cytomegalovirus pcmv mtor his  (Sino Biological)


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    Name:
    Human cytomegalovirus Glycoprotein B gB HEK293 Cell Lysate
    Description:
    Human Cell lysate that CMV Glycoprotein B transfected overexpressed for Western blot WB positive control The whole cell lysate is provided in 1X Sample Buffer 1X modified RIPA buffer 1X SDS loading buffer
    Catalog Number:
    10202-v08h1l
    Price:
    195
    Applications:
    WB
    Size:
    300µg
    Category:
    Lysate
    Cell Type:
    HEK293 Cells
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    Structured Review

    Sino Biological cytomegalovirus pcmv mtor his
    Human Cell lysate that CMV Glycoprotein B transfected overexpressed for Western blot WB positive control The whole cell lysate is provided in 1X Sample Buffer 1X modified RIPA buffer 1X SDS loading buffer
    https://www.bioz.com/result/cytomegalovirus pcmv mtor his/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cytomegalovirus pcmv mtor his - by Bioz Stars, 2021-02
    94/100 stars

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    Related Articles

    Cell Culture:

    Article Title: An OB-fold complex controls the repair pathways for DNA double-strand breaks
    Article Snippet: .. HEK293 suspension cells were cultured in SMM 293-TI medium (Sino Biological Inc.) supplemented with 1% Gibco FBS and 1% glutamine in an incubator with shaking at 140 r.p.m. ..

    Purification:

    Article Title: Human cytomegalovirus induces a distinct innate immune response in the maternal-fetal interface.
    Article Snippet: .. Specific treatments HCMV glycoprotein B (gB): decidual organ cultures were incubated with 2 mg/ml soluble recombinant HCMV gB (Sino biological Inc. and an additional unrelated baculovirus-insect cell expressed and purified soluble gB, kindly provided by Yarden Opatowsky, Bar-Ilan University), or control glycosylated bovine serum albumin (BSA; Promega). .. HCMV hyperimmune globulins (HIG) and heparin: HCMV (5 105 PFU/ml of purified virus) was pre-incubated with 10 mg/ ml HCMV HIG (Megalotect; Biotest) or 500 IU/ml heparin (SigmaAldrich) for 1 h, prior to inoculation.

    Article Title: Cross-reactivity between avian influenza A (H7N9) virus and divergent H7 subtypic- and heterosubtypic influenza A viruses
    Article Snippet: .. The HA proteins of 13 subtypes of influenza virus, including H1, H2, H3, H4, H5, H6, H8, H9, H10, H11, H12, H13, and H16 , were expressed in HEK-293 cells or in a baculovirus-insect cell system and purified using a 6× His-tag (Sino Biological, Beijing, China). .. The hemagglutinin activities of the recombinant HA proteins were confirmed using the Octet system (ForteBio, CA) and the hemagglutination assay (data not shown).

    Incubation:

    Article Title: Human cytomegalovirus induces a distinct innate immune response in the maternal-fetal interface.
    Article Snippet: .. Specific treatments HCMV glycoprotein B (gB): decidual organ cultures were incubated with 2 mg/ml soluble recombinant HCMV gB (Sino biological Inc. and an additional unrelated baculovirus-insect cell expressed and purified soluble gB, kindly provided by Yarden Opatowsky, Bar-Ilan University), or control glycosylated bovine serum albumin (BSA; Promega). .. HCMV hyperimmune globulins (HIG) and heparin: HCMV (5 105 PFU/ml of purified virus) was pre-incubated with 10 mg/ ml HCMV HIG (Megalotect; Biotest) or 500 IU/ml heparin (SigmaAldrich) for 1 h, prior to inoculation.

    Expressing:

    Article Title: Infection-derived lipids elicit an immune deficiency circuit in arthropods
    Article Snippet: .. Co-immunoprecipitation assayThe expression of both XIAP-HA and Bendless-FLAG in HEK293 T cells was validated with α-HA (Sino Biological, 100028-MM10, 1: 1,000) and α-FLAG (Sigma, F3165, 1: 1,000). .. 2 mg of cell lysates were incubated with 80 μL of either cross-linked α-FLAG M2 agarose beads (Sigma, A2220) or α-HA agarose beads (Pierce, 26181) at 4 °C overnight.

    Article Title: Superior immune responses induced by intranasal immunization with recombinant adenovirus-based vaccine expressing full-length Spike protein of Middle East respiratory syndrome coronavirus
    Article Snippet: .. The expression and secretion of Spike and RBD proteins by rAd-infected HEK293 cells were verified via Western blot analysis by employing anti-MERS-CoV S1 protein rabbit polyclonal antibody [amino acids (aa) 1–725; 40069-T48; Sino Biological Inc., Beijing, China] and HRP-conjugated goat anti-rabbit IgG antibody (Abcam, Cambridge, UK). .. The expression and secretion of NTD protein were validated by infecting HEK293 cells and by conducting Western blot analysis using rAd/Spike-immunized mouse serum (1:1,000 dilution) and HRP-conjugated rabbit anti-mouse IgG antibody (Abcam) as a secondary antibody.

    Western Blot:

    Article Title: Superior immune responses induced by intranasal immunization with recombinant adenovirus-based vaccine expressing full-length Spike protein of Middle East respiratory syndrome coronavirus
    Article Snippet: .. The expression and secretion of Spike and RBD proteins by rAd-infected HEK293 cells were verified via Western blot analysis by employing anti-MERS-CoV S1 protein rabbit polyclonal antibody [amino acids (aa) 1–725; 40069-T48; Sino Biological Inc., Beijing, China] and HRP-conjugated goat anti-rabbit IgG antibody (Abcam, Cambridge, UK). .. The expression and secretion of NTD protein were validated by infecting HEK293 cells and by conducting Western blot analysis using rAd/Spike-immunized mouse serum (1:1,000 dilution) and HRP-conjugated rabbit anti-mouse IgG antibody (Abcam) as a secondary antibody.

    Recombinant:

    Article Title: Human cytomegalovirus induces a distinct innate immune response in the maternal-fetal interface.
    Article Snippet: .. Specific treatments HCMV glycoprotein B (gB): decidual organ cultures were incubated with 2 mg/ml soluble recombinant HCMV gB (Sino biological Inc. and an additional unrelated baculovirus-insect cell expressed and purified soluble gB, kindly provided by Yarden Opatowsky, Bar-Ilan University), or control glycosylated bovine serum albumin (BSA; Promega). .. HCMV hyperimmune globulins (HIG) and heparin: HCMV (5 105 PFU/ml of purified virus) was pre-incubated with 10 mg/ ml HCMV HIG (Megalotect; Biotest) or 500 IU/ml heparin (SigmaAldrich) for 1 h, prior to inoculation.

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  • 93
    Sino Biological human jak2
    Knockout of Shp2 decreases <t>JAK2/STAT3</t> signaling. a Knockout of Shp2 in fibroblasts ( Shp2 fl/fl x Col1a2 ; Cre ER) decreases the levels of pJAK2 and pSTAT3 and STAT3 reporter activity in cultured fibroblasts ( n = 3 different lines). Cells were stimulated with TGFβ (10 ng/ml for 6 h). b – d Conditional knockout of Shp2 reduces the levels of pJAK2 and pSTAT3 in TBRI CA (6.67 × 10 7 IFUs every 2 weeks) ( b ) and bleomycin-induced fibrosis (50 µg every other day) ( c ) and in TSK1 mice (2 mg tamoxifen over 5 days) ( d ) ( n ≥ 3). Results shown are representative of three independent experiments. All data are presented as median ± s.e.m. The p values are expressed as follows: 0.05 > p > 0.01* or # ; 0.01 > p > 0.001** or ## ; p
    Human Jak2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human jak2/product/Sino Biological
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    human jak2 - by Bioz Stars, 2021-02
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    93
    Sino Biological ha suv39h1 plasmids
    Desumoylation of p53 is needed for heterochromatin-mediated p53 target gene repression. ( A ) <t>FLAG-SUV39H1</t> was transfected into hRPE cells. The endogenous p53 protein was precipitated by p53 antibody DO-1, and a 67-kDa form of p53 was detected (labeled with *). The co-IP experiment was conducted in the presence of a desumoylation inhibitor, 20 mM NEM. ( B ) The 67-kDa p53 decreases on OS exposure. WB analysis showing the conventional p53 and a 67-kDa species. Quantification of the percentage of the 67-kDa p53 species (*p53) relative to the total p53. n = 3. ** P
    Ha Suv39h1 Plasmids, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ha suv39h1 plasmids/product/Sino Biological
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ha suv39h1 plasmids - by Bioz Stars, 2021-02
    93/100 stars
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    90
    Sino Biological ace cdna orf clone human untagged
    Desumoylation of p53 is needed for heterochromatin-mediated p53 target gene repression. ( A ) <t>FLAG-SUV39H1</t> was transfected into hRPE cells. The endogenous p53 protein was precipitated by p53 antibody DO-1, and a 67-kDa form of p53 was detected (labeled with *). The co-IP experiment was conducted in the presence of a desumoylation inhibitor, 20 mM NEM. ( B ) The 67-kDa p53 decreases on OS exposure. WB analysis showing the conventional p53 and a 67-kDa species. Quantification of the percentage of the 67-kDa p53 species (*p53) relative to the total p53. n = 3. ** P
    Ace Cdna Orf Clone Human Untagged, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ace cdna orf clone human untagged/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ace cdna orf clone human untagged - by Bioz Stars, 2021-02
    90/100 stars
      Buy from Supplier

    Image Search Results


    Knockout of Shp2 decreases JAK2/STAT3 signaling. a Knockout of Shp2 in fibroblasts ( Shp2 fl/fl x Col1a2 ; Cre ER) decreases the levels of pJAK2 and pSTAT3 and STAT3 reporter activity in cultured fibroblasts ( n = 3 different lines). Cells were stimulated with TGFβ (10 ng/ml for 6 h). b – d Conditional knockout of Shp2 reduces the levels of pJAK2 and pSTAT3 in TBRI CA (6.67 × 10 7 IFUs every 2 weeks) ( b ) and bleomycin-induced fibrosis (50 µg every other day) ( c ) and in TSK1 mice (2 mg tamoxifen over 5 days) ( d ) ( n ≥ 3). Results shown are representative of three independent experiments. All data are presented as median ± s.e.m. The p values are expressed as follows: 0.05 > p > 0.01* or # ; 0.01 > p > 0.001** or ## ; p

    Journal: Nature Communications

    Article Title: The tyrosine phosphatase SHP2 controls TGFβ-induced STAT3 signaling to regulate fibroblast activation and fibrosis

    doi: 10.1038/s41467-018-05768-3

    Figure Lengend Snippet: Knockout of Shp2 decreases JAK2/STAT3 signaling. a Knockout of Shp2 in fibroblasts ( Shp2 fl/fl x Col1a2 ; Cre ER) decreases the levels of pJAK2 and pSTAT3 and STAT3 reporter activity in cultured fibroblasts ( n = 3 different lines). Cells were stimulated with TGFβ (10 ng/ml for 6 h). b – d Conditional knockout of Shp2 reduces the levels of pJAK2 and pSTAT3 in TBRI CA (6.67 × 10 7 IFUs every 2 weeks) ( b ) and bleomycin-induced fibrosis (50 µg every other day) ( c ) and in TSK1 mice (2 mg tamoxifen over 5 days) ( d ) ( n ≥ 3). Results shown are representative of three independent experiments. All data are presented as median ± s.e.m. The p values are expressed as follows: 0.05 > p > 0.01* or # ; 0.01 > p > 0.001** or ## ; p

    Article Snippet: The plasmid pCMV3-Flag-JAK2 encoding the human JAK2 was purchased from Sino Biological (Beijing, China).

    Techniques: Knock-Out, Activity Assay, Cell Culture, Mouse Assay

    Inhibition of SHP2 limits JAK2/STAT3 signaling and fibroblast activation. a Changes in the mRNA levels of COL1A1 and of collagen protein in human fibroblasts incubated with increasing doses of NSC-87877 (10 µM, 30 µM and 100 µM). Fibroblasts were treated with TGFβ (10 ng/ml) for 24 h. b ACTA2 mRNA. ( n ≥ 4) c Representative images of immunofluorescence stainings for α-SMA and stress fiber staining are shown at 400-fold magnification and quantification of α-SMA staining intensity as well as stress fiber staining intensity ( n ≥ 15) (TGFβ 10 ng/ml for 24 h). Horizontal scale bar, 500 μm. d Representative western blots for pJAK2 Y1007/Y1008 , pJAK2 Y570 , total JAK2, pSTAT3 Y705 and total STAT3 with β-actin as loading control and quantification of the results (TGFβ 10 ng/ml for 6 h) ( n ≥ 2). e Changes in STAT3 reporter activity ( n ≥ 6) (TGFβ 10 ng/ml for 6 h). Results shown are representative of three independent experiments All data are presented as median ± s.e.m. The p values are expressed as follows: 0.05 > p > 0.01*; 0.01 > p > 0.001**; p

    Journal: Nature Communications

    Article Title: The tyrosine phosphatase SHP2 controls TGFβ-induced STAT3 signaling to regulate fibroblast activation and fibrosis

    doi: 10.1038/s41467-018-05768-3

    Figure Lengend Snippet: Inhibition of SHP2 limits JAK2/STAT3 signaling and fibroblast activation. a Changes in the mRNA levels of COL1A1 and of collagen protein in human fibroblasts incubated with increasing doses of NSC-87877 (10 µM, 30 µM and 100 µM). Fibroblasts were treated with TGFβ (10 ng/ml) for 24 h. b ACTA2 mRNA. ( n ≥ 4) c Representative images of immunofluorescence stainings for α-SMA and stress fiber staining are shown at 400-fold magnification and quantification of α-SMA staining intensity as well as stress fiber staining intensity ( n ≥ 15) (TGFβ 10 ng/ml for 24 h). Horizontal scale bar, 500 μm. d Representative western blots for pJAK2 Y1007/Y1008 , pJAK2 Y570 , total JAK2, pSTAT3 Y705 and total STAT3 with β-actin as loading control and quantification of the results (TGFβ 10 ng/ml for 6 h) ( n ≥ 2). e Changes in STAT3 reporter activity ( n ≥ 6) (TGFβ 10 ng/ml for 6 h). Results shown are representative of three independent experiments All data are presented as median ± s.e.m. The p values are expressed as follows: 0.05 > p > 0.01*; 0.01 > p > 0.001**; p

    Article Snippet: The plasmid pCMV3-Flag-JAK2 encoding the human JAK2 was purchased from Sino Biological (Beijing, China).

    Techniques: Inhibition, Activation Assay, Incubation, Immunofluorescence, Staining, Western Blot, Activity Assay

    SHP2 enhances TGFβ-induced fibroblast activation via JAK2/STAT3. a mRNA levels of SHP2 after overexpression in human dermal fibroblasts. mRNA levels of COL1A1 in human fibroblasts transfected with empty vector, SHP2 WT - and SHP2 C459S -expression vectors, with or without TGFβ1 treatment (10 ng/ml for 24 h) ( n ≥ 4). b Western blot analysis and respective quantifications for type I collagen and SHP2 in human fibroblasts transfected with empty vector, SHP2 WT - and SHP2 C459S -expression vectors, with or without TGFβ1 treatment (10 ng/ml for 24 h). Western blot for pJAK2 Y1007/Y1008 , pJAK2 Y570 , total JAK2, pSTAT3 Y705 and total STAT3 with β-actin as loading control (TGFβ 10 ng/ml for 6 h) ( n = 3). Results shown are representative of three independent experiments. c , d Representative images of immunofluorescence stainings for α-SMA and stress fiber staining are shown at 400-fold magnification ( c ) and quantification of α-SMA staining intensity as well as stress fiber staining intensity ( d ) ( n ≥ 4). Horizontal scale bar, 500 μm. All data are presented as median ± s.e.m. The p values are expressed as follows: 0.05 > p > 0.01*; 0.01 > p > 0.001**; p

    Journal: Nature Communications

    Article Title: The tyrosine phosphatase SHP2 controls TGFβ-induced STAT3 signaling to regulate fibroblast activation and fibrosis

    doi: 10.1038/s41467-018-05768-3

    Figure Lengend Snippet: SHP2 enhances TGFβ-induced fibroblast activation via JAK2/STAT3. a mRNA levels of SHP2 after overexpression in human dermal fibroblasts. mRNA levels of COL1A1 in human fibroblasts transfected with empty vector, SHP2 WT - and SHP2 C459S -expression vectors, with or without TGFβ1 treatment (10 ng/ml for 24 h) ( n ≥ 4). b Western blot analysis and respective quantifications for type I collagen and SHP2 in human fibroblasts transfected with empty vector, SHP2 WT - and SHP2 C459S -expression vectors, with or without TGFβ1 treatment (10 ng/ml for 24 h). Western blot for pJAK2 Y1007/Y1008 , pJAK2 Y570 , total JAK2, pSTAT3 Y705 and total STAT3 with β-actin as loading control (TGFβ 10 ng/ml for 6 h) ( n = 3). Results shown are representative of three independent experiments. c , d Representative images of immunofluorescence stainings for α-SMA and stress fiber staining are shown at 400-fold magnification ( c ) and quantification of α-SMA staining intensity as well as stress fiber staining intensity ( d ) ( n ≥ 4). Horizontal scale bar, 500 μm. All data are presented as median ± s.e.m. The p values are expressed as follows: 0.05 > p > 0.01*; 0.01 > p > 0.001**; p

    Article Snippet: The plasmid pCMV3-Flag-JAK2 encoding the human JAK2 was purchased from Sino Biological (Beijing, China).

    Techniques: Activation Assay, Over Expression, Transfection, Plasmid Preparation, Expressing, Western Blot, Immunofluorescence, Staining

    Overexpression of JAK2 ∆Y570F prevents the inhibitory effects of SHP2 inhibitors on TGFβ-induced fibroblast activation. a mRNA levels of COL1A1 and COL1A2 (TGFβ 10 ng/ml for 24 h) ( n ≥ 5). b Release of collagen protein (TGFβ 10 ng/ml for 24 h) ( n ≥ 6). c Representative images of immunofluorescence stainings for α-SMA and stress fiber at 400-fold magnification and respective quantifications (TGFβ 10 ng/ml for 24 h) ( n ≥ 6). Horizontal scale bar, 500 μm. d STAT3 reporter Assay upon JAK2 WT and Y570F mutant overexpression. Cells were treated with TGFβ (10 ng/ml for 6 h) and NSC-87877 (100 µM) ( n ≥ 4). e Co-immunoprecipitation and respective quantifications of endogenous JAK2 with endogenous SHP2 in human fibroblasts stimulated with TGFβ (10 ng/ml for 30′) ( n = 3). Results shown are representative of ≥ 3 independent experiments. All data are presented as median ± s.e.m. The p values are expressed as follows: 0.05 > p > 0.01*; 0.01 > p > 0.001**; p

    Journal: Nature Communications

    Article Title: The tyrosine phosphatase SHP2 controls TGFβ-induced STAT3 signaling to regulate fibroblast activation and fibrosis

    doi: 10.1038/s41467-018-05768-3

    Figure Lengend Snippet: Overexpression of JAK2 ∆Y570F prevents the inhibitory effects of SHP2 inhibitors on TGFβ-induced fibroblast activation. a mRNA levels of COL1A1 and COL1A2 (TGFβ 10 ng/ml for 24 h) ( n ≥ 5). b Release of collagen protein (TGFβ 10 ng/ml for 24 h) ( n ≥ 6). c Representative images of immunofluorescence stainings for α-SMA and stress fiber at 400-fold magnification and respective quantifications (TGFβ 10 ng/ml for 24 h) ( n ≥ 6). Horizontal scale bar, 500 μm. d STAT3 reporter Assay upon JAK2 WT and Y570F mutant overexpression. Cells were treated with TGFβ (10 ng/ml for 6 h) and NSC-87877 (100 µM) ( n ≥ 4). e Co-immunoprecipitation and respective quantifications of endogenous JAK2 with endogenous SHP2 in human fibroblasts stimulated with TGFβ (10 ng/ml for 30′) ( n = 3). Results shown are representative of ≥ 3 independent experiments. All data are presented as median ± s.e.m. The p values are expressed as follows: 0.05 > p > 0.01*; 0.01 > p > 0.001**; p

    Article Snippet: The plasmid pCMV3-Flag-JAK2 encoding the human JAK2 was purchased from Sino Biological (Beijing, China).

    Techniques: Over Expression, Activation Assay, Immunofluorescence, Reporter Assay, Mutagenesis, Immunoprecipitation

    Desumoylation of p53 is needed for heterochromatin-mediated p53 target gene repression. ( A ) FLAG-SUV39H1 was transfected into hRPE cells. The endogenous p53 protein was precipitated by p53 antibody DO-1, and a 67-kDa form of p53 was detected (labeled with *). The co-IP experiment was conducted in the presence of a desumoylation inhibitor, 20 mM NEM. ( B ) The 67-kDa p53 decreases on OS exposure. WB analysis showing the conventional p53 and a 67-kDa species. Quantification of the percentage of the 67-kDa p53 species (*p53) relative to the total p53. n = 3. ** P

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Heterochromatin protects retinal pigment epithelium cells from oxidative damage by silencing p53 target genes

    doi: 10.1073/pnas.1715237115

    Figure Lengend Snippet: Desumoylation of p53 is needed for heterochromatin-mediated p53 target gene repression. ( A ) FLAG-SUV39H1 was transfected into hRPE cells. The endogenous p53 protein was precipitated by p53 antibody DO-1, and a 67-kDa form of p53 was detected (labeled with *). The co-IP experiment was conducted in the presence of a desumoylation inhibitor, 20 mM NEM. ( B ) The 67-kDa p53 decreases on OS exposure. WB analysis showing the conventional p53 and a 67-kDa species. Quantification of the percentage of the 67-kDa p53 species (*p53) relative to the total p53. n = 3. ** P

    Article Snippet: FLAG-SUV39H1 and HA-SUV39H1 plasmids were purchased from Sino Biological and verified by sequencing.

    Techniques: Transfection, Labeling, Co-Immunoprecipitation Assay, Western Blot