max his tagged (Sino Biological)


Name:
UBE2D1 cDNA ORF Clone Human C Myc tag
Description:
Full length Clone DNA of Human ubiquitin conjugating enzyme E2D 1 UBC4 5 homolog yeast with C terminal Myc tag
Catalog Number:
hg11432-cm
Product Aliases:
E2(17)KB1 cDNA ORF Clone Human, SFT cDNA ORF Clone Human, UBC4/5 cDNA ORF Clone Human, UBCH5 cDNA ORF Clone Human, UBCH5A cDNA ORF Clone Human
Price:
195.0
Applications:
Stable or Transient mammalian expression
Size:
1Unit
Category:
cDNA Clone
Molecule Name:
UBE2D1,UBCH5,
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Structured Review

Full length Clone DNA of Human ubiquitin conjugating enzyme E2D 1 UBC4 5 homolog yeast with C terminal Myc tag
https://www.bioz.com/result/max his tagged/product/Sino Biological
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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Images
1) Product Images from "TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation"
Article Title: TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation
Journal: Nature Communications
doi: 10.1038/s41467-020-20107-1

Figure Legend Snippet: TRIB3 interacts with MYC to inhibit the UBE3B:MYC axis. a In vitro ubiquitination assays show that the TRIB3 or TRIB3-KDC protein decreased polyubiquitination of MYC mediated by UBE3B and UBCH3. b The TRIB3 protein or the KDC domain of TRIB3 decreased the interaction of MYC and UBE3B in the purified system. c Neither the TRIB3 protein nor the KDC domain of TRIB3 affected the interaction of UHCH3 and UBE3B in the purified system. d The interaction of TRIB3 and MYC in Raji cells was evaluated by Co-IP assays. e Colocalization of TRIB3 and MYC in Raji cells was detected with immunostaining (PLA assay). Data are representative images from three independent experiments. Scale bar, 2 μm. f Mapping TRIB3 regions binding to MYC. Top: deletion mutants of TRIB3. Bottom: HEK 293T cells were cotransfected with the indicated constructs of TRIB3 (HA tag) and MYC (Flag tag). Cell extracts were IP with an anti-Flag Ab. g , h Mapping MYC regions binding to TRIB3 ( g ) or UBE3B ( h ). Top: deletion mutants of MYC. Bottom: HEK293T cells were cotransfected with the indicated constructs of MYC and TRIB3 or UBE3B. Cell extracts were IP with an anti-Flag Ab. i TRIB3 overexpression interfered with the interaction of UBE3B and MYC. HEK 293T cells were cotransfected with the indicated constructs. Cell extracts were IP with an anti-MYC Ab. j TRIB3 decreased the binding of UBE3B and MYC. Kinetic interactions of the UBE3B and MYC proteins were determined by SPR analyses with the BSA or TRIB3 proteins. k TRIB3 overexpression increased the interaction of MAX and MYC. HEK 293T cells were cotransfected with the indicated constructs. Cell extracts were IP with an anti-MYC Ab. l TRIB3 overexpression increased the binding of MAX and MYC. Kinetic interactions of the MAX and MYC proteins were determined by SPR analyses with the CTRL (GST) or TRIB3 (TRIB3-GST) protein. m The heterotrimers of MYC, TRIB3, and MAX in Raji cells were detected by Co-IP assays. Cellular extracts were IP with mouse IgG as a negative control or anti‐MYC, anti‐TRIB3, or anti-MAX antibodies. Western blots were performed with anti‐MYC, anti‐TRIB3, and anti-MAX antibodies. n Recovery of the TRIB3 -HA wild-type or KDC -HA mutant enhanced the decreased cell viabilities of TRIB3 Cas9 Jurkat cells for the indicated times. The colors represent different time points; the diameter indicates the relative cell viability. Source data are provided as a Source Data file.
Techniques Used: In Vitro, Purification, Co-Immunoprecipitation Assay, Immunostaining, Proximity Ligation Assay, Binding Assay, Construct, FLAG-tag, Over Expression, SPR Assay, Negative Control, Western Blot, Mutagenesis
Related Articles
Plasmid Preparation:Article Title: TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation Article Snippet: .. Plasmid construction The Human MYC -Flag-tagged (HG11346-CF), SKP2 -Flag-tagged(HG15079-CF), TRIM6 -untagged (HG18626-UT), TRIM32 -untagged (HG18626-UT), TRIM21 -Flag-tagged (HG18010-CF), FBXW7 -His-tagged (HG29625-CH), AURORA -Myc-tagged (HG10669-CM), MAX- His-tagged (HG12885-CH), COP1 -FLAG-tagged (HG19467-CF), TRIB2 -FLAG-tagged (HG10725-CF), UBCH1 (UBE2K) -Myc-tagged (HG16296-CM), UBCH3 (CDC34) -Myc-tagged (HG11443-CM), |