Journal: American Journal of Cancer Research
Article Title: Tissue and serum IGFBP7 protein as biomarker in high-grade soft tissue sarcoma
Figure Lengend Snippet: IGFBP7 protein expression. A. Representative immunostaining of IGFBP7 in leiomyosarcoma (LMS) and undifferentiated pleomorphic sarcoma (UPS). A stronger expression is evident in metastatic patients, with predominant cytoplasmatic distribution (IHC 20 ×). B. Mann-Whitney analysis reveals statistically significant higher IGFBP7 staining levels in metastatic compared to non metastatic samples. C. Kaplan-Meier survival curves show the higher probability of the metastatic event in patients with high IGFBP7 expression (score 5-6).
Article Snippet: Individual serum samples (50 µl) were mixed with 450 µl dilution buffer and the concentration of IGFBP7 (ELISA kit from Boster Biological Technology Co., Fremont, CA, USA) was determined according to manufacturer’s instructions.
Techniques: Expressing, Immunostaining, Immunohistochemistry, MANN-WHITNEY, Staining