ez rna methylationtm kit  (Zymo Research)


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    Name:
    EZ RNA Methylation Kit
    Description:
    The EZ RNA Methylation Kit features rapid and reliable bisulfite conversion of cytosines in RNA for methylation analysis The kit streamlines the three step process for complete conversion of cytosine in into uracil RNA denaturation and bisulfite conversion processes are combined into a single step No buffer preparation is necessary The RNA Conversion Reagent is provided ready to use simply add the reagent to an RNA sample and incubate as indicated The innovative in column desulphonation technology eliminates messy precipitation steps ensuring researchers obtain consistent results The product has been designed to minimize template degradation loss of RNA during treatment and clean up and to provide complete conversion of cytosine for accurate methylation analysis Recovered RNA is ideal for RT PCR sequencing library preparation and Next Gen sequencing
    Catalog Number:
    r5001
    Price:
    None
    Applications:
    Bisulfite Conversion
    Size:
    50 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research ez rna methylationtm kit
    EZ RNA Methylation Kit
    The EZ RNA Methylation Kit features rapid and reliable bisulfite conversion of cytosines in RNA for methylation analysis The kit streamlines the three step process for complete conversion of cytosine in into uracil RNA denaturation and bisulfite conversion processes are combined into a single step No buffer preparation is necessary The RNA Conversion Reagent is provided ready to use simply add the reagent to an RNA sample and incubate as indicated The innovative in column desulphonation technology eliminates messy precipitation steps ensuring researchers obtain consistent results The product has been designed to minimize template degradation loss of RNA during treatment and clean up and to provide complete conversion of cytosine for accurate methylation analysis Recovered RNA is ideal for RT PCR sequencing library preparation and Next Gen sequencing
    https://www.bioz.com/result/ez rna methylationtm kit/product/Zymo Research
    Average 95 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    ez rna methylationtm kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Methylation Sequencing:

    Article Title: Comprehensive DNA methylation analysis of the Aedes aegypti genome
    Article Snippet: Paragraph title: tRNA bisulfite sequencing ... Bisulfite conversion was performed using the EZ RNA Methylation Kit (Zymo Research).

    Article Title: Dnmt2 mediates intergenerational transmission of paternally acquired metabolic disorders through sperm small non-coding RNAs
    Article Snippet: Paragraph title: RNA bisulfite sequencing ... Bisulfite conversion was performed using the EZ RNA MethylationTM Kit (Zymo Research, catalog number: R5001) according to manufacturer’s protocol.

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: .. RNA bisulfite sequencing Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo. .. Amplicons for 454 (Roche) sequencing were generated by PCR as described in Tuorto et al ( ).

    Article Title: The tRNA methyltransferase Dnmt2 is required for accurate polypeptide synthesis during haematopoiesis
    Article Snippet: .. RNA bisulfite sequencing Bisulfite conversion was performed using the EZ RNA Methylation™ Kit (Zymo Research). .. Amplicons for 454 (Roche) sequencing were generated and analyzed as described previously (Tuorto et al , ).

    Article Title: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan
    Article Snippet: Paragraph title: Bisulfite sequencing ... Alternatively, the EZ RNA Methylation Kit (Zymo Research) was used for bisulfite treatment and purification of converted RNA according to the instructions provided by the manufacturer.

    Article Title: Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs
    Article Snippet: .. 454 Life Sciences (Roche) bisulfite sequencing was performed using the EZ RNA Methylation Kit (Zymo Research). .. PCR primers are provided in .

    Article Title: Long non-coding RNAs as targets for cytosine methylation
    Article Snippet: .. Bisulfite sequencing To establish the BS sequencing protocol, total RNA from E. coli was subjected to either one round of incubation for 5 min at 70°C followed by 90 min at 64°C ( ) or three cycles of alternating incubations of 5 min at 70°C/45 min at 64°C ( ) using the EZ RNA Methylation Kit, Beta Version (Zymo Research) according to the manufacturer’s instructions. ..

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: Paragraph title: RNA bisulfite sequencing ... Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo.

    Cell Isolation:

    Article Title: LXR/ApoE activation restricts innate immune suppression in cancer
    Article Snippet: MDSCs were isolated using the Myeloid-Derived Suppressor Cell Isolation Kit (130-094-538, Miltenyi Biotec). .. RNA was extracted from MDSCs using the Total RNA Extraction Kit (17200, Noragen), then purified and concentrated using the RNA Clean & Concentrator -5 Kit (R1016, Zymo Research).

    Amplification:

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: RNA bisulfite sequencing Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo. .. For each amplicon, sequences showing a conversion > 95% and a lower threshold identity > 90% were aligned and aggregated in heatmaps.

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo. .. For each amplicon, sequences showing a conversion > 95% and a lower threshold identity > 90% were aligned and aggregated in heatmaps.

    Touchdown PCR:

    Article Title: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan
    Article Snippet: Five μl complementary DNA (cDNA) was subjected to the following touchdown-PCR programme in a 25 μl reaction with KAPA2G Robust Polymerase and Buffer A (Kapa Biosystems): PCR products were gel purified using PCR- and Gel Extraction Kit (Favorgen) and eluded in 30 μl. .. Alternatively, the EZ RNA Methylation Kit (Zymo Research) was used for bisulfite treatment and purification of converted RNA according to the instructions provided by the manufacturer.

    Incubation:

    Article Title: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan
    Article Snippet: RNA was desalted using a Micro Bio-Spin 6 SSC column (Biorad), mixed with 80 μl 1 M Tris-Cl (pH 9.0) buffer and incubated at 37 °C for 1 h. After isopropanol precipitation, 400 ng RNA was reverse transcribed using Super Script III reverse transcriptase (Life Technologies) and gene-specific reverse primers ( ) for 50 min following the manufacturer’s instructions. .. Alternatively, the EZ RNA Methylation Kit (Zymo Research) was used for bisulfite treatment and purification of converted RNA according to the instructions provided by the manufacturer.

    Article Title: Long non-coding RNAs as targets for cytosine methylation
    Article Snippet: .. Bisulfite sequencing To establish the BS sequencing protocol, total RNA from E. coli was subjected to either one round of incubation for 5 min at 70°C followed by 90 min at 64°C ( ) or three cycles of alternating incubations of 5 min at 70°C/45 min at 64°C ( ) using the EZ RNA Methylation Kit, Beta Version (Zymo Research) according to the manufacturer’s instructions. ..

    Genome Wide:

    Article Title: Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine
    Article Snippet: Paragraph title: Genome-wide tRNA methylome analysis ... Extracted RNAs were subjected to bisulfite conversion using the EZ RNA Methylation kit (Zymo).

    Cell Culture:

    Article Title: Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs
    Article Snippet: Paragraph title: Cell culture, RNA isolation, and library preparation ... Small and long fractions were DNase-digested and bisulfite converted using the EZ RNA Methylation Kit (Zymo Research).

    Generated:

    Article Title: Comprehensive DNA methylation analysis of the Aedes aegypti genome
    Article Snippet: Bisulfite conversion was performed using the EZ RNA Methylation Kit (Zymo Research). .. Amplicons for 454 (Roche) sequencing were generated and analyzed as described previously .

    Article Title: Dnmt2 mediates intergenerational transmission of paternally acquired metabolic disorders through sperm small non-coding RNAs
    Article Snippet: Bisulfite conversion was performed using the EZ RNA MethylationTM Kit (Zymo Research, catalog number: R5001) according to manufacturer’s protocol. .. Amplicons for 454 (Roche) sequencing were generated and analyzed as described previously .

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: RNA bisulfite sequencing Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo. .. Amplicons for 454 (Roche) sequencing were generated by PCR as described in Tuorto et al ( ).

    Article Title: The tRNA methyltransferase Dnmt2 is required for accurate polypeptide synthesis during haematopoiesis
    Article Snippet: RNA bisulfite sequencing Bisulfite conversion was performed using the EZ RNA Methylation™ Kit (Zymo Research). .. Amplicons for 454 (Roche) sequencing were generated and analyzed as described previously (Tuorto et al , ).

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo. .. Amplicons for 454 (Roche) sequencing were generated by PCR as described in Tuorto et al ( ).

    Sequencing:

    Article Title: Comprehensive DNA methylation analysis of the Aedes aegypti genome
    Article Snippet: Bisulfite conversion was performed using the EZ RNA Methylation Kit (Zymo Research). .. Amplicons for 454 (Roche) sequencing were generated and analyzed as described previously .

    Article Title: Dnmt2 mediates intergenerational transmission of paternally acquired metabolic disorders through sperm small non-coding RNAs
    Article Snippet: Bisulfite conversion was performed using the EZ RNA MethylationTM Kit (Zymo Research, catalog number: R5001) according to manufacturer’s protocol. .. Amplicons for 454 (Roche) sequencing were generated and analyzed as described previously .

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: RNA bisulfite sequencing Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo. .. Amplicons for 454 (Roche) sequencing were generated by PCR as described in Tuorto et al ( ).

    Article Title: Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine
    Article Snippet: Extracted RNAs were subjected to bisulfite conversion using the EZ RNA Methylation kit (Zymo). .. Library preparation for deep sequencing was done using the NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs).

    Article Title: The tRNA methyltransferase Dnmt2 is required for accurate polypeptide synthesis during haematopoiesis
    Article Snippet: RNA bisulfite sequencing Bisulfite conversion was performed using the EZ RNA Methylation™ Kit (Zymo Research). .. Amplicons for 454 (Roche) sequencing were generated and analyzed as described previously (Tuorto et al , ).

    Article Title: Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs
    Article Snippet: Small and long fractions were DNase-digested and bisulfite converted using the EZ RNA Methylation Kit (Zymo Research). .. After stepwise RNA end repair and further purification, cDNA synthesis and library preparation was carried out using the NEBNext Small RNA Library Prep Set, followed by paired-end sequencing on an Illumina HiSeq 2000 platform.

    Article Title: Division of labour: tRNA methylation by the NSun2 tRNA methyltransferases Trm4a and Trm4b in fission yeast
    Article Snippet: Extracted RNAs were subjected to bisulfite conversion using the EZ RNA Methylation kit (Zymo). .. Library preparation for deep sequencing was done using the NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs).

    Article Title: LXR/ApoE activation restricts innate immune suppression in cancer
    Article Snippet: Paragraph title: MDSC mRNA sequencing library preparation ... RNA was extracted from MDSCs using the Total RNA Extraction Kit (17200, Noragen), then purified and concentrated using the RNA Clean & Concentrator -5 Kit (R1016, Zymo Research).

    Article Title: Long non-coding RNAs as targets for cytosine methylation
    Article Snippet: .. Bisulfite sequencing To establish the BS sequencing protocol, total RNA from E. coli was subjected to either one round of incubation for 5 min at 70°C followed by 90 min at 64°C ( ) or three cycles of alternating incubations of 5 min at 70°C/45 min at 64°C ( ) using the EZ RNA Methylation Kit, Beta Version (Zymo Research) according to the manufacturer’s instructions. ..

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo. .. Amplicons for 454 (Roche) sequencing were generated by PCR as described in Tuorto et al ( ).

    Sonication:

    Article Title: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan
    Article Snippet: Alternatively, the EZ RNA Methylation Kit (Zymo Research) was used for bisulfite treatment and purification of converted RNA according to the instructions provided by the manufacturer. .. For C. elegans , ~200 whole young adult hermaphrodites were washed 3 × in PBS and lysed directly in Trizol by 30 cycles of sonication (30 s on, 30 s off) in a Bioruptor (Diagenode).

    Binding Assay:

    Article Title: Long non-coding RNAs as targets for cytosine methylation
    Article Snippet: Bisulfite sequencing To establish the BS sequencing protocol, total RNA from E. coli was subjected to either one round of incubation for 5 min at 70°C followed by 90 min at 64°C ( ) or three cycles of alternating incubations of 5 min at 70°C/45 min at 64°C ( ) using the EZ RNA Methylation Kit, Beta Version (Zymo Research) according to the manufacturer’s instructions. .. Removal of bisulfite, desulphonation and washing steps were performed by binding the RNA to spin columns provided in the kit.

    RNA Sequencing Assay:

    Article Title: LXR/ApoE activation restricts innate immune suppression in cancer
    Article Snippet: RNA was extracted from MDSCs using the Total RNA Extraction Kit (17200, Noragen), then purified and concentrated using the RNA Clean & Concentrator -5 Kit (R1016, Zymo Research). .. The RNA samples were depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (MRZG12324, Illumina) then reverse transcribed and prepared for sequencing using the ScriptSeq v2 RNA-Seq Library Preparation Kit ( , Epicenter).

    Methylation:

    Article Title: Comprehensive DNA methylation analysis of the Aedes aegypti genome
    Article Snippet: .. Bisulfite conversion was performed using the EZ RNA Methylation Kit (Zymo Research). .. Amplicons for 454 (Roche) sequencing were generated and analyzed as described previously .

    Article Title: Distinct 5-methylcytosine profiles in poly(A) RNA from mouse embryonic stem cells and brain
    Article Snippet: .. Bisulfite treatment Bisulfite treatment was performed as described previously [ ] using the EZ RNA methylation Kit (Zymo Research). ..

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: .. RNA bisulfite sequencing Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo. .. Amplicons for 454 (Roche) sequencing were generated by PCR as described in Tuorto et al ( ).

    Article Title: Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine
    Article Snippet: .. Extracted RNAs were subjected to bisulfite conversion using the EZ RNA Methylation kit (Zymo). .. 3′ dephosphorylation and 5′ phosphorylation were performed using T4 polynucleotide kinase (TaKaRa).

    Article Title: The tRNA methyltransferase Dnmt2 is required for accurate polypeptide synthesis during haematopoiesis
    Article Snippet: .. RNA bisulfite sequencing Bisulfite conversion was performed using the EZ RNA Methylation™ Kit (Zymo Research). .. Amplicons for 454 (Roche) sequencing were generated and analyzed as described previously (Tuorto et al , ).

    Article Title: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan
    Article Snippet: .. Alternatively, the EZ RNA Methylation Kit (Zymo Research) was used for bisulfite treatment and purification of converted RNA according to the instructions provided by the manufacturer. ..

    Article Title: DEPOSITION OF 5-METHYLCYTOSINE ON ENHANCER RNAs ENABLES THE COACTIVATOR FUNCTION OF PGC-1α
    Article Snippet: .. Bisulfite conversion of RNA was analyzed with the EZ RNA Methylation Kit (Zymo Research) according to the manufacturer’s recommendations. .. (49K, docx)

    Article Title: Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs
    Article Snippet: .. Small and long fractions were DNase-digested and bisulfite converted using the EZ RNA Methylation Kit (Zymo Research). .. After stepwise RNA end repair and further purification, cDNA synthesis and library preparation was carried out using the NEBNext Small RNA Library Prep Set, followed by paired-end sequencing on an Illumina HiSeq 2000 platform.

    Article Title: Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs
    Article Snippet: .. 454 Life Sciences (Roche) bisulfite sequencing was performed using the EZ RNA Methylation Kit (Zymo Research). .. PCR primers are provided in .

    Article Title: Division of labour: tRNA methylation by the NSun2 tRNA methyltransferases Trm4a and Trm4b in fission yeast
    Article Snippet: .. Extracted RNAs were subjected to bisulfite conversion using the EZ RNA Methylation kit (Zymo). .. 3′ dephosphorylation and 5′ phosphorylation were performed using T4 polynucleotide kinase (TaKaRa).

    Article Title: Long non-coding RNAs as targets for cytosine methylation
    Article Snippet: .. Bisulfite sequencing To establish the BS sequencing protocol, total RNA from E. coli was subjected to either one round of incubation for 5 min at 70°C followed by 90 min at 64°C ( ) or three cycles of alternating incubations of 5 min at 70°C/45 min at 64°C ( ) using the EZ RNA Methylation Kit, Beta Version (Zymo Research) according to the manufacturer’s instructions. ..

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: .. Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo. .. Amplicons for 454 (Roche) sequencing were generated by PCR as described in Tuorto et al ( ).

    Isolation:

    Article Title: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan
    Article Snippet: Total RNA or RNA isolated from polysome or monosome fractions was purified using Trizol (Life Technologies), DNAse I (Thermo Scientific) digested for 1 h, phenol–chloroform extracted, isopropanol precipitated and dissolved in 20 μl water. .. Alternatively, the EZ RNA Methylation Kit (Zymo Research) was used for bisulfite treatment and purification of converted RNA according to the instructions provided by the manufacturer.

    Article Title: Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs
    Article Snippet: Paragraph title: Cell culture, RNA isolation, and library preparation ... Small and long fractions were DNase-digested and bisulfite converted using the EZ RNA Methylation Kit (Zymo Research).

    Article Title: LXR/ApoE activation restricts innate immune suppression in cancer
    Article Snippet: MDSCs were isolated using the Myeloid-Derived Suppressor Cell Isolation Kit (130-094-538, Miltenyi Biotec). .. RNA was extracted from MDSCs using the Total RNA Extraction Kit (17200, Noragen), then purified and concentrated using the RNA Clean & Concentrator -5 Kit (R1016, Zymo Research).

    Mouse Assay:

    Article Title: LXR/ApoE activation restricts innate immune suppression in cancer
    Article Snippet: Peripheral blood collected from mice treated with either control or RGX 104 (100 mg/kg) for six days was depleted of erythrocytes through treatment with 1X ACK Lysing Buffer (10-548E, Lonza). .. RNA was extracted from MDSCs using the Total RNA Extraction Kit (17200, Noragen), then purified and concentrated using the RNA Clean & Concentrator -5 Kit (R1016, Zymo Research).

    Polymerase Chain Reaction:

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: RNA bisulfite sequencing Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo. .. Amplicons for 454 (Roche) sequencing were generated by PCR as described in Tuorto et al ( ).

    Article Title: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan
    Article Snippet: Five μl was subjected to a second round of PCR as above using barcoded primers and only 30 cycles in the second cycling step (**). .. Alternatively, the EZ RNA Methylation Kit (Zymo Research) was used for bisulfite treatment and purification of converted RNA according to the instructions provided by the manufacturer.

    Article Title: Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs
    Article Snippet: 454 Life Sciences (Roche) bisulfite sequencing was performed using the EZ RNA Methylation Kit (Zymo Research). .. PCR primers are provided in .

    Article Title: Queuosine‐modified tRNAs confer nutritional control of protein translation
    Article Snippet: Bisulfite conversion was performed using the EZ RNA Methylation™ Kit—Zymo. .. Amplicons for 454 (Roche) sequencing were generated by PCR as described in Tuorto et al ( ).

    De-Phosphorylation Assay:

    Article Title: Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine
    Article Snippet: Extracted RNAs were subjected to bisulfite conversion using the EZ RNA Methylation kit (Zymo). .. 3′ dephosphorylation and 5′ phosphorylation were performed using T4 polynucleotide kinase (TaKaRa).

    Article Title: Division of labour: tRNA methylation by the NSun2 tRNA methyltransferases Trm4a and Trm4b in fission yeast
    Article Snippet: Extracted RNAs were subjected to bisulfite conversion using the EZ RNA Methylation kit (Zymo). .. 3′ dephosphorylation and 5′ phosphorylation were performed using T4 polynucleotide kinase (TaKaRa).

    Purification:

    Article Title: Distinct 5-methylcytosine profiles in poly(A) RNA from mouse embryonic stem cells and brain
    Article Snippet: Bisulfite treatment Bisulfite treatment was performed as described previously [ ] using the EZ RNA methylation Kit (Zymo Research). .. RNA separation from bisulfite solution, desulfonation, and purification were performed using the kit.

    Article Title: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan
    Article Snippet: .. Alternatively, the EZ RNA Methylation Kit (Zymo Research) was used for bisulfite treatment and purification of converted RNA according to the instructions provided by the manufacturer. ..

    Article Title: Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs
    Article Snippet: Small and long fractions were DNase-digested and bisulfite converted using the EZ RNA Methylation Kit (Zymo Research). .. After stepwise RNA end repair and further purification, cDNA synthesis and library preparation was carried out using the NEBNext Small RNA Library Prep Set, followed by paired-end sequencing on an Illumina HiSeq 2000 platform.

    Article Title: LXR/ApoE activation restricts innate immune suppression in cancer
    Article Snippet: .. RNA was extracted from MDSCs using the Total RNA Extraction Kit (17200, Noragen), then purified and concentrated using the RNA Clean & Concentrator -5 Kit (R1016, Zymo Research). .. The RNA samples were depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (MRZG12324, Illumina) then reverse transcribed and prepared for sequencing using the ScriptSeq v2 RNA-Seq Library Preparation Kit ( , Epicenter).

    RNA Extraction:

    Article Title: LXR/ApoE activation restricts innate immune suppression in cancer
    Article Snippet: .. RNA was extracted from MDSCs using the Total RNA Extraction Kit (17200, Noragen), then purified and concentrated using the RNA Clean & Concentrator -5 Kit (R1016, Zymo Research). .. The RNA samples were depleted of rRNA using the Ribo-Zero Gold rRNA Removal Kit (MRZG12324, Illumina) then reverse transcribed and prepared for sequencing using the ScriptSeq v2 RNA-Seq Library Preparation Kit ( , Epicenter).

    Quantitation Assay:

    Article Title: DEPOSITION OF 5-METHYLCYTOSINE ON ENHANCER RNAs ENABLES THE COACTIVATOR FUNCTION OF PGC-1α
    Article Snippet: Paragraph title: Quantitation of 5′ methylcytosine (m5 C) in RNA ... Bisulfite conversion of RNA was analyzed with the EZ RNA Methylation Kit (Zymo Research) according to the manufacturer’s recommendations.

    Spectrophotometry:

    Article Title: Distinct 5-methylcytosine profiles in poly(A) RNA from mouse embryonic stem cells and brain
    Article Snippet: Bisulfite treatment Bisulfite treatment was performed as described previously [ ] using the EZ RNA methylation Kit (Zymo Research). .. RNA quantity was determined by absorbance measurement at 260 nm using a Nanodrop UV/Vis spectrophotometer (PeqLab).

    Gel Extraction:

    Article Title: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan
    Article Snippet: Five μl complementary DNA (cDNA) was subjected to the following touchdown-PCR programme in a 25 μl reaction with KAPA2G Robust Polymerase and Buffer A (Kapa Biosystems): PCR products were gel purified using PCR- and Gel Extraction Kit (Favorgen) and eluded in 30 μl. .. Alternatively, the EZ RNA Methylation Kit (Zymo Research) was used for bisulfite treatment and purification of converted RNA according to the instructions provided by the manufacturer.

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  • Bioz Stars
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  • 95
    Zymo Research ez rna methylationtm kit
    Ez Rna Methylationtm Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ez rna methylationtm kit/product/Zymo Research
    Average 95 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    ez rna methylationtm kit - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

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