked traces in the panel. ( c ) The relative abundance of the three types of RecA-TMR-ss83mer filaments is dependent on ATP concentration. ( d ) Denaturing PAGE analysis of time-dependent products of supernuclease digestion of the Cy5-ss83mer (20 n M ) and the reaction mixtures of Cy5-ss83mer (20 n M ), RecA (3.0 μ M ) and one nucleotide cofactor as indicated. M indicates ssDNA size markers with a unit of nucleotides (nts). The reactions proceeded at 37 °C for 10 min.
Techniques Used: Concentration Assay, Polyacrylamide Gel Electrophoresis
Related Articles
Centrifugation:Article Title: Elongation/Termination Factor Exchange Mediated by PP1 Phosphatase Orchestrates Transcription Termination Article Snippet: Expression was carried out in Escherichia coli BL21 strain grown to 0.8 OD at 37°C and induced with 1mM IPTG for 3h. .. Cells from 12L were collected by centrifugation at 6000 g, 4°C for 15 min. Pellets were resuspended in NiTA buffer (50 mM Tris-HCl pH 7.8, 500 mM NaCl, 5 mM imidazole, 1 mM 2-mercaptoethanol) supplemented with 2500 U of other:Article Title: Rapid porcine corneal decellularization through the use of sodium N-lauroyl glutamate and supernuclease Article Snippet: Preparation of DPCIn order to compare the decellularization effects of detergents, the porcine corneal stroma was separately treated with 0.5% SLG (Sigma-Aldrich, St. Louis, MO, USA), SLS (Sigma-Aldrich), SDS (Solarbio, Beijing, China), or Triton X-100 (Solarbio), supplemented with 200 U/mL Protein Purification:Article Title: Transport of DNA within cohesin involves clamping on top of engaged heads by Scc2 and entrapment within the ring by Scc3 Article Snippet: Cells were then harvested by centrifugation at 1000 g, washed with PBS, and then frozen in liquid nitrogen and stored at −80°C. .. Protein purification Cells were thawed in Buffer A (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP (Thermo Fisher), 5% glycerol) supplemented with 1 Complete Protease Inhibitor (EDTA-free) tablet (Roche), 70 µg RNAse A (Roche), and 100 U ml−1 Article Title: ATP dependent DNA transport within cohesin: Scc2 clamps DNA on top of engaged heads while Scc3 promotes entrapment within the SMC-kleisin ring Article Snippet: Cells were then harvested by centrifugation at 1000g, washed with PBS, and then frozen in liquid nitrogen and stored at −80°C. .. Protein purification Cells were thawed in Buffer A (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP (Thermo Fisher), 5% glycerol) supplemented with 1 Complete Protease Inhibitor (EDTA-free) tablet (Roche), 70 µg RNAse A (Roche), and 100 U ml-1 Protease Inhibitor:Article Title: Transport of DNA within cohesin involves clamping on top of engaged heads by Scc2 and entrapment within the ring by Scc3 Article Snippet: Cells were then harvested by centrifugation at 1000 g, washed with PBS, and then frozen in liquid nitrogen and stored at −80°C. .. Protein purification Cells were thawed in Buffer A (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP (Thermo Fisher), 5% glycerol) supplemented with 1 Complete Protease Inhibitor (EDTA-free) tablet (Roche), 70 µg RNAse A (Roche), and 100 U ml−1 Article Title: ATP dependent DNA transport within cohesin: Scc2 clamps DNA on top of engaged heads while Scc3 promotes entrapment within the SMC-kleisin ring Article Snippet: Cells were then harvested by centrifugation at 1000g, washed with PBS, and then frozen in liquid nitrogen and stored at −80°C. .. Protein purification Cells were thawed in Buffer A (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP (Thermo Fisher), 5% glycerol) supplemented with 1 Complete Protease Inhibitor (EDTA-free) tablet (Roche), 70 µg RNAse A (Roche), and 100 U ml-1 Article Title: Cellular N-myristoyltransferases play a crucial picornavirus genus-specific role in viral assembly, virion maturation, and infectivity Article Snippet: NP-40 and Tris were purchased from AppliChem, SDS was from Serva, D-sucrose from Roth; other chemicals were obtained from Sigma-Aldrich unless otherwise stated. .. DNase I, RNase A and EDTA-free complete protease inhibitor cocktail were from Roche, Sonication:Article Title: Transport of DNA within cohesin involves clamping on top of engaged heads by Scc2 and entrapment within the ring by Scc3 Article Snippet: Cells were then harvested by centrifugation at 1000 g, washed with PBS, and then frozen in liquid nitrogen and stored at −80°C. .. Protein purification Cells were thawed in Buffer A (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP (Thermo Fisher), 5% glycerol) supplemented with 1 Complete Protease Inhibitor (EDTA-free) tablet (Roche), 70 µg RNAse A (Roche), and 100 U ml−1 Article Title: ATP dependent DNA transport within cohesin: Scc2 clamps DNA on top of engaged heads while Scc3 promotes entrapment within the SMC-kleisin ring Article Snippet: Cells were then harvested by centrifugation at 1000g, washed with PBS, and then frozen in liquid nitrogen and stored at −80°C. .. Protein purification Cells were thawed in Buffer A (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM TCEP (Thermo Fisher), 5% glycerol) supplemented with 1 Complete Protease Inhibitor (EDTA-free) tablet (Roche), 70 µg RNAse A (Roche), and 100 U ml-1 |