ez 96 dna magprep methylation kit  (Zymo Research)


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    Name:
    EZ 96 DNA Methylation MagPrep
    Description:
    The EZ 96 DNA Methylation MagPrep features a high throughput 96 well simplified procedure that streamlines bisulfite conversion of DNA The kit is based on the three step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil Desulphonation and clean up of the converted DNA is performed while bound to the MagBinding Beads The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean up while ensuring complete conversion of the DNA Purified converted DNA is ideal for downstream analyses including PCR amplification endonuclease digestion sequencing microarrays etc
    Catalog Number:
    d5040
    Price:
    None
    Applications:
    Bisulfite Conversion
    Size:
    4 x 96 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research ez 96 dna magprep methylation kit
    EZ 96 DNA Methylation MagPrep
    The EZ 96 DNA Methylation MagPrep features a high throughput 96 well simplified procedure that streamlines bisulfite conversion of DNA The kit is based on the three step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil Desulphonation and clean up of the converted DNA is performed while bound to the MagBinding Beads The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean up while ensuring complete conversion of the DNA Purified converted DNA is ideal for downstream analyses including PCR amplification endonuclease digestion sequencing microarrays etc
    https://www.bioz.com/result/ez 96 dna magprep methylation kit/product/Zymo Research
    Average 95 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    ez 96 dna magprep methylation kit - by Bioz Stars, 2020-10
    95/100 stars

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    Related Articles

    DNA Methylation Assay:

    Article Title: Extreme phenotypes define epigenetic and metabolic signatures in cardiometabolic syndrome
    Article Snippet: .. Samples were bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) following the manufacturer’s instructions with optimized incubation conditions (i.e., 16 cycles of 95C for 30 s, 50C for 60 min; followed by 4C until further processing). .. Purified bisulfite-treated DNA was eluted in 15 mL of M-Elution Buffer (Zymo Research).

    Article Title: The genomic and epigenomic evolutionary history of papillary renal cell carcinomas
    Article Snippet: .. Methylation analysis 400 ng of sample DNA, according to Quant-iTPicoGreen dsDNA quantitation (Life Technologies, Grand Island, NY), was treated with sodium bisulfite using the EZ-96 DNA Methylation MagPrep Kit (Zymo Research, Irvine, CA) according to manufacturer-provided protocol. .. Bisulfite conversion modifies non-methylated cytosines into uracil, leaving 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) unchanged.

    Article Title: Highly scalable generation of DNA methylation profiles in single cells
    Article Snippet: .. A second 96-well plate was prepared with each well containing digestion reagents as described by the manufacturer’s protocol for the EZ-96 DNA Methylation MagPrep Kit (Zymo, Cat. D5040) at one-fifth the volumes (for a total of 5 uL per well). ..

    Article Title: Human exposure to trichloroethylene is associated with increased variability of blood DNA methylation that is enriched in genes and pathways related to autoimmune disease and cancer
    Article Snippet: .. 1000ng of sample DNA, according to Quant-iT PicoGreen dsDNA quantitation (Life Technologies, Grand Island, NY), was treated with sodium bisulfite using the EZ-96 DNA Methylation MagPrep Kit (Zymo Research, Irvine, CA) according to manufacturer-provided protocol. .. Bisulfite converted DNA samples were hybridized to the 12 sample Illumina HumanMethylation450 BeadChips using the Infinium HD Methylation protocol (Document 15,019,519 v01).

    Article Title: Highly scalable generation of DNA methylation profiles in single cells
    Article Snippet: .. Nuclei were then processed following manufacturer’s protocol for the EZ-96 DNA Methylation MagPrep Kit, with volumes reduced to one-fifth those described by the manufacturer to allow for single-well reaction processing, and other slight modifications. .. Following the final post-bisulfite library cleanup, each well was eluted in 25 uL of Zymo M-Elution Buffer and transferred to a well in a 96-well plate prepared with the following reaction mixture for linear amplification: 16 uL PCR-clean ddH2O, 5 uL 10X NEBuffer 2.1 (NEB, Cat. B7202), 2 uL 10 mM dNTP mix (NEB, Cat. N0447), and 2 uL of 10 uM random nonamer primer with a partial sequence of the Illumina Standard Read 2 sequencing primer (9NP, 3′-NNNNNNNNNAGATCGGAAGAGCACACGTCTG-5′).

    Incubation:

    Article Title: Extreme phenotypes define epigenetic and metabolic signatures in cardiometabolic syndrome
    Article Snippet: .. Samples were bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) following the manufacturer’s instructions with optimized incubation conditions (i.e., 16 cycles of 95C for 30 s, 50C for 60 min; followed by 4C until further processing). .. Purified bisulfite-treated DNA was eluted in 15 mL of M-Elution Buffer (Zymo Research).

    other:

    Article Title: Extreme phenotypes define epigenetic and metabolic signatures in cardiometabolic syndrome
    Article Snippet: 500ng of DNA for each monocyte and neutrophil sample was randomly dispensed onto a 96-well plate to reduce batch effects.

    Quantitation Assay:

    Article Title: The genomic and epigenomic evolutionary history of papillary renal cell carcinomas
    Article Snippet: .. Methylation analysis 400 ng of sample DNA, according to Quant-iTPicoGreen dsDNA quantitation (Life Technologies, Grand Island, NY), was treated with sodium bisulfite using the EZ-96 DNA Methylation MagPrep Kit (Zymo Research, Irvine, CA) according to manufacturer-provided protocol. .. Bisulfite conversion modifies non-methylated cytosines into uracil, leaving 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) unchanged.

    Article Title: Human exposure to trichloroethylene is associated with increased variability of blood DNA methylation that is enriched in genes and pathways related to autoimmune disease and cancer
    Article Snippet: .. 1000ng of sample DNA, according to Quant-iT PicoGreen dsDNA quantitation (Life Technologies, Grand Island, NY), was treated with sodium bisulfite using the EZ-96 DNA Methylation MagPrep Kit (Zymo Research, Irvine, CA) according to manufacturer-provided protocol. .. Bisulfite converted DNA samples were hybridized to the 12 sample Illumina HumanMethylation450 BeadChips using the Infinium HD Methylation protocol (Document 15,019,519 v01).

    Methylation:

    Article Title: Implementing a method for studying longitudinal DNA methylation variability in association with age
    Article Snippet: .. The DNA collected from blood samples were bisulfite converted using EZ-96 DNA MagPrep methylation kit (Zymo Research Corp., Orange, CA) and methylation profiles were measured by Illumina HumanMethylation450 BeadChip (Illumina Inc., San Diego, CA). .. The preprocessing of methylation data was performed using the R package RnBeads [ ].

    Article Title: The genomic and epigenomic evolutionary history of papillary renal cell carcinomas
    Article Snippet: .. Methylation analysis 400 ng of sample DNA, according to Quant-iTPicoGreen dsDNA quantitation (Life Technologies, Grand Island, NY), was treated with sodium bisulfite using the EZ-96 DNA Methylation MagPrep Kit (Zymo Research, Irvine, CA) according to manufacturer-provided protocol. .. Bisulfite conversion modifies non-methylated cytosines into uracil, leaving 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) unchanged.

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