ppp2r4 cdna orf clone human untagged  (Sino Biological)


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    Name:
    Ppp2r4 cDNA ORF Clone Human untagged
    Description:
    Full length Clone DNA of Human protein phosphatase 2A activator regulatory subunit 4
    Catalog Number:
    hg12287-ut
    Product Aliases:
    PP2A cDNA ORF Clone Human, PR53 cDNA ORF Clone Human, PTPA cDNA ORF Clone Human
    Price:
    195.0
    Applications:
    Stable or Transient mammalian expression
    Size:
    1Unit
    Category:
    cDNA Clone
    Molecule Name:
    PTPA,PR53,Ppp2r4,
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    Structured Review

    Sino Biological ppp2r4 cdna orf clone human untagged
    Ppp2r4 cDNA ORF Clone Human untagged
    Full length Clone DNA of Human protein phosphatase 2A activator regulatory subunit 4
    https://www.bioz.com/result/ppp2r4 cdna orf clone human untagged/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ppp2r4 cdna orf clone human untagged - by Bioz Stars, 2021-02
    92/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. Similarly, pAAV-EF1a control vector with 50 bp insert was created by annealing complementary oligonucleotides with 15 bp overhangs (10x annealing buffer: 100 nM Tris HCl, 500 mM NaCl, 10 mM EDTA) and recombined with In-Fusion HD cloning kit into the pAAV-EF1a-PREP backbone. pCMV3-PPP2AC-C-FLAG (HG10420-CF), pCMV3-N-FLAG-PPP2AC (HG10420NF), pCMV3-PPP2R4A (HG12287-UT), and pCMV3-N-FLAG-PPP2R4A (HG12287NF) were purchased from Sino-Biological. pAAV-EF1a-FLAG(C)-PME1 and pAAVEF1a-PME1-FLAG(N) inserts were amplified from pUC19-PPME1 (HG29687-U, SinoJo ur na l re -p ro of Biological) with overhangs containing either N- or C-terminal FLAG sequence. pAAV1EF1α-PREP backbone was digested with KpnI-HF (NEB) and EcoRV-HF (NEB), gel purified and recombined with all of the aforementioned inserts using an In-Fusion HD cloning kit (Clontech). .. For protein-fragment complementation assay (PCA), cDNA of PREP, all of the PREP mutants, PP2Ac, PME1 and PTPA were cloned in a pcDNA3.1/zeo backbone containing humanized G. princeps PCA fragments (GLuc) [27].

    Amplification:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. PREP mutants (D149A-PREP, T202C-PREP, T204A-PREP, T590C-PREP, H680APREP and LoopA-PREP), PP2Ac (HG10420-CF, Sino-Biological), PME1 (SinoBiological) and PTPA (HG12287-UT, Sino-Biological) cDNA was PCR amplified to create insert oligonucleotides with 15 bp overhangs. .. Inserts were gel purified and recombined with EcoRI-HF (NEB) and KpnI-HF (NEB) digested and gel purified GLuc1N, Gluc-2N, GLuc-1C, and GLuc-2C backbone using In-Fusion cloning kit (Clontech).

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. Similarly, pAAV-EF1a control vector with 50 bp insert was created by annealing complementary oligonucleotides with 15 bp overhangs (10x annealing buffer: 100 nM Tris HCl, 500 mM NaCl, 10 mM EDTA) and recombined with In-Fusion HD cloning kit into the pAAV-EF1a-PREP backbone. pCMV3-PPP2AC-C-FLAG (HG10420-CF), pCMV3-N-FLAG-PPP2AC (HG10420NF), pCMV3-PPP2R4A (HG12287-UT), and pCMV3-N-FLAG-PPP2R4A (HG12287NF) were purchased from Sino-Biological. pAAV-EF1a-FLAG(C)-PME1 and pAAVEF1a-PME1-FLAG(N) inserts were amplified from pUC19-PPME1 (HG29687-U, SinoJo ur na l re -p ro of Biological) with overhangs containing either N- or C-terminal FLAG sequence. pAAV1EF1α-PREP backbone was digested with KpnI-HF (NEB) and EcoRV-HF (NEB), gel purified and recombined with all of the aforementioned inserts using an In-Fusion HD cloning kit (Clontech). .. For protein-fragment complementation assay (PCA), cDNA of PREP, all of the PREP mutants, PP2Ac, PME1 and PTPA were cloned in a pcDNA3.1/zeo backbone containing humanized G. princeps PCA fragments (GLuc) [27].

    Purification:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. Similarly, pAAV-EF1a control vector with 50 bp insert was created by annealing complementary oligonucleotides with 15 bp overhangs (10x annealing buffer: 100 nM Tris HCl, 500 mM NaCl, 10 mM EDTA) and recombined with In-Fusion HD cloning kit into the pAAV-EF1a-PREP backbone. pCMV3-PPP2AC-C-FLAG (HG10420-CF), pCMV3-N-FLAG-PPP2AC (HG10420NF), pCMV3-PPP2R4A (HG12287-UT), and pCMV3-N-FLAG-PPP2R4A (HG12287NF) were purchased from Sino-Biological. pAAV-EF1a-FLAG(C)-PME1 and pAAVEF1a-PME1-FLAG(N) inserts were amplified from pUC19-PPME1 (HG29687-U, SinoJo ur na l re -p ro of Biological) with overhangs containing either N- or C-terminal FLAG sequence. pAAV1EF1α-PREP backbone was digested with KpnI-HF (NEB) and EcoRV-HF (NEB), gel purified and recombined with all of the aforementioned inserts using an In-Fusion HD cloning kit (Clontech). .. For protein-fragment complementation assay (PCA), cDNA of PREP, all of the PREP mutants, PP2Ac, PME1 and PTPA were cloned in a pcDNA3.1/zeo backbone containing humanized G. princeps PCA fragments (GLuc) [27].

    Polymerase Chain Reaction:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. PREP mutants (D149A-PREP, T202C-PREP, T204A-PREP, T590C-PREP, H680APREP and LoopA-PREP), PP2Ac (HG10420-CF, Sino-Biological), PME1 (SinoBiological) and PTPA (HG12287-UT, Sino-Biological) cDNA was PCR amplified to create insert oligonucleotides with 15 bp overhangs. .. Inserts were gel purified and recombined with EcoRI-HF (NEB) and KpnI-HF (NEB) digested and gel purified GLuc1N, Gluc-2N, GLuc-1C, and GLuc-2C backbone using In-Fusion cloning kit (Clontech).

    Sequencing:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. Similarly, pAAV-EF1a control vector with 50 bp insert was created by annealing complementary oligonucleotides with 15 bp overhangs (10x annealing buffer: 100 nM Tris HCl, 500 mM NaCl, 10 mM EDTA) and recombined with In-Fusion HD cloning kit into the pAAV-EF1a-PREP backbone. pCMV3-PPP2AC-C-FLAG (HG10420-CF), pCMV3-N-FLAG-PPP2AC (HG10420NF), pCMV3-PPP2R4A (HG12287-UT), and pCMV3-N-FLAG-PPP2R4A (HG12287NF) were purchased from Sino-Biological. pAAV-EF1a-FLAG(C)-PME1 and pAAVEF1a-PME1-FLAG(N) inserts were amplified from pUC19-PPME1 (HG29687-U, SinoJo ur na l re -p ro of Biological) with overhangs containing either N- or C-terminal FLAG sequence. pAAV1EF1α-PREP backbone was digested with KpnI-HF (NEB) and EcoRV-HF (NEB), gel purified and recombined with all of the aforementioned inserts using an In-Fusion HD cloning kit (Clontech). .. For protein-fragment complementation assay (PCA), cDNA of PREP, all of the PREP mutants, PP2Ac, PME1 and PTPA were cloned in a pcDNA3.1/zeo backbone containing humanized G. princeps PCA fragments (GLuc) [27].

    Plasmid Preparation:

    Article Title: Prolyl oligopeptidase inhibition activates autophagy via protein phosphatase 2A.
    Article Snippet: .. Similarly, pAAV-EF1a control vector with 50 bp insert was created by annealing complementary oligonucleotides with 15 bp overhangs (10x annealing buffer: 100 nM Tris HCl, 500 mM NaCl, 10 mM EDTA) and recombined with In-Fusion HD cloning kit into the pAAV-EF1a-PREP backbone. pCMV3-PPP2AC-C-FLAG (HG10420-CF), pCMV3-N-FLAG-PPP2AC (HG10420NF), pCMV3-PPP2R4A (HG12287-UT), and pCMV3-N-FLAG-PPP2R4A (HG12287NF) were purchased from Sino-Biological. pAAV-EF1a-FLAG(C)-PME1 and pAAVEF1a-PME1-FLAG(N) inserts were amplified from pUC19-PPME1 (HG29687-U, SinoJo ur na l re -p ro of Biological) with overhangs containing either N- or C-terminal FLAG sequence. pAAV1EF1α-PREP backbone was digested with KpnI-HF (NEB) and EcoRV-HF (NEB), gel purified and recombined with all of the aforementioned inserts using an In-Fusion HD cloning kit (Clontech). .. For protein-fragment complementation assay (PCA), cDNA of PREP, all of the PREP mutants, PP2Ac, PME1 and PTPA were cloned in a pcDNA3.1/zeo backbone containing humanized G. princeps PCA fragments (GLuc) [27].

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