hcrtr2 transcription (Sino Biological)


Name:
HCRTR2 OX2R cDNA ORF Clone Human untagged
Description:
Full length Clone DNA of Human hypocretin orexin receptor 2
Catalog Number:
hg10844-ut
Product Aliases:
OX2R cDNA ORF Clone Human
Price:
195.0
Applications:
Stable or Transient mammalian expression
Size:
1Unit
Category:
cDNA Clone
Molecule Name:
HCRTR2,OX2R,HCRTR2
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Full length Clone DNA of Human hypocretin orexin receptor 2
https://www.bioz.com/result/hcrtr2 transcription/product/Sino Biological
Average 90 stars, based on 3 article reviews
Price from $9.99 to $1999.99
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Images
1) Product Images from "Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases"
Article Title: Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases
Journal: PLoS ONE
doi: 10.1371/journal.pone.0187305
![Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of ... Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.](https://storage.googleapis.com/bioz_article_images/PMC5722318/pone.0187305.g004.jpg)
Figure Legend Snippet: Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.
Techniques Used: Flow Cytometry, Cytometry, Binding Assay, In-Cell ELISA

Figure Legend Snippet: Anti-HCRTR2 autoantibody detection in human sera using flow cytometry. HEK293T cells with transient expression of HCRTR2-GFP were stained with positive anti-HCRTR2 antibodies (Ab) at different dilution ratios (A-D) or human sera (1:20) (E-L), followed by staining with Alexa Fluor ® 555 (AF555)-conjugated anti-mouse IgG or anti-human IgG (1:100), respectively. 50,000 events were recorded and dot plots of live single cells were shown with GFP channel (X axis) and AF555 channel (Y axis) for each sample. ΔMFI AF555 (mean fluorescence intensity (MFI) of AF555 channel) was determined by subtracting MFI AF555 of HEK293T GFP- from MFI AF555 of HEK293T GFP+ . Database identity (DbID) of each subject was also shown. P, patient; C, control.
Techniques Used: Flow Cytometry, Cytometry, Expressing, Staining, Fluorescence
2) Product Images from "Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases"
Article Title: Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases
Journal: PLoS ONE
doi: 10.1371/journal.pone.0187305
![Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of ... Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.](https://storage.googleapis.com/bioz_article_images/PMC5722318/pone.0187305.g004.jpg)
Figure Legend Snippet: Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.
Techniques Used: Flow Cytometry, Cytometry, Binding Assay, In-Cell ELISA

Figure Legend Snippet: Anti-HCRTR2 autoantibody detection in human sera using flow cytometry. HEK293T cells with transient expression of HCRTR2-GFP were stained with positive anti-HCRTR2 antibodies (Ab) at different dilution ratios (A-D) or human sera (1:20) (E-L), followed by staining with Alexa Fluor ® 555 (AF555)-conjugated anti-mouse IgG or anti-human IgG (1:100), respectively. 50,000 events were recorded and dot plots of live single cells were shown with GFP channel (X axis) and AF555 channel (Y axis) for each sample. ΔMFI AF555 (mean fluorescence intensity (MFI) of AF555 channel) was determined by subtracting MFI AF555 of HEK293T GFP- from MFI AF555 of HEK293T GFP+ . Database identity (DbID) of each subject was also shown. P, patient; C, control.
Techniques Used: Flow Cytometry, Cytometry, Expressing, Staining, Fluorescence