isoamp thda kit  (New England Biolabs)


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  • 94
    Name:
    IsoAmp II Universal tHDA Kit
    Description:
    IsoAmp II Universal tHDA Kit 50 rxns
    Catalog Number:
    H0110S
    Price:
    409
    Category:
    DNA Amplification Kits
    Size:
    50 rxns
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    Structured Review

    New England Biolabs isoamp thda kit
    IsoAmp II Universal tHDA Kit
    IsoAmp II Universal tHDA Kit 50 rxns
    https://www.bioz.com/result/isoamp thda kit/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isoamp thda kit - by Bioz Stars, 2021-06
    94/100 stars

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    Related Articles

    Amplification:

    Article Title: A novel triazolonaphthalimide induces apoptosis and inhibits tumor growth by targeting DNA and DNA-associated processes
    Article Snippet: Thermophilic helicase-dependent amplification (tHDA) Isothermal helicase-dependent amplification (HDA) assay was further performed to evaluate the impact of LSS-11 on DNA replication efficiency without thermal cycling. .. A 95 bp GAPDH fragment was amplified using an IsoAmp® II Universal tHDA Kit (New England Biolabs Inc., Ipswich, MA, USA) and specific primers (Table ) following manufacture's two-step reverse transcription-HDA protocol. .. Briefly, 0.2 μg cDNA template was incubated with annealing buffer and primers for 2 min at 95°C, then the above mix was cooled on ice and mixed with the enzymes mix containing ThermoScript reverse transcriptase, IsoAmp® dNTP and enzymes in annealing buffer.

    Article Title: Isothermal Amplification Methods for the Detection of Nucleic Acids in Microfluidic Devices
    Article Snippet: Ramalingam et al. [ ] described a microfluidic devices with an array of open (unsealed) reactors preloaded with primers in conjunction with a single-step capillary-based flow scheme for sequential distribution of the amplification mixture and HDA isothermal amplification of nucleic-acid. .. In this case, the BNI-1 fragment of SARS cDNA was successfully amplified in a real-time format at 62 °C by using the IsoAmp tHDA kit (New England Biolabs, Beverly, MA, USA) containing thermostable UvrD helicase (Tte-UvrD) and thermostable DNA polymerase. .. As a consequence of the limited speed and processivity of the UvrD helicase, the system was able to amplify only short DNA sequence (from 70 to 120 bp) [ ].

    Helicase-dependent Amplification:

    Article Title: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test
    Article Snippet: .. BacR HDA-strip test assay protocol BacR HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England Biolabs, Ipswich, MA) in a final reaction volume of 20 µL. ..

    Article Title: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test
    Article Snippet: .. BacR HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England Biolabs, Ipswich, MA) in a final reaction volume of 20 µL. ..

    Article Title: LAMP-based foldable microdevice platform for the rapid detection of Magnaporthe oryzae and Sarocladium oryzae in rice seed
    Article Snippet: The assay results were analyzed by plotting the log of the template DNA concentration against cycle threshold (C t) values. .. The HDA assay The HDA assay was performed using the IsoAmp II universal tHDA Kit (BioLab Inc., New England Biologicals, USA). ..

    Article Title: A surface topography assisted droplet manipulation platform for biomarker detection and pathogen identification
    Article Snippet: The thermal cycling protocol was as follows: 15 min enzyme activation and predenaturation at 95 °C, 35 cycles of denaturation (95°C for 30 s), annealing (57 °C for 30 s) and elongation (72 °C for 30 s), followed by a final elongation step for 5 min at 72 °C. .. Real time HDA was performed using the IsoAmp® II Universal tHDA Kit (New England Biolab). .. The real time HDA mixture was prepared according to the manufacturer’s instructions except the primer concentration was 100 nM each and EvaGreen was used at 1× concentration.

    Blocking Assay:

    Article Title: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test
    Article Snippet: .. BacR HDA-strip test assay protocol BacR HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England Biolabs, Ipswich, MA) in a final reaction volume of 20 µL. ..

    Article Title: Detection of a microbial source tracking marker by isothermal helicase-dependent amplification and a nucleic acid lateral-flow strip test
    Article Snippet: .. BacR HDA reactions were performed on a heating block (PocketBloc, Biozym, Germany), using the IsoAmp II Universal tHDA kit (New England Biolabs, Ipswich, MA) in a final reaction volume of 20 µL. ..

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  • 94
    New England Biolabs isoamp thda kit
    Isoamp Thda Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoamp thda kit/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isoamp thda kit - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

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