rna clean concentrator kit  (Zymo Research)


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    Name:
    EZ RNA Methylation Kit
    Description:
    The EZ RNA Methylation Kit features rapid and reliable bisulfite conversion of cytosines in RNA for methylation analysis The kit streamlines the three step process for complete conversion of cytosine in into uracil RNA denaturation and bisulfite conversion processes are combined into a single step No buffer preparation is necessary The RNA Conversion Reagent is provided ready to use simply add the reagent to an RNA sample and incubate as indicated The innovative in column desulphonation technology eliminates messy precipitation steps ensuring researchers obtain consistent results The product has been designed to minimize template degradation loss of RNA during treatment and clean up and to provide complete conversion of cytosine for accurate methylation analysis Recovered RNA is ideal for RT PCR sequencing library preparation and Next Gen sequencing
    Catalog Number:
    r5001
    Price:
    None
    Applications:
    Bisulfite Conversion
    Size:
    50 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research rna clean concentrator kit
    EZ RNA Methylation Kit
    The EZ RNA Methylation Kit features rapid and reliable bisulfite conversion of cytosines in RNA for methylation analysis The kit streamlines the three step process for complete conversion of cytosine in into uracil RNA denaturation and bisulfite conversion processes are combined into a single step No buffer preparation is necessary The RNA Conversion Reagent is provided ready to use simply add the reagent to an RNA sample and incubate as indicated The innovative in column desulphonation technology eliminates messy precipitation steps ensuring researchers obtain consistent results The product has been designed to minimize template degradation loss of RNA during treatment and clean up and to provide complete conversion of cytosine for accurate methylation analysis Recovered RNA is ideal for RT PCR sequencing library preparation and Next Gen sequencing
    https://www.bioz.com/result/rna clean concentrator kit/product/Zymo Research
    Average 97 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    rna clean concentrator kit - by Bioz Stars, 2020-10
    97/100 stars

    Images

    1) Product Images from "Determinants of tRNA Recognition by the Radical SAM Enzyme RlmN"

    Article Title: Determinants of tRNA Recognition by the Radical SAM Enzyme RlmN

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0167298

    Interrogation of chimeric tRNA substrates. (A) A representative 2D structure of tRNA. (B) In silico predicted representation of chimeric tRNA used in this study. Elements derived from substrate tRNA Gln UUG are in blue, while those deriving from non-substrate tRNA Gly CCC are in red. (C) End-point methylation of chimeric tRNA and tRNA Gln UUG ACSL RNA. Bars represent the mean ± s.d. of at least two replicates.
    Figure Legend Snippet: Interrogation of chimeric tRNA substrates. (A) A representative 2D structure of tRNA. (B) In silico predicted representation of chimeric tRNA used in this study. Elements derived from substrate tRNA Gln UUG are in blue, while those deriving from non-substrate tRNA Gly CCC are in red. (C) End-point methylation of chimeric tRNA and tRNA Gln UUG ACSL RNA. Bars represent the mean ± s.d. of at least two replicates.

    Techniques Used: In Silico, Derivative Assay, Countercurrent Chromatography, Methylation

    RlmN mediated methylation of in vitro transcribed tRNAs. (A) End-point methylation of in vitro transcribed RNAs. Bars represent the mean of at least two replicates ± s.d. (B) Radio HPLC analysis of nucleosides obtained by methylation and total RNA digestion of select tRNA substrates. Radioactive signal co-elutes with an authentic m 2 A standard. (C) Evaluation of reaction requirements for methylation of in vitro transcribed tRNA Gln UUG . Bars represent the mean of two replicates ± s.d.
    Figure Legend Snippet: RlmN mediated methylation of in vitro transcribed tRNAs. (A) End-point methylation of in vitro transcribed RNAs. Bars represent the mean of at least two replicates ± s.d. (B) Radio HPLC analysis of nucleosides obtained by methylation and total RNA digestion of select tRNA substrates. Radioactive signal co-elutes with an authentic m 2 A standard. (C) Evaluation of reaction requirements for methylation of in vitro transcribed tRNA Gln UUG . Bars represent the mean of two replicates ± s.d.

    Techniques Used: Methylation, In Vitro, High Performance Liquid Chromatography

    2) Product Images from "Antibiotic resistance evolved via inactivation of a ribosomal RNA methylating enzyme"

    Article Title: Antibiotic resistance evolved via inactivation of a ribosomal RNA methylating enzyme

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkw699

    Reduction of in vivo methylation at A2503 upon expression of BsubB supports the dominant negative function of the evolved variants. MALDI-TOF mass spectrum of the C2480–C2520 fragment of E. coli 23S rRNA isolated from the WT E. coli BW25113 strain carrying an ( A ) empty plasmid, or expressing ( B ) evolved BsubB variant. RNA was digested by RNase T 1 and analyzed by MALDI-TOF. Comparison between the insets shows a significant reduction in the amount of methylated 2503-m 2 AΨG-2505 fragment at m/z 1013.16 when the BsubB variant is overexpressed. ( C ) A list of the expected RNase T 1 digestion fragments based on the rRNA sequence with presently known nucleotide modifications. Cm is methylated cytosine, m 2 A is 2-methyladenosine, and Ψ indicates pseudouridine.
    Figure Legend Snippet: Reduction of in vivo methylation at A2503 upon expression of BsubB supports the dominant negative function of the evolved variants. MALDI-TOF mass spectrum of the C2480–C2520 fragment of E. coli 23S rRNA isolated from the WT E. coli BW25113 strain carrying an ( A ) empty plasmid, or expressing ( B ) evolved BsubB variant. RNA was digested by RNase T 1 and analyzed by MALDI-TOF. Comparison between the insets shows a significant reduction in the amount of methylated 2503-m 2 AΨG-2505 fragment at m/z 1013.16 when the BsubB variant is overexpressed. ( C ) A list of the expected RNase T 1 digestion fragments based on the rRNA sequence with presently known nucleotide modifications. Cm is methylated cytosine, m 2 A is 2-methyladenosine, and Ψ indicates pseudouridine.

    Techniques Used: In Vivo, Methylation, Expressing, Dominant Negative Mutation, Isolation, Plasmid Preparation, Variant Assay, Sequencing

    Related Articles

    Methylation Sequencing:

    Article Title: Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs
    Article Snippet: .. 454 Life Sciences (Roche) bisulfite sequencing was performed using the EZ RNA Methylation Kit (Zymo Research). .. PCR primers are provided in .

    Generated:

    Article Title: A Novel Caenorhabditis Elegans Proteinopathy Model Shows Changes in mRNA Translational Frameshifting During Aging
    Article Snippet: .. 0.5 μg total RNA were bisulfite-converted using the EZ RNA Methylation™ Kit (Zymo Research) and subsequently cDNA was generated using Super Script IV (Life Technologies). .. PCR using One Taq ® DNA Polymerase was performed to generate a 101 bp PCR product surrounding C2381 with forward and reverse primers specific for the cDNA after bisulfite conversion (i.e., 5’-GGGAGTAATTATGATTTTTCTAAGGTAG-3’ and 5’-ATAATAAATAAAAACAATAAAAATCTCACTAATCCATTCATACAC-3’).

    Methylation:

    Article Title: Dynamic modulation of Dnmt2-dependent tRNA methylation by the micronutrient queuine
    Article Snippet: .. Extracted RNAs were subjected to bisulfite conversion using the EZ RNA Methylation kit (Zymo). .. 3′ dephosphorylation and 5′ phosphorylation were performed using T4 polynucleotide kinase (TaKaRa).

    Article Title: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan
    Article Snippet: .. Alternatively, the EZ RNA Methylation Kit (Zymo Research) was used for bisulfite treatment and purification of converted RNA according to the instructions provided by the manufacturer. ..

    Article Title: DEPOSITION OF 5-METHYLCYTOSINE ON ENHANCER RNAs ENABLES THE COACTIVATOR FUNCTION OF PGC-1α
    Article Snippet: .. Bisulfite conversion of RNA was analyzed with the EZ RNA Methylation Kit (Zymo Research) according to the manufacturer’s recommendations. .. (49K, docx)

    Article Title: Statistically robust methylation calling for whole-transcriptome bisulfite sequencing reveals distinct methylation patterns for mouse RNAs
    Article Snippet: .. 454 Life Sciences (Roche) bisulfite sequencing was performed using the EZ RNA Methylation Kit (Zymo Research). .. PCR primers are provided in .

    Article Title: Translational adaptation to heat stress is mediated by 5-methylcytosine RNA modification in Caenorhabditis elegans
    Article Snippet: .. DNase-treated samples were bisulfite-converted using an EZ RNA Methylation Kit (Zymo Research), following the manufacturer’s manual. .. As a final step before library preparation, a stepwise RNA end repair was carried out using T4 polynucleotide kinase (TaKaRa).

    Article Title: A Novel Caenorhabditis Elegans Proteinopathy Model Shows Changes in mRNA Translational Frameshifting During Aging
    Article Snippet: .. 0.5 μg total RNA were bisulfite-converted using the EZ RNA Methylation™ Kit (Zymo Research) and subsequently cDNA was generated using Super Script IV (Life Technologies). .. PCR using One Taq ® DNA Polymerase was performed to generate a 101 bp PCR product surrounding C2381 with forward and reverse primers specific for the cDNA after bisulfite conversion (i.e., 5’-GGGAGTAATTATGATTTTTCTAAGGTAG-3’ and 5’-ATAATAAATAAAAACAATAAAAATCTCACTAATCCATTCATACAC-3’).

    Article Title: Depletion of TRDMT1 affects 5-methylcytosine modification of mRNA and inhibits HEK293 cell proliferation and migration.
    Article Snippet: .. Human TRDMT1 is a transfer RNA (tRNA) methyltransferase for cytosine-5 methylation and has been suggested to be involved in the regulation of numerous developmental processes. .. Human TRDMT1 is a transfer RNA (tRNA) methyltransferase for cytosine-5 methylation and has been suggested to be involved in the regulation of numerous developmental processes.

    Pyromark Assay:

    Article Title: Depletion of TRDMT1 affects 5-methylcytosine modification of mRNA and inhibits HEK293 cell proliferation and migration.
    Article Snippet: .. Human TRDMT1 is a transfer RNA (tRNA) methyltransferase for cytosine-5 methylation and has been suggested to be involved in the regulation of numerous developmental processes. .. Human TRDMT1 is a transfer RNA (tRNA) methyltransferase for cytosine-5 methylation and has been suggested to be involved in the regulation of numerous developmental processes.

    Purification:

    Article Title: Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan
    Article Snippet: .. Alternatively, the EZ RNA Methylation Kit (Zymo Research) was used for bisulfite treatment and purification of converted RNA according to the instructions provided by the manufacturer. ..

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  • About
  • News
  • Press Release
  • Team
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  • Contact
  • Bioz Stars
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  • 97
    Zymo Research rna clean concentrator kit
    Interrogation of chimeric <t>tRNA</t> substrates. (A) A representative 2D structure of tRNA. (B) In silico predicted representation of chimeric tRNA used in this study. Elements derived from substrate tRNA Gln UUG are in blue, while those deriving from non-substrate tRNA Gly CCC are in red. (C) End-point methylation of chimeric tRNA and tRNA Gln UUG ACSL <t>RNA.</t> Bars represent the mean ± s.d. of at least two replicates.
    Rna Clean Concentrator Kit, supplied by Zymo Research, used in various techniques. Bioz Stars score: 97/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna clean concentrator kit/product/Zymo Research
    Average 97 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    rna clean concentrator kit - by Bioz Stars, 2020-10
    97/100 stars
      Buy from Supplier

    Image Search Results


    Interrogation of chimeric tRNA substrates. (A) A representative 2D structure of tRNA. (B) In silico predicted representation of chimeric tRNA used in this study. Elements derived from substrate tRNA Gln UUG are in blue, while those deriving from non-substrate tRNA Gly CCC are in red. (C) End-point methylation of chimeric tRNA and tRNA Gln UUG ACSL RNA. Bars represent the mean ± s.d. of at least two replicates.

    Journal: PLoS ONE

    Article Title: Determinants of tRNA Recognition by the Radical SAM Enzyme RlmN

    doi: 10.1371/journal.pone.0167298

    Figure Lengend Snippet: Interrogation of chimeric tRNA substrates. (A) A representative 2D structure of tRNA. (B) In silico predicted representation of chimeric tRNA used in this study. Elements derived from substrate tRNA Gln UUG are in blue, while those deriving from non-substrate tRNA Gly CCC are in red. (C) End-point methylation of chimeric tRNA and tRNA Gln UUG ACSL RNA. Bars represent the mean ± s.d. of at least two replicates.

    Article Snippet: The tRNA used in in vitro methylation assays was purified using Zymo Research RNA Clean & Concentrator kit unless otherwise noted.

    Techniques: In Silico, Derivative Assay, Countercurrent Chromatography, Methylation

    RlmN mediated methylation of in vitro transcribed tRNAs. (A) End-point methylation of in vitro transcribed RNAs. Bars represent the mean of at least two replicates ± s.d. (B) Radio HPLC analysis of nucleosides obtained by methylation and total RNA digestion of select tRNA substrates. Radioactive signal co-elutes with an authentic m 2 A standard. (C) Evaluation of reaction requirements for methylation of in vitro transcribed tRNA Gln UUG . Bars represent the mean of two replicates ± s.d.

    Journal: PLoS ONE

    Article Title: Determinants of tRNA Recognition by the Radical SAM Enzyme RlmN

    doi: 10.1371/journal.pone.0167298

    Figure Lengend Snippet: RlmN mediated methylation of in vitro transcribed tRNAs. (A) End-point methylation of in vitro transcribed RNAs. Bars represent the mean of at least two replicates ± s.d. (B) Radio HPLC analysis of nucleosides obtained by methylation and total RNA digestion of select tRNA substrates. Radioactive signal co-elutes with an authentic m 2 A standard. (C) Evaluation of reaction requirements for methylation of in vitro transcribed tRNA Gln UUG . Bars represent the mean of two replicates ± s.d.

    Article Snippet: The tRNA used in in vitro methylation assays was purified using Zymo Research RNA Clean & Concentrator kit unless otherwise noted.

    Techniques: Methylation, In Vitro, High Performance Liquid Chromatography

    Reduction of in vivo methylation at A2503 upon expression of BsubB supports the dominant negative function of the evolved variants. MALDI-TOF mass spectrum of the C2480–C2520 fragment of E. coli 23S rRNA isolated from the WT E. coli BW25113 strain carrying an ( A ) empty plasmid, or expressing ( B ) evolved BsubB variant. RNA was digested by RNase T 1 and analyzed by MALDI-TOF. Comparison between the insets shows a significant reduction in the amount of methylated 2503-m 2 AΨG-2505 fragment at m/z 1013.16 when the BsubB variant is overexpressed. ( C ) A list of the expected RNase T 1 digestion fragments based on the rRNA sequence with presently known nucleotide modifications. Cm is methylated cytosine, m 2 A is 2-methyladenosine, and Ψ indicates pseudouridine.

    Journal: Nucleic Acids Research

    Article Title: Antibiotic resistance evolved via inactivation of a ribosomal RNA methylating enzyme

    doi: 10.1093/nar/gkw699

    Figure Lengend Snippet: Reduction of in vivo methylation at A2503 upon expression of BsubB supports the dominant negative function of the evolved variants. MALDI-TOF mass spectrum of the C2480–C2520 fragment of E. coli 23S rRNA isolated from the WT E. coli BW25113 strain carrying an ( A ) empty plasmid, or expressing ( B ) evolved BsubB variant. RNA was digested by RNase T 1 and analyzed by MALDI-TOF. Comparison between the insets shows a significant reduction in the amount of methylated 2503-m 2 AΨG-2505 fragment at m/z 1013.16 when the BsubB variant is overexpressed. ( C ) A list of the expected RNase T 1 digestion fragments based on the rRNA sequence with presently known nucleotide modifications. Cm is methylated cytosine, m 2 A is 2-methyladenosine, and Ψ indicates pseudouridine.

    Article Snippet: HPLC separation and identification of methylated adenosines The methylated rRNA from WT Bsub_RlmN assay mixtures was purified using the RNA Clean & Concentrator kit (Zymo Research).

    Techniques: In Vivo, Methylation, Expressing, Dominant Negative Mutation, Isolation, Plasmid Preparation, Variant Assay, Sequencing