negr1  (Sino Biological)


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    Name:
    NEGR1 cDNA ORF Clone in Cloning Vector Human
    Description:
    Full length Clone DNA of Human neuronal growth regulator 1
    Catalog Number:
    hg11181-m
    Product Aliases:
    DMML2433 cDNA ORF Clone Human, IGLON4 cDNA ORF Clone Human, KILON cDNA ORF Clone Human, Ntra cDNA ORF Clone Human
    Price:
    75.0
    Size:
    1Unit
    Category:
    cDNA Clone
    Molecule Name:
    NEGR1,Ntra,
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    Structured Review

    Sino Biological negr1
    Full length Clone DNA of Human neuronal growth regulator 1
    https://www.bioz.com/result/negr1/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    negr1 - by Bioz Stars, 2021-02
    86/100 stars

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    Related Articles

    Transfection:

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: .. The constructs, corresponding to the sequence encoding the ectodomain of NTM, OBCAM, LSAMP, and NEGR1 subcloned into the PFUSE vector, were transiently transfected into HEK293T cells with calcium phosphate ( ). .. Transfected cells were incubated in the serum-free medium, OptiMEM (Invitrogen), and the media were collected 4 days post-transfection.

    In Vivo:

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: .. To validate the processing of NTM and NEGR1 by metalloproteinases in vivo , we collected the conditioned media from brain extracts. .. All IgLON family members were detected in the conditioned media, and similar to the culture experiment, NTM and NEGR1 were most robustly shed ( F ).

    Cell Culture:

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: .. Overexpression of individual IgLON family members differentially affects the number of dendritic synapses that form in cultured hippocampal neurons, whereas NEGR1 and OBCAM loss of function results in a reduction in synapse formation on dendrites ( , , ). ..

    other:

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: Our data also support the idea that shedding of NTM and NEGR1 acts to relieve an outgrowth inhibitory signal transduced from the cell surface.

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: All IgLON family members were detected in the conditioned media, and similar to the culture experiment, NTM and NEGR1 were most robustly shed ( F ).

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: Introduction of NTM and NEGR1 into 2-DIV cortical neurons, which do not express endogenous IgLON at the cell surface, sensitized neurons to the outgrowth inhibitory effect of BB-94 but did not affect outgrowth in the absence of BB-94 ( ).

    Activity Assay:

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: .. Introduction of NTM or NEGR1, but not OBCAM or LSAMP, was sufficient to sensitize these neurons to the outgrowth inhibitory activity of the metalloproteinase inhibitor BB-94 implicating shedding of these IgLON family members in regulating the intrinsic growth capacity of neurons ( B ). .. Introduction of the IgLONs in the absence of BB-94 did not impact outgrowth suggesting that BB-94 leads to an accumulation of NTM and NEGR1 on the cell surface that impedes neurite extension.

    Construct:

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: .. The constructs, corresponding to the sequence encoding the ectodomain of NTM, OBCAM, LSAMP, and NEGR1 subcloned into the PFUSE vector, were transiently transfected into HEK293T cells with calcium phosphate ( ). .. Transfected cells were incubated in the serum-free medium, OptiMEM (Invitrogen), and the media were collected 4 days post-transfection.

    Sequencing:

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: .. The constructs, corresponding to the sequence encoding the ectodomain of NTM, OBCAM, LSAMP, and NEGR1 subcloned into the PFUSE vector, were transiently transfected into HEK293T cells with calcium phosphate ( ). .. Transfected cells were incubated in the serum-free medium, OptiMEM (Invitrogen), and the media were collected 4 days post-transfection.

    Western Blot:

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: .. In contrast, only NTM and NEGR1 could be detected in conditioned media from cortical neurons by Western blotting. ..

    Over Expression:

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: .. Overexpression of individual IgLON family members differentially affects the number of dendritic synapses that form in cultured hippocampal neurons, whereas NEGR1 and OBCAM loss of function results in a reduction in synapse formation on dendrites ( , , ). ..

    Plasmid Preparation:

    Article Title: IgLON Cell Adhesion Molecules Are Shed from the Cell Surface of Cortical Neurons to Promote Neuronal Growth *
    Article Snippet: .. The constructs, corresponding to the sequence encoding the ectodomain of NTM, OBCAM, LSAMP, and NEGR1 subcloned into the PFUSE vector, were transiently transfected into HEK293T cells with calcium phosphate ( ). .. Transfected cells were incubated in the serum-free medium, OptiMEM (Invitrogen), and the media were collected 4 days post-transfection.

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  • 86
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