Human Tgfβ3 Picokine Elisa, supplied by Boster Bio, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Transient Mitomycin C-treatment of human corneal epithelial cells and fibroblasts alters cell migration, cytokine secretion, and matrix accumulation"
Article Title: Transient Mitomycin C-treatment of human corneal epithelial cells and fibroblasts alters cell migration, cytokine secretion, and matrix accumulation
Journal: Scientific Reports
Figure Legend Snippet: While qPCR and cytokine arrays show that MMC treatment elevates expression of several RNAs and proteins associated with cell migration and senescence, HCLE secretion of TGFβ1 and TGFβ3 are not altered by MMC treatment. ( A ) As indicated by the schematic, RNA was isolated from control and MMC treated cells allowed to recover overnight after a 3-hour MMC treatment. RNAs whose expression was found altered in human wound healing and cell migration RNA arrays were assessed by qPCR. Asterisks within bars indicate significant differences compared to untreated control cells indicated by the blue dashed line. While expression of MMP7 and MMP12 were not altered in MMC treated cells, expression of MMP1, CXCL1, PLAUR, VEGFA, and PTGS2 were elevated significantly with CXCL1 and PTGS2 both elevated more than 10-fold. Data were normalized against expression of the indicated RNA in control cells. ( B ) Conditioned media (CM) from equal numbers of control and MMC-treated HCLE cells was obtained as indicated in the schematic to determine whether secretion of cytokines is altered by MMC treatment. Cytokine arrays were performed twice using CM from cells obtained from 2 independent experiments. While most cytokines were below detectable, 8 were elevated significantly including CXCL10, G-CSF, GM-CSF, IL1α, IL1β, IL1ra, IL6, and IL8 and 3 were shown to not change in their secretion including CXCL1, MIF, and PAI-1. ( C ) TGFβ1 and TGFβ3 levels in E-CMC and E-CMM were quantified using ELISA assays. No significant differences were seen.
Techniques Used: Real-time Polymerase Chain Reaction, Expressing, Migration, Isolation, Enzyme-linked Immunosorbent Assay
Figure Legend Snippet: Conditioned media from MMC treated HCFs supports TGFβ1-induced collagen deposition but does not contain elevated levels of cytokines. ( A ) To determine whether exposure to proteins secreted by MMC-treated HCFs impacts collagen deposition by HCFs, F-CMC or F-CMM was added to HCF cultures with and without supplementation with vitamin C and TGFβ1 and collagen deposition assessed as shown schematically. Data show that the addition of vitamin C and TGFβ1 to both F-CMC and F-CMM significantly increases collagen deposition by HCFs. ( B ) Cytokine arrays were used to determine whether HCFs alter their secretion of cytokines into CM after MMC treatment. Data show that similar amounts of IL8, MIF, and PAI-1 are secreted into F-CMC and F-CMM and less CCL2 and CXCL1 is secreted into F-CMM. ( C ) To determine whether HCFs secrete different amounts of TGFβ1 and TGFβ3 into their media after MMC treatment, ELISA assays were performed on F-CMC and F-CMM without supplementation. No differences were observed for either growth factor.
Techniques Used: Enzyme-linked Immunosorbent Assay