human grb  (Sino Biological)


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    Name:
    Granzyme B cDNA ORF Clone in Cloning Vector Human
    Description:
    Full length Clone DNA of Human granzyme B granzyme 2 cytotoxic T lymphocyte associated serine esterase 1
    Catalog Number:
    hg10345-m
    Product Aliases:
    CCPI cDNA ORF Clone Human, CGL-1 cDNA ORF Clone Human, CGL1 cDNA ORF Clone Human, CSP-B cDNA ORF Clone Human, CSPB cDNA ORF Clone Human, CTLA1 cDNA ORF Clone Human, CTSGL1 cDNA ORF Clone Human, HLP cDNA ORF Clone Human, SECT cDNA ORF Clone Human
    Price:
    75.0
    Size:
    1Unit
    Category:
    cDNA Clone
    Molecule Name:
    GZMB,GZB,Granzyme B,
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    Structured Review

    Sino Biological human grb
    Ectopic <t>GrB</t> upmodulates EMT in CRC cells. GrB negative (SW480, SW620, LoVo) and GrB positive (HT-29) CRC cells were transfected with human GrB vector (pCMV-GrB); Mock-transfected cells were used as control. a GrB <t>transfection</t> efficacy was verified by WB; EMT was investigated analyzing the expression of 3 EMT biomarkers (Snail 1, and E- and N-cadherin) by WB; β-actin was used as loading control; numbers indicate band intensities (b.i.) = band volume/area x mean pixel intensity, normalized for β-actin and quantified using Quantity One 1-D analysis software. b Invasion assay was performed using cells transfected with GrB (pCMV-GrB) or Mock; * p
    Full length Clone DNA of Human granzyme B granzyme 2 cytotoxic T lymphocyte associated serine esterase 1
    https://www.bioz.com/result/human grb/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human grb - by Bioz Stars, 2021-02
    88/100 stars

    Images

    1) Product Images from "Epitelial-to-mesenchimal transition and invasion are upmodulated by tumor-expressed granzyme B and inhibited by docosahexaenoic acid in human colorectal cancer cells"

    Article Title: Epitelial-to-mesenchimal transition and invasion are upmodulated by tumor-expressed granzyme B and inhibited by docosahexaenoic acid in human colorectal cancer cells

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    doi: 10.1186/s13046-016-0302-6

    Ectopic GrB upmodulates EMT in CRC cells. GrB negative (SW480, SW620, LoVo) and GrB positive (HT-29) CRC cells were transfected with human GrB vector (pCMV-GrB); Mock-transfected cells were used as control. a GrB transfection efficacy was verified by WB; EMT was investigated analyzing the expression of 3 EMT biomarkers (Snail 1, and E- and N-cadherin) by WB; β-actin was used as loading control; numbers indicate band intensities (b.i.) = band volume/area x mean pixel intensity, normalized for β-actin and quantified using Quantity One 1-D analysis software. b Invasion assay was performed using cells transfected with GrB (pCMV-GrB) or Mock; * p
    Figure Legend Snippet: Ectopic GrB upmodulates EMT in CRC cells. GrB negative (SW480, SW620, LoVo) and GrB positive (HT-29) CRC cells were transfected with human GrB vector (pCMV-GrB); Mock-transfected cells were used as control. a GrB transfection efficacy was verified by WB; EMT was investigated analyzing the expression of 3 EMT biomarkers (Snail 1, and E- and N-cadherin) by WB; β-actin was used as loading control; numbers indicate band intensities (b.i.) = band volume/area x mean pixel intensity, normalized for β-actin and quantified using Quantity One 1-D analysis software. b Invasion assay was performed using cells transfected with GrB (pCMV-GrB) or Mock; * p

    Techniques Used: Transfection, Plasmid Preparation, Western Blot, Expressing, Software, Invasion Assay

    Related Articles

    Transfection:

    Article Title: Epitelial-to-mesenchimal transition and invasion are upmodulated by tumor-expressed granzyme B and inhibited by docosahexaenoic acid in human colorectal cancer cells
    Article Snippet: .. GrB plasmid transfectionVector for human GrB (Homo sapiens GranzymeB cDNA Clone, Sino Biological Inc, Beijing, China) and Mock were transfected into 70–80 % confluent cells in Opti-MEM (Gibco, Invitrogen), using Lipofectamine LTX and PLUS reagents (Invitrogen). .. Western blottingCell lysates were prepared by a solution containing 50 mM Tris–HCl pH 7.6, 150 mM NaCl, 0.5 % TRITON X-100, 0.5 % Sodium deoxycolate, 0.1 % SDS and the protease inhibitor mixture “Complete” (Roche Diagnostic GmbH, Germany).

    Plasmid Preparation:

    Article Title: Epitelial-to-mesenchimal transition and invasion are upmodulated by tumor-expressed granzyme B and inhibited by docosahexaenoic acid in human colorectal cancer cells
    Article Snippet: .. GrB plasmid transfectionVector for human GrB (Homo sapiens GranzymeB cDNA Clone, Sino Biological Inc, Beijing, China) and Mock were transfected into 70–80 % confluent cells in Opti-MEM (Gibco, Invitrogen), using Lipofectamine LTX and PLUS reagents (Invitrogen). .. Western blottingCell lysates were prepared by a solution containing 50 mM Tris–HCl pH 7.6, 150 mM NaCl, 0.5 % TRITON X-100, 0.5 % Sodium deoxycolate, 0.1 % SDS and the protease inhibitor mixture “Complete” (Roche Diagnostic GmbH, Germany).

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  • 88
    Sino Biological human grb
    Ectopic <t>GrB</t> upmodulates EMT in CRC cells. GrB negative (SW480, SW620, LoVo) and GrB positive (HT-29) CRC cells were transfected with human GrB vector (pCMV-GrB); Mock-transfected cells were used as control. a GrB <t>transfection</t> efficacy was verified by WB; EMT was investigated analyzing the expression of 3 EMT biomarkers (Snail 1, and E- and N-cadherin) by WB; β-actin was used as loading control; numbers indicate band intensities (b.i.) = band volume/area x mean pixel intensity, normalized for β-actin and quantified using Quantity One 1-D analysis software. b Invasion assay was performed using cells transfected with GrB (pCMV-GrB) or Mock; * p
    Human Grb, supplied by Sino Biological, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human grb/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    human grb - by Bioz Stars, 2021-02
    88/100 stars
      Buy from Supplier

    86
    Sino Biological gene expressing tim 3
    Ectopic <t>GrB</t> upmodulates EMT in CRC cells. GrB negative (SW480, SW620, LoVo) and GrB positive (HT-29) CRC cells were transfected with human GrB vector (pCMV-GrB); Mock-transfected cells were used as control. a GrB <t>transfection</t> efficacy was verified by WB; EMT was investigated analyzing the expression of 3 EMT biomarkers (Snail 1, and E- and N-cadherin) by WB; β-actin was used as loading control; numbers indicate band intensities (b.i.) = band volume/area x mean pixel intensity, normalized for β-actin and quantified using Quantity One 1-D analysis software. b Invasion assay was performed using cells transfected with GrB (pCMV-GrB) or Mock; * p
    Gene Expressing Tim 3, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene expressing tim 3/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gene expressing tim 3 - by Bioz Stars, 2021-02
    86/100 stars
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    N/A
    Full length Clone DNA of Human apolipoprotein B mRNA editing enzyme catalytic polypeptide like 2 APOBEC2 with N terminal His tag
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    Image Search Results


    Ectopic GrB upmodulates EMT in CRC cells. GrB negative (SW480, SW620, LoVo) and GrB positive (HT-29) CRC cells were transfected with human GrB vector (pCMV-GrB); Mock-transfected cells were used as control. a GrB transfection efficacy was verified by WB; EMT was investigated analyzing the expression of 3 EMT biomarkers (Snail 1, and E- and N-cadherin) by WB; β-actin was used as loading control; numbers indicate band intensities (b.i.) = band volume/area x mean pixel intensity, normalized for β-actin and quantified using Quantity One 1-D analysis software. b Invasion assay was performed using cells transfected with GrB (pCMV-GrB) or Mock; * p

    Journal: Journal of Experimental & Clinical Cancer Research : CR

    Article Title: Epitelial-to-mesenchimal transition and invasion are upmodulated by tumor-expressed granzyme B and inhibited by docosahexaenoic acid in human colorectal cancer cells

    doi: 10.1186/s13046-016-0302-6

    Figure Lengend Snippet: Ectopic GrB upmodulates EMT in CRC cells. GrB negative (SW480, SW620, LoVo) and GrB positive (HT-29) CRC cells were transfected with human GrB vector (pCMV-GrB); Mock-transfected cells were used as control. a GrB transfection efficacy was verified by WB; EMT was investigated analyzing the expression of 3 EMT biomarkers (Snail 1, and E- and N-cadherin) by WB; β-actin was used as loading control; numbers indicate band intensities (b.i.) = band volume/area x mean pixel intensity, normalized for β-actin and quantified using Quantity One 1-D analysis software. b Invasion assay was performed using cells transfected with GrB (pCMV-GrB) or Mock; * p

    Article Snippet: GrB plasmid transfection Vector for human GrB (Homo sapiens GranzymeB cDNA Clone, Sino Biological Inc, Beijing, China) and Mock were transfected into 70–80 % confluent cells in Opti-MEM (Gibco, Invitrogen), using Lipofectamine LTX and PLUS reagents (Invitrogen).

    Techniques: Transfection, Plasmid Preparation, Western Blot, Expressing, Software, Invasion Assay