multiplex pcr protocol  (Solis BioDyne)


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    Name:
    HOT FIREPol MultiPlex Mix Ready To Load
    Description:
    Hot start master mix for high throughput applications with ready to load feature Analyze multiple targets per reaction Increased sensitivity and yield Prevents primer dimer formation Reaction set up and shipment without dry ice
    Catalog Number:
    04-36-00120
    Price:
    None
    Category:
    Endpoint PCR
    Applications:
    PCR, HOT-start, multiplex PCR, direct loading to gel
    Size:
    1 ml | 250 rxn
    Buy from Supplier


    Structured Review

    Solis BioDyne multiplex pcr protocol
    HOT FIREPol MultiPlex Mix Ready To Load
    Hot start master mix for high throughput applications with ready to load feature Analyze multiple targets per reaction Increased sensitivity and yield Prevents primer dimer formation Reaction set up and shipment without dry ice
    https://www.bioz.com/result/multiplex pcr protocol/product/Solis BioDyne
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multiplex pcr protocol - by Bioz Stars, 2021-09
    93/100 stars

    Images

    1) Product Images from "Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso"

    Article Title: Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0232115

    Detection of the five bacterial taxa using the newly described multiplex PCR protocol. L: molecular size marker, 100 bp DNA ladder ready to load, Solis Biodyne, Pfs : P . fuscovaginae strain UBP735, Bg : B . glumae strain NCPPB 3923, Sph : Sphingomonas strain V1-2, Xoc : X . oryzae pv. oryzicola strain BAI10, Pan : Pantoea strain ARC10, Mix: Equal amounts of all five DNA samples.
    Figure Legend Snippet: Detection of the five bacterial taxa using the newly described multiplex PCR protocol. L: molecular size marker, 100 bp DNA ladder ready to load, Solis Biodyne, Pfs : P . fuscovaginae strain UBP735, Bg : B . glumae strain NCPPB 3923, Sph : Sphingomonas strain V1-2, Xoc : X . oryzae pv. oryzicola strain BAI10, Pan : Pantoea strain ARC10, Mix: Equal amounts of all five DNA samples.

    Techniques Used: Multiplex Assay, Polymerase Chain Reaction, Marker

    Related Articles

    Multiplex Assay:

    Article Title: Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso
    Article Snippet: .. Multiplex PCR assay optimization Following the optimization, the final conditions for the multiplex PCR protocol were: 2 μl of DNA added to 23 μl master mix comprising 5 μl Hot Firepol Multiplex Mix ready to load 5X (Solis BioDyne,Tartu, Estonia), 1.25 μl (NH4 )2 SO4 (160 mM), 0.2 μl of each primers specific of P . fuscovaginae at 5 μM, 0.2 μl of each primers specific of B . gluma e and B . gladioli at 100 μM, 0.3 μl of each primers specific of Pantoea spp. at 100 μM and 0.3 μl of each primers specific of X . oryzae at 10 μM and finally 0.1 μl of each primers specific of Sphingomonas spp. at 10 μM. ..

    Polymerase Chain Reaction:

    Article Title: Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso
    Article Snippet: .. Multiplex PCR assay optimization Following the optimization, the final conditions for the multiplex PCR protocol were: 2 μl of DNA added to 23 μl master mix comprising 5 μl Hot Firepol Multiplex Mix ready to load 5X (Solis BioDyne,Tartu, Estonia), 1.25 μl (NH4 )2 SO4 (160 mM), 0.2 μl of each primers specific of P . fuscovaginae at 5 μM, 0.2 μl of each primers specific of B . gluma e and B . gladioli at 100 μM, 0.3 μl of each primers specific of Pantoea spp. at 100 μM and 0.3 μl of each primers specific of X . oryzae at 10 μM and finally 0.1 μl of each primers specific of Sphingomonas spp. at 10 μM. ..

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  • 93
    Solis BioDyne multiplex pcr protocol
    Detection of the five bacterial taxa using the newly described multiplex <t>PCR</t> protocol. L: molecular size marker, 100 bp <t>DNA</t> ladder ready to load, Solis Biodyne, Pfs : P . fuscovaginae strain UBP735, Bg : B . glumae strain NCPPB 3923, Sph : Sphingomonas strain V1-2, Xoc : X . oryzae pv. oryzicola strain BAI10, Pan : Pantoea strain ARC10, Mix: Equal amounts of all five DNA samples.
    Multiplex Pcr Protocol, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/multiplex pcr protocol/product/Solis BioDyne
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    multiplex pcr protocol - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

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    Detection of the five bacterial taxa using the newly described multiplex PCR protocol. L: molecular size marker, 100 bp DNA ladder ready to load, Solis Biodyne, Pfs : P . fuscovaginae strain UBP735, Bg : B . glumae strain NCPPB 3923, Sph : Sphingomonas strain V1-2, Xoc : X . oryzae pv. oryzicola strain BAI10, Pan : Pantoea strain ARC10, Mix: Equal amounts of all five DNA samples.

    Journal: PLoS ONE

    Article Title: Design of a new multiplex PCR assay for rice pathogenic bacteria detection and its application to infer disease incidence and detect co-infection in rice fields in Burkina Faso

    doi: 10.1371/journal.pone.0232115

    Figure Lengend Snippet: Detection of the five bacterial taxa using the newly described multiplex PCR protocol. L: molecular size marker, 100 bp DNA ladder ready to load, Solis Biodyne, Pfs : P . fuscovaginae strain UBP735, Bg : B . glumae strain NCPPB 3923, Sph : Sphingomonas strain V1-2, Xoc : X . oryzae pv. oryzicola strain BAI10, Pan : Pantoea strain ARC10, Mix: Equal amounts of all five DNA samples.

    Article Snippet: Multiplex PCR assay optimization Following the optimization, the final conditions for the multiplex PCR protocol were: 2 μl of DNA added to 23 μl master mix comprising 5 μl Hot Firepol Multiplex Mix ready to load 5X (Solis BioDyne,Tartu, Estonia), 1.25 μl (NH4 )2 SO4 (160 mM), 0.2 μl of each primers specific of P . fuscovaginae at 5 μM, 0.2 μl of each primers specific of B . gluma e and B . gladioli at 100 μM, 0.3 μl of each primers specific of Pantoea spp. at 100 μM and 0.3 μl of each primers specific of X . oryzae at 10 μM and finally 0.1 μl of each primers specific of Sphingomonas spp. at 10 μM.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Marker