hcrtr2 transcription  (Sino Biological)


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    Name:
    HCRTR2 OX2R cDNA ORF Clone Human untagged
    Description:
    Full length Clone DNA of Human hypocretin orexin receptor 2
    Catalog Number:
    hg10844-ut
    Product Aliases:
    OX2R cDNA ORF Clone Human
    Price:
    195.0
    Applications:
    Stable or Transient mammalian expression
    Size:
    1Unit
    Category:
    cDNA Clone
    Molecule Name:
    HCRTR2,OX2R,HCRTR2
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    Structured Review

    Sino Biological hcrtr2 transcription
    HCRTR2 OX2R cDNA ORF Clone Human untagged
    Full length Clone DNA of Human hypocretin orexin receptor 2
    https://www.bioz.com/result/hcrtr2 transcription/product/Sino Biological
    Average 90 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    hcrtr2 transcription - by Bioz Stars, 2021-02
    90/100 stars

    Images

    1) Product Images from "Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases"

    Article Title: Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0187305

    Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.
    Figure Legend Snippet: Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.

    Techniques Used: Flow Cytometry, Cytometry, Binding Assay, In-Cell ELISA

    Anti-HCRTR2 autoantibody detection in human sera using flow cytometry. HEK293T cells with transient expression of HCRTR2-GFP were stained with positive anti-HCRTR2 antibodies (Ab) at different dilution ratios (A-D) or human sera (1:20) (E-L), followed by staining with Alexa Fluor ® 555 (AF555)-conjugated anti-mouse IgG or anti-human IgG (1:100), respectively. 50,000 events were recorded and dot plots of live single cells were shown with GFP channel (X axis) and AF555 channel (Y axis) for each sample. ΔMFI AF555 (mean fluorescence intensity (MFI) of AF555 channel) was determined by subtracting MFI AF555 of HEK293T GFP- from MFI AF555 of HEK293T GFP+ . Database identity (DbID) of each subject was also shown. P, patient; C, control.
    Figure Legend Snippet: Anti-HCRTR2 autoantibody detection in human sera using flow cytometry. HEK293T cells with transient expression of HCRTR2-GFP were stained with positive anti-HCRTR2 antibodies (Ab) at different dilution ratios (A-D) or human sera (1:20) (E-L), followed by staining with Alexa Fluor ® 555 (AF555)-conjugated anti-mouse IgG or anti-human IgG (1:100), respectively. 50,000 events were recorded and dot plots of live single cells were shown with GFP channel (X axis) and AF555 channel (Y axis) for each sample. ΔMFI AF555 (mean fluorescence intensity (MFI) of AF555 channel) was determined by subtracting MFI AF555 of HEK293T GFP- from MFI AF555 of HEK293T GFP+ . Database identity (DbID) of each subject was also shown. P, patient; C, control.

    Techniques Used: Flow Cytometry, Cytometry, Expressing, Staining, Fluorescence

    2) Product Images from "Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases"

    Article Title: Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0187305

    Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.
    Figure Legend Snippet: Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.

    Techniques Used: Flow Cytometry, Cytometry, Binding Assay, In-Cell ELISA

    Anti-HCRTR2 autoantibody detection in human sera using flow cytometry. HEK293T cells with transient expression of HCRTR2-GFP were stained with positive anti-HCRTR2 antibodies (Ab) at different dilution ratios (A-D) or human sera (1:20) (E-L), followed by staining with Alexa Fluor ® 555 (AF555)-conjugated anti-mouse IgG or anti-human IgG (1:100), respectively. 50,000 events were recorded and dot plots of live single cells were shown with GFP channel (X axis) and AF555 channel (Y axis) for each sample. ΔMFI AF555 (mean fluorescence intensity (MFI) of AF555 channel) was determined by subtracting MFI AF555 of HEK293T GFP- from MFI AF555 of HEK293T GFP+ . Database identity (DbID) of each subject was also shown. P, patient; C, control.
    Figure Legend Snippet: Anti-HCRTR2 autoantibody detection in human sera using flow cytometry. HEK293T cells with transient expression of HCRTR2-GFP were stained with positive anti-HCRTR2 antibodies (Ab) at different dilution ratios (A-D) or human sera (1:20) (E-L), followed by staining with Alexa Fluor ® 555 (AF555)-conjugated anti-mouse IgG or anti-human IgG (1:100), respectively. 50,000 events were recorded and dot plots of live single cells were shown with GFP channel (X axis) and AF555 channel (Y axis) for each sample. ΔMFI AF555 (mean fluorescence intensity (MFI) of AF555 channel) was determined by subtracting MFI AF555 of HEK293T GFP- from MFI AF555 of HEK293T GFP+ . Database identity (DbID) of each subject was also shown. P, patient; C, control.

    Techniques Used: Flow Cytometry, Cytometry, Expressing, Staining, Fluorescence

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    Sino Biological hcrtr2 transcription
    Differential analysis of <t>anti-HCRTR2</t> autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.
    Hcrtr2 Transcription, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hcrtr2 transcription/product/Sino Biological
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    hcrtr2 transcription - by Bioz Stars, 2021-02
    90/100 stars
      Buy from Supplier

    85
    Sino Biological ceacam6 release
    Differential analysis of <t>anti-HCRTR2</t> autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.
    Ceacam6 Release, supplied by Sino Biological, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ceacam6 release/product/Sino Biological
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ceacam6 release - by Bioz Stars, 2021-02
    85/100 stars
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    90
    Sino Biological plasmid pcmv3 ha hcrtr2
    Differential analysis of <t>anti-HCRTR2</t> autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.
    Plasmid Pcmv3 Ha Hcrtr2, supplied by Sino Biological, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid pcmv3 ha hcrtr2/product/Sino Biological
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    plasmid pcmv3 ha hcrtr2 - by Bioz Stars, 2021-02
    90/100 stars
      Buy from Supplier

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    Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.

    Journal: PLoS ONE

    Article Title: Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases

    doi: 10.1371/journal.pone.0187305

    Figure Lengend Snippet: Differential analysis of anti-HCRTR2 autoantibodies using three distinct strategies. Two groups of narcolepsy patients and control subjects were tested using flow cytometry (A), [ 35 S]-radiolabelled HCRTR2 binding assay (B), and in-cell ELISA using CHO-HCRTR2 (C). Each dot corresponded to one patient or control subject. The dotted line denoted cut-off value, the mean ± 3× SD of all healthy control subjects for each method. Values above this threshold were considered positive for anti-HCRTR2 autoantibody reaction. PP-N, post Pandemrix ® narcolepsy patients; EO-N, early onset narcolepsy patients; PP-C, post Pandemrix ® control subjects; O-C, other controls, matched to early onset subjects. The numbers of each group was given. P-values were shown between PP-N and PP-C, and between EO-N and O-C.

    Article Snippet: HCRTR2 constructs For HCRTR2 transcription and translation in vitro , plasmid pCMV3-HA-HCRTR2 was purchased from Sino Biological Inc. (Cat# HG10844-NY-HCRTR2).

    Techniques: Flow Cytometry, Cytometry, Binding Assay, In-Cell ELISA

    Anti-HCRTR2 autoantibody detection in human sera using flow cytometry. HEK293T cells with transient expression of HCRTR2-GFP were stained with positive anti-HCRTR2 antibodies (Ab) at different dilution ratios (A-D) or human sera (1:20) (E-L), followed by staining with Alexa Fluor ® 555 (AF555)-conjugated anti-mouse IgG or anti-human IgG (1:100), respectively. 50,000 events were recorded and dot plots of live single cells were shown with GFP channel (X axis) and AF555 channel (Y axis) for each sample. ΔMFI AF555 (mean fluorescence intensity (MFI) of AF555 channel) was determined by subtracting MFI AF555 of HEK293T GFP- from MFI AF555 of HEK293T GFP+ . Database identity (DbID) of each subject was also shown. P, patient; C, control.

    Journal: PLoS ONE

    Article Title: Absence of anti-hypocretin receptor 2 autoantibodies in post pandemrix narcolepsy cases

    doi: 10.1371/journal.pone.0187305

    Figure Lengend Snippet: Anti-HCRTR2 autoantibody detection in human sera using flow cytometry. HEK293T cells with transient expression of HCRTR2-GFP were stained with positive anti-HCRTR2 antibodies (Ab) at different dilution ratios (A-D) or human sera (1:20) (E-L), followed by staining with Alexa Fluor ® 555 (AF555)-conjugated anti-mouse IgG or anti-human IgG (1:100), respectively. 50,000 events were recorded and dot plots of live single cells were shown with GFP channel (X axis) and AF555 channel (Y axis) for each sample. ΔMFI AF555 (mean fluorescence intensity (MFI) of AF555 channel) was determined by subtracting MFI AF555 of HEK293T GFP- from MFI AF555 of HEK293T GFP+ . Database identity (DbID) of each subject was also shown. P, patient; C, control.

    Article Snippet: HCRTR2 constructs For HCRTR2 transcription and translation in vitro , plasmid pCMV3-HA-HCRTR2 was purchased from Sino Biological Inc. (Cat# HG10844-NY-HCRTR2).

    Techniques: Flow Cytometry, Cytometry, Expressing, Staining, Fluorescence