supernuclease (Sino Biological)


Structured Review

Supernuclease, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/supernuclease/product/Sino Biological
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
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Images
1) Product Images from "ATPase activity tightly regulates RecA nucleofilaments to promote homologous recombination"
Article Title: ATPase activity tightly regulates RecA nucleofilaments to promote homologous recombination
Journal: Cell Discovery
doi: 10.1038/celldisc.2016.53

Figure Legend Snippet: ATP hydrolysis inhibits the formation of the RecA-saturated nucleofilaments and increases the dynamics from nucleation to extension. ( a ) CE-LIF electropherograms of the reaction mixtures of TMR-T 18 (10 n M ) and RecA protein (16.0 μ M ), showing the formation of six distinct RecA-TMR-T 18 complexes induced by ATP hydrolysis. The Y axis of the bottom trace can be applied to all stacked traces in the panel. ( b ) CE-LIFP electropherograms of the reaction mixtures of TMR-ss83mer (10 n M ) and RecA (3.0 μ M ), showing the formation of three types of RecA nucleofilaments in the presence of ATP. Peaks 1–3 represent the lightly assembled, moderately assembled and RecA-saturated nucleofilaments, respectively. The Y axis of the bottom trace can be applied to all stacked traces in the panel. ( c ) The relative abundance of the three types of RecA-TMR-ss83mer filaments is dependent on ATP concentration. ( d ) Denaturing PAGE analysis of time-dependent products of supernuclease digestion of the Cy5-ss83mer (20 n M ) and the reaction mixtures of Cy5-ss83mer (20 n M ), RecA (3.0 μ M ) and one nucleotide cofactor as indicated. M indicates ssDNA size markers with a unit of nucleotides (nts). The reactions proceeded at 37 °C for 10 min.
Techniques Used: Concentration Assay, Polyacrylamide Gel Electrophoresis
2) Product Images from "Rapid porcine corneal decellularization through the use of sodium N-lauroyl glutamate and supernuclease"
Article Title: Rapid porcine corneal decellularization through the use of sodium N-lauroyl glutamate and supernuclease
Journal: Journal of Tissue Engineering
doi: 10.1177/2041731419875876

Figure Legend Snippet: Cytotoxicity, cytocompatibility, and biocompatibility examinations of porcine corneal stroma decellularized with SLG and supernuclease: (a) The proliferation curve of HECEs during 6 days’ cultivation in normal or conditioned culture medium ( n = 3 per group). (b) SEM micrographs of HCECs at 3 days after the cultivation on the surface of a DPC ( n = 3). (c) Rabbit corneal micropocket implantation results and (d) rabbit anterior chamber implantation results at 10 days postoperation ( n = 3 per group).
Techniques Used:

Figure Legend Snippet: Lamellar transplantation with porcine corneal stroma decellularized with SLG and supernuclease in rabbits: (a) Corneal re-epithelialization results detected by fluorescence staining at 1, 7, and 9 days after transplantation. (b) General appearance of eyes examined by slit lamp at 1, 7, and 28 days after transplantation. (c) OCT photographs of normal and decellularized corneal stroma at 7 days after transplantation. (d) H E staining of the DPCs at 14 and 28 days after transplantation. (e) Confocal microscopy photographs of DPCs at 4 months after transplantation.
Techniques Used: Transplantation Assay, Fluorescence, Staining, Confocal Microscopy
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