human aldh1a1 qpcr primer pair  (Sino Biological)


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    Name:
    Human ALDH1A1 qPCR Primer Pair
    Description:
    Verified forward and reverse primers for analyzing the quantitative expression of gene
    Catalog Number:
    hp100088
    Product Aliases:
    ALDC qPCR Primer Pairs Human, ALDH-E1 qPCR Primer Pairs Human, ALDH1 qPCR Primer Pairs Human, ALDH11 qPCR Primer Pairs Human, HEL-9 qPCR Primer Pairs Human, HEL-S-53e qPCR Primer Pairs Human, HEL12 qPCR Primer Pairs Human, PUMB1 qPCR Primer Pairs Human, RALDH1 qPCR Primer Pairs Human
    Price:
    79.0
    Size:
    1Unit
    Category:
    qPCR primer pairs
    Reactivity:
    Human
    Molecule Name:
    ALDH1A1
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    Structured Review

    Sino Biological human aldh1a1 qpcr primer pair
    Human ALDH1A1 qPCR Primer Pair
    Verified forward and reverse primers for analyzing the quantitative expression of gene
    https://www.bioz.com/result/human aldh1a1 qpcr primer pair/product/Sino Biological
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human aldh1a1 qpcr primer pair - by Bioz Stars, 2021-02
    93/100 stars

    Images

    1) Product Images from "WNT11-FZD7-DAAM1 signalling supports tumour initiating abilities and melanoma amoeboid invasion"

    Article Title: WNT11-FZD7-DAAM1 signalling supports tumour initiating abilities and melanoma amoeboid invasion

    Journal: Nature Communications

    doi: 10.1038/s41467-020-18951-2

    Analysis of the invasive front of human metastatic melanomas. a – i Representative images (left) and quantification (right) of a melanoma cell shape score, b H-score of p-MLC2 staining, c ki-67 positive cells, H-score of d DAAM1, e WNT11, f WNT5B, g ALDH1A1, h CD44 and i NANOG staining in matched TB and IF from melanoma metastases. j Model summarizing the findings of this study. The IF of human primary melanomas is enriched in cells with rounded-amoeboid morphology, high levels of Myosin II, proliferative, non-canonical Wnt and cancer stem cell-related markers. This phenotype is recapitulated and further enriched in melanoma metastasis. WNT11/5B activate FZD7 and DAAM1 to control Rho activity and then ROCK1/2-Myosin II levels. All these signalling components have an impact on amoeboid features and tumour initiation in melanoma both in vitro and in vivo. Specifically, DAAM1 plays an essential role in tumour and metastasis initiation and subsequent metastatic outgrowth by sustaining the amoeboid phenotype. a – i 45 metastatic melanomas. a , e – i Scale bar, 100 μm; inset, 50 μm. b – d Scale bar, 300 μm. a – i Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show minimum and maximum range of values. a , b , d – f Two-tailed paired t -test. c , g – i Two-tailed Wilcoxon test. Human schematic in this figure was created using Servier Medical Art templates licensed under a Creative Commons Attribution 3.0 Unported License ( https://smart.servier.com ).
    Figure Legend Snippet: Analysis of the invasive front of human metastatic melanomas. a – i Representative images (left) and quantification (right) of a melanoma cell shape score, b H-score of p-MLC2 staining, c ki-67 positive cells, H-score of d DAAM1, e WNT11, f WNT5B, g ALDH1A1, h CD44 and i NANOG staining in matched TB and IF from melanoma metastases. j Model summarizing the findings of this study. The IF of human primary melanomas is enriched in cells with rounded-amoeboid morphology, high levels of Myosin II, proliferative, non-canonical Wnt and cancer stem cell-related markers. This phenotype is recapitulated and further enriched in melanoma metastasis. WNT11/5B activate FZD7 and DAAM1 to control Rho activity and then ROCK1/2-Myosin II levels. All these signalling components have an impact on amoeboid features and tumour initiation in melanoma both in vitro and in vivo. Specifically, DAAM1 plays an essential role in tumour and metastasis initiation and subsequent metastatic outgrowth by sustaining the amoeboid phenotype. a – i 45 metastatic melanomas. a , e – i Scale bar, 100 μm; inset, 50 μm. b – d Scale bar, 300 μm. a – i Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show minimum and maximum range of values. a , b , d – f Two-tailed paired t -test. c , g – i Two-tailed Wilcoxon test. Human schematic in this figure was created using Servier Medical Art templates licensed under a Creative Commons Attribution 3.0 Unported License ( https://smart.servier.com ).

    Techniques Used: Staining, Activity Assay, In Vitro, In Vivo, Two Tailed Test

    Analysis of the invasive front of human primary melanomas. a – i Representative images (left) and quantification (right) of a melanoma cell shape score, b H-score of p-MLC2 staining, c ki-67 positive cells, H-score of d DAAM1, e WNT11, f WNT5B, g ALDH1A1, h CD44 and i NANOG staining in matched TB and IF from primary melanomas. j Principal component analysis (PCA) based on the expression of amoeboid (cell shape and p-MLC2), proliferative (ki-67), non-canonical Wnt pathway (WNT11, WNT5B and DAAM1) and cancer stem cell-related (ALDH1A1, CD44 and NANOG) markers assessed by immunohistochemistry in matched TB and IF from primary melanomas. Percentage of variation explained by each component is given in the axis labels. k , l Kaplan–Meier survival curves of k overall survival and l disease-free survival according to ALDH1A1 protein expression in the IF from our cohort of primary melanomas. ALDH1A1 expression was categorized as low or high using the median expression. a – l 53 primary melanomas. a , e – i Scale bar, 100 μm; inset, 50 μm. b – d Scale bar, 300 μm. a – i Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show the minimum and maximum range of values. a , b , d – f Two-tailed paired t -test. c , g – i Two-tailed Wilcoxon test. j Scatter plot showing principal components 1 (PC1) and 2 (PC2). k , l Log-rank test. Human schematic in this figure was created using Servier Medical Art templates licensed under a Creative Commons Attribution 3.0 Unported License ( https://smart.servier.com ).
    Figure Legend Snippet: Analysis of the invasive front of human primary melanomas. a – i Representative images (left) and quantification (right) of a melanoma cell shape score, b H-score of p-MLC2 staining, c ki-67 positive cells, H-score of d DAAM1, e WNT11, f WNT5B, g ALDH1A1, h CD44 and i NANOG staining in matched TB and IF from primary melanomas. j Principal component analysis (PCA) based on the expression of amoeboid (cell shape and p-MLC2), proliferative (ki-67), non-canonical Wnt pathway (WNT11, WNT5B and DAAM1) and cancer stem cell-related (ALDH1A1, CD44 and NANOG) markers assessed by immunohistochemistry in matched TB and IF from primary melanomas. Percentage of variation explained by each component is given in the axis labels. k , l Kaplan–Meier survival curves of k overall survival and l disease-free survival according to ALDH1A1 protein expression in the IF from our cohort of primary melanomas. ALDH1A1 expression was categorized as low or high using the median expression. a – l 53 primary melanomas. a , e – i Scale bar, 100 μm; inset, 50 μm. b – d Scale bar, 300 μm. a – i Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show the minimum and maximum range of values. a , b , d – f Two-tailed paired t -test. c , g – i Two-tailed Wilcoxon test. j Scatter plot showing principal components 1 (PC1) and 2 (PC2). k , l Log-rank test. Human schematic in this figure was created using Servier Medical Art templates licensed under a Creative Commons Attribution 3.0 Unported License ( https://smart.servier.com ).

    Techniques Used: Staining, Expressing, Immunohistochemistry, Two Tailed Test

    Non-canonical Wnt ligands support melanosphere formation and amoeboid behaviour. a mRNA expression of stem cell-related markers by qRT-PCR in A375M2 cells treated with ROCKi (GSK269962A) for 24 h compared to control A375M2 cells ( n = 4 for ALCAM , ALDH1A3 , ALDH1A1 ; n = 3 for CD44 , OCT4 , JARID1B , SOX2 ). b Schematic of treatment (top), representative phase-contrast images (bottom left) and quantification of sphere formation index (bottom right) and c cell viability of A375M2 cells and A375M2 cells treated with one dose of ROCKi (H1152 or GSK269962A) or blebbistatin ( n = 3). Scale bar, 250 μm. d – f After ROCK1/2 , MYL9 or MYL12B knockdown in A375M2 and WM1361 cells, quantification of d sphere formation index ( n = 4 for A375M2, n = 3 for WM1361) and e , f cell viability after e 7 days ( n = 3 for A375M2, n = 4 for WM1361) or f 3 days ( n = 3) of cell seeding. g – j After WNT11 or WNT5B knockdown in A375M2 and WM1361 cells, g , h representative phase-contrast images (left) and quantification of sphere formation index (right) ( n = 3) and i , j cell viability ( n = 4 for A375M2, n = 3 for WM1361). Scale bar, 250 μm. k Representative phase-contrast images (left) and quantification of cell morphology (right) of A375P cells after 24 h of WNT11 or WNT5B stimulation ( > 225 cells pooled from n = 3). Scale bar, 100 μm. a – j Graphs show mean ± s.e.m. k Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show minimum and maximum range of values. a – k n means number of independent biological experiments. a – c , h , j Two-tailed t -test. d – g , i One-way ANOVA with Dunnett post-hoc test. k Kruskal–Wallis with Dunn’s multiple comparison test. For all graphs, * p
    Figure Legend Snippet: Non-canonical Wnt ligands support melanosphere formation and amoeboid behaviour. a mRNA expression of stem cell-related markers by qRT-PCR in A375M2 cells treated with ROCKi (GSK269962A) for 24 h compared to control A375M2 cells ( n = 4 for ALCAM , ALDH1A3 , ALDH1A1 ; n = 3 for CD44 , OCT4 , JARID1B , SOX2 ). b Schematic of treatment (top), representative phase-contrast images (bottom left) and quantification of sphere formation index (bottom right) and c cell viability of A375M2 cells and A375M2 cells treated with one dose of ROCKi (H1152 or GSK269962A) or blebbistatin ( n = 3). Scale bar, 250 μm. d – f After ROCK1/2 , MYL9 or MYL12B knockdown in A375M2 and WM1361 cells, quantification of d sphere formation index ( n = 4 for A375M2, n = 3 for WM1361) and e , f cell viability after e 7 days ( n = 3 for A375M2, n = 4 for WM1361) or f 3 days ( n = 3) of cell seeding. g – j After WNT11 or WNT5B knockdown in A375M2 and WM1361 cells, g , h representative phase-contrast images (left) and quantification of sphere formation index (right) ( n = 3) and i , j cell viability ( n = 4 for A375M2, n = 3 for WM1361). Scale bar, 250 μm. k Representative phase-contrast images (left) and quantification of cell morphology (right) of A375P cells after 24 h of WNT11 or WNT5B stimulation ( > 225 cells pooled from n = 3). Scale bar, 100 μm. a – j Graphs show mean ± s.e.m. k Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show minimum and maximum range of values. a – k n means number of independent biological experiments. a – c , h , j Two-tailed t -test. d – g , i One-way ANOVA with Dunnett post-hoc test. k Kruskal–Wallis with Dunn’s multiple comparison test. For all graphs, * p

    Techniques Used: Expressing, Quantitative RT-PCR, Two Tailed Test

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: WNT11-FZD7-DAAM1 signalling supports tumour initiating abilities and melanoma amoeboid invasion
    Article Snippet: .. The following qPCR primers from Sino Biological were used: WNT11 (HP101586), WNT5B (HP101843), ALDH1A1 (HP100088), OCT4 (HP101756). .. The following QuantiTect Primers from Qiagen were used: GAPDH (QT00079247), ROCK1 (QT00034972), ROCK2 (QT00011165), MYL9 (QT00072268), MYL12B (QT00075264), FZD7 (QT00213850), RYK (QT00047292), PLCB1 (QT00038689), DAAM1 (QT00005579), ALDH1A3 (QT00077588), NANOG (QT01025850), JARID1B (QT00060648), CD44 (QT00073549), SOX2 (QT00237601), ALCAM (QT00026824).

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  • 93
    Sino Biological human aldh1a1 qpcr primer pair
    Analysis of the invasive front of human metastatic melanomas. a – i Representative images (left) and quantification (right) of a melanoma cell shape score, b H-score of p-MLC2 staining, c ki-67 positive cells, H-score of d DAAM1, e WNT11, f WNT5B, g <t>ALDH1A1,</t> h CD44 and i NANOG staining in matched TB and IF from melanoma metastases. j Model summarizing the findings of this study. The IF of human primary melanomas is enriched in cells with rounded-amoeboid morphology, high levels of Myosin II, proliferative, non-canonical Wnt and cancer stem cell-related markers. This phenotype is recapitulated and further enriched in melanoma metastasis. WNT11/5B activate FZD7 and DAAM1 to control Rho activity and then ROCK1/2-Myosin II levels. All these signalling components have an impact on amoeboid features and tumour initiation in melanoma both in vitro and in vivo. Specifically, DAAM1 plays an essential role in tumour and metastasis initiation and subsequent metastatic outgrowth by sustaining the amoeboid phenotype. a – i 45 metastatic melanomas. a , e – i Scale bar, 100 μm; inset, 50 μm. b – d Scale bar, 300 μm. a – i Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show minimum and maximum range of values. a , b , d – f Two-tailed paired t -test. c , g – i Two-tailed Wilcoxon test. Human schematic in this figure was created using Servier Medical Art templates licensed under a Creative Commons Attribution 3.0 Unported License ( https://smart.servier.com ).
    Human Aldh1a1 Qpcr Primer Pair, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human aldh1a1 qpcr primer pair/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human aldh1a1 qpcr primer pair - by Bioz Stars, 2021-02
    93/100 stars
      Buy from Supplier

    93
    Sino Biological human gapdh qpcr primer pair
    Analysis of the invasive front of human metastatic melanomas. a – i Representative images (left) and quantification (right) of a melanoma cell shape score, b H-score of p-MLC2 staining, c ki-67 positive cells, H-score of d DAAM1, e WNT11, f WNT5B, g <t>ALDH1A1,</t> h CD44 and i NANOG staining in matched TB and IF from melanoma metastases. j Model summarizing the findings of this study. The IF of human primary melanomas is enriched in cells with rounded-amoeboid morphology, high levels of Myosin II, proliferative, non-canonical Wnt and cancer stem cell-related markers. This phenotype is recapitulated and further enriched in melanoma metastasis. WNT11/5B activate FZD7 and DAAM1 to control Rho activity and then ROCK1/2-Myosin II levels. All these signalling components have an impact on amoeboid features and tumour initiation in melanoma both in vitro and in vivo. Specifically, DAAM1 plays an essential role in tumour and metastasis initiation and subsequent metastatic outgrowth by sustaining the amoeboid phenotype. a – i 45 metastatic melanomas. a , e – i Scale bar, 100 μm; inset, 50 μm. b – d Scale bar, 300 μm. a – i Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show minimum and maximum range of values. a , b , d – f Two-tailed paired t -test. c , g – i Two-tailed Wilcoxon test. Human schematic in this figure was created using Servier Medical Art templates licensed under a Creative Commons Attribution 3.0 Unported License ( https://smart.servier.com ).
    Human Gapdh Qpcr Primer Pair, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human gapdh qpcr primer pair/product/Sino Biological
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    human gapdh qpcr primer pair - by Bioz Stars, 2021-02
    93/100 stars
      Buy from Supplier

    90
    peprotech human gapdh qpcr primer pair
    Analysis of the invasive front of human metastatic melanomas. a – i Representative images (left) and quantification (right) of a melanoma cell shape score, b H-score of p-MLC2 staining, c ki-67 positive cells, H-score of d DAAM1, e WNT11, f WNT5B, g <t>ALDH1A1,</t> h CD44 and i NANOG staining in matched TB and IF from melanoma metastases. j Model summarizing the findings of this study. The IF of human primary melanomas is enriched in cells with rounded-amoeboid morphology, high levels of Myosin II, proliferative, non-canonical Wnt and cancer stem cell-related markers. This phenotype is recapitulated and further enriched in melanoma metastasis. WNT11/5B activate FZD7 and DAAM1 to control Rho activity and then ROCK1/2-Myosin II levels. All these signalling components have an impact on amoeboid features and tumour initiation in melanoma both in vitro and in vivo. Specifically, DAAM1 plays an essential role in tumour and metastasis initiation and subsequent metastatic outgrowth by sustaining the amoeboid phenotype. a – i 45 metastatic melanomas. a , e – i Scale bar, 100 μm; inset, 50 μm. b – d Scale bar, 300 μm. a – i Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show minimum and maximum range of values. a , b , d – f Two-tailed paired t -test. c , g – i Two-tailed Wilcoxon test. Human schematic in this figure was created using Servier Medical Art templates licensed under a Creative Commons Attribution 3.0 Unported License ( https://smart.servier.com ).
    Human Gapdh Qpcr Primer Pair, supplied by peprotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human gapdh qpcr primer pair/product/peprotech
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human gapdh qpcr primer pair - by Bioz Stars, 2021-02
    90/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of the invasive front of human metastatic melanomas. a – i Representative images (left) and quantification (right) of a melanoma cell shape score, b H-score of p-MLC2 staining, c ki-67 positive cells, H-score of d DAAM1, e WNT11, f WNT5B, g ALDH1A1, h CD44 and i NANOG staining in matched TB and IF from melanoma metastases. j Model summarizing the findings of this study. The IF of human primary melanomas is enriched in cells with rounded-amoeboid morphology, high levels of Myosin II, proliferative, non-canonical Wnt and cancer stem cell-related markers. This phenotype is recapitulated and further enriched in melanoma metastasis. WNT11/5B activate FZD7 and DAAM1 to control Rho activity and then ROCK1/2-Myosin II levels. All these signalling components have an impact on amoeboid features and tumour initiation in melanoma both in vitro and in vivo. Specifically, DAAM1 plays an essential role in tumour and metastasis initiation and subsequent metastatic outgrowth by sustaining the amoeboid phenotype. a – i 45 metastatic melanomas. a , e – i Scale bar, 100 μm; inset, 50 μm. b – d Scale bar, 300 μm. a – i Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show minimum and maximum range of values. a , b , d – f Two-tailed paired t -test. c , g – i Two-tailed Wilcoxon test. Human schematic in this figure was created using Servier Medical Art templates licensed under a Creative Commons Attribution 3.0 Unported License ( https://smart.servier.com ).

    Journal: Nature Communications

    Article Title: WNT11-FZD7-DAAM1 signalling supports tumour initiating abilities and melanoma amoeboid invasion

    doi: 10.1038/s41467-020-18951-2

    Figure Lengend Snippet: Analysis of the invasive front of human metastatic melanomas. a – i Representative images (left) and quantification (right) of a melanoma cell shape score, b H-score of p-MLC2 staining, c ki-67 positive cells, H-score of d DAAM1, e WNT11, f WNT5B, g ALDH1A1, h CD44 and i NANOG staining in matched TB and IF from melanoma metastases. j Model summarizing the findings of this study. The IF of human primary melanomas is enriched in cells with rounded-amoeboid morphology, high levels of Myosin II, proliferative, non-canonical Wnt and cancer stem cell-related markers. This phenotype is recapitulated and further enriched in melanoma metastasis. WNT11/5B activate FZD7 and DAAM1 to control Rho activity and then ROCK1/2-Myosin II levels. All these signalling components have an impact on amoeboid features and tumour initiation in melanoma both in vitro and in vivo. Specifically, DAAM1 plays an essential role in tumour and metastasis initiation and subsequent metastatic outgrowth by sustaining the amoeboid phenotype. a – i 45 metastatic melanomas. a , e – i Scale bar, 100 μm; inset, 50 μm. b – d Scale bar, 300 μm. a – i Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show minimum and maximum range of values. a , b , d – f Two-tailed paired t -test. c , g – i Two-tailed Wilcoxon test. Human schematic in this figure was created using Servier Medical Art templates licensed under a Creative Commons Attribution 3.0 Unported License ( https://smart.servier.com ).

    Article Snippet: The following qPCR primers from Sino Biological were used: WNT11 (HP101586), WNT5B (HP101843), ALDH1A1 (HP100088), OCT4 (HP101756).

    Techniques: Staining, Activity Assay, In Vitro, In Vivo, Two Tailed Test

    Analysis of the invasive front of human primary melanomas. a – i Representative images (left) and quantification (right) of a melanoma cell shape score, b H-score of p-MLC2 staining, c ki-67 positive cells, H-score of d DAAM1, e WNT11, f WNT5B, g ALDH1A1, h CD44 and i NANOG staining in matched TB and IF from primary melanomas. j Principal component analysis (PCA) based on the expression of amoeboid (cell shape and p-MLC2), proliferative (ki-67), non-canonical Wnt pathway (WNT11, WNT5B and DAAM1) and cancer stem cell-related (ALDH1A1, CD44 and NANOG) markers assessed by immunohistochemistry in matched TB and IF from primary melanomas. Percentage of variation explained by each component is given in the axis labels. k , l Kaplan–Meier survival curves of k overall survival and l disease-free survival according to ALDH1A1 protein expression in the IF from our cohort of primary melanomas. ALDH1A1 expression was categorized as low or high using the median expression. a – l 53 primary melanomas. a , e – i Scale bar, 100 μm; inset, 50 μm. b – d Scale bar, 300 μm. a – i Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show the minimum and maximum range of values. a , b , d – f Two-tailed paired t -test. c , g – i Two-tailed Wilcoxon test. j Scatter plot showing principal components 1 (PC1) and 2 (PC2). k , l Log-rank test. Human schematic in this figure was created using Servier Medical Art templates licensed under a Creative Commons Attribution 3.0 Unported License ( https://smart.servier.com ).

    Journal: Nature Communications

    Article Title: WNT11-FZD7-DAAM1 signalling supports tumour initiating abilities and melanoma amoeboid invasion

    doi: 10.1038/s41467-020-18951-2

    Figure Lengend Snippet: Analysis of the invasive front of human primary melanomas. a – i Representative images (left) and quantification (right) of a melanoma cell shape score, b H-score of p-MLC2 staining, c ki-67 positive cells, H-score of d DAAM1, e WNT11, f WNT5B, g ALDH1A1, h CD44 and i NANOG staining in matched TB and IF from primary melanomas. j Principal component analysis (PCA) based on the expression of amoeboid (cell shape and p-MLC2), proliferative (ki-67), non-canonical Wnt pathway (WNT11, WNT5B and DAAM1) and cancer stem cell-related (ALDH1A1, CD44 and NANOG) markers assessed by immunohistochemistry in matched TB and IF from primary melanomas. Percentage of variation explained by each component is given in the axis labels. k , l Kaplan–Meier survival curves of k overall survival and l disease-free survival according to ALDH1A1 protein expression in the IF from our cohort of primary melanomas. ALDH1A1 expression was categorized as low or high using the median expression. a – l 53 primary melanomas. a , e – i Scale bar, 100 μm; inset, 50 μm. b – d Scale bar, 300 μm. a – i Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show the minimum and maximum range of values. a , b , d – f Two-tailed paired t -test. c , g – i Two-tailed Wilcoxon test. j Scatter plot showing principal components 1 (PC1) and 2 (PC2). k , l Log-rank test. Human schematic in this figure was created using Servier Medical Art templates licensed under a Creative Commons Attribution 3.0 Unported License ( https://smart.servier.com ).

    Article Snippet: The following qPCR primers from Sino Biological were used: WNT11 (HP101586), WNT5B (HP101843), ALDH1A1 (HP100088), OCT4 (HP101756).

    Techniques: Staining, Expressing, Immunohistochemistry, Two Tailed Test

    Non-canonical Wnt ligands support melanosphere formation and amoeboid behaviour. a mRNA expression of stem cell-related markers by qRT-PCR in A375M2 cells treated with ROCKi (GSK269962A) for 24 h compared to control A375M2 cells ( n = 4 for ALCAM , ALDH1A3 , ALDH1A1 ; n = 3 for CD44 , OCT4 , JARID1B , SOX2 ). b Schematic of treatment (top), representative phase-contrast images (bottom left) and quantification of sphere formation index (bottom right) and c cell viability of A375M2 cells and A375M2 cells treated with one dose of ROCKi (H1152 or GSK269962A) or blebbistatin ( n = 3). Scale bar, 250 μm. d – f After ROCK1/2 , MYL9 or MYL12B knockdown in A375M2 and WM1361 cells, quantification of d sphere formation index ( n = 4 for A375M2, n = 3 for WM1361) and e , f cell viability after e 7 days ( n = 3 for A375M2, n = 4 for WM1361) or f 3 days ( n = 3) of cell seeding. g – j After WNT11 or WNT5B knockdown in A375M2 and WM1361 cells, g , h representative phase-contrast images (left) and quantification of sphere formation index (right) ( n = 3) and i , j cell viability ( n = 4 for A375M2, n = 3 for WM1361). Scale bar, 250 μm. k Representative phase-contrast images (left) and quantification of cell morphology (right) of A375P cells after 24 h of WNT11 or WNT5B stimulation ( > 225 cells pooled from n = 3). Scale bar, 100 μm. a – j Graphs show mean ± s.e.m. k Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show minimum and maximum range of values. a – k n means number of independent biological experiments. a – c , h , j Two-tailed t -test. d – g , i One-way ANOVA with Dunnett post-hoc test. k Kruskal–Wallis with Dunn’s multiple comparison test. For all graphs, * p

    Journal: Nature Communications

    Article Title: WNT11-FZD7-DAAM1 signalling supports tumour initiating abilities and melanoma amoeboid invasion

    doi: 10.1038/s41467-020-18951-2

    Figure Lengend Snippet: Non-canonical Wnt ligands support melanosphere formation and amoeboid behaviour. a mRNA expression of stem cell-related markers by qRT-PCR in A375M2 cells treated with ROCKi (GSK269962A) for 24 h compared to control A375M2 cells ( n = 4 for ALCAM , ALDH1A3 , ALDH1A1 ; n = 3 for CD44 , OCT4 , JARID1B , SOX2 ). b Schematic of treatment (top), representative phase-contrast images (bottom left) and quantification of sphere formation index (bottom right) and c cell viability of A375M2 cells and A375M2 cells treated with one dose of ROCKi (H1152 or GSK269962A) or blebbistatin ( n = 3). Scale bar, 250 μm. d – f After ROCK1/2 , MYL9 or MYL12B knockdown in A375M2 and WM1361 cells, quantification of d sphere formation index ( n = 4 for A375M2, n = 3 for WM1361) and e , f cell viability after e 7 days ( n = 3 for A375M2, n = 4 for WM1361) or f 3 days ( n = 3) of cell seeding. g – j After WNT11 or WNT5B knockdown in A375M2 and WM1361 cells, g , h representative phase-contrast images (left) and quantification of sphere formation index (right) ( n = 3) and i , j cell viability ( n = 4 for A375M2, n = 3 for WM1361). Scale bar, 250 μm. k Representative phase-contrast images (left) and quantification of cell morphology (right) of A375P cells after 24 h of WNT11 or WNT5B stimulation ( > 225 cells pooled from n = 3). Scale bar, 100 μm. a – j Graphs show mean ± s.e.m. k Box limits show 25th and 75th percentiles, the horizontal line shows the median, and whiskers show minimum and maximum range of values. a – k n means number of independent biological experiments. a – c , h , j Two-tailed t -test. d – g , i One-way ANOVA with Dunnett post-hoc test. k Kruskal–Wallis with Dunn’s multiple comparison test. For all graphs, * p

    Article Snippet: The following qPCR primers from Sino Biological were used: WNT11 (HP101586), WNT5B (HP101843), ALDH1A1 (HP100088), OCT4 (HP101756).

    Techniques: Expressing, Quantitative RT-PCR, Two Tailed Test