azure c600 imaging system  (Azure Biosystems)


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    Azure Biosystems azure c600 imaging system
    Azure C600 Imaging System, supplied by Azure Biosystems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/azure c600 imaging system/product/Azure Biosystems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    azure c600 imaging system - by Bioz Stars, 2021-09
    99/100 stars

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    Related Articles

    Incubation:

    Article Title: Plasmodium falciparum guanylyl cyclase-alpha and the activity of its appended P4-ATPase domain are essential for cGMP synthesis and blood stage egress
    Article Snippet: .. Following incubation with secondary antibodies conjugated to near infrared (NIR) dyes, washed membranes were dried between Whatman 3MM blotting papers and imaged using an Azure c600 Imaging System (Azure Biosystems, California, United States) or a ChemiDoc Imaging System (Bio-Rad). ..

    Imaging:

    Article Title: Plasmodium falciparum guanylyl cyclase-alpha and the activity of its appended P4-ATPase domain are essential for cGMP synthesis and blood stage egress
    Article Snippet: .. Following incubation with secondary antibodies conjugated to near infrared (NIR) dyes, washed membranes were dried between Whatman 3MM blotting papers and imaged using an Azure c600 Imaging System (Azure Biosystems, California, United States) or a ChemiDoc Imaging System (Bio-Rad). ..

    Article Title: Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.
    Article Snippet: .. Hidradenitis suppurativa (HS), also known as acne inversa, is an incapacitating skin disorder of unknown etiology manifested as abscess-like nodules and boils resulting in fistulas and tissue scarring as it progresses. ..

    Article Title: A Role for Nrf2 Expression in Defining the Aging of Hippocampal Neural Stem Cells
    Article Snippet: .. The relative intensity of the bands was visualized using SuperSignal West Femto Max Sensitivity Substrate (ThermoFisher Scientific, Waltham, MA, USA) on an Azure c600 imaging system (Azure Biosystems, Dublin, CA, USA). ..

    Article Title: Restricted Water Intake Adversely Affects Rat Vocal Fold Biology.
    Article Snippet: .. A holistic understanding of the many ways that systemic dehydration affects vocal fold biology is still evolving. ..

    Article Title: Rapid degradation of progressive ankylosis protein (ANKH) in craniometaphyseal dysplasia
    Article Snippet: .. Protein bands were detected by enhanced chemiluminescent detection reagent (Azure Biosystems) and visualized by an Azure c600 imaging system (Azure Biosystems). ..

    Article Title: AfsK-Mediated Site-Specific Phosphorylation Regulates DnaA Initiator Protein Activity in Streptomyces coelicolor
    Article Snippet: .. The reaction products were subjected to 3.5% native PAGE at 4°C for 6 h. Near-infrared signals were detected using an Azure c600 imaging system (Azure Biosystems). ..

    Article Title: Enhancing Recombinant Protein Yields in the E. coli Periplasm by Combining Signal Peptide and Production Rate Screening
    Article Snippet: .. Chemiluminescence and fluorescence signals were detected using an Azure c600 imaging system (Azure Biosystems). ..

    Article Title: Icariin, but Not Genistein, Exerts Osteogenic and Anti-apoptotic Effects in Osteoblastic Cells by Selective Activation of Non-genomic ERα Signaling
    Article Snippet: .. The chemiluminescence intensity of each band was measured by Azure c600 imaging system (Azure Biosystems, Dublin, CA, United States) and quantified using ImageJ program (NIH, Bethesda, MD, United States). ..

    Clear Native PAGE:

    Article Title: AfsK-Mediated Site-Specific Phosphorylation Regulates DnaA Initiator Protein Activity in Streptomyces coelicolor
    Article Snippet: .. The reaction products were subjected to 3.5% native PAGE at 4°C for 6 h. Near-infrared signals were detected using an Azure c600 imaging system (Azure Biosystems). ..

    Fluorescence:

    Article Title: Enhancing Recombinant Protein Yields in the E. coli Periplasm by Combining Signal Peptide and Production Rate Screening
    Article Snippet: .. Chemiluminescence and fluorescence signals were detected using an Azure c600 imaging system (Azure Biosystems). ..

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    Azure Biosystems sapphire biomolecular imager
    ScFvMC1 detection and peripheral organs’ morphology. a Six-month old mice (JNPL3 shown) were IV-injected with saline, 100 μg of IRDye-labelled antibodies (scFvMC1-IR and MC1-IR) and unlabeled antibodies (scFvMC1-UNL and MC1-UNL). Two hours post-injection, brain homogenates from cortex (Ctx), hippocampus (Hip) and hindbrain (HB) were spotted on nitrocellulose and absorbance of the IRDye label measured. Saline and unlabeled antibodies showed no signal (Sapphire <t>Biomolecular</t> Imager, Azure Biosystems). b , c AAV1-CAG-scFvMC1 was injected in tibialis muscle of P301S or JNPL3 (JNPL3 shown), mice sacrificed 4 months later and peripheral organs harvested. Tissue lysates were analyzed for scFvMC1 expression with anti-Myc/tag antibody showing localized expression in the injected site ( b , right tibialis, Controls = AAV1-eGFP) while other peripheral organs did not show scFv signal ( c ). ScFvMC1: MW around 30 kDa; tubulin is used as housekeeper: MW at 55 kDa. d Hematoxylin and eosin (H E) staining was performed on kidney, liver, tibialis muscle and heart: representative images of each organs show no significant changes in morphology (Controls = AAV1-CAG-eGFP; Bright field microscope, scale bar: 300 μm)
    Sapphire Biomolecular Imager, supplied by Azure Biosystems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sapphire biomolecular imager/product/Azure Biosystems
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sapphire biomolecular imager - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    80
    Azure Biosystems sds page gel images
    Isolation of the GasK7B α antimicrobial peptide from the L. paragasseri K7 culture supernatant. (a) GasK7B α purified by reversed-phase chromatography using a 30 to 50% gradient of 2-propanol in water with 0.1% TFA. The blue line depicts the elution of protein from the column by UV detection at 280 nm. The column fractions are shown in red on the x axis. mAu, milli-absorbance units. (b) <t>SDS-PAGE</t> of GasK7B α in fractions 21 to 28 of the chromatogram shown in panel a. A prestained protein ladder (SeeBlue Plus2) was run between fractions 25 and 26 to show the relative size of the peptide.
    Sds Page Gel Images, supplied by Azure Biosystems, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sds page gel images/product/Azure Biosystems
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sds page gel images - by Bioz Stars, 2021-09
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    99
    Azure Biosystems azure c600 imaging system
    Isolation of the GasK7B α antimicrobial peptide from the L. paragasseri K7 culture supernatant. (a) GasK7B α purified by reversed-phase chromatography using a 30 to 50% gradient of 2-propanol in water with 0.1% TFA. The blue line depicts the elution of protein from the column by UV detection at 280 nm. The column fractions are shown in red on the x axis. mAu, milli-absorbance units. (b) <t>SDS-PAGE</t> of GasK7B α in fractions 21 to 28 of the chromatogram shown in panel a. A prestained protein ladder (SeeBlue Plus2) was run between fractions 25 and 26 to show the relative size of the peptide.
    Azure C600 Imaging System, supplied by Azure Biosystems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/azure c600 imaging system/product/Azure Biosystems
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    azure c600 imaging system - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    ScFvMC1 detection and peripheral organs’ morphology. a Six-month old mice (JNPL3 shown) were IV-injected with saline, 100 μg of IRDye-labelled antibodies (scFvMC1-IR and MC1-IR) and unlabeled antibodies (scFvMC1-UNL and MC1-UNL). Two hours post-injection, brain homogenates from cortex (Ctx), hippocampus (Hip) and hindbrain (HB) were spotted on nitrocellulose and absorbance of the IRDye label measured. Saline and unlabeled antibodies showed no signal (Sapphire Biomolecular Imager, Azure Biosystems). b , c AAV1-CAG-scFvMC1 was injected in tibialis muscle of P301S or JNPL3 (JNPL3 shown), mice sacrificed 4 months later and peripheral organs harvested. Tissue lysates were analyzed for scFvMC1 expression with anti-Myc/tag antibody showing localized expression in the injected site ( b , right tibialis, Controls = AAV1-eGFP) while other peripheral organs did not show scFv signal ( c ). ScFvMC1: MW around 30 kDa; tubulin is used as housekeeper: MW at 55 kDa. d Hematoxylin and eosin (H E) staining was performed on kidney, liver, tibialis muscle and heart: representative images of each organs show no significant changes in morphology (Controls = AAV1-CAG-eGFP; Bright field microscope, scale bar: 300 μm)

    Journal: Acta Neuropathologica Communications

    Article Title: Intramuscular injection of vectorized-scFvMC1 reduces pathological tau in two different tau transgenic models

    doi: 10.1186/s40478-020-01003-7

    Figure Lengend Snippet: ScFvMC1 detection and peripheral organs’ morphology. a Six-month old mice (JNPL3 shown) were IV-injected with saline, 100 μg of IRDye-labelled antibodies (scFvMC1-IR and MC1-IR) and unlabeled antibodies (scFvMC1-UNL and MC1-UNL). Two hours post-injection, brain homogenates from cortex (Ctx), hippocampus (Hip) and hindbrain (HB) were spotted on nitrocellulose and absorbance of the IRDye label measured. Saline and unlabeled antibodies showed no signal (Sapphire Biomolecular Imager, Azure Biosystems). b , c AAV1-CAG-scFvMC1 was injected in tibialis muscle of P301S or JNPL3 (JNPL3 shown), mice sacrificed 4 months later and peripheral organs harvested. Tissue lysates were analyzed for scFvMC1 expression with anti-Myc/tag antibody showing localized expression in the injected site ( b , right tibialis, Controls = AAV1-eGFP) while other peripheral organs did not show scFv signal ( c ). ScFvMC1: MW around 30 kDa; tubulin is used as housekeeper: MW at 55 kDa. d Hematoxylin and eosin (H E) staining was performed on kidney, liver, tibialis muscle and heart: representative images of each organs show no significant changes in morphology (Controls = AAV1-CAG-eGFP; Bright field microscope, scale bar: 300 μm)

    Article Snippet: Brain samples from each region were spotted on 0.45 μm nitrocellulose followed by IR signal acquisition at 789 nm, using Sapphire Biomolecular Imager (Azure Biosystems, Dublin, CA).

    Techniques: Mouse Assay, Injection, Expressing, Staining, Microscopy

    miR-155 targets FOXO3a and PI3K p85α are downregulated in EBV+ B cell lymphomas. Lysates from EBV+ and EBV− B cell lymphomas were generated with RIPA buffer supplemented with 1× Halt Protease and Phosphatase Inhibitors and 1 mM sodium orthovanadate and quantified using the Pierce 660 nm Protein Assay. About 30 μg of protein was loaded on a 4–20% tris-glycine gel and subsequently transferred to a nitrocellulose membrane. Western blots for the indicated proteins were performed as per manufacturer’s instructions and imaged via the Azure c300 digital imager. Representative blots are shown in (A) . Densitometry was performed using ImageJ, and all values were background subtracted and normalized to the indicated loading control (B–E) . Each point represents an experimental replicate. * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001 by linear mixed effects model.

    Journal: Frontiers in Microbiology

    Article Title: Epstein-Barr Virus Latent Membrane Protein 1 Regulates Host B Cell MicroRNA-155 and Its Target FOXO3a via PI3K p110α Activation

    doi: 10.3389/fmicb.2019.02692

    Figure Lengend Snippet: miR-155 targets FOXO3a and PI3K p85α are downregulated in EBV+ B cell lymphomas. Lysates from EBV+ and EBV− B cell lymphomas were generated with RIPA buffer supplemented with 1× Halt Protease and Phosphatase Inhibitors and 1 mM sodium orthovanadate and quantified using the Pierce 660 nm Protein Assay. About 30 μg of protein was loaded on a 4–20% tris-glycine gel and subsequently transferred to a nitrocellulose membrane. Western blots for the indicated proteins were performed as per manufacturer’s instructions and imaged via the Azure c300 digital imager. Representative blots are shown in (A) . Densitometry was performed using ImageJ, and all values were background subtracted and normalized to the indicated loading control (B–E) . Each point represents an experimental replicate. * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001 by linear mixed effects model.

    Article Snippet: Images were obtained on a ThermoFisher iBright FL1000 Imaging System (Colorado College) or an Azure Biosystems c300 Digital Imager (Stanford University School of Medicine).

    Techniques: Generated, Western Blot

    Isolation of the GasK7B α antimicrobial peptide from the L. paragasseri K7 culture supernatant. (a) GasK7B α purified by reversed-phase chromatography using a 30 to 50% gradient of 2-propanol in water with 0.1% TFA. The blue line depicts the elution of protein from the column by UV detection at 280 nm. The column fractions are shown in red on the x axis. mAu, milli-absorbance units. (b) SDS-PAGE of GasK7B α in fractions 21 to 28 of the chromatogram shown in panel a. A prestained protein ladder (SeeBlue Plus2) was run between fractions 25 and 26 to show the relative size of the peptide.

    Journal: Applied and Environmental Microbiology

    Article Title: Novel Selective Inhibition of Lactobacillus iners by Lactobacillus-Derived Bacteriocins

    doi: 10.1128/AEM.01594-20

    Figure Lengend Snippet: Isolation of the GasK7B α antimicrobial peptide from the L. paragasseri K7 culture supernatant. (a) GasK7B α purified by reversed-phase chromatography using a 30 to 50% gradient of 2-propanol in water with 0.1% TFA. The blue line depicts the elution of protein from the column by UV detection at 280 nm. The column fractions are shown in red on the x axis. mAu, milli-absorbance units. (b) SDS-PAGE of GasK7B α in fractions 21 to 28 of the chromatogram shown in panel a. A prestained protein ladder (SeeBlue Plus2) was run between fractions 25 and 26 to show the relative size of the peptide.

    Article Snippet: An Azure c300 digital gel analyzer was used for capturing the SDS-PAGE gel images (Azure Biosystems, CA).

    Techniques: Isolation, Purification, Reversed-phase Chromatography, SDS Page

    Analysis of purified antimicrobial peptides from L. paragasseri K7. SDS-PAGE gels show the bacteriocins purified from the L. paragasseri K7 CFS (GasK7A α [Aα], GasK7B α [Bα], and GasK7B β [Bβ]) and from E. coli Shuffle (GasK7A β [Aβ]). (Left) Bacteriocins visualized with SafeEx protein dye migrating between 3 and 6 kDa. (Right) Zones of growth inhibition of L. sakei subsp. sakei ATCC 15521 in a soft-agar overlay to detect the purified bacteriocins directly in the gel.

    Journal: Applied and Environmental Microbiology

    Article Title: Novel Selective Inhibition of Lactobacillus iners by Lactobacillus-Derived Bacteriocins

    doi: 10.1128/AEM.01594-20

    Figure Lengend Snippet: Analysis of purified antimicrobial peptides from L. paragasseri K7. SDS-PAGE gels show the bacteriocins purified from the L. paragasseri K7 CFS (GasK7A α [Aα], GasK7B α [Bα], and GasK7B β [Bβ]) and from E. coli Shuffle (GasK7A β [Aβ]). (Left) Bacteriocins visualized with SafeEx protein dye migrating between 3 and 6 kDa. (Right) Zones of growth inhibition of L. sakei subsp. sakei ATCC 15521 in a soft-agar overlay to detect the purified bacteriocins directly in the gel.

    Article Snippet: An Azure c300 digital gel analyzer was used for capturing the SDS-PAGE gel images (Azure Biosystems, CA).

    Techniques: Purification, SDS Page, Inhibition