ppp2r4 cdna orf clone human untagged  (Sino Biological)


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    • 92
    Name:
    Ppp2r4 cDNA ORF Clone Human untagged
    Description:
    Full length Clone DNA of Human protein phosphatase 2A activator regulatory subunit 4
    Catalog Number:
    hg12287-ut
    Product Aliases:
    PP2A cDNA ORF Clone Human, PR53 cDNA ORF Clone Human, PTPA cDNA ORF Clone Human
    Price:
    195.0
    Applications:
    Stable or Transient mammalian expression
    Size:
    1Unit
    Category:
    cDNA Clone
    Molecule Name:
    PTPA,PR53,Ppp2r4,
    		Buy from Supplier

    Structured Review

    Sino Biological ppp2r4 cdna orf clone human untagged
    Ppp2r4 cDNA ORF Clone Human untagged
    Full length Clone DNA of Human protein phosphatase 2A activator regulatory subunit 4
    https://www.bioz.com/result/ppp2r4 cdna orf clone human untagged/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ppp2r4 cdna orf clone human untagged - by Bioz Stars, 2021-02
    92/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. Similarly, pAAV-EF1a control vector with 50 bp insert was created by annealing complementary oligonucleotides with 15 bp overhangs (10x annealing buffer: 100 nM Tris HCl, 500 mM NaCl, 10 mM EDTA) and recombined with In-Fusion HD cloning kit into the pAAV-EF1a-PREP backbone. pCMV3-PPP2AC-C-FLAG (HG10420-CF), pCMV3-N-FLAG-PPP2AC (HG10420NF), pCMV3-PPP2R4A (HG12287-UT), and pCMV3-N-FLAG-PPP2R4A (HG12287NF) were purchased from Sino-Biological. pAAV-EF1a-FLAG(C)-PME1 and pAAVEF1a-PME1-FLAG(N) inserts were amplified from pUC19-PPME1 (HG29687-U, SinoJo ur na l re -p ro of Biological) with overhangs containing either N- or C-terminal FLAG sequence. pAAV1EF1α-PREP backbone was digested with KpnI-HF (NEB) and EcoRV-HF (NEB), gel purified and recombined with all of the aforementioned inserts using an In-Fusion HD cloning kit (Clontech). .. For protein-fragment complementation assay (PCA), cDNA of PREP, all of the PREP mutants, PP2Ac, PME1 and PTPA were cloned in a pcDNA3.1/zeo backbone containing humanized G. princeps PCA fragments (GLuc) [27].

    Amplification:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. PREP mutants (D149A-PREP, T202C-PREP, T204A-PREP, T590C-PREP, H680APREP and LoopA-PREP), PP2Ac (HG10420-CF, Sino-Biological), PME1 (SinoBiological) and PTPA (HG12287-UT, Sino-Biological) cDNA was PCR amplified to create insert oligonucleotides with 15 bp overhangs. .. Inserts were gel purified and recombined with EcoRI-HF (NEB) and KpnI-HF (NEB) digested and gel purified GLuc1N, Gluc-2N, GLuc-1C, and GLuc-2C backbone using In-Fusion cloning kit (Clontech).

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. Similarly, pAAV-EF1a control vector with 50 bp insert was created by annealing complementary oligonucleotides with 15 bp overhangs (10x annealing buffer: 100 nM Tris HCl, 500 mM NaCl, 10 mM EDTA) and recombined with In-Fusion HD cloning kit into the pAAV-EF1a-PREP backbone. pCMV3-PPP2AC-C-FLAG (HG10420-CF), pCMV3-N-FLAG-PPP2AC (HG10420NF), pCMV3-PPP2R4A (HG12287-UT), and pCMV3-N-FLAG-PPP2R4A (HG12287NF) were purchased from Sino-Biological. pAAV-EF1a-FLAG(C)-PME1 and pAAVEF1a-PME1-FLAG(N) inserts were amplified from pUC19-PPME1 (HG29687-U, SinoJo ur na l re -p ro of Biological) with overhangs containing either N- or C-terminal FLAG sequence. pAAV1EF1α-PREP backbone was digested with KpnI-HF (NEB) and EcoRV-HF (NEB), gel purified and recombined with all of the aforementioned inserts using an In-Fusion HD cloning kit (Clontech). .. For protein-fragment complementation assay (PCA), cDNA of PREP, all of the PREP mutants, PP2Ac, PME1 and PTPA were cloned in a pcDNA3.1/zeo backbone containing humanized G. princeps PCA fragments (GLuc) [27].

    Purification:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. Similarly, pAAV-EF1a control vector with 50 bp insert was created by annealing complementary oligonucleotides with 15 bp overhangs (10x annealing buffer: 100 nM Tris HCl, 500 mM NaCl, 10 mM EDTA) and recombined with In-Fusion HD cloning kit into the pAAV-EF1a-PREP backbone. pCMV3-PPP2AC-C-FLAG (HG10420-CF), pCMV3-N-FLAG-PPP2AC (HG10420NF), pCMV3-PPP2R4A (HG12287-UT), and pCMV3-N-FLAG-PPP2R4A (HG12287NF) were purchased from Sino-Biological. pAAV-EF1a-FLAG(C)-PME1 and pAAVEF1a-PME1-FLAG(N) inserts were amplified from pUC19-PPME1 (HG29687-U, SinoJo ur na l re -p ro of Biological) with overhangs containing either N- or C-terminal FLAG sequence. pAAV1EF1α-PREP backbone was digested with KpnI-HF (NEB) and EcoRV-HF (NEB), gel purified and recombined with all of the aforementioned inserts using an In-Fusion HD cloning kit (Clontech). .. For protein-fragment complementation assay (PCA), cDNA of PREP, all of the PREP mutants, PP2Ac, PME1 and PTPA were cloned in a pcDNA3.1/zeo backbone containing humanized G. princeps PCA fragments (GLuc) [27].

    Polymerase Chain Reaction:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. PREP mutants (D149A-PREP, T202C-PREP, T204A-PREP, T590C-PREP, H680APREP and LoopA-PREP), PP2Ac (HG10420-CF, Sino-Biological), PME1 (SinoBiological) and PTPA (HG12287-UT, Sino-Biological) cDNA was PCR amplified to create insert oligonucleotides with 15 bp overhangs. .. Inserts were gel purified and recombined with EcoRI-HF (NEB) and KpnI-HF (NEB) digested and gel purified GLuc1N, Gluc-2N, GLuc-1C, and GLuc-2C backbone using In-Fusion cloning kit (Clontech).

    Sequencing:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. Similarly, pAAV-EF1a control vector with 50 bp insert was created by annealing complementary oligonucleotides with 15 bp overhangs (10x annealing buffer: 100 nM Tris HCl, 500 mM NaCl, 10 mM EDTA) and recombined with In-Fusion HD cloning kit into the pAAV-EF1a-PREP backbone. pCMV3-PPP2AC-C-FLAG (HG10420-CF), pCMV3-N-FLAG-PPP2AC (HG10420NF), pCMV3-PPP2R4A (HG12287-UT), and pCMV3-N-FLAG-PPP2R4A (HG12287NF) were purchased from Sino-Biological. pAAV-EF1a-FLAG(C)-PME1 and pAAVEF1a-PME1-FLAG(N) inserts were amplified from pUC19-PPME1 (HG29687-U, SinoJo ur na l re -p ro of Biological) with overhangs containing either N- or C-terminal FLAG sequence. pAAV1EF1α-PREP backbone was digested with KpnI-HF (NEB) and EcoRV-HF (NEB), gel purified and recombined with all of the aforementioned inserts using an In-Fusion HD cloning kit (Clontech). .. For protein-fragment complementation assay (PCA), cDNA of PREP, all of the PREP mutants, PP2Ac, PME1 and PTPA were cloned in a pcDNA3.1/zeo backbone containing humanized G. princeps PCA fragments (GLuc) [27].

    Plasmid Preparation:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. Similarly, pAAV-EF1a control vector with 50 bp insert was created by annealing complementary oligonucleotides with 15 bp overhangs (10x annealing buffer: 100 nM Tris HCl, 500 mM NaCl, 10 mM EDTA) and recombined with In-Fusion HD cloning kit into the pAAV-EF1a-PREP backbone. pCMV3-PPP2AC-C-FLAG (HG10420-CF), pCMV3-N-FLAG-PPP2AC (HG10420NF), pCMV3-PPP2R4A (HG12287-UT), and pCMV3-N-FLAG-PPP2R4A (HG12287NF) were purchased from Sino-Biological. pAAV-EF1a-FLAG(C)-PME1 and pAAVEF1a-PME1-FLAG(N) inserts were amplified from pUC19-PPME1 (HG29687-U, SinoJo ur na l re -p ro of Biological) with overhangs containing either N- or C-terminal FLAG sequence. pAAV1EF1α-PREP backbone was digested with KpnI-HF (NEB) and EcoRV-HF (NEB), gel purified and recombined with all of the aforementioned inserts using an In-Fusion HD cloning kit (Clontech). .. For protein-fragment complementation assay (PCA), cDNA of PREP, all of the PREP mutants, PP2Ac, PME1 and PTPA were cloned in a pcDNA3.1/zeo backbone containing humanized G. princeps PCA fragments (GLuc) [27].

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