golden gate assembly mix  (New England Biolabs)


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    New England Biolabs golden gate assembly mix
    Golden Gate Assembly Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bsmbi v2 golden gate assembly  (New England Biolabs)


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    New England Biolabs bsmbi v2 golden gate assembly
    Bsmbi V2 Golden Gate Assembly, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bsmbi v2 golden gate assembly kit  (New England Biolabs)


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    New England Biolabs bsmbi v2 golden gate assembly kit
    Bsmbi V2 Golden Gate Assembly Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nebridge golden gate assembly kit bsmbi v2  (New England Biolabs)


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    New England Biolabs nebridge golden gate assembly kit bsmbi v2
    Nebridge Golden Gate Assembly Kit Bsmbi V2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    golden gate assembly mix  (New England Biolabs)


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    New England Biolabs golden gate assembly mix
    Golden Gate Assembly Mix, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    esp3i bsmbi restriction enzyme  (New England Biolabs)


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    New England Biolabs esp3i bsmbi restriction enzyme
    ( A ) The low-copy plasmid pGal-BE3 encodes the base editor v3 (BE3; ) under a galactose-inducible promoter ( GALL ). This plasmid also encodes LEU2 for selection. ( B ) The high-copy pgRNA-backbone plasmid encodes a 2 kb placeholder flanked by the SNR52 promoter (a Polymerase-III promoter) and SUP4 terminator. The placeholder can be removed by <t>Esp3I/BsmBI</t> restriction digest and then a gRNA can be cloned in either by Golden Gate Assembly or by Gibson Assembly. This plasmid also encodes the URA3 gene for selection. ( C ) This plasmid is identical to the one shown in (B) but with a barcode cassette added. The barcode cassette contains a Kanamycin resistance gene. ( D ) Sequences at the gRNA cloning site of the pgRNA-backbone plasmid to facilitate gRNA plasmid cloning (individually by Golden Gate Assembly or as a library by Gibson Assembly). Rows 3 and 4 show the two types of DNA fragments containing the gRNA that can be cloned into the digested backbone plasmid.
    Esp3i Bsmbi Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Genome-wide base editor screen identifies regulators of protein abundance in yeast"

    Article Title: Genome-wide base editor screen identifies regulators of protein abundance in yeast

    Journal: eLife

    doi: 10.7554/eLife.79525

    ( A ) The low-copy plasmid pGal-BE3 encodes the base editor v3 (BE3; ) under a galactose-inducible promoter ( GALL ). This plasmid also encodes LEU2 for selection. ( B ) The high-copy pgRNA-backbone plasmid encodes a 2 kb placeholder flanked by the SNR52 promoter (a Polymerase-III promoter) and SUP4 terminator. The placeholder can be removed by Esp3I/BsmBI restriction digest and then a gRNA can be cloned in either by Golden Gate Assembly or by Gibson Assembly. This plasmid also encodes the URA3 gene for selection. ( C ) This plasmid is identical to the one shown in (B) but with a barcode cassette added. The barcode cassette contains a Kanamycin resistance gene. ( D ) Sequences at the gRNA cloning site of the pgRNA-backbone plasmid to facilitate gRNA plasmid cloning (individually by Golden Gate Assembly or as a library by Gibson Assembly). Rows 3 and 4 show the two types of DNA fragments containing the gRNA that can be cloned into the digested backbone plasmid.
    Figure Legend Snippet: ( A ) The low-copy plasmid pGal-BE3 encodes the base editor v3 (BE3; ) under a galactose-inducible promoter ( GALL ). This plasmid also encodes LEU2 for selection. ( B ) The high-copy pgRNA-backbone plasmid encodes a 2 kb placeholder flanked by the SNR52 promoter (a Polymerase-III promoter) and SUP4 terminator. The placeholder can be removed by Esp3I/BsmBI restriction digest and then a gRNA can be cloned in either by Golden Gate Assembly or by Gibson Assembly. This plasmid also encodes the URA3 gene for selection. ( C ) This plasmid is identical to the one shown in (B) but with a barcode cassette added. The barcode cassette contains a Kanamycin resistance gene. ( D ) Sequences at the gRNA cloning site of the pgRNA-backbone plasmid to facilitate gRNA plasmid cloning (individually by Golden Gate Assembly or as a library by Gibson Assembly). Rows 3 and 4 show the two types of DNA fragments containing the gRNA that can be cloned into the digested backbone plasmid.

    Techniques Used: Plasmid Preparation, Selection, Clone Assay

    golden gate assembly kit bsmbi v2  (New England Biolabs)


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    New England Biolabs golden gate assembly kit bsmbi v2
    Golden Gate Assembly Kit Bsmbi V2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    nebridge golden gate assembly kit bsai hfv2  (New England Biolabs)


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    New England Biolabs nebridge golden gate assembly kit bsai hfv2
    Nebridge Golden Gate Assembly Kit Bsai Hfv2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bsai hf v2 golden gate enzyme mixture  (New England Biolabs)


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    golden gate assembly kit  (New England Biolabs)


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    New England Biolabs golden gate assembly kit
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    bsmbi v2 golden gate assembly  (New England Biolabs)


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    New England Biolabs bsmbi v2 golden gate assembly
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    New England Biolabs golden gate assembly mix
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    New England Biolabs esp3i bsmbi restriction enzyme
    ( A ) The low-copy plasmid pGal-BE3 encodes the base editor v3 (BE3; ) under a galactose-inducible promoter ( GALL ). This plasmid also encodes LEU2 for selection. ( B ) The high-copy pgRNA-backbone plasmid encodes a 2 kb placeholder flanked by the SNR52 promoter (a Polymerase-III promoter) and SUP4 terminator. The placeholder can be removed by <t>Esp3I/BsmBI</t> restriction digest and then a gRNA can be cloned in either by Golden Gate Assembly or by Gibson Assembly. This plasmid also encodes the URA3 gene for selection. ( C ) This plasmid is identical to the one shown in (B) but with a barcode cassette added. The barcode cassette contains a Kanamycin resistance gene. ( D ) Sequences at the gRNA cloning site of the pgRNA-backbone plasmid to facilitate gRNA plasmid cloning (individually by Golden Gate Assembly or as a library by Gibson Assembly). Rows 3 and 4 show the two types of DNA fragments containing the gRNA that can be cloned into the digested backbone plasmid.
    Esp3i Bsmbi Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) The low-copy plasmid pGal-BE3 encodes the base editor v3 (BE3; ) under a galactose-inducible promoter ( GALL ). This plasmid also encodes LEU2 for selection. ( B ) The high-copy pgRNA-backbone plasmid encodes a 2 kb placeholder flanked by the SNR52 promoter (a Polymerase-III promoter) and SUP4 terminator. The placeholder can be removed by <t>Esp3I/BsmBI</t> restriction digest and then a gRNA can be cloned in either by Golden Gate Assembly or by Gibson Assembly. This plasmid also encodes the URA3 gene for selection. ( C ) This plasmid is identical to the one shown in (B) but with a barcode cassette added. The barcode cassette contains a Kanamycin resistance gene. ( D ) Sequences at the gRNA cloning site of the pgRNA-backbone plasmid to facilitate gRNA plasmid cloning (individually by Golden Gate Assembly or as a library by Gibson Assembly). Rows 3 and 4 show the two types of DNA fragments containing the gRNA that can be cloned into the digested backbone plasmid.
    Golden Gate Assembly Kit Bsmbi V2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/golden gate assembly kit bsmbi v2/product/New England Biolabs
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    New England Biolabs nebridge golden gate assembly kit bsai hfv2
    ( A ) The low-copy plasmid pGal-BE3 encodes the base editor v3 (BE3; ) under a galactose-inducible promoter ( GALL ). This plasmid also encodes LEU2 for selection. ( B ) The high-copy pgRNA-backbone plasmid encodes a 2 kb placeholder flanked by the SNR52 promoter (a Polymerase-III promoter) and SUP4 terminator. The placeholder can be removed by <t>Esp3I/BsmBI</t> restriction digest and then a gRNA can be cloned in either by Golden Gate Assembly or by Gibson Assembly. This plasmid also encodes the URA3 gene for selection. ( C ) This plasmid is identical to the one shown in (B) but with a barcode cassette added. The barcode cassette contains a Kanamycin resistance gene. ( D ) Sequences at the gRNA cloning site of the pgRNA-backbone plasmid to facilitate gRNA plasmid cloning (individually by Golden Gate Assembly or as a library by Gibson Assembly). Rows 3 and 4 show the two types of DNA fragments containing the gRNA that can be cloned into the digested backbone plasmid.
    Nebridge Golden Gate Assembly Kit Bsai Hfv2, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ( A ) The low-copy plasmid pGal-BE3 encodes the base editor v3 (BE3; ) under a galactose-inducible promoter ( GALL ). This plasmid also encodes LEU2 for selection. ( B ) The high-copy pgRNA-backbone plasmid encodes a 2 kb placeholder flanked by the SNR52 promoter (a Polymerase-III promoter) and SUP4 terminator. The placeholder can be removed by <t>Esp3I/BsmBI</t> restriction digest and then a gRNA can be cloned in either by Golden Gate Assembly or by Gibson Assembly. This plasmid also encodes the URA3 gene for selection. ( C ) This plasmid is identical to the one shown in (B) but with a barcode cassette added. The barcode cassette contains a Kanamycin resistance gene. ( D ) Sequences at the gRNA cloning site of the pgRNA-backbone plasmid to facilitate gRNA plasmid cloning (individually by Golden Gate Assembly or as a library by Gibson Assembly). Rows 3 and 4 show the two types of DNA fragments containing the gRNA that can be cloned into the digested backbone plasmid.
    Bsai Hf V2 Golden Gate Enzyme Mixture, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs golden gate assembly kit
    ( A ) The low-copy plasmid pGal-BE3 encodes the base editor v3 (BE3; ) under a galactose-inducible promoter ( GALL ). This plasmid also encodes LEU2 for selection. ( B ) The high-copy pgRNA-backbone plasmid encodes a 2 kb placeholder flanked by the SNR52 promoter (a Polymerase-III promoter) and SUP4 terminator. The placeholder can be removed by <t>Esp3I/BsmBI</t> restriction digest and then a gRNA can be cloned in either by Golden Gate Assembly or by Gibson Assembly. This plasmid also encodes the URA3 gene for selection. ( C ) This plasmid is identical to the one shown in (B) but with a barcode cassette added. The barcode cassette contains a Kanamycin resistance gene. ( D ) Sequences at the gRNA cloning site of the pgRNA-backbone plasmid to facilitate gRNA plasmid cloning (individually by Golden Gate Assembly or as a library by Gibson Assembly). Rows 3 and 4 show the two types of DNA fragments containing the gRNA that can be cloned into the digested backbone plasmid.
    Golden Gate Assembly Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    ( A ) The low-copy plasmid pGal-BE3 encodes the base editor v3 (BE3; ) under a galactose-inducible promoter ( GALL ). This plasmid also encodes LEU2 for selection. ( B ) The high-copy pgRNA-backbone plasmid encodes a 2 kb placeholder flanked by the SNR52 promoter (a Polymerase-III promoter) and SUP4 terminator. The placeholder can be removed by Esp3I/BsmBI restriction digest and then a gRNA can be cloned in either by Golden Gate Assembly or by Gibson Assembly. This plasmid also encodes the URA3 gene for selection. ( C ) This plasmid is identical to the one shown in (B) but with a barcode cassette added. The barcode cassette contains a Kanamycin resistance gene. ( D ) Sequences at the gRNA cloning site of the pgRNA-backbone plasmid to facilitate gRNA plasmid cloning (individually by Golden Gate Assembly or as a library by Gibson Assembly). Rows 3 and 4 show the two types of DNA fragments containing the gRNA that can be cloned into the digested backbone plasmid.

    Journal: eLife

    Article Title: Genome-wide base editor screen identifies regulators of protein abundance in yeast

    doi: 10.7554/eLife.79525

    Figure Lengend Snippet: ( A ) The low-copy plasmid pGal-BE3 encodes the base editor v3 (BE3; ) under a galactose-inducible promoter ( GALL ). This plasmid also encodes LEU2 for selection. ( B ) The high-copy pgRNA-backbone plasmid encodes a 2 kb placeholder flanked by the SNR52 promoter (a Polymerase-III promoter) and SUP4 terminator. The placeholder can be removed by Esp3I/BsmBI restriction digest and then a gRNA can be cloned in either by Golden Gate Assembly or by Gibson Assembly. This plasmid also encodes the URA3 gene for selection. ( C ) This plasmid is identical to the one shown in (B) but with a barcode cassette added. The barcode cassette contains a Kanamycin resistance gene. ( D ) Sequences at the gRNA cloning site of the pgRNA-backbone plasmid to facilitate gRNA plasmid cloning (individually by Golden Gate Assembly or as a library by Gibson Assembly). Rows 3 and 4 show the two types of DNA fragments containing the gRNA that can be cloned into the digested backbone plasmid.

    Article Snippet: The resulting double-stranded DNA fragment with 4-nucleotide overhangs was then inserted into the pgRNA-backbone plasmid by Golden Gate Assembly ( ) using the Esp3I/BsmBI restriction enzyme (NEB #E1602S).

    Techniques: Plasmid Preparation, Selection, Clone Assay