hiscribe  (New England Biolabs)


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    Structured Review

    New England Biolabs hiscribe
    Hiscribe, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiscribe/product/New England Biolabs
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hiscribe - by Bioz Stars, 2020-04
    88/100 stars

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    Related Articles

    Gel Purification:

    Article Title: Scalable microfluidics for single-cell RNA printing and sequencing
    Article Snippet: Second, reagents from the HiScribe In Vitro Transcription kit (New England BioLabs) were substituted for the MessageAmp II kit for the IVT portion of the protocol. .. Finally, we obtained a sufficiently pure library that gel purification was unwarranted and library purification using AMpure beads was sufficient.

    RNA Sequencing Assay:

    Article Title: Scalable microfluidics for single-cell RNA printing and sequencing
    Article Snippet: Paragraph title: Procedure for single-cell RNA-Seq experiment 2 ... Second, reagents from the HiScribe In Vitro Transcription kit (New England BioLabs) were substituted for the MessageAmp II kit for the IVT portion of the protocol.

    In Vitro:

    Article Title: Scalable microfluidics for single-cell RNA printing and sequencing
    Article Snippet: .. Second, reagents from the HiScribe In Vitro Transcription kit (New England BioLabs) were substituted for the MessageAmp II kit for the IVT portion of the protocol. ..

    Purification:

    Article Title: Scalable microfluidics for single-cell RNA printing and sequencing
    Article Snippet: Second, reagents from the HiScribe In Vitro Transcription kit (New England BioLabs) were substituted for the MessageAmp II kit for the IVT portion of the protocol. .. Finally, we obtained a sufficiently pure library that gel purification was unwarranted and library purification using AMpure beads was sufficient.

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    New England Biolabs t7 quick high yield rna synthesis kit
    The effect of long ssRNA on dsDNA extension at low force (e.g. Figure 2B ) is dependent on concentration, homology, and length. Box-and-whisker plots (see Materials and Methods) show the slopes of the low force–extension curves (Figure 2B ) of the indicated samples. ( A ) <t>DNA:RNA</t> molar ratio was varied from 1:1 to 1:10 5 . Statistical analysis (asterisks) compared 1:1 versus all other ratios. ( B ) The effects of non-homologous ssRNA controls. Fluc is a 1766-nt control transcript provided in the <t>T7</t> Quick High Yield RNA Synthesis Kit, showing 43% identity with the 37 441–39 524 region of the genome of λ dsDNA. Y-RNA is the total RNA of S. cerevisiae strain. For 6KM13, bacteriophage M13mp18 circular ssDNA ∼7200 nt was amplified as the template for in vitro transcription. The resulting ssRNA is 6001 nt and presents 44% identity with the genome of λ dsDNA (region 22 893–31 255). 6L and 6H are ∼6k nt species homologous to adjacent regions of λ dsDNA (Figure 1B ). DNA:RNA molar ratio = 1:1000 in all samples; statistical analysis was performed for the ratio of λ dsDNA alone versus all other ratios. ( C ) The effects of various lengths of ssRNA (described in Figure 1B ), DNA:RNA molar ratio = 1:1000. (a, b, c) n = 10, * P
    T7 Quick High Yield Rna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/t7 quick high yield rna synthesis kit/product/New England Biolabs
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    t7 quick high yield rna synthesis kit - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    94
    New England Biolabs hiscribe t7
    The effect of long ssRNA on dsDNA extension at low force (e.g. Figure 2B ) is dependent on concentration, homology, and length. Box-and-whisker plots (see Materials and Methods) show the slopes of the low force–extension curves (Figure 2B ) of the indicated samples. ( A ) <t>DNA:RNA</t> molar ratio was varied from 1:1 to 1:10 5 . Statistical analysis (asterisks) compared 1:1 versus all other ratios. ( B ) The effects of non-homologous ssRNA controls. Fluc is a 1766-nt control transcript provided in the <t>T7</t> Quick High Yield RNA Synthesis Kit, showing 43% identity with the 37 441–39 524 region of the genome of λ dsDNA. Y-RNA is the total RNA of S. cerevisiae strain. For 6KM13, bacteriophage M13mp18 circular ssDNA ∼7200 nt was amplified as the template for in vitro transcription. The resulting ssRNA is 6001 nt and presents 44% identity with the genome of λ dsDNA (region 22 893–31 255). 6L and 6H are ∼6k nt species homologous to adjacent regions of λ dsDNA (Figure 1B ). DNA:RNA molar ratio = 1:1000 in all samples; statistical analysis was performed for the ratio of λ dsDNA alone versus all other ratios. ( C ) The effects of various lengths of ssRNA (described in Figure 1B ), DNA:RNA molar ratio = 1:1000. (a, b, c) n = 10, * P
    Hiscribe T7, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hiscribe t7/product/New England Biolabs
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    hiscribe t7 - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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    The effect of long ssRNA on dsDNA extension at low force (e.g. Figure 2B ) is dependent on concentration, homology, and length. Box-and-whisker plots (see Materials and Methods) show the slopes of the low force–extension curves (Figure 2B ) of the indicated samples. ( A ) DNA:RNA molar ratio was varied from 1:1 to 1:10 5 . Statistical analysis (asterisks) compared 1:1 versus all other ratios. ( B ) The effects of non-homologous ssRNA controls. Fluc is a 1766-nt control transcript provided in the T7 Quick High Yield RNA Synthesis Kit, showing 43% identity with the 37 441–39 524 region of the genome of λ dsDNA. Y-RNA is the total RNA of S. cerevisiae strain. For 6KM13, bacteriophage M13mp18 circular ssDNA ∼7200 nt was amplified as the template for in vitro transcription. The resulting ssRNA is 6001 nt and presents 44% identity with the genome of λ dsDNA (region 22 893–31 255). 6L and 6H are ∼6k nt species homologous to adjacent regions of λ dsDNA (Figure 1B ). DNA:RNA molar ratio = 1:1000 in all samples; statistical analysis was performed for the ratio of λ dsDNA alone versus all other ratios. ( C ) The effects of various lengths of ssRNA (described in Figure 1B ), DNA:RNA molar ratio = 1:1000. (a, b, c) n = 10, * P

    Journal: Nucleic Acids Research

    Article Title: Single molecule identification of homology-dependent interactions between long ssRNA and dsDNA

    doi: 10.1093/nar/gkw758

    Figure Lengend Snippet: The effect of long ssRNA on dsDNA extension at low force (e.g. Figure 2B ) is dependent on concentration, homology, and length. Box-and-whisker plots (see Materials and Methods) show the slopes of the low force–extension curves (Figure 2B ) of the indicated samples. ( A ) DNA:RNA molar ratio was varied from 1:1 to 1:10 5 . Statistical analysis (asterisks) compared 1:1 versus all other ratios. ( B ) The effects of non-homologous ssRNA controls. Fluc is a 1766-nt control transcript provided in the T7 Quick High Yield RNA Synthesis Kit, showing 43% identity with the 37 441–39 524 region of the genome of λ dsDNA. Y-RNA is the total RNA of S. cerevisiae strain. For 6KM13, bacteriophage M13mp18 circular ssDNA ∼7200 nt was amplified as the template for in vitro transcription. The resulting ssRNA is 6001 nt and presents 44% identity with the genome of λ dsDNA (region 22 893–31 255). 6L and 6H are ∼6k nt species homologous to adjacent regions of λ dsDNA (Figure 1B ). DNA:RNA molar ratio = 1:1000 in all samples; statistical analysis was performed for the ratio of λ dsDNA alone versus all other ratios. ( C ) The effects of various lengths of ssRNA (described in Figure 1B ), DNA:RNA molar ratio = 1:1000. (a, b, c) n = 10, * P

    Article Snippet: Fluc is a 1766-nt control transcript provided in the T7 Quick High Yield RNA Synthesis Kit, showing 43% identity with the 37 441–39 524 region of the genome of λ dsDNA.

    Techniques: Concentration Assay, Whisker Assay, Amplification, In Vitro