tgf β1 elisa kit  (Boster Bio)


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    Name:
    Human LAP TGF Beta1 ELISA Kit PicoKine
    Description:

    Catalog Number:
    EK1218
    Price:
    369.0
    Category:
    ELISA Kits
    Reactivity:
    Human
    Applications:
    ELISA
    Immunogen:
    Expression system for standard: sf21; Immunogen sequence: L30-R278
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    Structured Review

    Boster Bio tgf β1 elisa kit
    Human LAP TGF Beta1 ELISA Kit PicoKine

    https://www.bioz.com/result/tgf β1 elisa kit/product/Boster Bio
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tgf β1 elisa kit - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation"

    Article Title: Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation

    Journal: World Journal of Gastroenterology : WJG

    doi: 10.3748/wjg.15.4753

    Emodin decreases the levels of serum transforming growth factor-β1 (TGF-β1) and mRNA levels in liver tissues in the rat model. Serum and liver tissue were obtained from each treated rat. A: Levels of serum TGF-β1 were determined
    Figure Legend Snippet: Emodin decreases the levels of serum transforming growth factor-β1 (TGF-β1) and mRNA levels in liver tissues in the rat model. Serum and liver tissue were obtained from each treated rat. A: Levels of serum TGF-β1 were determined

    Techniques Used:

    2) Product Images from "The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats"

    Article Title: The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2014.1745

    (A) The effect of epigallocatechin-3-gallate (EGCG) on the serum levels of transforming growth factor β1 (TGF-β1), (B) interleukin (IL)-6, (C) IL-10, and (D) tumor necrosis factor α (TNF-α) at 15, 30, 60 and 120 days post-irradiation. (A) Serum TGF-β1 levels were significantly lower (p
    Figure Legend Snippet: (A) The effect of epigallocatechin-3-gallate (EGCG) on the serum levels of transforming growth factor β1 (TGF-β1), (B) interleukin (IL)-6, (C) IL-10, and (D) tumor necrosis factor α (TNF-α) at 15, 30, 60 and 120 days post-irradiation. (A) Serum TGF-β1 levels were significantly lower (p

    Techniques Used: Irradiation

    3) Product Images from "Targeting CXCR4 reverts the suppressive activity of T-regulatory cells in renal cancer"

    Article Title: Targeting CXCR4 reverts the suppressive activity of T-regulatory cells in renal cancer

    Journal: Oncotarget

    doi: 10.18632/oncotarget.20363

    TT-Tregs are more suppressive than PB and PT-Tregs in RCC patients (A) Demethylation rate of FOXP3 -TSDR in TT-Tregs and PB-Tregs versus HD-Tregs (2 TT-Tregs vs 4 PB-Tregs vs 3 HD respectively). (B) IFN-γ-Treg dependent ELISA and (C) TGF-β1-Treg dependent ELISA from 8 RCC patients.
    Figure Legend Snippet: TT-Tregs are more suppressive than PB and PT-Tregs in RCC patients (A) Demethylation rate of FOXP3 -TSDR in TT-Tregs and PB-Tregs versus HD-Tregs (2 TT-Tregs vs 4 PB-Tregs vs 3 HD respectively). (B) IFN-γ-Treg dependent ELISA and (C) TGF-β1-Treg dependent ELISA from 8 RCC patients.

    Techniques Used: Enzyme-linked Immunosorbent Assay

    4) Product Images from "The role of Nrf2/PIWIL2/purine metabolism axis in controlling radiation-induced lung fibrosis"

    Article Title: The role of Nrf2/PIWIL2/purine metabolism axis in controlling radiation-induced lung fibrosis

    Journal: American Journal of Cancer Research

    doi:

    The role of different PIWIL2 expression levels on the production of transforming growth factor-β1 (TGF-β1) in WI-38 cells after irradiation. PIWIL2 overexpression (PIWIL2-OE) and knockdown (PIWIL2-KD) were confirmed at the mRNA (A) and protein (B) levels by qRT-PCR and western blotting in WI-38 cells. The wild-type (WT) WI-38 cells were irradiated (IR) (6 Gy) in the presence or absence of 50 nM CDDO-Me. The PIWIL2-KD WI-38 cells were treated with 50 nM CDDO-Me following 6 Gy of IR. PIWIL2-OE WI-38 cells were only irradiated by 6 Gy X-rays. The expression levels of transforming growth factor-β1 (TGF-β1) in the culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA) (C). *P
    Figure Legend Snippet: The role of different PIWIL2 expression levels on the production of transforming growth factor-β1 (TGF-β1) in WI-38 cells after irradiation. PIWIL2 overexpression (PIWIL2-OE) and knockdown (PIWIL2-KD) were confirmed at the mRNA (A) and protein (B) levels by qRT-PCR and western blotting in WI-38 cells. The wild-type (WT) WI-38 cells were irradiated (IR) (6 Gy) in the presence or absence of 50 nM CDDO-Me. The PIWIL2-KD WI-38 cells were treated with 50 nM CDDO-Me following 6 Gy of IR. PIWIL2-OE WI-38 cells were only irradiated by 6 Gy X-rays. The expression levels of transforming growth factor-β1 (TGF-β1) in the culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA) (C). *P

    Techniques Used: Expressing, Irradiation, Over Expression, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

    The effects of PIWIL2 overexpression (PIWIL2-OE) and knockout (PIWIL2-KD) on the expression of purine metabolism rate-limiting enzymes were analyzed by qRT-PCR (A). The wild-type (WT) WI-38 cells were irradiated (6 Gy) in the presence or absence of 50 nM CDDO-Me. The PIWIL2-KD WI-38 cells were treated with 50 nM CDDO-Me following 6 Gy of irradiation (IR). The PIWIL2-OE WI-38 cells were irradiated (6 Gy) in the presence or absence of 5 mM mycophenolate mofetil (MMF). The WT WI-38 cells treated with CDDO-Me and MMF following 6 Gy X-ray exposure. The expression levels of transforming growth factor-β1 (TGF-β1) in the culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA) (B). *P
    Figure Legend Snippet: The effects of PIWIL2 overexpression (PIWIL2-OE) and knockout (PIWIL2-KD) on the expression of purine metabolism rate-limiting enzymes were analyzed by qRT-PCR (A). The wild-type (WT) WI-38 cells were irradiated (6 Gy) in the presence or absence of 50 nM CDDO-Me. The PIWIL2-KD WI-38 cells were treated with 50 nM CDDO-Me following 6 Gy of irradiation (IR). The PIWIL2-OE WI-38 cells were irradiated (6 Gy) in the presence or absence of 5 mM mycophenolate mofetil (MMF). The WT WI-38 cells treated with CDDO-Me and MMF following 6 Gy X-ray exposure. The expression levels of transforming growth factor-β1 (TGF-β1) in the culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA) (B). *P

    Techniques Used: Over Expression, Knock-Out, Expressing, Quantitative RT-PCR, Irradiation, Enzyme-linked Immunosorbent Assay

    Effects of different levels of Nrf2 on the production of transforming growth factor-β1 (TGF-β1) in WI-38 (A) and THP-1 (B) cells after irradiation. The wild-type (WT) cells were irradiated (IR) (6 Gy) in the presence or absence of 50 nM CDDO-Me. The irradiated Nrf2-KD cells were exposed to the same dose of X-ray as WT cells. The expression levels of TGF-β1 in the culture supernatant of WI-38 (A) and THP-1 (B) cells were detected by enzyme-linked immunosorbent assay (ELISA). *P
    Figure Legend Snippet: Effects of different levels of Nrf2 on the production of transforming growth factor-β1 (TGF-β1) in WI-38 (A) and THP-1 (B) cells after irradiation. The wild-type (WT) cells were irradiated (IR) (6 Gy) in the presence or absence of 50 nM CDDO-Me. The irradiated Nrf2-KD cells were exposed to the same dose of X-ray as WT cells. The expression levels of TGF-β1 in the culture supernatant of WI-38 (A) and THP-1 (B) cells were detected by enzyme-linked immunosorbent assay (ELISA). *P

    Techniques Used: Irradiation, Expressing, Enzyme-linked Immunosorbent Assay

    5) Product Images from "MiR-663 inhibits radiation-induced bystander effects by targeting TGFB1 in a feedback mode"

    Article Title: MiR-663 inhibits radiation-induced bystander effects by targeting TGFB1 in a feedback mode

    Journal: RNA Biology

    doi: 10.4161/rna.34345

    Proposed model for the function of miR-663 in TGF-β1-mediated RIBE. In irradiated cells, miR-663 downregulation leads to derepression of the TGFB1 transcript, partially resulting in increased TGF-β1 protein levels in the culture medium.
    Figure Legend Snippet: Proposed model for the function of miR-663 in TGF-β1-mediated RIBE. In irradiated cells, miR-663 downregulation leads to derepression of the TGFB1 transcript, partially resulting in increased TGF-β1 protein levels in the culture medium.

    Techniques Used: Irradiation

    TGF-β1 is a mediator of radiation-induced bystander effects. TGFB1 mRNA levels were detected using qRT-PCR in HeLa cells 2 h after exposure to different doses of X-ray irradiation. ( B ) TGFB1 mRNA levels were detected in HeLa cells exposed
    Figure Legend Snippet: TGF-β1 is a mediator of radiation-induced bystander effects. TGFB1 mRNA levels were detected using qRT-PCR in HeLa cells 2 h after exposure to different doses of X-ray irradiation. ( B ) TGFB1 mRNA levels were detected in HeLa cells exposed

    Techniques Used: Quantitative RT-PCR, Irradiation

    MiR-663 regulates RIBE mediated by TGF-β1. ( A ) Yields of 53BP1 foci in bystander HeLa cells cultured for 2 h in the medium harvested from HeLa cells with inducible miR-663 treated with/without tetracycline for 24 h and post-incubated
    Figure Legend Snippet: MiR-663 regulates RIBE mediated by TGF-β1. ( A ) Yields of 53BP1 foci in bystander HeLa cells cultured for 2 h in the medium harvested from HeLa cells with inducible miR-663 treated with/without tetracycline for 24 h and post-incubated

    Techniques Used: Cell Culture, Incubation

    6) Product Images from "Transforming growth factor ?1-induced astrocyte migration is mediated in part by activating 5-lipoxygenase and cysteinyl leukotriene receptor 1"

    Article Title: Transforming growth factor ?1-induced astrocyte migration is mediated in part by activating 5-lipoxygenase and cysteinyl leukotriene receptor 1

    Journal: Journal of Neuroinflammation

    doi: 10.1186/1742-2094-9-145

    Effects of a 5-LOX inhibitor and CysLT receptor antagonists on TGF-β1-induced migration in astrocytes. ( A ) Photomicrographs showing TGF-β1 (10 ng/ml)-induced astrocyte migration 24 h after treatment with the 5-LOX inhibitor zileuton, the CysLT 1 R antagonist montelukast and the CysLT 2 R antagonist Bay cysLT2 (1 μM). Scale bar, 400 μm. ( B-D ) TGF-β1-induced migration inhibited by 0.01 to 5 μM zileuton ( B ) and montelukast ( C ), but not Bay cysLT2 ( D ). Data are reported as mean ± S.E.M.; n = 8; * P
    Figure Legend Snippet: Effects of a 5-LOX inhibitor and CysLT receptor antagonists on TGF-β1-induced migration in astrocytes. ( A ) Photomicrographs showing TGF-β1 (10 ng/ml)-induced astrocyte migration 24 h after treatment with the 5-LOX inhibitor zileuton, the CysLT 1 R antagonist montelukast and the CysLT 2 R antagonist Bay cysLT2 (1 μM). Scale bar, 400 μm. ( B-D ) TGF-β1-induced migration inhibited by 0.01 to 5 μM zileuton ( B ) and montelukast ( C ), but not Bay cysLT2 ( D ). Data are reported as mean ± S.E.M.; n = 8; * P

    Techniques Used: Migration

    Effect of TGF-β1 on expression of CysLT receptors in astrocytes. ( A, B ) RT-PCR and Western blotting results showing that the mRNA (A) and protein expression (B) of CysLT 1 R, but not CysLT 2 R, in astrocytes increased after exposure to 10 ng/ml TGF-β1 for 24 h. Data are reported as mean ± S.E.M.; n = 4; ** P
    Figure Legend Snippet: Effect of TGF-β1 on expression of CysLT receptors in astrocytes. ( A, B ) RT-PCR and Western blotting results showing that the mRNA (A) and protein expression (B) of CysLT 1 R, but not CysLT 2 R, in astrocytes increased after exposure to 10 ng/ml TGF-β1 for 24 h. Data are reported as mean ± S.E.M.; n = 4; ** P

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

    TGF-β1 induces 5-LOX expression and translocation, and increases the production of CysLTs in astrocytes. ( A, B ) RT-PCR and Western blotting results showing that 5-LOX mRNA (A) and protein expression (B) were increased in astrocytes after exposure to 10 ng/ml TGF-β1 for 24 h. Results are expressed as the ratios to β-actin (A) or GAPDH (B). C, control; T, TGF-β1. ( C ) Immunocytochemical examination reveals 5-LOX translocation to the nuclear envelope (arrows) in astrocytes after exposure to 10 ng/ml TGF-β1. Scale bar, 50 μm. ( D ) ELISA shows the production of CysLTs was significantly increased with a peak at 12 h after exposure to 10 ng/ml TGF-β1. Data are reported as mean ± S.E.M.; n = 4 (A and B) 8 (D); ** P
    Figure Legend Snippet: TGF-β1 induces 5-LOX expression and translocation, and increases the production of CysLTs in astrocytes. ( A, B ) RT-PCR and Western blotting results showing that 5-LOX mRNA (A) and protein expression (B) were increased in astrocytes after exposure to 10 ng/ml TGF-β1 for 24 h. Results are expressed as the ratios to β-actin (A) or GAPDH (B). C, control; T, TGF-β1. ( C ) Immunocytochemical examination reveals 5-LOX translocation to the nuclear envelope (arrows) in astrocytes after exposure to 10 ng/ml TGF-β1. Scale bar, 50 μm. ( D ) ELISA shows the production of CysLTs was significantly increased with a peak at 12 h after exposure to 10 ng/ml TGF-β1. Data are reported as mean ± S.E.M.; n = 4 (A and B) 8 (D); ** P

    Techniques Used: Expressing, Translocation Assay, Reverse Transcription Polymerase Chain Reaction, Western Blot, Enzyme-linked Immunosorbent Assay

    Effects of LTD 4 and NMLTC 4 on TGF-β1 mRNA expression and TGF-β1 release in astrocytes. ( A ), RT-PCR analysis showing mRNA expression of TGF-β1 after treatment with 1, 10 and 100 nM LTD 4 or NMLTC 4 for 24 h. There was no significant difference in TGF-β1 expression after treatment with LTD 4 and NMLTC 4 ( n = 4 for each group, P > 0.05). ( B ) TGF-β1 release in the culture supernatants after exposure to LTD 4 and NMLTC 4 measured by ELISA. Neither LTD 4 nor NMLTC 4 affected TGF-β1 expression and release. Data are reported as mean ± S.E.M.; n = 6.
    Figure Legend Snippet: Effects of LTD 4 and NMLTC 4 on TGF-β1 mRNA expression and TGF-β1 release in astrocytes. ( A ), RT-PCR analysis showing mRNA expression of TGF-β1 after treatment with 1, 10 and 100 nM LTD 4 or NMLTC 4 for 24 h. There was no significant difference in TGF-β1 expression after treatment with LTD 4 and NMLTC 4 ( n = 4 for each group, P > 0.05). ( B ) TGF-β1 release in the culture supernatants after exposure to LTD 4 and NMLTC 4 measured by ELISA. Neither LTD 4 nor NMLTC 4 affected TGF-β1 expression and release. Data are reported as mean ± S.E.M.; n = 6.

    Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Effect of LTD 4 on astrocyte migration and TGF-β1-induced migration and proliferation. ( A, B ), Photomicrographs showing astrocyte migration 24 h after treatment with LTD 4 (0.01 to 100 nmol/L) in the absence (A) or presence of TGF-β1 (1 ng/ml, B). Scale bars, 400 μm. ( C ) Data are reported as mean ± S.E.M.; n = 8; * P
    Figure Legend Snippet: Effect of LTD 4 on astrocyte migration and TGF-β1-induced migration and proliferation. ( A, B ), Photomicrographs showing astrocyte migration 24 h after treatment with LTD 4 (0.01 to 100 nmol/L) in the absence (A) or presence of TGF-β1 (1 ng/ml, B). Scale bars, 400 μm. ( C ) Data are reported as mean ± S.E.M.; n = 8; * P

    Techniques Used: Migration

    Time-dependent migration of live astrocytes after exposure to TGF-β1 and LTD 4 . Live astrocytes were continuously monitored under a videomicroscope after exposure to TGF-β1 or/and LTD 4 . ( A ) Representative images showing astrocyte migration traced by videomicroscopy at 6, 12, 18 and 24 h after scratching. Scale bar, 200 μm. ( B ) TGF-β1 and LTD 4 concentration- and time-dependently accelerated migration. When TGF-β1 (1 ng/ml) combined with LTD 4 (0.1 nM), the effect at 24 h was more potent than that of TGF-β1 or LTD 4 alone. Data are reported as mean ± S.E.M.; n = 3; * P
    Figure Legend Snippet: Time-dependent migration of live astrocytes after exposure to TGF-β1 and LTD 4 . Live astrocytes were continuously monitored under a videomicroscope after exposure to TGF-β1 or/and LTD 4 . ( A ) Representative images showing astrocyte migration traced by videomicroscopy at 6, 12, 18 and 24 h after scratching. Scale bar, 200 μm. ( B ) TGF-β1 and LTD 4 concentration- and time-dependently accelerated migration. When TGF-β1 (1 ng/ml) combined with LTD 4 (0.1 nM), the effect at 24 h was more potent than that of TGF-β1 or LTD 4 alone. Data are reported as mean ± S.E.M.; n = 3; * P

    Techniques Used: Migration, Concentration Assay

    Effect of CysLT 1 R siRNA on LTD 4 - and TGF-β1-induced migration in astrocytes. ( A, B ) RT-PCR and Western blotting results showing inhibition of CysLT 1 R, but not CysLT 2 R, mRNA ( A ) and protein expression ( B ) by CysLT 1 R siRNA but not by negative control (NC) siRNA. ( C ) Double immunofluorescence staining showing inhibition of CysLT 1 R protein expression by CysLT 1 R siRNA in GFAP-positive astrocytes. ( D ) Photomicrographs showing that the astrocyte migration induced by LTD 4 (1 and 10 nM) and TGF-β1 (1 and 10 ng/ml) was inhibited by CysLT 1 R siRNA (siRNA) but not by NC siRNA. ( E ) CysLT 1 R siRNA inhibited migration induced by LTD 4 and TGF-β1. Data are reported as mean ± S.E.M.; n = 4 (A and B) or 8 (E); * P
    Figure Legend Snippet: Effect of CysLT 1 R siRNA on LTD 4 - and TGF-β1-induced migration in astrocytes. ( A, B ) RT-PCR and Western blotting results showing inhibition of CysLT 1 R, but not CysLT 2 R, mRNA ( A ) and protein expression ( B ) by CysLT 1 R siRNA but not by negative control (NC) siRNA. ( C ) Double immunofluorescence staining showing inhibition of CysLT 1 R protein expression by CysLT 1 R siRNA in GFAP-positive astrocytes. ( D ) Photomicrographs showing that the astrocyte migration induced by LTD 4 (1 and 10 nM) and TGF-β1 (1 and 10 ng/ml) was inhibited by CysLT 1 R siRNA (siRNA) but not by NC siRNA. ( E ) CysLT 1 R siRNA inhibited migration induced by LTD 4 and TGF-β1. Data are reported as mean ± S.E.M.; n = 4 (A and B) or 8 (E); * P

    Techniques Used: Migration, Reverse Transcription Polymerase Chain Reaction, Western Blot, Inhibition, Expressing, Negative Control, Double Immunofluorescence Staining

    Effect of TGF-β1 on astrocyte migration and proliferation. ( A ) Photomicrographs showing migration after treatment with TGF-β1 (0.1 to 10 ng/ml) for 24 h. Scale bar, 400 μm. ( B, C ) Fluorescence intensity was determined by fluorescence activated cell sorting after CFSE labeling at 0 (baseline) and 24 h. Mean fluorescence intensity (MFI) at 24 h reduced compared with baseline (B), but did not change 24 h after treatment with TGF-β1 (0.1, 1 and 10 ng/ml, C). Data are reported as mean ± S.E.M.; n = 8 (A), 3 (B) or 9 (C); ** P
    Figure Legend Snippet: Effect of TGF-β1 on astrocyte migration and proliferation. ( A ) Photomicrographs showing migration after treatment with TGF-β1 (0.1 to 10 ng/ml) for 24 h. Scale bar, 400 μm. ( B, C ) Fluorescence intensity was determined by fluorescence activated cell sorting after CFSE labeling at 0 (baseline) and 24 h. Mean fluorescence intensity (MFI) at 24 h reduced compared with baseline (B), but did not change 24 h after treatment with TGF-β1 (0.1, 1 and 10 ng/ml, C). Data are reported as mean ± S.E.M.; n = 8 (A), 3 (B) or 9 (C); ** P

    Techniques Used: Migration, Fluorescence, FACS, Labeling

    Diagram showing the roles of TGF-β1 and 5-LOX/CysLT 1 R in induction of astrocyte migration. TGF-β1 activates 5-LOX to produce CysLTs; the latter activates CysLT 1 R. Meanwhile, it also up-regulates CysLT 1 R expression, which enhances the activity of CysLT 1 R. The activated CysLT 1 R mediates TGF-β1-induced astrocyte migration.
    Figure Legend Snippet: Diagram showing the roles of TGF-β1 and 5-LOX/CysLT 1 R in induction of astrocyte migration. TGF-β1 activates 5-LOX to produce CysLTs; the latter activates CysLT 1 R. Meanwhile, it also up-regulates CysLT 1 R expression, which enhances the activity of CysLT 1 R. The activated CysLT 1 R mediates TGF-β1-induced astrocyte migration.

    Techniques Used: Migration, Expressing, Activity Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats
    Article Snippet: The slides were visualized with diaminobenzidine (DAB; Dako, Glostrup, Denmark), counterstained with Mayer’s hematoxylin, dehydrated through increasing concentrations of alcohol, cleared in xylene, and mounted in neutral balsam (Sigma). .. Serum cytokine levels Serum levels of TGF-β1 were determined using the commercially available TGF-β1 ELISA kit according to the manufacturer’s instructions (Boster Biological Technology). .. The OD value was determined at 450 nm using an ELISA reader and calculated at the linear portion of the curve.

    Article Title: MiR-663 inhibits radiation-induced bystander effects by targeting TGFB1 in a feedback mode
    Article Snippet: Luciferase activity was measured 48 h later using the Dual Luciferase Reporter Assay System (Promega) with a Tecan Infinite M200 Pro microplate reader (Tecan). .. Human TGF-β1 ELISA kits were purchased from Boster Corporation (Boster). ..

    Article Title: 3,4-dihydroxyphenylethanol suppresses irradiation-induced pulmonary fibrosis in adult rats
    Article Snippet: The sections were incubated with surfactant protein-B (SPB) or α smooth muscle actin (α-SMA) (Boster Biological Technology, Wuhan, China) antibodies at 37°C for 2 h. Washing with PBS was followed by incubation with poly-peroxidase-conjugated anti-mouse/rabbit IgG for 20 min. Diaminobenzidine (Dako, Glostrup, Denmark) and Mayer’s hematoxylin were used for visualization and counterstaining of the slides. .. TGF-β1 ELISA kit (Boster Biological Technology) was used for determination of serum levels of TGF-β1. .. Serum levels of IL-6, IL-10, and TNF-α were measured using flow cytometric bead assays (BD™ CBA Flex Set; BD, Sparks, MD, USA).

    Article Title: Inflammation and Oxidative Stress in Deficit Schizophrenia
    Article Snippet: The ELISA kits provided for this study were used according to manufacturer’s instructions to determine inflammatory and antioxidant markers. .. The following kits were used: Human T-AOC ELISA kit, Bioassay Tech Lab, China (TAOC); Human PON1 ELISA kit, Bioassay Tech Lab, China (PON1); Human TGF-β1 ELISA kit, Boster Bio, CA, USA) (TGF-β1); Human IL-12 ELISA kit, Diaclone SAS, France (IL-12); Human IL-10 ELISA kit, Diaclone SAS, France (IL-10); Human IL-1β ELISA kit, Diaclone SAS, France (IL-1β); Human IL-17 ELISA kit, Diaclone SAS, France (IL-17); Human IFN-α ELISA kit, Diaclone SAS, France (IFN-α); Human IFN-γ ELISA kit, Diaclone SAS, France (IFN-γ); and Human TNF-α ELISA kit, Diaclone SAS, France (TNF-α). .. Statistical AnalysisStatistical tests were performed using version 17.0 for Windows of SPSS (Statistical Package for the Social Sciences).

    Article Title: The role of Nrf2/PIWIL2/purine metabolism axis in controlling radiation-induced lung fibrosis
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA)TGF-β1 protein levels were measured in cell culture supernatant by a commercial TGF-β1 ELISA kit (Boster, Wuhan, China) following the manufacturer’s instructions. .. The absorbance of samples was detected at 450 nm using a microplate reader (Bio-Tek, Vermont, USA).

    Article Title: Targeting CXCR4 reverts the suppressive activity of T-regulatory cells in renal cancer
    Article Snippet: Cytokine assay IFN-γ and TGF-β1 were measured by ELISA assay on the culture supernatant collected on day 5 from suppression experiments. .. In particular, cytokine concentration was assessed by Human IFN-gamma Instant ELISA (Bender MedSystems) and Human TGF-β1 ELISA kit (Boster Biological Technology Co). .. Samples were acquired by LB 940 Multimode Reader Mithras (Berthold Technologies).

    Article Title: Transforming growth factor ?1-induced astrocyte migration is mediated in part by activating 5-lipoxygenase and cysteinyl leukotriene receptor 1
    Article Snippet: The CysLTs (LTC4 , LTD4 and LTE4 ) in astrocyte supernatants were assayed using a commercial CysLT ELISA kit (Cayman Chemical Co., Ann Arbor, MI, USA) according to the manufacturer’s instructions and calculated as pg/mg protein. .. The TGF-β1 in the supernatants was assayed using a commercial TGF-β1 ELISA kit (Wuhan Boster Biological Technology Co., Ltd., Wuhan, China) according to the manufacturer’s instructions, and calculated as pg/ml. ..

    Article Title: Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation
    Article Snippet: .. The TGF-β1 ELISA kit was obtained from Boster Biotechnology Co. Ltd. (Wuhan, China). .. The levels of TGF-β1 in serum were determined by using the TGF-β1 ELISA kit according to the manufacturer’s protocol.

    Cell Culture:

    Article Title: The role of Nrf2/PIWIL2/purine metabolism axis in controlling radiation-induced lung fibrosis
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA)TGF-β1 protein levels were measured in cell culture supernatant by a commercial TGF-β1 ELISA kit (Boster, Wuhan, China) following the manufacturer’s instructions. .. The absorbance of samples was detected at 450 nm using a microplate reader (Bio-Tek, Vermont, USA).

    Concentration Assay:

    Article Title: Targeting CXCR4 reverts the suppressive activity of T-regulatory cells in renal cancer
    Article Snippet: Cytokine assay IFN-γ and TGF-β1 were measured by ELISA assay on the culture supernatant collected on day 5 from suppression experiments. .. In particular, cytokine concentration was assessed by Human IFN-gamma Instant ELISA (Bender MedSystems) and Human TGF-β1 ELISA kit (Boster Biological Technology Co). .. Samples were acquired by LB 940 Multimode Reader Mithras (Berthold Technologies).

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    Boster Bio tgf β1 elisa kit
    Emodin decreases the levels of serum transforming growth <t>factor-β1</t> <t>(TGF-β1)</t> and mRNA levels in liver tissues in the rat model. Serum and liver tissue were obtained from each treated rat. A: Levels of serum TGF-β1 were determined
    Tgf β1 Elisa Kit, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Emodin decreases the levels of serum transforming growth factor-β1 (TGF-β1) and mRNA levels in liver tissues in the rat model. Serum and liver tissue were obtained from each treated rat. A: Levels of serum TGF-β1 were determined

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Emodin protects rat liver from CCl4-induced fibrogenesis via inhibition of hepatic stellate cells activation

    doi: 10.3748/wjg.15.4753

    Figure Lengend Snippet: Emodin decreases the levels of serum transforming growth factor-β1 (TGF-β1) and mRNA levels in liver tissues in the rat model. Serum and liver tissue were obtained from each treated rat. A: Levels of serum TGF-β1 were determined

    Article Snippet: The TGF-β1 ELISA kit was obtained from Boster Biotechnology Co. Ltd. (Wuhan, China).

    Techniques:

    (A) The effect of epigallocatechin-3-gallate (EGCG) on the serum levels of transforming growth factor β1 (TGF-β1), (B) interleukin (IL)-6, (C) IL-10, and (D) tumor necrosis factor α (TNF-α) at 15, 30, 60 and 120 days post-irradiation. (A) Serum TGF-β1 levels were significantly lower (p

    Journal: International Journal of Molecular Medicine

    Article Title: The green tea extract epigallocatechin-3-gallate inhibits irradiation-induced pulmonary fibrosis in adult rats

    doi: 10.3892/ijmm.2014.1745

    Figure Lengend Snippet: (A) The effect of epigallocatechin-3-gallate (EGCG) on the serum levels of transforming growth factor β1 (TGF-β1), (B) interleukin (IL)-6, (C) IL-10, and (D) tumor necrosis factor α (TNF-α) at 15, 30, 60 and 120 days post-irradiation. (A) Serum TGF-β1 levels were significantly lower (p

    Article Snippet: Serum cytokine levels Serum levels of TGF-β1 were determined using the commercially available TGF-β1 ELISA kit according to the manufacturer’s instructions (Boster Biological Technology).

    Techniques: Irradiation

    TT-Tregs are more suppressive than PB and PT-Tregs in RCC patients (A) Demethylation rate of FOXP3 -TSDR in TT-Tregs and PB-Tregs versus HD-Tregs (2 TT-Tregs vs 4 PB-Tregs vs 3 HD respectively). (B) IFN-γ-Treg dependent ELISA and (C) TGF-β1-Treg dependent ELISA from 8 RCC patients.

    Journal: Oncotarget

    Article Title: Targeting CXCR4 reverts the suppressive activity of T-regulatory cells in renal cancer

    doi: 10.18632/oncotarget.20363

    Figure Lengend Snippet: TT-Tregs are more suppressive than PB and PT-Tregs in RCC patients (A) Demethylation rate of FOXP3 -TSDR in TT-Tregs and PB-Tregs versus HD-Tregs (2 TT-Tregs vs 4 PB-Tregs vs 3 HD respectively). (B) IFN-γ-Treg dependent ELISA and (C) TGF-β1-Treg dependent ELISA from 8 RCC patients.

    Article Snippet: In particular, cytokine concentration was assessed by Human IFN-gamma Instant ELISA (Bender MedSystems) and Human TGF-β1 ELISA kit (Boster Biological Technology Co).

    Techniques: Enzyme-linked Immunosorbent Assay

    The role of different PIWIL2 expression levels on the production of transforming growth factor-β1 (TGF-β1) in WI-38 cells after irradiation. PIWIL2 overexpression (PIWIL2-OE) and knockdown (PIWIL2-KD) were confirmed at the mRNA (A) and protein (B) levels by qRT-PCR and western blotting in WI-38 cells. The wild-type (WT) WI-38 cells were irradiated (IR) (6 Gy) in the presence or absence of 50 nM CDDO-Me. The PIWIL2-KD WI-38 cells were treated with 50 nM CDDO-Me following 6 Gy of IR. PIWIL2-OE WI-38 cells were only irradiated by 6 Gy X-rays. The expression levels of transforming growth factor-β1 (TGF-β1) in the culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA) (C). *P

    Journal: American Journal of Cancer Research

    Article Title: The role of Nrf2/PIWIL2/purine metabolism axis in controlling radiation-induced lung fibrosis

    doi:

    Figure Lengend Snippet: The role of different PIWIL2 expression levels on the production of transforming growth factor-β1 (TGF-β1) in WI-38 cells after irradiation. PIWIL2 overexpression (PIWIL2-OE) and knockdown (PIWIL2-KD) were confirmed at the mRNA (A) and protein (B) levels by qRT-PCR and western blotting in WI-38 cells. The wild-type (WT) WI-38 cells were irradiated (IR) (6 Gy) in the presence or absence of 50 nM CDDO-Me. The PIWIL2-KD WI-38 cells were treated with 50 nM CDDO-Me following 6 Gy of IR. PIWIL2-OE WI-38 cells were only irradiated by 6 Gy X-rays. The expression levels of transforming growth factor-β1 (TGF-β1) in the culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA) (C). *P

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA)TGF-β1 protein levels were measured in cell culture supernatant by a commercial TGF-β1 ELISA kit (Boster, Wuhan, China) following the manufacturer’s instructions.

    Techniques: Expressing, Irradiation, Over Expression, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

    The effects of PIWIL2 overexpression (PIWIL2-OE) and knockout (PIWIL2-KD) on the expression of purine metabolism rate-limiting enzymes were analyzed by qRT-PCR (A). The wild-type (WT) WI-38 cells were irradiated (6 Gy) in the presence or absence of 50 nM CDDO-Me. The PIWIL2-KD WI-38 cells were treated with 50 nM CDDO-Me following 6 Gy of irradiation (IR). The PIWIL2-OE WI-38 cells were irradiated (6 Gy) in the presence or absence of 5 mM mycophenolate mofetil (MMF). The WT WI-38 cells treated with CDDO-Me and MMF following 6 Gy X-ray exposure. The expression levels of transforming growth factor-β1 (TGF-β1) in the culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA) (B). *P

    Journal: American Journal of Cancer Research

    Article Title: The role of Nrf2/PIWIL2/purine metabolism axis in controlling radiation-induced lung fibrosis

    doi:

    Figure Lengend Snippet: The effects of PIWIL2 overexpression (PIWIL2-OE) and knockout (PIWIL2-KD) on the expression of purine metabolism rate-limiting enzymes were analyzed by qRT-PCR (A). The wild-type (WT) WI-38 cells were irradiated (6 Gy) in the presence or absence of 50 nM CDDO-Me. The PIWIL2-KD WI-38 cells were treated with 50 nM CDDO-Me following 6 Gy of irradiation (IR). The PIWIL2-OE WI-38 cells were irradiated (6 Gy) in the presence or absence of 5 mM mycophenolate mofetil (MMF). The WT WI-38 cells treated with CDDO-Me and MMF following 6 Gy X-ray exposure. The expression levels of transforming growth factor-β1 (TGF-β1) in the culture supernatant were detected by enzyme-linked immunosorbent assay (ELISA) (B). *P

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA)TGF-β1 protein levels were measured in cell culture supernatant by a commercial TGF-β1 ELISA kit (Boster, Wuhan, China) following the manufacturer’s instructions.

    Techniques: Over Expression, Knock-Out, Expressing, Quantitative RT-PCR, Irradiation, Enzyme-linked Immunosorbent Assay

    Effects of different levels of Nrf2 on the production of transforming growth factor-β1 (TGF-β1) in WI-38 (A) and THP-1 (B) cells after irradiation. The wild-type (WT) cells were irradiated (IR) (6 Gy) in the presence or absence of 50 nM CDDO-Me. The irradiated Nrf2-KD cells were exposed to the same dose of X-ray as WT cells. The expression levels of TGF-β1 in the culture supernatant of WI-38 (A) and THP-1 (B) cells were detected by enzyme-linked immunosorbent assay (ELISA). *P

    Journal: American Journal of Cancer Research

    Article Title: The role of Nrf2/PIWIL2/purine metabolism axis in controlling radiation-induced lung fibrosis

    doi:

    Figure Lengend Snippet: Effects of different levels of Nrf2 on the production of transforming growth factor-β1 (TGF-β1) in WI-38 (A) and THP-1 (B) cells after irradiation. The wild-type (WT) cells were irradiated (IR) (6 Gy) in the presence or absence of 50 nM CDDO-Me. The irradiated Nrf2-KD cells were exposed to the same dose of X-ray as WT cells. The expression levels of TGF-β1 in the culture supernatant of WI-38 (A) and THP-1 (B) cells were detected by enzyme-linked immunosorbent assay (ELISA). *P

    Article Snippet: Enzyme-linked immunosorbent assay (ELISA)TGF-β1 protein levels were measured in cell culture supernatant by a commercial TGF-β1 ELISA kit (Boster, Wuhan, China) following the manufacturer’s instructions.

    Techniques: Irradiation, Expressing, Enzyme-linked Immunosorbent Assay