isoamp ii universal thda kit  (New England Biolabs)


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    Name:
    IsoAmp II Universal tHDA Kit
    Description:
    IsoAmp II Universal tHDA Kit 50 rxns
    Catalog Number:
    h0110s
    Price:
    409
    Size:
    50 rxns
    Category:
    DNA Amplification Kits
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    New England Biolabs isoamp ii universal thda kit
    IsoAmp II Universal tHDA Kit
    IsoAmp II Universal tHDA Kit 50 rxns
    https://www.bioz.com/result/isoamp ii universal thda kit/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    isoamp ii universal thda kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Luciferase:

    Article Title: A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes
    Article Snippet: IsoAmp II universal tHDA kit was obtained from New England Biolabs Inc. (Ipswich, MA, USA). .. Luciferase assay substrate and pGL6-TA Luciferase reporter which encodes firefly luciferase driven by a minimal TA promoter were purchased from Beyotime Institute of biotechnology, Jiangsu, China.

    Synthesized:

    Article Title: Improved Analytical Sensitivity of Lateral Flow Assay using Sponge for HBV Nucleic Acid Detection
    Article Snippet: HBV probes were synthesized from Sangon Biotechnology Co., Ltd. (Shanghai, China). .. IsoAmp®Ш Universal tHDA Kit was bought from New England Biolabs (NEB, USA).

    Article Title: A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification
    Article Snippet: The TaqMan probe was synthesized by the Biosearch Inc. All oligonucleotides sequences were listed in . .. Real time HDA was performed using the IsoAmp® II Universal tHDA Kit (New England Biolab).

    Construct:

    Article Title: Ribozyme-catalysed RNA synthesis using triplet building blocks
    Article Snippet: Bead-bound constructs were now reverse-transcribed using 1 μM RTri (or HDVRec for the reselection) by resuspension in a Superscript III reaction with added 0.02% Tween-20 (50˚C 30 min). .. Beads were BB washed and the RNA-bound cDNA 3’ end blocked by incubation with terminal deoxynucleotidyl transferase (ThermoFisher) and 0.2 mM dideoxy-ATP (TriLink) with 0.02% added Tween-20 (37˚C 30 min), and beads were BB washed and transferred again. cDNAs were eluted (10 μl 0.1 M NaOH 0.1% Tween-20 20 min), neutralized and plus strands regenerated with 0.2 μM rescue oligonucleotide in an IsoAmp II universal tHDA kit (NEB) reaction (65˚C 60 min) to read through the structured product region.

    SYBR Green Assay:

    Article Title: A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification
    Article Snippet: For real time PCR, QuantiTect SYBR Green PCR Kits (Qiagen Corp.) was used. .. Real time HDA was performed using the IsoAmp® II Universal tHDA Kit (New England Biolab).

    Article Title: A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes
    Article Snippet: IsoAmp II universal tHDA kit was obtained from New England Biolabs Inc. (Ipswich, MA, USA). .. SYBR Green qPCR Mix was obtained from AidLab Biotech, Beijing, China.

    Article Title: A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes
    Article Snippet: A 95 bp GAPDH fragment was amplified using an IsoAmp® II Universal tHDA Kit (New England Biolabs Inc., Ipswich, MA, USA) and specific primers (Table ) following manufacture's two-step reverse transcription-HDA protocol. .. Following incubation for 120 min at 65°C on a VeritiTM 96 well Thermal cycler (Applied Biosystems, Foster City, CA, USA), the products were separated by 2% gel staining with SYBR green and the bands were quantified by ImageLab software (Bio-Rad Laboratories, Berkeley, CA).

    Microarray:

    Article Title: Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices
    Article Snippet: It combines HDA with a microarray-based detection, making it suitable for multiplex detection. .. In this case, the BNI-1 fragment of SARS cDNA was successfully amplified in a real-time format at 62 °C by using the IsoAmp tHDA kit (New England Biolabs, Beverly, MA, USA) containing thermostable UvrD helicase (Tte-UvrD) and thermostable DNA polymerase.

    Incubation:

    Article Title: Ribozyme-catalysed RNA synthesis using triplet building blocks
    Article Snippet: .. Beads were BB washed and the RNA-bound cDNA 3’ end blocked by incubation with terminal deoxynucleotidyl transferase (ThermoFisher) and 0.2 mM dideoxy-ATP (TriLink) with 0.02% added Tween-20 (37˚C 30 min), and beads were BB washed and transferred again. cDNAs were eluted (10 μl 0.1 M NaOH 0.1% Tween-20 20 min), neutralized and plus strands regenerated with 0.2 μM rescue oligonucleotide in an IsoAmp II universal tHDA kit (NEB) reaction (65˚C 60 min) to read through the structured product region. ..

    Article Title: A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes
    Article Snippet: A 95 bp GAPDH fragment was amplified using an IsoAmp® II Universal tHDA Kit (New England Biolabs Inc., Ipswich, MA, USA) and specific primers (Table ) following manufacture's two-step reverse transcription-HDA protocol. .. Briefly, 0.2 μg cDNA template was incubated with annealing buffer and primers for 2 min at 95°C, then the above mix was cooled on ice and mixed with the enzymes mix containing ThermoScript reverse transcriptase, IsoAmp® dNTP and enzymes in annealing buffer.

    Article Title: A Complementary Isothermal Amplification Method to the U.S. EPA Quantitative Polymerase Chain Reaction Approach for the Detection of Enterococci in Environmental Waters
    Article Snippet: Enterococcus HDA Assay Protocol Enterococcus HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England BioLabs, Ipswich, MA) in a final reaction volume of 20 μL. .. Reaction mixtures were then incubated at 65 °C for 90 min. HDA products (10 μL aliquots) were analyzed in 2.5% agarose gels stained with SYBR Gold (Thermo Fisher Scientific, Waltham, MA).

    Amplification:

    Article Title: Advanced Yellow Fever Virus Genome Detection in Point-of-Care Facilities and Reference Laboratories
    Article Snippet: The RT-tHDA assay was optimized using a BioHelix IsoAmp II Universal RT-tHDA kit (New England BioLabs, Frankfurt am Main, Germany). .. In the RT-tHDA assay, excess reverse primer is used to preferentially generate biotin-labeled single-stranded amplicon that can be hybridized in the lateral-flow system.

    Article Title: A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification
    Article Snippet: Paragraph title: Nucleic acid amplification reaction ... Real time HDA was performed using the IsoAmp® II Universal tHDA Kit (New England Biolab).

    Article Title: A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes
    Article Snippet: .. A 95 bp GAPDH fragment was amplified using an IsoAmp® II Universal tHDA Kit (New England Biolabs Inc., Ipswich, MA, USA) and specific primers (Table ) following manufacture's two-step reverse transcription-HDA protocol. .. Briefly, 0.2 μg cDNA template was incubated with annealing buffer and primers for 2 min at 95°C, then the above mix was cooled on ice and mixed with the enzymes mix containing ThermoScript reverse transcriptase, IsoAmp® dNTP and enzymes in annealing buffer.

    Article Title: Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices
    Article Snippet: .. In this case, the BNI-1 fragment of SARS cDNA was successfully amplified in a real-time format at 62 °C by using the IsoAmp tHDA kit (New England Biolabs, Beverly, MA, USA) containing thermostable UvrD helicase (Tte-UvrD) and thermostable DNA polymerase. .. As a consequence of the limited speed and processivity of the UvrD helicase, the system was able to amplify only short DNA sequence (from 70 to 120 bp) [ ].

    Flow Cytometry:

    Article Title: Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices
    Article Snippet: Ramalingam et al. [ ] described a microfluidic devices with an array of open (unsealed) reactors preloaded with primers in conjunction with a single-step capillary-based flow scheme for sequential distribution of the amplification mixture and HDA isothermal amplification of nucleic-acid. .. In this case, the BNI-1 fragment of SARS cDNA was successfully amplified in a real-time format at 62 °C by using the IsoAmp tHDA kit (New England Biolabs, Beverly, MA, USA) containing thermostable UvrD helicase (Tte-UvrD) and thermostable DNA polymerase.

    Activation Assay:

    Article Title: A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification
    Article Snippet: The thermal cycling protocol was as follows: 15 min enzyme activation and predenaturation at 95 °C, 35 cycles of denaturation (95°C for 30 s), annealing (57 °C for 30 s) and elongation (72 °C for 30 s), followed by a final elongation step for 5 min at 72 °C. .. Real time HDA was performed using the IsoAmp® II Universal tHDA Kit (New England Biolab).

    Biomarker Assay:

    Article Title: A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification
    Article Snippet: Rsf-1 biomarker detection was performed with both real time PCR and HDA. .. Real time HDA was performed using the IsoAmp® II Universal tHDA Kit (New England Biolab).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Advanced Yellow Fever Virus Genome Detection in Point-of-Care Facilities and Reference Laboratories
    Article Snippet: Paragraph title: One-step helicase-dependent isothermal RT-PCR (RT-tHDA). ... The RT-tHDA assay was optimized using a BioHelix IsoAmp II Universal RT-tHDA kit (New England BioLabs, Frankfurt am Main, Germany).

    Polymerase Chain Reaction:

    Article Title: Ribozyme-catalysed RNA synthesis using triplet building blocks
    Article Snippet: Beads were BB washed and the RNA-bound cDNA 3’ end blocked by incubation with terminal deoxynucleotidyl transferase (ThermoFisher) and 0.2 mM dideoxy-ATP (TriLink) with 0.02% added Tween-20 (37˚C 30 min), and beads were BB washed and transferred again. cDNAs were eluted (10 μl 0.1 M NaOH 0.1% Tween-20 20 min), neutralized and plus strands regenerated with 0.2 μM rescue oligonucleotide in an IsoAmp II universal tHDA kit (NEB) reaction (65˚C 60 min) to read through the structured product region. .. Each 50 μg of beads were then subjected to plus strand recovery PCR in a 100 μl GoTaq HotStart reaction with 0.5 μM each RTri (or HDVrec for reselection) and RecInt (rounds 1–5)/RecIntQ (rounds 6–9)/RecIntL (rounds 10–14, 19–21 and reselection)/RecIntQL (rounds 15–18).

    Article Title: A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification
    Article Snippet: For real time PCR, QuantiTect SYBR Green PCR Kits (Qiagen Corp.) was used. .. Real time HDA was performed using the IsoAmp® II Universal tHDA Kit (New England Biolab).

    Binding Assay:

    Article Title: Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices
    Article Snippet: In this case, the BNI-1 fragment of SARS cDNA was successfully amplified in a real-time format at 62 °C by using the IsoAmp tHDA kit (New England Biolabs, Beverly, MA, USA) containing thermostable UvrD helicase (Tte-UvrD) and thermostable DNA polymerase. .. It has been reported that the speed and the robustness of HDA are highly dependent on the synchronization of helicase, single-stranded DNA binding protein and large fragment of Bst DNA polymerase [ ].

    MTT Assay:

    Article Title: A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes
    Article Snippet: IsoAmp II universal tHDA kit was obtained from New England Biolabs Inc. (Ipswich, MA, USA). .. Calf thymus DNA (CT DNA), 3-(4,5- dimethylthiazoyl-2-yl) 2,5-diphenyltetrazolium bromide (MTT), propidium iodide (PI), ethidium bromide (EB) and Hoechst33258 were obtained from Sigma-Aldrich (St. Louis, MO).

    Sequencing:

    Article Title: Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices
    Article Snippet: In this case, the BNI-1 fragment of SARS cDNA was successfully amplified in a real-time format at 62 °C by using the IsoAmp tHDA kit (New England Biolabs, Beverly, MA, USA) containing thermostable UvrD helicase (Tte-UvrD) and thermostable DNA polymerase. .. As a consequence of the limited speed and processivity of the UvrD helicase, the system was able to amplify only short DNA sequence (from 70 to 120 bp) [ ].

    Blocking Assay:

    Article Title: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test
    Article Snippet: .. BacR HDA-strip test assay protocol BacR HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England Biolabs, Ipswich, MA) in a final reaction volume of 20 µL. ..

    Article Title: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test
    Article Snippet: .. BacR HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England Biolabs, Ipswich, MA) in a final reaction volume of 20 µL. ..

    Article Title: A Complementary Isothermal Amplification Method to the U.S. EPA Quantitative Polymerase Chain Reaction Approach for the Detection of Enterococci in Environmental Waters
    Article Snippet: .. Enterococcus HDA Assay Protocol Enterococcus HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England BioLabs, Ipswich, MA) in a final reaction volume of 20 μL. ..

    Helicase-dependent Amplification:

    Article Title: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test
    Article Snippet: .. BacR HDA-strip test assay protocol BacR HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England Biolabs, Ipswich, MA) in a final reaction volume of 20 µL. ..

    Article Title: A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification
    Article Snippet: .. Real time HDA was performed using the IsoAmp® II Universal tHDA Kit (New England Biolab). .. The real time HDA mixture was prepared according to the manufacturer’s instructions except the primer concentration was 100 nM each and EvaGreen was used at 1× concentration.

    Article Title: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test
    Article Snippet: .. BacR HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England Biolabs, Ipswich, MA) in a final reaction volume of 20 µL. ..

    Article Title: A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes
    Article Snippet: Paragraph title: Thermophilic helicase-dependent amplification (tHDA) ... A 95 bp GAPDH fragment was amplified using an IsoAmp® II Universal tHDA Kit (New England Biolabs Inc., Ipswich, MA, USA) and specific primers (Table ) following manufacture's two-step reverse transcription-HDA protocol.

    Article Title: Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices
    Article Snippet: Paragraph title: 3.2. Helicase-Dependent Amplification (HDA) ... In this case, the BNI-1 fragment of SARS cDNA was successfully amplified in a real-time format at 62 °C by using the IsoAmp tHDA kit (New England Biolabs, Beverly, MA, USA) containing thermostable UvrD helicase (Tte-UvrD) and thermostable DNA polymerase.

    Article Title: A Complementary Isothermal Amplification Method to the U.S. EPA Quantitative Polymerase Chain Reaction Approach for the Detection of Enterococci in Environmental Waters
    Article Snippet: .. Enterococcus HDA Assay Protocol Enterococcus HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England BioLabs, Ipswich, MA) in a final reaction volume of 20 μL. ..

    Software:

    Article Title: A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes
    Article Snippet: A 95 bp GAPDH fragment was amplified using an IsoAmp® II Universal tHDA Kit (New England Biolabs Inc., Ipswich, MA, USA) and specific primers (Table ) following manufacture's two-step reverse transcription-HDA protocol. .. Following incubation for 120 min at 65°C on a VeritiTM 96 well Thermal cycler (Applied Biosystems, Foster City, CA, USA), the products were separated by 2% gel staining with SYBR green and the bands were quantified by ImageLab software (Bio-Rad Laboratories, Berkeley, CA).

    Real-time Polymerase Chain Reaction:

    Article Title: A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification
    Article Snippet: The real time PCR mixture contained 1×SYBR Green master mix and primers (forward primer and reverse primer each at 400 nM). .. Real time HDA was performed using the IsoAmp® II Universal tHDA Kit (New England Biolab).

    Article Title: A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes
    Article Snippet: IsoAmp II universal tHDA kit was obtained from New England Biolabs Inc. (Ipswich, MA, USA). .. SYBR Green qPCR Mix was obtained from AidLab Biotech, Beijing, China.

    Multiplex Assay:

    Article Title: Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices
    Article Snippet: It combines HDA with a microarray-based detection, making it suitable for multiplex detection. .. In this case, the BNI-1 fragment of SARS cDNA was successfully amplified in a real-time format at 62 °C by using the IsoAmp tHDA kit (New England Biolabs, Beverly, MA, USA) containing thermostable UvrD helicase (Tte-UvrD) and thermostable DNA polymerase.

    In Vitro:

    Article Title: Ribozyme-catalysed RNA synthesis using triplet building blocks
    Article Snippet: Paragraph title: In vitro evolution cycle ... Beads were BB washed and the RNA-bound cDNA 3’ end blocked by incubation with terminal deoxynucleotidyl transferase (ThermoFisher) and 0.2 mM dideoxy-ATP (TriLink) with 0.02% added Tween-20 (37˚C 30 min), and beads were BB washed and transferred again. cDNAs were eluted (10 μl 0.1 M NaOH 0.1% Tween-20 20 min), neutralized and plus strands regenerated with 0.2 μM rescue oligonucleotide in an IsoAmp II universal tHDA kit (NEB) reaction (65˚C 60 min) to read through the structured product region.

    Concentration Assay:

    Article Title: A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification
    Article Snippet: Real time HDA was performed using the IsoAmp® II Universal tHDA Kit (New England Biolab). .. The real time HDA mixture was prepared according to the manufacturer’s instructions except the primer concentration was 100 nM each and EvaGreen was used at 1× concentration.

    Article Title: Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices
    Article Snippet: In this case, the BNI-1 fragment of SARS cDNA was successfully amplified in a real-time format at 62 °C by using the IsoAmp tHDA kit (New England Biolabs, Beverly, MA, USA) containing thermostable UvrD helicase (Tte-UvrD) and thermostable DNA polymerase. .. By increasing the concentration of the constituent enzymes, while maintaining the relative proportions of each type of enzyme constant, the overall reaction speed has been increased.

    Marker:

    Article Title: Ribozyme-catalysed RNA synthesis using triplet building blocks
    Article Snippet: The marker-adjacent gel region in the construct lane was excised, excluding the bulk unreacted construct (judged by UV shadowing). .. Beads were BB washed and the RNA-bound cDNA 3’ end blocked by incubation with terminal deoxynucleotidyl transferase (ThermoFisher) and 0.2 mM dideoxy-ATP (TriLink) with 0.02% added Tween-20 (37˚C 30 min), and beads were BB washed and transferred again. cDNAs were eluted (10 μl 0.1 M NaOH 0.1% Tween-20 20 min), neutralized and plus strands regenerated with 0.2 μM rescue oligonucleotide in an IsoAmp II universal tHDA kit (NEB) reaction (65˚C 60 min) to read through the structured product region.

    Staining:

    Article Title: A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes
    Article Snippet: A 95 bp GAPDH fragment was amplified using an IsoAmp® II Universal tHDA Kit (New England Biolabs Inc., Ipswich, MA, USA) and specific primers (Table ) following manufacture's two-step reverse transcription-HDA protocol. .. Following incubation for 120 min at 65°C on a VeritiTM 96 well Thermal cycler (Applied Biosystems, Foster City, CA, USA), the products were separated by 2% gel staining with SYBR green and the bands were quantified by ImageLab software (Bio-Rad Laboratories, Berkeley, CA).

    Article Title: A Complementary Isothermal Amplification Method to the U.S. EPA Quantitative Polymerase Chain Reaction Approach for the Detection of Enterococci in Environmental Waters
    Article Snippet: Enterococcus HDA Assay Protocol Enterococcus HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England BioLabs, Ipswich, MA) in a final reaction volume of 20 μL. .. Reaction mixtures were then incubated at 65 °C for 90 min. HDA products (10 μL aliquots) were analyzed in 2.5% agarose gels stained with SYBR Gold (Thermo Fisher Scientific, Waltham, MA).

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  • 95
    New England Biolabs isoamp ii universal thda kit
    Isoamp Ii Universal Thda Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoamp ii universal thda kit/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    isoamp ii universal thda kit - by Bioz Stars, 2020-02
    95/100 stars
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