cd34  (Sino Biological)


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    Name:
    CD34 cDNA ORF Clone in Cloning Vector Human
    Description:
    Full length Clone DNA of Human CD34 molecule transcript variant 2
    Catalog Number:
    hg10097-m
    Price:
    75.0
    Size:
    1Unit
    Category:
    cDNA Clone
    Molecule Name:
    CD34,AU040960,
    Buy from Supplier


    Structured Review

    Sino Biological cd34
    The proportion of <t>CD34</t> + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Full length Clone DNA of Human CD34 molecule transcript variant 2
    https://www.bioz.com/result/cd34/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd34 - by Bioz Stars, 2021-02
    93/100 stars

    Images

    1) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    2) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    3) Product Images from "Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus"

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2020.10922

    TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P
    Figure Legend Snippet: TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P

    Techniques Used: Expressing, Injection, Plasmid Preparation, Isolation, Immunohistochemistry, Staining, Over Expression

    4) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    5) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    6) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    7) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    8) Product Images from "Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus"

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2020.10922

    TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P
    Figure Legend Snippet: TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P

    Techniques Used: Expressing, Injection, Plasmid Preparation, Isolation, Immunohistochemistry, Staining, Over Expression

    9) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    10) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    11) Product Images from "Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus"

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus

    Journal: Molecular Medicine Reports

    doi: 10.3892/mmr.2020.10922

    TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P
    Figure Legend Snippet: TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P

    Techniques Used: Expressing, Injection, Plasmid Preparation, Isolation, Immunohistochemistry, Staining, Over Expression

    12) Product Images from "De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody"

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2016.11.006

    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Figure Legend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Techniques Used: Flow Cytometry, Incubation

    Related Articles

    Immunohistochemistry:

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus
    Article Snippet: .. TIMP3, CD34 and substance P expression levels were determined by immunohistochemical (IHC) staining. .. All staining procedures were performed following standard histochemical protocols.

    Isolation:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: .. 3.6 Effects of various culture conditions on CD34 expression Previous data have shown that freshly isolated HUVECs are 90–95% CD34+ , but CD34 expression is rapidly lost when cells are cultured , . .. In this study, we kept HUVECs in culture (complete ECM) for a period of 7 days without being passaged, the proportion of CD34+ cells strongly increased (39.62%) at passage 8 ( A).

    Cell Culture:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: .. Furthermore, stimulated the 7-day cultured HUVECs with serum starvation in the absence or presence of VEGF165 (25 ng/mL) for 24 h showed a more increased percentage of CD34+ HUVECs, 44.75% ( B) and 60.82% ( C), respectively. .. 3.7 Effects of humanized QBEND/10 on VEGF165 -induced tube formation of endothelial cells The HUVEC tube formation assay is an in vitro angiogenesis assay, which recapitulates some angiogenesis steps and has been used for many years , .

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: .. 3.6 Effects of various culture conditions on CD34 expression Previous data have shown that freshly isolated HUVECs are 90–95% CD34+ , but CD34 expression is rapidly lost when cells are cultured , . .. In this study, we kept HUVECs in culture (complete ECM) for a period of 7 days without being passaged, the proportion of CD34+ cells strongly increased (39.62%) at passage 8 ( A).

    other:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: Therefore, CD34 could be a potential drug target for antiangiogenic therapy.

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: In this study, we kept HUVECs in culture (complete ECM) for a period of 7 days without being passaged, the proportion of CD34+ cells strongly increased (39.62%) at passage 8 ( A).

    Expressing:

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus
    Article Snippet: .. TIMP3, CD34 and substance P expression levels were determined by immunohistochemical (IHC) staining. .. All staining procedures were performed following standard histochemical protocols.

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: .. 3.6 Effects of various culture conditions on CD34 expression Previous data have shown that freshly isolated HUVECs are 90–95% CD34+ , but CD34 expression is rapidly lost when cells are cultured , . .. In this study, we kept HUVECs in culture (complete ECM) for a period of 7 days without being passaged, the proportion of CD34+ cells strongly increased (39.62%) at passage 8 ( A).

    Staining:

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus
    Article Snippet: .. TIMP3, CD34 and substance P expression levels were determined by immunohistochemical (IHC) staining. .. All staining procedures were performed following standard histochemical protocols.

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus
    Article Snippet: .. The puncture group exhibited more positive CD34 and substance P staining, which indicated the neovascularization of IVDs after puncture. .. The positive staining rate of CD34 and substance P was significantly reduced in the TIMP3+puncture group compared with that in the control group ( ).

    Binding Assay:

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: .. The similar KD values of the chimeric and humanized QBEND/10 indicated that the process of humanization did not alter the binding affinity of CD34. .. 3.6 Effects of various culture conditions on CD34 expression Previous data have shown that freshly isolated HUVECs are 90–95% CD34+ , but CD34 expression is rapidly lost when cells are cultured , .

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody
    Article Snippet: .. It was classified as binding to the class II epitope of CD34 . .. The recombinant QBEND/10 IgG has not been reported to date.

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    Sino Biological cd34
    The proportion of <t>CD34</t> + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.
    Cd34, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd34/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cd34 - by Bioz Stars, 2021-02
    93/100 stars
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    The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Journal: Biochemistry and Biophysics Reports

    Article Title: De novo protein sequencing, humanization and in vitro effects of an antihuman CD34 mouse monoclonal antibody

    doi: 10.1016/j.bbrep.2016.11.006

    Figure Lengend Snippet: The proportion of CD34 + HUVECs in different culture conditions. The proportion of CD34 + cells was determined by flow cytometry after 7-day culturing. (A) Cells incubated with complete medium for 24 h. (B) Cells incubated with starvation medium for 24 h. (C) Cells stimulated with VEGF 165 in starvation medium for 24 h.

    Article Snippet: In this study, we kept HUVECs in culture (complete ECM) for a period of 7 days without being passaged, the proportion of CD34+ cells strongly increased (39.62%) at passage 8 ( A).

    Techniques: Flow Cytometry, Incubation

    TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P

    Journal: Molecular Medicine Reports

    Article Title: Overexpression of TIMP3 inhibits discogenic pain by suppressing angiogenesis and the expression of substance P in nucleus pulposus

    doi: 10.3892/mmr.2020.10922

    Figure Lengend Snippet: TIMP3, CD34 and substance P expression in rat NP tissue. Rats were percutaneously punctured with a 21G needle in coccygeal vertebra (puncture and TIMP3+puncture group). For TIMP3+puncture group, rats were injected with adenovirus vector (1×10 9 pfu/level) immediately after puncture. At day 28 after puncture, nucleus pulposus (NP) tissues were isolated for immunohistochemical staining. (A and B) Immunohistochemical staining was performed to measure TIMP3, CD34 and substance P expression in rat NP tissue. Overexpression of TIMP3 could reduce CD34 and substance P expression in NP tissue compared with the puncture group. Black arrow, positive staining. Data are presented as the mean ± SD. *P

    Article Snippet: TIMP3, CD34 and substance P expression levels were determined by immunohistochemical (IHC) staining.

    Techniques: Expressing, Injection, Plasmid Preparation, Isolation, Immunohistochemistry, Staining, Over Expression