ez 96 dna magprep methylation kit  (Zymo Research)


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    Name:
    EZ 96 DNA Methylation MagPrep
    Description:
    The EZ 96 DNA Methylation MagPrep features a high throughput 96 well simplified procedure that streamlines bisulfite conversion of DNA The kit is based on the three step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil Desulphonation and clean up of the converted DNA is performed while bound to the MagBinding Beads The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean up while ensuring complete conversion of the DNA Purified converted DNA is ideal for downstream analyses including PCR amplification endonuclease digestion sequencing microarrays etc
    Catalog Number:
    d5040
    Price:
    None
    Applications:
    Bisulfite Conversion
    Size:
    4 x 96 units
    Category:
    Life Science Reagents and Media
    Buy from Supplier


    Structured Review

    Zymo Research ez 96 dna magprep methylation kit
    EZ 96 DNA Methylation MagPrep
    The EZ 96 DNA Methylation MagPrep features a high throughput 96 well simplified procedure that streamlines bisulfite conversion of DNA The kit is based on the three step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil Desulphonation and clean up of the converted DNA is performed while bound to the MagBinding Beads The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean up while ensuring complete conversion of the DNA Purified converted DNA is ideal for downstream analyses including PCR amplification endonuclease digestion sequencing microarrays etc
    https://www.bioz.com/result/ez 96 dna magprep methylation kit/product/Zymo Research
    Average 95 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    ez 96 dna magprep methylation kit - by Bioz Stars, 2020-02
    95/100 stars

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    Related Articles

    Flow Cytometry:

    Article Title: Highly scalable generation of DNA methylation profiles in single cells
    Article Snippet: Fluorescence-assisted nuclei sorting (FANS) was performed with a Sony SH800 flow sorter to sort 2,500 single nuclei into each well in fast sort mode ( ). .. A second 96-well plate was prepared with each well containing digestion reagents as described by the manufacturer’s protocol for the EZ-96 DNA Methylation MagPrep Kit (Zymo, Cat. D5040) at one-fifth the volumes (for a total of 5 uL per well).

    Amplification:

    Article Title: Increased DNA methylation variability in type 1 diabetes across three immune effector cell types
    Article Snippet: Illumina Infinium HumanMethylation450 assay Genomic DNA was bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) according to the manufacturer's instructions. .. In brief, 4 μl of bisulfite-converted DNA was isothermally amplified, enzymatically fragmented and precipitated.

    Article Title: Highly scalable generation of DNA methylation profiles in single cells
    Article Snippet: This cytosine-depleted approach improved downstream PCR amplification and decreased library generation costs compared to previous methylated adaptor attempts . .. A second 96-well plate was prepared with each well containing digestion reagents as described by the manufacturer’s protocol for the EZ-96 DNA Methylation MagPrep Kit (Zymo, Cat. D5040) at one-fifth the volumes (for a total of 5 uL per well).

    Article Title: Female chromosome X mosaicism is age-related and preferentially affects the inactivated X chromosome
    Article Snippet: Methylation arrays After Quant-iT PicoGreen dsDNA quantitation (Life Technologies, Grand Island, NY), 1,000 ng of sample DNA were treated with sodium bisulfite using the EZ-96 DNA Methylation MagPrep Kit (Zymo Research, Irvine, CA) to convert unmethylated cytosine residues to uracils (detected as thymidines), leaving 5′-methylcytosines residues unaffected. .. Bisulfite-treated samples were denatured, neutralized and then whole-genome amplified, isothermally, to increase the amount of DNA template.

    Article Title: A donor-specific epigenetic classifier for acute graft-versus-host disease severity in hematopoietic stem cell transplantation
    Article Snippet: Illumina Infinium HumanMethylation450 assay Genomic DNA was bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) according to the manufacturer’s instructions. .. In brief, 4 μL of bisulfite-converted DNA was isothermally amplified, enzymatically fragmented, and precipitated.

    Fluorescence:

    Article Title: Highly scalable generation of DNA methylation profiles in single cells
    Article Snippet: Fluorescence-assisted nuclei sorting (FANS) was performed with a Sony SH800 flow sorter to sort 2,500 single nuclei into each well in fast sort mode ( ). .. A second 96-well plate was prepared with each well containing digestion reagents as described by the manufacturer’s protocol for the EZ-96 DNA Methylation MagPrep Kit (Zymo, Cat. D5040) at one-fifth the volumes (for a total of 5 uL per well).

    Methylation:

    Article Title: Highly scalable generation of DNA methylation profiles in single cells
    Article Snippet: This cytosine-depleted approach improved downstream PCR amplification and decreased library generation costs compared to previous methylated adaptor attempts . .. A second 96-well plate was prepared with each well containing digestion reagents as described by the manufacturer’s protocol for the EZ-96 DNA Methylation MagPrep Kit (Zymo, Cat. D5040) at one-fifth the volumes (for a total of 5 uL per well).

    Article Title: Female chromosome X mosaicism is age-related and preferentially affects the inactivated X chromosome
    Article Snippet: .. Methylation arrays After Quant-iT PicoGreen dsDNA quantitation (Life Technologies, Grand Island, NY), 1,000 ng of sample DNA were treated with sodium bisulfite using the EZ-96 DNA Methylation MagPrep Kit (Zymo Research, Irvine, CA) to convert unmethylated cytosine residues to uracils (detected as thymidines), leaving 5′-methylcytosines residues unaffected. .. Bisulfite-treated samples were denatured, neutralized and then whole-genome amplified, isothermally, to increase the amount of DNA template.

    Article Title: Epigenetic influences on aging: a longitudinal genome-wide methylation study in old Swedish twins
    Article Snippet: .. For each sample, 200 ng of DNA were bisulfite converted using the EZ-96 DNA MagPrep methylation kit (Zymo Research Corp., Orange, CA, USA) according to the manufacturer’s protocol optimized for Illumina’s Infinium 450K assay. .. The bisulfite converted DNA samples were hybridized to the Infinium HumanMethylation450 BeadChips by the University College London Genomics Core Facility according to Illumina’s Infinium HD protocol (Illumina Inc., San Diego, CA, USA).

    Article Title: Implementing a method for studying longitudinal DNA methylation variability in association with age
    Article Snippet: .. The DNA collected from blood samples were bisulfite converted using EZ-96 DNA MagPrep methylation kit (Zymo Research Corp., Orange, CA) and methylation profiles were measured by Illumina HumanMethylation450 BeadChip (Illumina Inc., San Diego, CA). .. The preprocessing of methylation data was performed using the R package RnBeads [ ].

    Article Title: Smoking-associated AHRR demethylation in cord blood DNA: impact of CD235a+ nucleated red blood cells
    Article Snippet: .. Methylation analysis Bisulfited-converted whole cord blood DNA (n = 43) treated with the EZ-96 DNA Methylation MagPrep kit (Zymo Research) was analyzed on the Illumina MethylationEPIC arrays (also known as 850K arrays) following manufacturer’s protocols. ..

    Incubation:

    Article Title: Genetic Drivers of Epigenetic and Transcriptional Variation in Human Immune Cells
    Article Snippet: .. Samples were bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) following the manufacturer’s instructions with optimized incubation conditions (i.e., 16 cycles of 95°C for 30 s, 50°C for 60 min; followed by 4°C until further processing). .. Purified bisulfite-treated DNA was eluted in 15 μL of M-Elution Buffer (Zymo Research).

    Article Title: Robust single-cell DNA methylome profiling with snmC-seq2
    Article Snippet: One hundred microliters of M-Wash Buffer (Zymo D5040-4) was added and the plates were centrifuged. .. Fifty microliters of M-Desulphonation Buffer (Zymo D5040-5) was added and the plates were incubated at room temperature for 15 min, then centrifuged.

    Article Title: Robust single-cell DNA methylome profiling with snmC-seq2
    Article Snippet: .. Fifty microliters of M-Desulphonation Buffer (Zymo D5040-5) was added and the plates were incubated at room temperature for 15 min, then centrifuged. .. The plates were washed twice with 100 µL M-Wash Buffer, then eluted into a clean 384-well plate with 7 µL EB Buffer (Qiagen 19086) containing 500 nM RP-N or RP-H primers (primer sequences provided in ).

    Purification:

    Article Title: Increased DNA methylation variability in type 1 diabetes across three immune effector cell types
    Article Snippet: Illumina Infinium HumanMethylation450 assay Genomic DNA was bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) according to the manufacturer's instructions. .. We applied 500 ng of genomic DNA to bisulfite treatment, and eluted purified, bisulfite-converted DNA in 20 μl of M-Elution Buffer (Zymo Research).

    Article Title: Genetic Drivers of Epigenetic and Transcriptional Variation in Human Immune Cells
    Article Snippet: The DNA was purified through four successive steps with wash buffers in the DNeasy Mini spin columns, and finally eluted using a DNeasy membrane. .. Samples were bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) following the manufacturer’s instructions with optimized incubation conditions (i.e., 16 cycles of 95°C for 30 s, 50°C for 60 min; followed by 4°C until further processing).

    Article Title: A donor-specific epigenetic classifier for acute graft-versus-host disease severity in hematopoietic stem cell transplantation
    Article Snippet: Illumina Infinium HumanMethylation450 assay Genomic DNA was bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) according to the manufacturer’s instructions. .. We applied 500 ng or 250 ng of genomic DNA to bisulfite treatment, and eluted purified, bisulfite-converted DNA in 20 μL or 11 μL of M-Elution Buffer (Zymo Research), respectively.

    Modification:

    Article Title: Genetic Drivers of Epigenetic and Transcriptional Variation in Human Immune Cells
    Article Snippet: McGill–DNA was extracted from cell pellets of purified naive CD4+ T cells at McGill University using a protocol modified from the DNeasy Blood and Tissue Handbook (QIAGEN). .. Samples were bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) following the manufacturer’s instructions with optimized incubation conditions (i.e., 16 cycles of 95°C for 30 s, 50°C for 60 min; followed by 4°C until further processing).

    Quantitation Assay:

    Article Title: Female chromosome X mosaicism is age-related and preferentially affects the inactivated X chromosome
    Article Snippet: .. Methylation arrays After Quant-iT PicoGreen dsDNA quantitation (Life Technologies, Grand Island, NY), 1,000 ng of sample DNA were treated with sodium bisulfite using the EZ-96 DNA Methylation MagPrep Kit (Zymo Research, Irvine, CA) to convert unmethylated cytosine residues to uracils (detected as thymidines), leaving 5′-methylcytosines residues unaffected. .. Bisulfite-treated samples were denatured, neutralized and then whole-genome amplified, isothermally, to increase the amount of DNA template.

    Lysis:

    Article Title: Genetic Drivers of Epigenetic and Transcriptional Variation in Human Immune Cells
    Article Snippet: Briefly, cell pellets were lysed using proteinase K, RNase A, and Buffer AL lysis buffer, followed by precipitation in ethanol. .. Samples were bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) following the manufacturer’s instructions with optimized incubation conditions (i.e., 16 cycles of 95°C for 30 s, 50°C for 60 min; followed by 4°C until further processing).

    Polymerase Chain Reaction:

    Article Title: Highly scalable generation of DNA methylation profiles in single cells
    Article Snippet: This cytosine-depleted approach improved downstream PCR amplification and decreased library generation costs compared to previous methylated adaptor attempts . .. A second 96-well plate was prepared with each well containing digestion reagents as described by the manufacturer’s protocol for the EZ-96 DNA Methylation MagPrep Kit (Zymo, Cat. D5040) at one-fifth the volumes (for a total of 5 uL per well).

    Staining:

    Article Title: Highly scalable generation of DNA methylation profiles in single cells
    Article Snippet: All wells were then pooled and stained with DAPI as described for the first FANS sort. .. A second 96-well plate was prepared with each well containing digestion reagents as described by the manufacturer’s protocol for the EZ-96 DNA Methylation MagPrep Kit (Zymo, Cat. D5040) at one-fifth the volumes (for a total of 5 uL per well).

    DNA Methylation Assay:

    Article Title: Increased DNA methylation variability in type 1 diabetes across three immune effector cell types
    Article Snippet: .. Illumina Infinium HumanMethylation450 assay Genomic DNA was bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) according to the manufacturer's instructions. .. We applied 500 ng of genomic DNA to bisulfite treatment, and eluted purified, bisulfite-converted DNA in 20 μl of M-Elution Buffer (Zymo Research).

    Article Title: Genetic Drivers of Epigenetic and Transcriptional Variation in Human Immune Cells
    Article Snippet: .. Samples were bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) following the manufacturer’s instructions with optimized incubation conditions (i.e., 16 cycles of 95°C for 30 s, 50°C for 60 min; followed by 4°C until further processing). .. Purified bisulfite-treated DNA was eluted in 15 μL of M-Elution Buffer (Zymo Research).

    Article Title: Highly scalable generation of DNA methylation profiles in single cells
    Article Snippet: .. A second 96-well plate was prepared with each well containing digestion reagents as described by the manufacturer’s protocol for the EZ-96 DNA Methylation MagPrep Kit (Zymo, Cat. D5040) at one-fifth the volumes (for a total of 5 uL per well). ..

    Article Title: Female chromosome X mosaicism is age-related and preferentially affects the inactivated X chromosome
    Article Snippet: .. Methylation arrays After Quant-iT PicoGreen dsDNA quantitation (Life Technologies, Grand Island, NY), 1,000 ng of sample DNA were treated with sodium bisulfite using the EZ-96 DNA Methylation MagPrep Kit (Zymo Research, Irvine, CA) to convert unmethylated cytosine residues to uracils (detected as thymidines), leaving 5′-methylcytosines residues unaffected. .. Bisulfite-treated samples were denatured, neutralized and then whole-genome amplified, isothermally, to increase the amount of DNA template.

    Article Title: A donor-specific epigenetic classifier for acute graft-versus-host disease severity in hematopoietic stem cell transplantation
    Article Snippet: .. Illumina Infinium HumanMethylation450 assay Genomic DNA was bisulfite-converted using an EZ-96 DNA Methylation MagPrep Kit (Zymo Research) according to the manufacturer’s instructions. .. We applied 500 ng or 250 ng of genomic DNA to bisulfite treatment, and eluted purified, bisulfite-converted DNA in 20 μL or 11 μL of M-Elution Buffer (Zymo Research), respectively.

    Article Title: Epigenetic influences on aging: a longitudinal genome-wide methylation study in old Swedish twins
    Article Snippet: For each sample, 200 ng of DNA were bisulfite converted using the EZ-96 DNA MagPrep methylation kit (Zymo Research Corp., Orange, CA, USA) according to the manufacturer’s protocol optimized for Illumina’s Infinium 450K assay. .. DNA methylation levels of 485,512 CpGs were measured for each sample.

    Article Title: Implementing a method for studying longitudinal DNA methylation variability in association with age
    Article Snippet: DNA methylation data were obtained from the Swedish Adoption/Twin Study of Aging (SATSA) as described previously [ ]. .. The DNA collected from blood samples were bisulfite converted using EZ-96 DNA MagPrep methylation kit (Zymo Research Corp., Orange, CA) and methylation profiles were measured by Illumina HumanMethylation450 BeadChip (Illumina Inc., San Diego, CA).

    Article Title: Smoking-associated AHRR demethylation in cord blood DNA: impact of CD235a+ nucleated red blood cells
    Article Snippet: .. Methylation analysis Bisulfited-converted whole cord blood DNA (n = 43) treated with the EZ-96 DNA Methylation MagPrep kit (Zymo Research) was analyzed on the Illumina MethylationEPIC arrays (also known as 850K arrays) following manufacturer’s protocols. ..

    Article Title: Highly scalable generation of DNA methylation profiles in single cells
    Article Snippet: .. Nuclei were then processed following manufacturer’s protocol for the EZ-96 DNA Methylation MagPrep Kit, with volumes reduced to one-fifth those described by the manufacturer to allow for single-well reaction processing, and other slight modifications. .. Following the final post-bisulfite library cleanup, each well was eluted in 25 uL of Zymo M-Elution Buffer and transferred to a well in a 96-well plate prepared with the following reaction mixture for linear amplification: 16 uL PCR-clean ddH2O, 5 uL 10X NEBuffer 2.1 (NEB, Cat. B7202), 2 uL 10 mM dNTP mix (NEB, Cat. N0447), and 2 uL of 10 uM random nonamer primer with a partial sequence of the Illumina Standard Read 2 sequencing primer (9NP, 3′-NNNNNNNNNAGATCGGAAGAGCACACGTCTG-5′).

    Hybridization:

    Article Title: Female chromosome X mosaicism is age-related and preferentially affects the inactivated X chromosome
    Article Snippet: Methylation arrays After Quant-iT PicoGreen dsDNA quantitation (Life Technologies, Grand Island, NY), 1,000 ng of sample DNA were treated with sodium bisulfite using the EZ-96 DNA Methylation MagPrep Kit (Zymo Research, Irvine, CA) to convert unmethylated cytosine residues to uracils (detected as thymidines), leaving 5′-methylcytosines residues unaffected. .. The amplified product was enzymatically fragmented, precipitated and resuspended in hybridization buffer.

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  • 95
    Zymo Research ez 96 dna magprep methylation kit
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    https://www.bioz.com/result/ez 96 dna magprep methylation kit/product/Zymo Research
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