ek0864  (Boster Bio)


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    Name:
    Human IL 18 ELISA Kit PicoKine
    Description:

    Catalog Number:
    EK0864
    Price:
    400.0
    Category:
    ELISA Kits
    Reactivity:
    Human
    Applications:
    ELISA
    Immunogen:
    Expression system for standard: E.coli; Immunogen sequence: Y33-D197
    Buy from Supplier


    Structured Review

    Boster Bio ek0864
    Human IL 18 ELISA Kit PicoKine

    https://www.bioz.com/result/ek0864/product/Boster Bio
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ek0864 - by Bioz Stars, 2021-06
    95/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: H3 Relaxin Alleviates Migration, Apoptosis and Pyroptosis Through P2X7R-Mediated Nucleotide Binding Oligomerization Domain-Like Receptor Protein 3 Inflammasome Activation in Retinopathy Induced by Hyperglycemia
    Article Snippet: The plasma samples were used to detect IL-18, IL-1β, vascular endothelial growth factor (VEGF) and TNF-α concentrations with a Cytokine/Chemokine Magnetic Bead Panel kit (Cat#RECYTMAG-65K, EMD Millipore, Germany). .. Detection of Cytokines in the Culture MediumCulture medium was extracted by centrifuging at 1,000 g for 10 min, and IL-18 (CatEK0864, Bosterbio, Wuhan, China) and IL-1β (Cat#EK0392, Bosterbio, Wuhan, China) concentrations were measured by enzyme-linked immunosorbent assay. .. Western Blot Analysis Protein was harvested from rat retinal tissue and hRMECs using RIPA buffer with protease inhibitors, and the protein concentration was determined by the BCA protein assay (Beyotime, China).

    Article Title: A hydrogen peroxide responsive prodrug of Keap1-Nrf2 inhibitor for improving oral absorption and selective activation in inflammatory conditions
    Article Snippet: Then they were washed and treated with a DyLight 800 labeled secondary antibody at 37 °C for 2 h. The membranes were screened through the odyssey infrared imaging System (LI-COR, Lincoln, Nebraska, USA). .. 2.2.6 IL-1β, IL-18, IL-6, TNF-α and NO production Levels of IL-1β (IL-1β (m) ELISA kit, EK0394, Boster), IL-18 (IL-18 (m) ELISA kit, #EMC011, NeoBioscience), IL-6 (IL-6 (m) ELISA kit, EK0411, Boster), TNF-α (TNF-α (m) ELISA kit, EK0527, Boster) and NO production (Nitrate/Nitrite Assay Kit, S0023, Beyotime, China) were evaluated using commercially available kits according to the manufacturer's instructions. ..

    Article Title: miRNA-223-3p regulates NLRP3 to promote apoptosis and inhibit proliferation of hep3B cells
    Article Snippet: Cytokine detection The inflammatory cytokines IL-1β and IL-18 were detected in the cell culture supernatants. .. The Human IL-1 Beta PicoKine ELISA kit (cat no. EK0392) and the Human IL-18 PicoKine ELISA kit (cat no. EK0864; Boster Biological Technology, Wuhan, China) were used to measure the concentrations of the cytokines. .. Statistical analysis All data were analyzed by using SPSS software version 19.0 (IBM Corp., Armonk, NY, USA).

    Article Title: The effect of salusin-β on expression of pro- and anti-inflammatory cytokines in human umbilical vein endothelial cells (HUVECs)
    Article Snippet: These ELISA kits were specifically engineered for accurate and precise measurement protein levels of IL-6 and IL-8, respectively. .. The protein level of IL-18 was quantified with Human IL-18 Elisa kit, Boster Bio (catalog No: EK0864, USA), with a sensitivity ˂ 1 pg/ml. .. The protein level of IL-1Ra was determined by human IL-1Ra Elisa kit, Boster Bio (catalog No: EK0782, USA) with a sensitivity less than 2 pg/ml.

    Article Title: Human Cytomegalovirus Immediate Early 86-kDa Protein Blocks Transcription and Induces Degradation of the Immature Interleukin-1β Protein during Virion-Mediated Activation of the AIM2 Inflammasome
    Article Snippet: IL-1β was quantified using the BD OptEIA human IL-1β ELISA kit (BD Biosciences). .. IL-18 was quantified using the human IL-18 PicoKine ELISA kit (BosterBio). ..

    Article Title: CPUY192018, a potent inhibitor of the Keap1-Nrf2 protein-protein interaction, alleviates renal inflammation in mice by restricting oxidative stress and NF-κB activation
    Article Snippet: .. 2.12 IL-1β, IL-18, IL-6, TNF-α and NO production Levels of IL-1β (IL-1β (h) ELISA kit, EK0392, Boster), IL-18 (IL-18 (h) ELISA kit, EK0864, Boster), IL-6 (IL-6 (h) ELISA kit, EK0410, Boster), TNF-α (TNF-α (h) ELISA kit, EK0525, Boster) and NO production (Nitrate/Nitrite Assay Kit, S0023, Beyotime, China) were evaluated in human HK-2 cell culture supernatant using commercially available kits according to the manufacturer's instructions. .. 2.13 NF-κB localization by immunofluorescence The detection of NF-κB nuclear translocation was carried out following the instruction of the kit (NF-κB Activation, Nuclear Translocation Assay Kit, SN368, Beyotime, China).

    Article Title: Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release
    Article Snippet: The coverslips were observed using an Axiovert 200 (Zeiss) fluorescence microscope. .. Enzyme-Linked Immunosorbent Assay (ELISA) Supernatant was collected for the measurement of IL-1β and IL-18 concentration using ELISA kits (EK0864 and EK0392; Boster, Wuhan, China) according to manufacturer's instructions. .. Briefly, the supernatant of cell cultures was collected and IL-1β and IL-18 were determined by ELISA.

    Cell Culture:

    Article Title: EFLA 945 restricts AIM2 inflammasome activation by preventing DNA entry for psoriasis treatment.
    Article Snippet: Psoriasis is a chronic inflammatory skin disease that affects about 2% of the general population. .. Psoriasis is a chronic inflammatory skin disease that affects about 2% of the general population. ..

    Article Title: CPUY192018, a potent inhibitor of the Keap1-Nrf2 protein-protein interaction, alleviates renal inflammation in mice by restricting oxidative stress and NF-κB activation
    Article Snippet: .. 2.12 IL-1β, IL-18, IL-6, TNF-α and NO production Levels of IL-1β (IL-1β (h) ELISA kit, EK0392, Boster), IL-18 (IL-18 (h) ELISA kit, EK0864, Boster), IL-6 (IL-6 (h) ELISA kit, EK0410, Boster), TNF-α (TNF-α (h) ELISA kit, EK0525, Boster) and NO production (Nitrate/Nitrite Assay Kit, S0023, Beyotime, China) were evaluated in human HK-2 cell culture supernatant using commercially available kits according to the manufacturer's instructions. .. 2.13 NF-κB localization by immunofluorescence The detection of NF-κB nuclear translocation was carried out following the instruction of the kit (NF-κB Activation, Nuclear Translocation Assay Kit, SN368, Beyotime, China).

    Concentration Assay:

    Article Title: Antitumor Effects of Berberine on Gliomas via Inactivation of Caspase-1-Mediated IL-1β and IL-18 Release
    Article Snippet: The coverslips were observed using an Axiovert 200 (Zeiss) fluorescence microscope. .. Enzyme-Linked Immunosorbent Assay (ELISA) Supernatant was collected for the measurement of IL-1β and IL-18 concentration using ELISA kits (EK0864 and EK0392; Boster, Wuhan, China) according to manufacturer's instructions. .. Briefly, the supernatant of cell cultures was collected and IL-1β and IL-18 were determined by ELISA.

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  • 95
    Boster Bio il 18
    H3 relaxin alleviated NLRP3 inflammasome activation, apoptosis, pyroptosis and migration in hRMECs. (A) The protein levels of IL-1β and <t>IL-18</t> in cell culture media. (B) The protein expression of NLRP3 inflammasome and inflammatory cytolines were evaluated by western blot. (C) The protein expression of cleaved caspase-3, cleaved caspase-9, cleaved caspase-8 and GSDMD were evaluated by western blot. (D) The protein expression of VE-cadherin, ZO-1, occludin, MMP2 and MMP9 were evaluated by western blot. (E) The apoptosis in hRMECs were observed by transmission electron microscopy. (original magnification, ×5,000). Ultrastructural evaluation was performed: The majority of hRMECs treated by AGE-BSA showed apoptosis in early and middle phase. After H3 relaxin or/and insulin treatment, hRMECs were noticed improvement in early phase of apoptosis. Medullary corpuscles (arrow) can be noticed. (F,G) Cell apoptosis was measured by flow cytometry. (H,I) Wound-healing assay of hRMECs (Scale bar = 200 µm). (J,K) Transwell assay of hRMECs (Scale bar = 200 µm). Data are the means ± SD, and each measurement was repeated six times. ## p
    Il 18, supplied by Boster Bio, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/il 18/product/Boster Bio
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il 18 - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

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    H3 relaxin alleviated NLRP3 inflammasome activation, apoptosis, pyroptosis and migration in hRMECs. (A) The protein levels of IL-1β and IL-18 in cell culture media. (B) The protein expression of NLRP3 inflammasome and inflammatory cytolines were evaluated by western blot. (C) The protein expression of cleaved caspase-3, cleaved caspase-9, cleaved caspase-8 and GSDMD were evaluated by western blot. (D) The protein expression of VE-cadherin, ZO-1, occludin, MMP2 and MMP9 were evaluated by western blot. (E) The apoptosis in hRMECs were observed by transmission electron microscopy. (original magnification, ×5,000). Ultrastructural evaluation was performed: The majority of hRMECs treated by AGE-BSA showed apoptosis in early and middle phase. After H3 relaxin or/and insulin treatment, hRMECs were noticed improvement in early phase of apoptosis. Medullary corpuscles (arrow) can be noticed. (F,G) Cell apoptosis was measured by flow cytometry. (H,I) Wound-healing assay of hRMECs (Scale bar = 200 µm). (J,K) Transwell assay of hRMECs (Scale bar = 200 µm). Data are the means ± SD, and each measurement was repeated six times. ## p

    Journal: Frontiers in Pharmacology

    Article Title: H3 Relaxin Alleviates Migration, Apoptosis and Pyroptosis Through P2X7R-Mediated Nucleotide Binding Oligomerization Domain-Like Receptor Protein 3 Inflammasome Activation in Retinopathy Induced by Hyperglycemia

    doi: 10.3389/fphar.2020.603689

    Figure Lengend Snippet: H3 relaxin alleviated NLRP3 inflammasome activation, apoptosis, pyroptosis and migration in hRMECs. (A) The protein levels of IL-1β and IL-18 in cell culture media. (B) The protein expression of NLRP3 inflammasome and inflammatory cytolines were evaluated by western blot. (C) The protein expression of cleaved caspase-3, cleaved caspase-9, cleaved caspase-8 and GSDMD were evaluated by western blot. (D) The protein expression of VE-cadherin, ZO-1, occludin, MMP2 and MMP9 were evaluated by western blot. (E) The apoptosis in hRMECs were observed by transmission electron microscopy. (original magnification, ×5,000). Ultrastructural evaluation was performed: The majority of hRMECs treated by AGE-BSA showed apoptosis in early and middle phase. After H3 relaxin or/and insulin treatment, hRMECs were noticed improvement in early phase of apoptosis. Medullary corpuscles (arrow) can be noticed. (F,G) Cell apoptosis was measured by flow cytometry. (H,I) Wound-healing assay of hRMECs (Scale bar = 200 µm). (J,K) Transwell assay of hRMECs (Scale bar = 200 µm). Data are the means ± SD, and each measurement was repeated six times. ## p

    Article Snippet: Detection of Cytokines in the Culture MediumCulture medium was extracted by centrifuging at 1,000 g for 10 min, and IL-18 (Cat#EK0864, Bosterbio, Wuhan, China) and IL-1β (Cat#EK0392, Bosterbio, Wuhan, China) concentrations were measured by enzyme-linked immunosorbent assay.

    Techniques: Activation Assay, Migration, Cell Culture, Expressing, Western Blot, Transmission Assay, Electron Microscopy, Flow Cytometry, Wound Healing Assay, Transwell Assay

    Effect of H3 relaxin treatment on inflammation and apoptosis in retinas of diabetic rats. (A) The expression of IL-1β at 4 and 8 weeks in the plasma of diabetic rats. (B) The expression of IL-18 at 4 and 8 weeks in the plasma of diabetic rats. (C) The expression of TNF-α at 4 and 8 weeks in the plasma of diabetic rats. (D) The expression of VEGF at 4 and 8 weeks in the plasma of diabetic rats. (E) The protein expression of NLRP3 inflammasome and inflammatory cytolines at 4 and 8 weeks were evaluated by western blot. (F) The expression of apoptosis-related protein at 4 and 8 weeks were evaluated by western blot. Data are the means ± SD, and each measurement was repeated six times. # p

    Journal: Frontiers in Pharmacology

    Article Title: H3 Relaxin Alleviates Migration, Apoptosis and Pyroptosis Through P2X7R-Mediated Nucleotide Binding Oligomerization Domain-Like Receptor Protein 3 Inflammasome Activation in Retinopathy Induced by Hyperglycemia

    doi: 10.3389/fphar.2020.603689

    Figure Lengend Snippet: Effect of H3 relaxin treatment on inflammation and apoptosis in retinas of diabetic rats. (A) The expression of IL-1β at 4 and 8 weeks in the plasma of diabetic rats. (B) The expression of IL-18 at 4 and 8 weeks in the plasma of diabetic rats. (C) The expression of TNF-α at 4 and 8 weeks in the plasma of diabetic rats. (D) The expression of VEGF at 4 and 8 weeks in the plasma of diabetic rats. (E) The protein expression of NLRP3 inflammasome and inflammatory cytolines at 4 and 8 weeks were evaluated by western blot. (F) The expression of apoptosis-related protein at 4 and 8 weeks were evaluated by western blot. Data are the means ± SD, and each measurement was repeated six times. # p

    Article Snippet: Detection of Cytokines in the Culture MediumCulture medium was extracted by centrifuging at 1,000 g for 10 min, and IL-18 (Cat#EK0864, Bosterbio, Wuhan, China) and IL-1β (Cat#EK0392, Bosterbio, Wuhan, China) concentrations were measured by enzyme-linked immunosorbent assay.

    Techniques: Expressing, Western Blot